A highly versatile fungal glucosyltransferase for specific production of quercetin-7-O-β-d-glucoside and quercetin-3-O-β-d-glucoside in different hosts

Author(s):  
Jie Ren ◽  
Wenzhu Tang ◽  
Caleb Don Barton ◽  
Owen M. Price ◽  
Mark Wayne Mortensen ◽  
...  
Keyword(s):  
Author(s):  
Georgi Todorov ◽  
Krasimira Keremidchieva

On the go is the development and adaptation of an individual target for Bulgaria, in order to increase the share of RES energy, according to the EU common vision for leadership in the implementation of renewables to 32% in the gross final consumption by 2030. The technological progress, the easy applicability and feasibility of the projects, as well as the geographic and climatic specifics of Bulgaria, makes photovoltaic Bulgaria's main candidate for leading technology in this process. With net specific production of 1323kWh/annual per installed kWp, price levels of PV technology of €1360kWp and Levelized cost of energy (LCOE) below €0.12kWh for 20 years time frame, households in Bulgaria are at a stage where photovoltaic projects should be developed as an alternative to the grid supply


2020 ◽  
pp. 58-64
Author(s):  
V. V. Borodin ◽  
A. R. Sirakanyan

The study focuses on the procedure for appointing and conducting expert research in cases of crimes related to violations of labor protection and safety rules. The author out lines the range of research tasks in this area, lists the types of expertise that are widely used in this category of criminal cases. Based on the analysis of legal norms regulating the procedure for the appointment and conduct of forensic examinations, the author comes to the conclusion that the procedural legislation contains a significant number of General approaches to the appointment and conduct of expertise (including in the field of investigation of crimes related to violations of labor protection and safety rules). The specificity of expert research on this category of criminal cases is that in the framework of establishing the actual circumstances of the incident, it is often necessary to conduct several examinations (complex expert studies), taking into account the specifics of specific production processes at the enterprise.


2021 ◽  
Vol 7 (3) ◽  
pp. 445-467 ◽  
Author(s):  
Jan-Peter Herbst

Viking metal, Teutonic metal, Mesopotamian metal – labels of this kind are common in fan discourse, media and academia. Whereas some research has investigated such labels and related them to the artist’s stage presentation, music videos, artwork and lyrics, there is still a lack from the perspectives of music production and performance as to how such culturally and geographically associated labels differ musically. This article explores culture-specific production and performance characteristics of Teutonic metal, focusing on how metal from Germany differed from British and US-American productions in the 1980s and 1990s, during which time metal spread to Continental Europe and German speed metal achieved an international reputation for its original interpretation of metal. The study is based on a qualitative interview design with three record producers who were crucial for the rise of German metal labels and their bands: Harris Johns for Noise Records, Siggi Bemm for Century Media and Charlie Bauerfeind for Steamhammer. The findings suggest that performances differed between bands from Germany, America and Great Britain regarding timing, rhythmic precision, ensemble synchronization and expressiveness. Likewise, production approaches varied due to distinct preferences for certain guitar amplifiers, drum tunings, microphone techniques, mixing concepts and studio acoustics. Despite such culture-specific differences, it proved difficult for the interviewed producers to identify distinguishing features. Genre conventions seem to have a stronger impact than cultural origin overall.


2007 ◽  
Vol 70 (10) ◽  
pp. 2417-2421 ◽  
Author(s):  
ELEONORA DEHLINK ◽  
KONRAD J. DOMIG ◽  
CHRISTINE LOIBICHLER ◽  
ELKE KAMPL ◽  
THOMAS EIWEGGER ◽  
...  

The mode of inactivation of probiotic bacteria may profoundly affect their immune-modulatory properties to the point of reversal of effects in in vitro human intestinal epithelial-like cell cultures (Caco-2). To further investigate the influence of inactivation treatment on cytokine production, three probiotic strains were evaluated—live, heat-inactivated, and formalininactivated strains—for their impact on interleukin (IL) 6, IL-8, and IL-10 production in Caco-2–leucocyte cocultures. The tested bacteria induced strain-specific production of IL-6, IL-8, and IL-10. No suppressive effects on cytokine synthesis were observed. Live microorganisms seemed to be slightly more potent inducers of cytokine production than nonviable strains, but differences to inactivated bacteria were not statistically significant. Our results indicate that heat and formalin treatments of probiotic microorganisms are equivalent inactivation methods in terms of induction of IL-6, IL-8, and IL-10 production in Caco-2–peripheral blood mononuclear cell cocultures and do not invert immune-modulatory effects.


HortScience ◽  
2018 ◽  
Vol 53 (11) ◽  
pp. 1593-1599 ◽  
Author(s):  
Dave Hawley ◽  
Thomas Graham ◽  
Michael Stasiak ◽  
Mike Dixon

The influence of light spectral quality on cannabis (Cannabis sativa L.) development is not well defined. It stands to reason that tailoring light quality to the specific needs of cannabis may increase bud quality, consistency, and yield. In this study, C. sativa L. ‘WP:Med (Wappa)’ plants were grown with either no supplemental subcanopy lighting (SCL) (control), or with red/blue (“Red-Blue”) or red-green-blue (“RGB”) supplemental SCL. Both Red-Blue and RGB SCL significantly increased yield and concentration of total Δ9-tetrahydrocannabinol (Δ9-THC) in bud tissue from the lower plant canopy. In the lower canopy, RGB SCL significantly increased concentrations of α-pinine and borneol, whereas both Red-Blue and RGB SCL increased concentrations of cis-nerolidol compared with the control treatment. In the upper canopy, concentrations of α-pinine, limonene, myrcene, and linalool were significantly greater with RGB SCL than the control, and cis-nerolidol concentration was significantly greater in both Red-Blue and RGB SCL treated plants relative to the control. Red-Blue SCL yielded a consistently more stable metabolome profile between the upper and lower canopy than RGB or control treated plants, which had significant variation in cannabigerolic acid (CBGA) concentrations between the upper and lower canopies. Overall, both Red-Blue and RGB SCL treatments significantly increased yield more than the control treatment, RGB SCL had the greatest impact on modifying terpene content, and Red-Blue produced a more homogenous bud cannabinoid and terpene profile throughout the canopy. These findings will help to inform growers in selecting a production light quality to best help them meet their specific production goals.


Membranes ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 82
Author(s):  
Roy Nitzsche ◽  
Hendrik Etzold ◽  
Marlen Verges ◽  
Arne Gröngröft ◽  
Matthias Kraume

Hemicellulose and its derivatives have a high potential to replace fossil-based materials in various high-value-added products. Within this study, two purification cascades for the separation and valorization of hemicellulose and its derived monomeric sugars from organosolv beechwood hydrolyzates (BWHs) were experimentally demonstrated and assessed. Purification cascade 1 included hydrothermal treatment for converting remaining hemicellulose oligomers to xylose and the purification of the xylose by nanofiltration. Purification cascade 2 included the removal of lignin by adsorption, followed by ultrafiltration for the separation and concentration of hemicellulose. Based on the findings of the experimental work, both cascades were simulated on an industrial scale using Aspen Plus®. In purification cascade 1, 63% of the oligomeric hemicellulose was hydrothermally converted to xylose and purified by nanofiltration to 7.8 t/h of a xylose solution with a concentration of 200 g/L. In purification cascade 2, 80% of the lignin was removed by adsorption, and 7.6 t/h of a purified hemicellulose solution with a concentration of 200 g/L was obtained using ultrafiltration. The energy efficiency of the cascades was 59% and 26%, respectively. Furthermore, the estimation of specific production costs showed that xylose can be recovered from BWH at the cost of 73.7 EUR/t and hemicellulose at 135.1 EUR/t.


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