Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery

AbstractBallan wrasse (Labrus bergylta, Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon (Salmo salar, Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the microbiota of wrasse eggs has yet to be described. Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. Triplicate egg samples were collected from each of three spawning tanks and analysis of 16S rRNA gene sequences revealed that 88.6% of reads could be identified to 186 taxonomic families. At Day 0, reads corresponding to members of the Vibrionaceae, Colwelliaceae and Rubritaleaceae families were detected at greatest relative abundances. Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. At Day 5, there was a consistent reduction in 16S rRNA gene sequence richness across the tanks. Even though the eggs from the different tanks were incubated in a common holding tank, the bacterial communities of the eggs from the different tanks had diverged to become increasingly dissimilar. This suggests that the disinfection and incubation exerted differential effects of the microbiota of the eggs from each tank and that the influence of the tank water on the composition of the egg microbiota was lower than expected. This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. In future, mass DNA sequencing methods may be applied in hatcheries to screen for pathogens and as a tool to assess the health status of eggs.

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A Study on the Role and Function of Rehabilitation Counselor Using DACUM in Korea

Health & Welfare ◽

10.23948/kshw.2018.03.20.1.41 ◽

2018 ◽

Vol 20 (1) ◽

pp. 41-64

Author(s):

Chang-Hee Lee

Keyword(s):

Rehabilitation Counselor ◽

And Function ◽

Role And Function

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Identifikasi Peran dan Strategi Pelabuhan Bebas Sabang

Jurnal Rekayasa Hijau ◽

10.26760/jrh.v4i3.167-178 ◽

2020 ◽

Vol 4 (3) ◽

pp. 167-178

Author(s):

Zurayna Sari

Keyword(s):

Economic Development ◽

Descriptive Analysis ◽

Regional Growth ◽

Maintenance Strategy ◽

Regional Economic ◽

Growth Center ◽

Free Port ◽

And Function ◽

Role And Function

ABSTRAKPelabuhan berperan sebagai fasilitas penunjang pusat pertumbuhan regional dalam proses pembangunan ekonomi wilayah. Pelabuhan Bebas Sabang diarahkan sebagai pusat pertumbuhan ekonomi regional dan diharapkan dapat meningkatkan perekonomian Kawasan Sabang. Permasalahan yang dihadapi Pelabuhan Bebas Sabang adalah belum optimalnya peran dan fungsi Pelabuhan Bebas Sabang dalam menunjang perekonomian wilayah. Penelitian ini bertujuan untuk mengetahui peran Pelabuhan Bebas Sabang dalam mendorong perkembangan perekonomian Kawasan Sabang. Lingkup materi yang dibahas mencakup peran-peran Pelabuhan Bebas Sabang, menentukan potensi dan masalah serta upaya-upaya peningkatan peran Pelabuhan Bebas Sabang. Metode analisis yang dilakukan adalah analisis deskriptif dengan pendekatan analisis data kualitatif dan kuantitatif. Alat analisis yang digunakan adalah analisis SWOT IFAS-EFAS. Hasil analisis menunjukkan dalam kurun waktu 4 (empat) tahun terakhir dari tahun 2010-2013, Pelabuhan Bebas Sabang belum optimal dalam menjalankan perannya, sehingga membutuhkan strategi pengembangan dengan pendekatan Agressive Maintenance Strategy (strategi perbaikan agresif), yaitu strategi konsolidasi internal dengan memperbaiki faktor-faktor kelemahan untuk memaksimalkan pemanfaatan peluang.Kata kunci: Pengelolaan, SWOT IFAS-EFAS, WilayahABSTRACTPort was supporting facility of regional growth center in the process of regional economic development. Sabang free port was directed as the center of regional economic growth and expected to raise the economy of sabang. Problems faced by sabang free port was yet optimal role and function in supporting the economy of the region. This study aimed to determine the role of sabang free port in supporting the economic development of sabang. The covered material scope included roles of sabang free port, determining the potentials and problems and efforts of increasing the role of sabang free port. The method of analysis was descriptive analysis with qualitative and quantitative approach. The analytical tool used was the swot ifas-efas analysis. The analysis results showed in the period of 4 (four) years from 2010 until 2013, sabang free port was not optimal in carrying out its role yet, so it requires development strategies with agressive maintenance strategy approach, which is internal consolidation strategy by improving vulnerability factors to maximize the utilization of opportunities.Keywords:, Management, Regional, SWOT IFAS-EFAS

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Role and Function of Public Company Audit Committee

SSRN Electronic Journal ◽

10.2139/ssrn.2057148 ◽

2012 ◽

Author(s):

Gemma Quinsaat

Keyword(s):

Audit Committee ◽

Public Company ◽

And Function ◽

Role And Function

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SISTEM PENDUKUNG KEPUTUSAN ANALISIS KEPUASAN MAHASISWA TERHADAP PENGGUNAAN SARANA LABORATORIUM SISTEM INFORMASI

Jurnal Sains dan Informatika ◽

10.22216/jsi.v4i2.3735 ◽

2018 ◽

Vol 4 (2) ◽

pp. 150

Author(s):

Rahmadini Darwas

Keyword(s):

Service Quality ◽

Learning Process ◽

Quality Of Services ◽

Laboratory Facilities ◽

Cold Room ◽

And Function ◽

And Behavior ◽

Attitudes And Behavior ◽

Role And Function

Abstract Laboratory is one of the supporting facilities in im[roving the learning process. Problems found by students regarding the information system laboratory facilities at STMIK Indonesia Padang are the computers that suddenly die when operated, the less cold room, display data is blurred, making the inconvenience in the learning process that causes the students less satisfied with the services provided. Students will feel satisfied if the service is expected to match the received. Therefore, a decision support system is needed to analyze the quality of services provided to the students so that it can support the role and function of the laboratory optimally and what attributes need to be improved the quality of service. The method used is Fuzzy Service Quality (Servqual) method. The results showed that the service quality received was not in accordance with the expected because there is a gap of -1.55 for tangibles dimension. Attributes that need to be improved the quality of services are laboratory space is cool and comfortable, the use of laboratories relevant to the field of science, the responsibility of laboratory assistant, the availability of professional teachers and attitudes and behavior of labor officers. Abstrak Laboratorium merupakan salah satu fasilitas pendukung dalam meningkatkan proses pembelajaran. Permasalahan yang ditemukan mahasiswa mengenai fasilitas laboratorium sistem informasi pada STMIK Indonesia Padang adalah komputer yang tiba-tiba mati saat dioperasikan, ruangan yang kurang dingin, data display yang buram sehingga membuat ketidaknyamanan dalam proses pembelajaran yang menyebabkan mahasiswa kurang puas terhadap layanan yang diberikan. Mahasiswa akan merasa puas apabila layanan yang diharapkan sesuai dengan yang diterima. Oleh sebab itu, diperlukan suatu sistem pendukung keputusan untuk menganalisis kualitas layanan yang diberikan kepada mahasiswa sehingga dapat mendukung peran dan fungsi laboratorium secara optimal serta atribut apa saja yang perlu ditingkatkan kualitas layanannya. Metode yang digunakan adalah metode Fuzzy Service Quality (Servqual). Hasil penelitian menunjukkan bahwa kualitas layanan yang diterima belum sesuai dengan yang diharapkan karena terdapat gap sebesar -1.55 untuk dimensi tangibles. Atribut yang perlu ditingkatkan kualitas layanannya yaitu ruangan laboratorium yang sejuk dan nyaman, penggunaan laboratorium yang relevan dengan bidang ilmu, tanggungjawab asisten labor, tersedianya tenaga pengajar yang professional dan sikap serta perilaku petugas laborKata kunci : sistem pendukung keputusan, laboratorium, fuzzy, servqual

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Ancient Ways

10.1093/oso/9780190671181.003.0003 ◽

2018 ◽

Author(s):

Jonathan Weinel

Keyword(s):

Native American ◽

Visual Art ◽

Belief Systems ◽

Altered State ◽

Field Recordings ◽

Spirit World ◽

Relevant Field ◽

Altered State Of Consciousness ◽

And Function ◽

Role And Function

This chapter discusses shamanism, explaining the ethos and mythology of several indigenous societies, and how these belief systems relate to the design of art and music. First, a general overview of shamanism is provided, which outlines the typical role and function of a shaman. An explanation of the shamanic visionary experience, a type of altered state of consciousness, is then provided. Following this, the chapter explores a variety of visual art from indigenous shamanic cultures, including examples from San, Native American, Huichol, Tukano, and Shipibo traditions. The sound and music of shamanic and trance cultures is also discussed, with reference to Vodou, Tukano, Mazatec, Kiowa, and Mayan examples, and relevant field recordings. Through the course of this discussion, the chapter establishes a view of how shamanic art and music invoke a sense of the spirit world, which informs the subsequent discourse of Inner Sound.

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Beyond Bias

The Oxford Handbook of Ethics of AI ◽

10.1093/oxfordhb/9780190067397.013.47 ◽

2020 ◽

pp. 736-753

Author(s):

Chelsea Barabas

Keyword(s):

Best Practices ◽

Criminal Law ◽

Criminal Justice Reform ◽

Trade Offs ◽

System P ◽

Accuracy Measures ◽

Criminal Legal System ◽

And Function ◽

Role And Function

This chapter discusses contemporary debates regarding the use of artificial intelligence as a vehicle for criminal justice reform. It closely examines two general approaches to what has been widely branded as “algorithmic fairness” in criminal law: the development of formal fairness criteria and accuracy measures that illustrate the trade-offs of different algorithmic interventions; and the development of “best practices” and managerialist standards for maintaining a baseline of accuracy, transparency, and validity in these systems. Attempts to render AI-branded tools more accurate by addressing narrow notions of bias miss the deeper methodological and epistemological issues regarding the fairness of these tools. The key question is whether predictive tools reflect and reinforce punitive practices that drive disparate outcomes, and how data regimes interact with the penal ideology to naturalize these practices. The chapter then calls for a radically different understanding of the role and function of the carceral state, as a starting place for re-imagining the role of “AI” as a transformative force in the criminal legal system.

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A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils

International Journal of Molecular Sciences ◽

10.3390/ijms22073438 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3438

Author(s):

Juan Liu ◽

Xiangwei He ◽

Jingya Sun ◽

Yuchao Ma

Keyword(s):

Soil Fertility ◽

Microbial Diversity ◽

Resistance Genes ◽

Bacterial Communities ◽

Rhizosphere Soil ◽

Antibiotics Resistance ◽

Plant Growth Promoting Bacteria ◽

Soil Microbial Diversity ◽

Antibiotic Target ◽

And Function

Bacterial communities associated with roots influence the health and nutrition of the host plant. However, the microbiome discrepancy are not well understood under different healthy conditions. Here, we tested the hypothesis that rhizosphere soil microbial diversity and function varies along a degeneration gradient of poplar, with a focus on plant growth promoting bacteria (PGPB) and antibiotic resistance genes. Comprehensive metagenomic analysis including taxonomic investigation, functional detection, and ARG (antibiotics resistance genes) annotation revealed that available potassium (AK) was correlated with microbial diversity and function. We proposed several microbes, Bradyrhizobium, Sphingomonas, Mesorhizobium, Nocardioides, Variovorax, Gemmatimonadetes, Rhizobacter, Pedosphaera, Candidatus Solibacter, Acidobacterium, and Phenylobacterium, as candidates to reflect the soil fertility and the plant health. The highest abundance of multidrug resistance genes and the four mainly microbial resistance mechanisms (antibiotic efflux, antibiotic target protection, antibiotic target alteration, and antibiotic target replacement) in healthy poplar rhizosphere, corroborated the relationship between soil fertility and microbial activity. This result suggested that healthy rhizosphere soil harbored microbes with a higher capacity and had more complex microbial interaction network to promote plant growing and reduce intracellular levels of antibiotics. Our findings suggested a correlation between the plant degeneration gradient and bacterial communities, and provided insight into the role of high-turnover microbial communities as well as potential PGPB as real-time indicators of forestry soil quality, and demonstrated the inner interaction contributed by the bacterial communities.

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Climate Mobility and Development Cooperation

Population and Environment ◽

10.1007/s11111-021-00387-5 ◽

2021 ◽

Author(s):

Robert Stojanov ◽

Sarah Rosengaertner ◽

Alex de Sherbinin ◽

Raphael Nawrotzki

Keyword(s):

Climate Adaptation ◽

Human Mobility ◽

Climate Impacts ◽

Development Cooperation ◽

Climate Variability And Change ◽

Policy Frameworks ◽

And Migration ◽

And Function ◽

Role And Function

AbstractDevelopment cooperation actors have been addressing climate change as a cross-cutting issue and investing in climate adaptation projects since the early 2000s. More recently, as concern has risen about the potential impacts of climate variability and change on human mobility, development cooperation actors have begun to design projects that intentionally address the drivers of migration, including climate impacts on livelihoods. However, to date, we know little about the development cooperation’s role and function in responding to climate related mobility and migration. As such, the main aim of this paper is to outline the policy frameworks and approaches shaping development cooperation actors’ engagement and to identify areas for further exploration and investment. First, we frame the concept of climate mobility and migration and discuss some applicable policy frameworks that govern the issue from various perspectives; secondly, we review the toolbox of approaches that development cooperation actors bring to climate mobility; and third, we discuss the implications of the current Covid-19 pandemic and identify avenues for the way forward. We conclude that ensuring safe and orderly mobility and the decent reception and long-term inclusion of migrants and displaced persons under conditions of more severe climate hazards, and in the context of rising nationalism and xenophobia, poses significant challenges. Integrated approaches across multiple policy sectors and levels of governance are needed. In addition to resources, development cooperation actors can bring data to help empower the most affected communities and regions and leverage their convening power to foster more coordinated approaches within and across countries.

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Molecular characterization of the bacterial communities present in sheep's milk and cheese produced in South Brazilian Region via 16S rRNA gene metabarcoding sequencing

LWT ◽

10.1016/j.lwt.2021.111579 ◽

2021 ◽

Vol 147 ◽

pp. 111579

Author(s):

Creciana M. Endres ◽

Ícaro Maia S. Castro ◽

Laura D. Trevisol ◽

Juliana M. Severo ◽

Michele B. Mann ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Molecular Characterization ◽

Bacterial Communities ◽

Rrna Gene

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Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia

Scientific Reports ◽

10.1038/s41598-021-84031-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Lin Kang ◽

Pengtao Li ◽

Danyang Wang ◽

Taihao Wang ◽

Dong Hao ◽

...

Keyword(s):

Skeletal Muscle ◽

16S Rrna ◽

Intestinal Microbiota ◽

Muscle Growth ◽

Growth Function ◽

16S Rrna Sequencing ◽

Fecal Samples ◽

Lipopolysaccharide Biosynthesis ◽

Rrna Sequencing ◽

And Function

Abstract16S rRNA sequencing of human fecal samples has been tremendously successful in identifying microbiome changes associated with both aging and disease. A number of studies have described microbial alterations corresponding to physical frailty and nursing home residence among aging individuals. A gut-muscle axis through which the microbiome influences skeletal muscle growth/function has been hypothesized. However, the microbiome has yet to be examined in sarcopenia. Here, we collected fecal samples of 60 healthy controls (CON) and 27 sarcopenic (Case)/possibly sarcopenic (preCase) individuals and analyzed the intestinal microbiota using 16S rRNA sequencing. We observed an overall reduction in microbial diversity in Case and preCase samples. The genera Lachnospira, Fusicantenibacter, Roseburia, Eubacterium, and Lachnoclostridium—known butyrate producers—were significantly less abundant in Case and preCase subjects while Lactobacillus was more abundant. Functional pathways underrepresented in Case subjects included numerous transporters and phenylalanine, tyrosine, and tryptophan biosynthesis suggesting that protein processing and nutrient transport may be impaired. In contrast, lipopolysaccharide biosynthesis was overrepresented in Case and PreCase subjects suggesting that sarcopenia is associated with a pro-inflammatory metagenome. These analyses demonstrate structural and functional alterations in the intestinal microbiota that may contribute to loss of skeletal muscle mass and function in sarcopenia.

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