Hydrogen Sulfide Maintains Good Nutrition and Delays Postharvest Senescence in Postharvest Tomato Fruits by Regulating Antioxidative Metabolism

2021 ◽  
Author(s):  
Ting-Ying Zhong ◽  
Gai-Fang Yao ◽  
Sha-Sha Wang ◽  
Ting-Ting Li ◽  
Ke-Ke Sun ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Tomato Fruits ◽  
Antioxidative Metabolism ◽  
Good Nutrition ◽  
Postharvest Senescence

scholarly journals Hydrogen Sulfide Maintained the Good Appearance and Nutrition in Post-harvest Tomato Fruits by Antagonizing the Effect of Ethylene

2020 ◽  
Vol 11 ◽  
Author(s):  
Gai-Fang Yao ◽  
Chuang Li ◽  
Ke-Ke Sun ◽  
Jun Tang ◽  
Zhong-Qin Huang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Tomato Fruits ◽  
Post Harvest

scholarly journals Hydrogen Sulfide Alleviates Postharvest Senescence of Grape by Modulating the Antioxidant Defenses

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Zhi-Jing Ni ◽  
Kang-Di Hu ◽  
Chang-Bing Song ◽  
Run-Hui Ma ◽  
Zhi-Rong Li ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Hydrogen Sulfide ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Antioxidant Defenses ◽  
Carotenoid Content ◽  
Water Control ◽  
Chlorophyll Breakdown ◽  
S Application ◽  
Postharvest Senescence

Hydrogen sulfide (H2S) has been identified as an important gaseous signal in plants. Here, we investigated the mechanism of H2S in alleviating postharvest senescence and rotting of Kyoho grape. Exogenous application of H2S released from 1.0 mM NaHS remarkably decreased the rotting and threshing rate of grape berries. H2S application also prevented the weight loss in grape clusters and inhibited the decreases in firmness, soluble solids, and titratable acidity in grape pulp during postharvest storage. The data of chlorophyll and carotenoid content suggested the role of H2S in preventing chlorophyll breakdown and carotenoid accumulation in both grape rachis and pulp. In comparison to water control, exogenous H2S application maintained significantly higher levels of ascorbic acid and flavonoid and total phenolics and reducing sugar and soluble protein in grape pulp. Meanwhile, H2S significantly reduced the accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion (O2∙-) in grape pulp. Further investigations showed that H2S enhanced the activities of antioxidant enzymes ascorbate peroxidase (APX) and catalase (CAT) and decreased those of lipoxygenase (LOX) in both grape peels and pulp. In all, we provided strong evidence that H2S effectively alleviated postharvest senescence and rotting of Kyoho grape by modulating antioxidant enzymes and attenuating lipid peroxidation.

scholarly journals Hydrogen Sulfide Alleviates Senescence of Fresh-cut Apple by Regulating Antioxidant Defense System and Senescence-related Gene Expression

HortScience ◽  
2016 ◽  
Vol 51 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Ji-Lian Zheng ◽  
Lan-Ying Hu ◽  
Kang-Di Hu ◽  
Jun Wu ◽  
Feng Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Antioxidant Defense System ◽  
Guaiacol Peroxidase ◽  
Dependent Manner ◽  
Related Gene ◽  
Apple Slices ◽  
Apple Tissue ◽  
Fresh Cut ◽  
Postharvest Senescence

Hydrogen sulfide (H2S) has been identified as a multifunctional signaling molecule in plants. Here, we show that H2S delayed postharvest senescence of fresh-cut apples (Malus ×pumila) in a dose-dependent manner. Exogenous H2S application maintained significantly higher levels of ascorbic acid, flavonoids, total phenolics, reducing sugars and soluble proteins, and lower levels of free amino acids in apple slices compared with controls. Further investigations showed that H2S significantly reduced the accumulation of superoxide radicals, hydrogen peroxide (H2O2) and malondialdehyde (MDA). Apple fruits fumigated with H2S contained significantly higher activities of ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol peroxidase (POD) and superoxide dismutase (SOD), and lower activities of lipoxygenase (LOX), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and protease relative to controls. H2S also upregulated MdDHAR expression and downregulated the expression of MdLOX2, MdPG1, MdPPO, MdACO1, MdERS1, and MdETR1 in postharvest apple tissue. The present study indicates that H2S was involved in delaying postharvest senescence of apples by acting as an antioxidant and by regulating senescence-related gene expression.

scholarly journals The Antioxidant Defense System during Lanzhou Lily Scales Storage Is Modulated by Hydrogen Sulfide

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 183
Author(s):  
Changxia Li ◽  
Guibao Chen ◽  
Dengjing Huang ◽  
Ni Wang ◽  
Weibiao Liao
Keyword(s):  
Ascorbic Acid ◽  
Hydrogen Sulfide ◽  
Antioxidant Defense ◽  
Soluble Sugar ◽  
Total Phenolic ◽  
Sodium Hydrosulfide ◽  
Study Results ◽  
Antioxidant Defense Systems ◽  
Lanzhou Lily ◽  
Postharvest Senescence

As an important gaseous regulator, hydrogen sulfide (H2S) is involved in various aspects of plant processes, including seed germination, stomatal movement, and postharvest senescence. The preservation capacity of Lanzhou lily (Lilium davidii var. unicolor Salisb) scales fumigated with or without exogenously applied sodium hydrosulfide (NaHS, a H2S donor) was investigated in the current study. Results indicate that NaHS fumigation was able to extend storage life and elicit endogenous H2S production of postharvest Lanzhou lily scales with an optimal concentration at 0.8 mM. Moreover, exogenously applied NaHS (0.8 mM) led to higher soluble sugar, soluble protein, and ascorbic acid levels and lower total phenolic and flavonoid contents compared with those of the control. The application of 0.8 mM NaHS also reduced the lipid peroxidation level and reactive oxygen species (ROS) accumulation in scales, as indicated by the lower malondialdehyde (MDA) content, relative conductivity, lipoxygenase (LOX) activity, O2− production rate, and hydrogen per-oxide (H2O2) content. Further, scales treated with 0.8 mM NaHS exhibited significantly higher activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbic acid peroxidase (APX). Collectively, our data provide new insight into how the postharvest senescence of Lanzhou lily scales might be alleviated by H2S by enhancing antioxidant defense systems.

Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression

2014 ◽  
Vol 62 (5) ◽  
pp. 1119-1129 ◽  
Author(s):  
Shi-Ping Li ◽  
Kang-Di Hu ◽  
Lan-Ying Hu ◽  
Yan-Hong Li ◽  
An-Min Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Antioxidant Defense ◽  
Related Gene ◽  
Postharvest Senescence

Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Cell Model ◽  
Site Directed Mutagenesis ◽  
Critical Event ◽  
Hypertensive Rats ◽  
Pulmonary Artery Endothelial Cell

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.

Limnological characteristics, community metabolism and management strategies of a coastal sinkhole in Cuba (Cenote Jennifer)

2020 ◽  
Vol 56 ◽  
pp. 24
Author(s):  
Roberto González-De Zayas ◽  
Liosban Lantigua Ponce de León ◽  
Liezel Guerra Rodríguez ◽  
Felipe Matos Pupo ◽  
Leslie Hernández-Fernández
Keyword(s):  
Hydrogen Sulfide ◽  
Primary Productivity ◽  
Major Ions ◽  
Saline Water ◽  
Management Strategies ◽  
Tourist Destination ◽  
Community Metabolism ◽  
Morphological Studies ◽  
Made In ◽  
Limnological Parameters

The Cenote Jennifer is an important and unique aquatic sinkhole in Cayo Coco (Jardines del Rey Tourist Destination) that has brackish to saline water. Two samplings were made in 1998 and 2009, and 4 metabolism community experiments in 2009. Some limnological parameters were measured in both samplings (temperature, salinity, pH, dissolved oxygen major ions, hydrogen sulfide, nutrients and others). Community metabolism was measured through incubated oxygen concentration in clear and dark oxygen bottles. Results showed that the sinkhole limnology depends on rainfall and light incidence year, with some stratification episodes, due to halocline or oxycline presence, rather than thermocline. The sinkhole water was oligotrophic (total nitrogen of 41.5 ± 22.2 μmol l−1 and total phosphorus of 0.3 ± 0.2 μmol l−1) and with low productivity (gross primary productivity of 63.0 mg C m−2 d−1). Anoxia and hypoxia were present at the bottom with higher levels of hydrogen sulfide, lower pH and restricted influence of the adjacent sea (2 km away). To protect the Cenote Jennifer, tourist exploitation should be avoided and more resources to ecological and morphological studies should be allocated, and eventually use this aquatic system only for specialized diving. For conservation purposes, illegal garbage disposal in the surrounding forest should end.

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