scholarly journals Hydrogen Sulfide Alleviates Senescence of Fresh-cut Apple by Regulating Antioxidant Defense System and Senescence-related Gene Expression

HortScience ◽  
2016 ◽  
Vol 51 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Ji-Lian Zheng ◽  
Lan-Ying Hu ◽  
Kang-Di Hu ◽  
Jun Wu ◽  
Feng Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Antioxidant Defense System ◽  
Guaiacol Peroxidase ◽  
Dependent Manner ◽  
Related Gene ◽  
Apple Slices ◽  
Apple Tissue ◽  
Fresh Cut ◽  
Postharvest Senescence

Hydrogen sulfide (H2S) has been identified as a multifunctional signaling molecule in plants. Here, we show that H2S delayed postharvest senescence of fresh-cut apples (Malus ×pumila) in a dose-dependent manner. Exogenous H2S application maintained significantly higher levels of ascorbic acid, flavonoids, total phenolics, reducing sugars and soluble proteins, and lower levels of free amino acids in apple slices compared with controls. Further investigations showed that H2S significantly reduced the accumulation of superoxide radicals, hydrogen peroxide (H2O2) and malondialdehyde (MDA). Apple fruits fumigated with H2S contained significantly higher activities of ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol peroxidase (POD) and superoxide dismutase (SOD), and lower activities of lipoxygenase (LOX), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and protease relative to controls. H2S also upregulated MdDHAR expression and downregulated the expression of MdLOX2, MdPG1, MdPPO, MdACO1, MdERS1, and MdETR1 in postharvest apple tissue. The present study indicates that H2S was involved in delaying postharvest senescence of apples by acting as an antioxidant and by regulating senescence-related gene expression.

scholarly journals Hydrogen Sulfide Delays Postharvest Senescence and Plays an Antioxidative Role in Fresh-cut Kiwifruit

HortScience ◽  
2013 ◽  
Vol 48 (11) ◽  
pp. 1385-1392 ◽  
Author(s):  
Shuai-Ping Gao ◽  
Kang-Di Hu ◽  
Lan-Ying Hu ◽  
Yan-Hong Li ◽  
Yi Han ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Abiotic Stress Tolerance ◽  
Cell Movement ◽  
Guaiacol Peroxidase ◽  
Dependent Manner ◽  
Fruit Storage ◽  
Sodium Hydrosulfide ◽  
Root Organogenesis ◽  
Postharvest Ripening ◽  
Fresh Cut

Hydrogen sulfide (H2S) was recently recognized as an endogenous gaseous molecule involved in seed germination, root organogenesis, abiotic stress tolerance, guard cell movement, and delay of senescence in plants. In the present study, we show that H2S participates in the regulation of postharvest ripening and senescence in fresh-cut kiwifruit, Actinidia deliciosa. Fumigation of fresh-cut kiwifruit with the H2S donor sodium hydrosulfide (NaHS) solution prolonged kiwifruit storage time and alleviated senescence and tissue softening in a dose-dependent manner at an optimal concentration of 1.0 mmol·L−1 NaHS. H2S treatment maintained higher levels of reducing sugars, soluble proteins, free amino acids, ascorbate, and chlorophyll and lowered carotenoid levels. H2S treatment also significantly decreased the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2) and superoxide anion (•O2−) during fruit storage compared with water controls. Furthermore, the activities of guaiacol peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were increased by H2S treatment, whereas the activity of lipoxygenase (LOX) was decreased compared with untreated controls. Taken together, these results suggest that H2S is involved in prolonging postharvest shelf life and plays an antioxidative role in fresh-cut kiwifruit.

Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression

2014 ◽  
Vol 62 (5) ◽  
pp. 1119-1129 ◽  
Author(s):  
Shi-Ping Li ◽  
Kang-Di Hu ◽  
Lan-Ying Hu ◽  
Yan-Hong Li ◽  
An-Min Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Antioxidant Defense ◽  
Related Gene ◽  
Postharvest Senescence

scholarly journals Hydrogen Sulfide Acts as a Fungicide to Alleviate Senescence and Decay in Fresh-cut Sweetpotato

HortScience ◽  
2014 ◽  
Vol 49 (7) ◽  
pp. 938-943 ◽  
Author(s):  
Jun Tang ◽  
Kang-Di Hu ◽  
Lan-Ying Hu ◽  
Yan-Hong Li ◽  
Yong-Sheng Liu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Ipomoea Batatas ◽  
Abiotic Stress Tolerance ◽  
Soft Rot ◽  
Optimal Concentration ◽  
Black Rot ◽  
Guaiacol Peroxidase ◽  
Dependent Manner ◽  
Sodium Hydrosulfide ◽  
Fresh Cut

Hydrogen sulfide (H2S) has been shown to be a gaseous molecule in the regulation of many processes in plants such as abiotic stress tolerance, root organogenesis, stomatal movement, and postharvest fruit senescence. We studied the role of H2S in the regulation of senescence and fungal decay in fresh-cut sweetpotato (Ipomoea batatas L., cv. Xushu 18) roots. H2S donor sodium hydrosulfide (NaHS) alleviated senescence in fresh-cut sweetpotato root tissue in a dose-dependent manner with the optimal concentration of 2.0 mmol·L−1 NaHS solution. At the optimal concentration of 2.0 mmol·L−1 NaHS, H2S fumigation maintained higher levels of reducing sugar in sweetpotato fresh-cut root. H2S treatment also significantly increased the activities of guaiacol peroxidase (POD) and decreased those of polyphenol oxidase (PPO) in sweetpotato during storage. Further investigation showed that H2S treatment maintained a lower level of lipoxygenase (LOX) activity compared with water control. Consistently, the accumulation of malondialdehyde (MDA) was reduced in H2S-treated groups. Three fungal pathogens, Rhizopus nigricans, Mucor rouxianus, and Geotrichum candidum, were isolated from sweetpotato tissue infected with black rot or soft rot. H2S fumigation at 1 to 2.5 mmol·L−1 NaHS resulted in effective inhibition of the three fungi when grown on medium. When the three fungi were inoculated on the surface of sweetpotato slices, H2S fumigation greatly reduced the percentage of fungal infection. In conclusion, these data suggest that H2S effectively alleviated the senescence and decay in sweetpotato slices and might be developed into a novel fungicide for reduction of black rot or soft rot in sweetpotato.

scholarly journals Survival and Growth of Listeria monocytogenes on Fresh-Cut Apple Slices and Its Interaction with Glomerella cingulata and Penicillium expansum

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 177-181 ◽  
Author(s):  
William S. Conway ◽  
Britta Leverentz ◽  
Robert A. Saftner ◽  
Wojciech J. Janisiewicz ◽  
Carl E. Sams ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Storage Temperature ◽  
Penicillium Expansum ◽  
Controlled Atmosphere ◽  
Glomerella Cingulata ◽  
Food Borne ◽  
Apple Slices ◽  
Apple Tissue ◽  
Fresh Cut ◽  
Pathogen Populations

The food-borne human pathogen Listeria monocytogenes survived and its populations increased on cv. Delicious apple slices at 10 or 20°C in air or controlled atmosphere of 0.5% O2 and 15% CO2, but did not grow at 5°C. Controlled atmosphere had no significant effect on the survival or growth of L. monocytogenes. The pathogen populations declined over time when grown in various concentrations of apple juice and the decline was greater as the concentration of the juice decreased. Populations of L. monocytogenes inoculated into decayed apple tissue continually increased on fruit decayed by Glomerella cingulata but did not survive after 5 days on fruit decayed by Penicillium expansum. The pH of the decayed area declined from pH 4.7 to 3.7 in the case of P. expansum, but in the case of G. cingulata the pH increased from pH 4.7 to 7.0. This pH modification may be responsible for affecting the growth of the food-borne pathogen. Storage temperature, as well as the absence of postharvest pathogens such as G. cingulata, is important for maintaining the safety of fresh-cut apples.

scholarly journals Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm (Bombyx mori) in Response to Hydrogen Sulfide Exposure

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1110
Author(s):  
Rui Zhang ◽  
Yu-Yao Cao ◽  
Juan Du ◽  
Kiran Thakur ◽  
Shun-Ming Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Transcriptome Analysis ◽  
Fat Body ◽  
Tca Cycle ◽  
Transcriptional Level ◽  
Dependent Manner ◽  
Transport Pathway ◽  
Shell Weight ◽  
Study Gene Expression

Hydrogen sulfide (H2S) has been recognized for its beneficial influence on physiological alterations. The development (body weight) and economic characteristics (cocoon weight, cocoon shell ratio, and cocoon shell weight) of silkworms were increased after continuous 7.5 µM H2S treatment. In the present study, gene expression changes in the fat body of silkworms at the 5th instar larvae in response to the H2S were investigated through comparative transcriptome analysis. Moreover, the expression pattern of significant differentially expressed genes (DEGs) at the 5th instar larvae was confirmed by quantitative real-time PCR (qRT-PCR) after H2S exposure. A total of 1200 (DEGs) was identified, of which 977 DEGs were up-regulated and 223 DEGs were down-regulated. Most of the DEGs were involved in the transport pathway, cellular community, carbohydrate metabolism, and immune-associated signal transduction. The up regulated genes under H2S exposure were involved in endocytosis, glycolysis/gluconeogenesis, the citrate cycle (TCA cycle), and the synthesis of fibroin, while genes related to inflammation were down-regulated, indicating that H2S could promote energy metabolism, the transport pathway, silk synthesis, and inhibit inflammation in the silkworm. In addition, the expression levels of these genes were increased or decreased in a time-dependent manner during the 5th instar larvae. These results provided insight into the effects of H2S on silkworms at the transcriptional level and a substantial foundation for understanding H2S function.

scholarly journals Human METTL7B Encodes an Alkyl Thiol Methyltransferase that Methylates Hydrogen Sulfide

2020 ◽  
Author(s):  
Benjamin J. Maldonato ◽  
Rheem A. Totah
Keyword(s):  
Gene Expression ◽  
Hydrogen Sulfide ◽  
Growth Cycle ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Thiol Compounds ◽  
Disease States ◽  
Catalytic Function ◽  
Thiol Methyltransferase ◽  
First Time

Summary Paragraph/AbstractMethyltransferase-like protein 7B (METTL7B) is implicated in tumor growth and progression while gene expression is upregulated in several different disease states such as rheumatoid arthritis and breast cancer. Yet, the catalytic function of METTL7B has not been characterized. Here we demonstrate that METTL7B encodes a protein that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to hydrogen sulfide (H2S) to form methanethiol (CH3SH). Several exogenous aliphatic thiols were also identified as substrates. Modulation of METTL7B gene expression in HepG2 and HeLa cell culture directly alters the methylation of captopril, a marker reaction of alkyl thiol methyltransferase (TMT) activity(1, 2). Furthermore, cloned and recombinantly expressed and purified METTL7B full length protein methylates several thiol compounds, including hydrogen sulfide, 7α-thiospironolactone, captopril, and L-penicillamine in a concentration dependent manner. Endogenous thiols such as glutathione and cysteine or classic probe substrates of other known small molecule S-, N-, and O- methyltransferases were not substrates for METTL7B. Our results unequivocally demonstrate, and for the first time, that METTL7B, a protein implicated in several disease states, is an alkyl thiol methyltransferase(3–5). Identifying the catalytic function of METTL7B will enable future pharmacological research in disease pathophysiology where METTL7B expression and H2S levels can potentially alter the redox state and growth cycle of cells.

Sign in / Sign up

Export Citation Format

Share Document