Lymphocyte subsets in peripheral blood of patients with psoriasis before and after treatment with leishmania antigens

PurposeRadiation therapy (RT) induces an immune response, but the relationship of this response with tumor type is not fully understood. This meta-analysis further elucidated this relationship by analyzing the changes in T lymphocyte subsets in different tumors before and after radiotherapy.MethodsWe searched English-language electronic databases including PubMed, EMBASE, and the Cochrane Library to collect studies on the changes in peripheral blood CD3+ T lymphocytes, CD4+ T lymphocytes, and CD8+ T lymphocytes before and after radiotherapy in tumor patients from January 2015 to April 2021. The quality of the included literature was evaluated using the NOS scale provided by the Cochrane Collaboration, and statistical software RevMan 5.4 was used to analyze the included literature. P<0.05 was considered to indicate statistical significance.ResultsA total of 19 studies in 16 articles involving 877 tumor patients were included. All data were collected within 1 month before or after radiotherapy. Meta-analysis showed that numbers of CD3+ T lymphocytes (SMD: -0.40; 95% CI [-0.75, -0.04]; p = 0.03) and CD4+ T lymphocytes (SMD: -0.43; 95% CI: [-0.85, -0.02]; p = 0.04) were significantly reduced after radiotherapy compared with before treatment, but there was no statistically significant difference for CD8+ T lymphocytes (SMD: 0.33; 95% CI: [-0.88, 0.74]; p = 0.12). Subgroup analysis showed that peripheral blood T lymphocytes decreased in head and neck cancer. However, in prostate cancer and breast cancer, there was no significant change in peripheral blood. 1 month after radiotherapy, it has a potential proliferation and activation effect on lymphocytes in esophageal cancer and lung cancer. The results showed that CD8+T lymphocytes increased in peripheral blood after SBRT. Radiotherapy alone reduced CD3+ T lymphocyte numbers.ConclusionsWithin 1 month of radiotherapy, patients have obvious immunological changes, which can cause apoptosis and reduction of T lymphocytes, and affect the balance of peripheral blood immune cells. The degree of immune response induced by radiotherapy differed between tumor types.

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Marginal periodontitis and the immune system. III. Differences in peripheral blood lymphocyte subsets before and after treatment in adult periodontitis patients.

Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) ◽

10.2329/perio.30.1040 ◽

1988 ◽

Vol 30 (4) ◽

pp. 1040-1046

Author(s):

Takeshi YAMANAKA ◽

Seishi DOI ◽

Kazunori TAJIMA ◽

Junya KANEHISA ◽

Hiroshi TAKEUCHI ◽

...

Keyword(s):

Immune System ◽

Peripheral Blood Lymphocyte ◽

Peripheral Blood ◽

Blood Lymphocyte ◽

Lymphocyte Subsets ◽

Before And After ◽

Marginal Periodontitis

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Changes of Peripheral Blood Lymphocyte Subtypes in Patients with End Stage Cancer Administered Localized Radiotherapy and Bojungikki-Tang

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/207613 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 10

Author(s):

A-Jin Lee ◽

Ho Jun Lee ◽

Jong-Dae Kim ◽

Hyun-Jung Jung ◽

Sung Hwa Bae ◽

...

Keyword(s):

T Cells ◽

B Cells ◽

Cd8 T Cells ◽

Peripheral Blood ◽

Lymphocyte Subsets ◽

Water Extract ◽

Immune Dysfunction ◽

Before And After ◽

The Absolute ◽

End Stage

Localized radiotherapy (RT) can cause immune dysfunction. Bojungikki-tang is known to restore immune function. We investigated the absolute counts and percentages of peripheral blood (PB) lymphocyte subtypes in end stage cancer patients before and after RT and after oral administration of Bojungikki-tang water extract (BJITE) and to evaluate the changes mediated by RT and BJITE. Absolute counts and percentages of lymphocyte and lymphocyte subsets were determined in whole blood using the TetraONE System (Beckman Coulter, USA). Flow cytometry results were compared before and after RT and after administration of BJITE. Absolute numbers of CD3+, CD4+, and CD8+ T cells and CD19+ B cells decreased significantly after RT (P<0.05). Absolute numbers of CD3-CD56+ cells did not change in both groups. No significant differences were observed in the absolute counts of lymphocyte subtypes before and after administration of BJITE or vitamin group. When BJITE group was compared with vitamin group, absolute numbers of CD19+ B cells increased. RT-induced decrease in T cells and B cells in PB suggests that immune deterioration occurs after RT. Administration of BJITE might be effective in the restoration of number of B cells.

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Faculty Opinions recommendation of Assessment of gene expression in peripheral blood using RNAseq before and after weight restoration in anorexia nervosa.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718021629.793480009 ◽

2013 ◽

Author(s):

Kelly Klump

Keyword(s):

Gene Expression ◽

Anorexia Nervosa ◽

Peripheral Blood ◽

Before And After ◽

Weight Restoration

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Clinical relevance of T lymphocyte subsets in pediatric Graves’ disease

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2020-0158 ◽

2020 ◽

Vol 33 (11) ◽

pp. 1425-1430

Author(s):

Ting Chen ◽

Linqi Chen ◽

Haojie Song ◽

Xiuli Chen ◽

Rongrong Xie ◽

...

Keyword(s):

T Cell ◽

Peripheral Blood ◽

Clinical Characteristics ◽

Hepatic Dysfunction ◽

Lymphocyte Subsets ◽

Cytotoxic T Cell ◽

Graves Disease ◽

T Lymphocyte Subsets ◽

Graves Orbitopathy ◽

Normal Alanine Aminotransferase

AbstractObjectivesGraves’ disease (GD) is an autoimmune disease involving intimate response of both T cells and B cells. Immunophenotyping of peripheral blood lymphocyte subsets in GD children with different clinical characteristics can provide further information of the pathogenesis of GD.MethodsWe studied the lymphocyte subsets in peripheral blood of 141 children with GD. We repeatedly divided the patients into two groups in accordance with different clinical characteristics (abnormal or normal alanine aminotransferase (ALT) levels, the presence or absence of Graves’ orbitopathy (GO), and the presence or absence of hematuria. Then we compared the lymphocyte subsets measurements between two paired groups.ResultsWe found that serum ALT levels correlated positively with CD3+CD8+ T cell percentages in children with GD. Moreover, we detected higher percentages of CD3−CD19+ cells and higher ratio of CD4/CD8 in patients with GO. However, no correlation was found between GO status and lymphocyte subsets after excluding confounding effect of TRAb. No difference of lymphocyte subset percentages was found between groups with or without hematuria.ConclusionsSerum ALT levels correlate positively with cytotoxic T cell percentages in the peripheral blood of children with GD. The cytotoxic T cell may play a role in the pathogenesis of hepatic dysfunction in children with GD.

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CD8+ T lymphocyte is a main source of interferon-gamma production in Takayasu’s arteritis

Scientific Reports ◽

10.1038/s41598-021-96632-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yan-Long Ren ◽

Tao-Tao Li ◽

Wei Cui ◽

Li-Min Zhao ◽

Na Gao ◽

...

Keyword(s):

Interferon Gamma ◽

T Lymphocyte ◽

Peripheral Blood ◽

Lymphocyte Subsets ◽

Takayasu’S Arteritis ◽

Control Group ◽

Takayasu's Arteritis ◽

Cd8 T Lymphocyte ◽

Healthy Control ◽

Ifn Γ

AbstractInterferon-gamma (IFN-γ) is a cytokine involved in the pathogenesis of Takayasu’s arteritis (TAK). However, the source of IFN-γ in TAK patients is not fully clear. We aimed to investigate the source of IFN-γ in TAK. 60 TAK patients and 35 health controls were enrolled. The lymphocyte subsets of peripheral blood were detected by flow cytometry, cytokines were detected by Bio-plex. The correlation among lymphocyte subsets, cytokines and disease activity indexes was analyzed by person correlation. The level of serum IFN-γ in TAK patients was significantly increased (P < 0.05). The percentage of CD3+IFN-γ+ cells in peripheral blood CD3+ cells was significantly higher in TAK patients than that of healthy control group (P = 0.002). A higher proportion of CD3+CD8+IFN-γ+ cells/CD3+IFN-γ+ cells (40.23 ± 11.98% vs 35.12 ± 11.51%, P = 0.049), and a significantly lower CD3+CD4+IFN-γ+/ CD3+CD8+IFN-γ+ ratio (1.34 ± 0.62% vs 1.80 ± 1.33%, P = 0.027) were showed in the TAK group than that of control group. The CD3+CD8+IFN-γ+/CD3+IFN-γ+ ratio was positively correlated with CD3+IFN-γ+cells/ CD3+cells ratio (r = 0.430, P = 0.001), serum IFN-γ level (r = 0.318, P = 0.040) and IL-17 level (r = 0.326, P = 0.031). It was negatively correlated with CD3+CD4+IFN-γ+/CD3+IFN-γ+ ratio (r = − 0.845, P < 0.001). IFN-γ secreted by CD3+CD8 + T cells is an important source of serum IFN-γ in TAK patients.

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