Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model

1999 ◽  
Vol 291 (7-8) ◽  
pp. 437-446 ◽  
Author(s):  
I. Moll ◽  
P. Houdek ◽  
S. Schäfer ◽  
U. Nuber ◽  
R. Moll
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Immunohistochemical Study ◽  
Culture Model ◽  
Skin Organ Culture

Translational Use of a Standardized Full Human Skin Organ Culture Model in Autoimmune Blistering Diseases

2019 ◽  
Vol 85 (1) ◽  
pp. e56
Author(s):  
Imke A. K. Burmester ◽  
Shirin Emtenani ◽  
Jan-Gerrit Johns ◽  
Ralf J. Ludwig ◽  
Christoph M. Hammers ◽  
...  
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Culture Model ◽  
Skin Organ Culture ◽  
Autoimmune Blistering Diseases

scholarly journals Autophagy Quantification and STAT3 Expression in a Human Skin Organ Culture Model for Innate Immunity to Herpes Zoster

2018 ◽  
Vol 9 ◽  
Author(s):  
Erin M. Buckingham ◽  
James Girsch ◽  
Wallen Jackson ◽  
Jeffrey I. Cohen ◽  
Charles Grose
Keyword(s):  
Innate Immunity ◽  
Herpes Zoster ◽  
Organ Culture ◽  
Human Skin ◽  
Culture Model ◽  
Skin Organ Culture

scholarly journals Lipid functions in skin: Differential effects of n-3 polyunsaturated fatty acids on cutaneous ceramides, in a human skin organ culture model

2017 ◽  
Vol 1859 (9) ◽  
pp. 1679-1689 ◽  
Author(s):  
Alexandra C. Kendall ◽  
Magdalena Kiezel-Tsugunova ◽  
Luke C. Brownbridge ◽  
John L. Harwood ◽  
Anna Nicolaou
Keyword(s):  
Fatty Acids ◽  
Organ Culture ◽  
Polyunsaturated Fatty Acids ◽  
Human Skin ◽  
Differential Effects ◽  
Culture Model ◽  
Skin Organ Culture

scholarly journals Vascular Endothelial Growth Factor Blockade Induces Dermal Endothelial Cell Apoptosis in a Clinically Relevant Skin Organ Culture Model

2020 ◽  
Vol 33 (3) ◽  
pp. 110-118
Author(s):  
Andrea Luengas-Martinez ◽  
Jonathan Hardman-Smart ◽  
David Rutkowski ◽  
Talveen S. Purba ◽  
Ralf Paus ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Organ Culture ◽  
Cell Apoptosis ◽  
Vascular Endothelial ◽  
Endothelial Cell Apoptosis ◽  
Culture Model ◽  
Endothelial Growth Factor ◽  
Skin Organ Culture

scholarly journals Kinetic Cytokine Secretion Profile of LPS-Induced Inflammation in the Human Skin Organ Culture

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 299 ◽  
Author(s):  
Raanan Gvirtz ◽  
Navit Ogen-Shtern ◽  
Guy Cohen
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Cytokine Secretion ◽  
Peak Time ◽  
Local Skin ◽  
The Impact ◽  
Skin Organ Culture

Several in vitro models that mimic different aspects of local skin inflammation exist. The use of ex vivo human skin organ culture (HSOC) has been reported previously. However, comprehensive evaluation of the cytokine secretory capacity of the system and its kinetics has not been performed. Objective: the aim of the current study was to investigate the levels and secretion pattern of key cytokine from human skin tissue upon lipopolysaccharide (LPS) stimulation. HSOC maintained in an air–liquid interface was used. Epidermal and tissue viability was monitored by MTT and Lactate Dehydrogenase (LDH) activity assay, respectively. Cytokine levels were examined by ELISA and multiplex array. HSOCs were treated without or with three different LPS subtypes and the impact on IL-6 and IL-8 secretion was evaluated. The compounds enhanced the secreted levels of both cytokines. However, differences were observed in their efficacy and potency. Next, a kinetic multiplex analysis was performed on LPS-stimulated explants taken from three different donors to evaluate the cytokine secretion pattern during 0–72 h post-induction. The results revealed that the pro-inflammatory cytokines IL-6, IL-8, TNFα and IL-1β were up-regulated by LPS stimuli. IL-10, an anti-inflammatory cytokine, was also induced by LPS, but exhibited a different secretion pattern, peak time and maximal stimulation values. IL-1α and IL-15 showed donor-specific changes. Lastly, dexamethasone attenuated cytokine secretion in five independent repetitions, supporting the ability of the system to be used for drug screening. The collective results demonstrate that several cytokines can be used as valid inflammatory markers, regardless of changes in the secretion levels due to donor’s specific alterations.

Bioconversion of tocopheryl acetate to tocopherol in human skin: Use of human skin organ culture models

1998 ◽  
Vol 5 ◽  
pp. 190
Author(s):  
Z. Nabi ◽  
A. Tavakkol ◽  
N. Soliman ◽  
T.G. Polefka
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Tocopheryl Acetate ◽  
Culture Models ◽  
Skin Organ Culture

Penetration and biological effects of topically applied cyclosporin A nanoparticles in a human skin organ culture inflammatory model

2012 ◽  
Vol 21 (12) ◽  
pp. 938-943 ◽  
Author(s):  
Marina Frušić-Zlotkin ◽  
Yoram Soroka ◽  
Ran Tivony ◽  
Liraz Larush ◽  
Lilian Verkhovsky ◽  
...  
Keyword(s):  
Organ Culture ◽  
Cyclosporin A ◽  
Human Skin ◽  
Biological Effects ◽  
Skin Organ Culture

Establishment of a 2-week canine skin organ culture model and its pharmacological modulation by epidermal growth factor and dexamethasone

2016 ◽  
Vol 207 ◽  
pp. 109-117 ◽  
Author(s):  
Francesca Abramo ◽  
Andrea Pirone ◽  
Carla Lenzi ◽  
Iacopo Vannozzi ◽  
Maria Federica della Valle ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Organ Culture ◽  
Pharmacological Modulation ◽  
Culture Model ◽  
Epidermal Growth ◽  
Skin Organ Culture
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