Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat

The RecA protein of E. coli has been shown to mediate genetic recombination, regulate its own synthesis, control the expression of other genes, act as a specific protease, form a helical polymer and have an ATPase activity, among other observed properties. The unusual filament formed by the RecA protein on DNA has not previously been shown to exist outside of bacteria. Within this filament, the 36 Å pitch of B-form DNA is extended to about 95 Å, the pitch of the RecA helix. We have now establishedthat similar nucleo-protein complexes are formed by bacteriophage and yeast proteins, and availableevidence suggests that this structure is universal across all of biology, including humans. Thus, understanding the function of the RecA protein will reveal basic mechanisms, in existence inall organisms, that are at the foundation of general genetic recombination and repair.Recombination at this moment is assuming an importance far greater than just pure biology. The association between chromosomal rearrangements and neoplasms has become stronger and stronger, and these rearrangements are most likely products of the recombinatory apparatus of the normal cell. Further, damage to DNA appears to be a major cause of cancer.

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The experience of holding the cycles of assisted reproductive technology with defrost, a biopsy, genetic study and refreezing of embryos in patients with multiple unsuccessful implantations

HEALTH OF WOMAN ◽

10.15574/hw.2016.113.166 ◽

2016 ◽

pp. 166-170

Author(s):

Y.V. Masliy ◽

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I.O. Sudoma ◽

P.S. Mazur ◽

D.A. Mykytenko ◽

...

Keyword(s):

Chromosomal Rearrangements ◽

Genetic Diagnosis ◽

Morphological Characteristics ◽

Implantation Rate ◽

Ovarian Response ◽

Reproductive Technologies ◽

Comparative Genome Hybridization ◽

Comparative Genome ◽

Vitro Fertilization

The objective: to study the possibility of using frozen blastocysts for biopsy and genetic testing and performance measurement transfer euploeded 5–7-day-old embryos after thawing, biopsies, refreezing and thawing in patients with unsuccessful implantation. Patients and methods. The object of the study was the group of patients with repeated failure of implantation (4) in programs of auxiliary reproductive technologies (ART), subject to transfer to the uterus in total (i.e. in all the programs) for at least 6 good quality embryos based on morphological characteristics). All women had sufficient ovarian reserve. The patient was treated for infertility within the ART programs of the clinic of reproductive medicine "Nadiya" in the period from 2006 to 2016. The sample included couples who were not carriers of chromosomal rearrangements, without anomalies of the uterus (congenital and acquired: a doubling of the uterus, one-horned uterus, intrauterine membrane, synechia, submucous myoma of the uterus). All women had a positive ovarian response to controlled stimulation with gonadotropins (at least 7 oocytes) and a sufficient number of cryopreserved embryos. The first group (G1) included 64 women who trophectodermal a biopsy was performed on fresh blastocysts (in a loop controlled ovarian hyperstimulation). The second group (G2) were included 31 women who underwent thawing previously cryopreserved blastocysts trophectodermal re-biopsy and vitrification of blastocysts. Results. It was found that the performance of transfers euploid embryos that were vitrified, bioptrone and revitriphted, a little lower than those that were bioptrone fresh and vitrified only once. At the same time computationa genetic diagnosis previously vitrified blastocysts using comparative genome hybridization in patients with recurrent failed implantation allows to obtain a reasonable pregnancy rate (58%), implantation rate (33.3 %) and the birth of living children (45.1 %). Conclusion. Reprising biopropane embryos does not cause significant destructive impact and allows you to achieve pregnancy and birth of the alive child. Key words: in vitro fertilization, reusable unsuccessful implantation, a method of comparative genome hybridization, refreezing.

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Super-scaffolding the fire ant genome and detection of chromosomal rearrangements

10.1603/ice.2016.109192 ◽

2016 ◽

Author(s):

Eckart Stolle

Keyword(s):

Chromosomal Rearrangements ◽

Fire Ant

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Effect of Protein Content on the Quantity and Size Distribution of Polymeric Protein in Common Wheat

Japanese Journal of Crop Science ◽

10.1626/jcs.80.77 ◽

2011 ◽

Vol 80 (1) ◽

pp. 77-83 ◽

Cited By ~ 1

Author(s):

Mikiko Yanaka ◽

Kanenori Takata ◽

Tatsuya M. Ikeda ◽

Naoyuki Ishikawa

Keyword(s):

Protein Content ◽

Size Distribution ◽

Common Wheat

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Effect of algae Ecklonia maxima extract (Kelpak SL) on yields of common wheat, durum wheat and spelt wheat

Agronomy Science ◽

10.24326/2019.1.1 ◽

2019 ◽

Vol 74 (1) ◽

pp. 5-14

Author(s):

GRZEGORZ SZUMIŁO ◽

LESZEK RACHOŃ ◽

BARBARA KROCHMAL-MARCZAK

Keyword(s):

Triticum Aestivum ◽

Durum Wheat ◽

Common Wheat ◽

Yield Components ◽

Grain Quality ◽

Foliar Application ◽

Control Treatment ◽

Favourable Effect ◽

Ecklonia Maxima ◽

Spelt Wheat

The 3-year experiment was concerned with the response of spring forms of common wheat (Triticum aestivum L. subsp. aestivum), durum wheat (Triticum durum Desf.) and spelt wheat (Triticum aestivum subsp. spelta L. em. Thell.) to the foliar application of a plant growth stimulant (extract from marine algae Ecklonia maxima), with the commercial name of Kelpak SL (GS), as compared to control treatment (C). The following parameters were analysed: yield of grain, yield components (number of ears, weight of 1000 kernels, number and weight of kernels per ear) and physical indicators of grain quality (test weight, uniformity and vitreosity of grain). The study showed that the level of yielding and the yield components were related primarily with the wheat genotype, but they depended also on the agro-climatic conditions and on the algae extract and control experimental treatments. The application of algae extract, compared to the control, caused a significant increase in the yields of the spring wheat species under study, on average by 7.0%. Canopy spraying with algae extract had a favourable effect on the number of ears, on he number and weight of kernels per ear, but it had no effect on the weight of 1000 kernels. The grain quality of durum wheat, spelt wheat and common wheat was affected more strongly by the weather conditions in the successive years of the study and by the genotype than by the foliar application of algae extract. The spelt genotypes were characterised by lower yields and lower grain quality than common wheat and the durum wheat genotypes.

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