The micro-CT evaluation of enamel-cement thickness, abrasion, and mineral density in teeth in the postmortem interval (PMI): new parameters for the determination of PMI

Introduction. Patients with surgical menopause have a risk for osteopenic syndrome (OS). Menopausal hormone therapy (MHT) in combination with calcium and vitamin D promotes increase in bone mineral density (BMD). The expression level of vitamin D receptor in mononuclear fraction cells (MNFC) of blood can be considered as a predictive marker of effectiveness of OS therapy. Aim. To search a molecular predictive marker of the effectiveness of OS treatment. Materials and methods. The study included 100 women aged 4055 years with a duration of surgical menopause from 12 months to 6 years. The criterion for including patients in the study was the absence of contraindications to the use of MHT. The subject of the study was the determination of BMD by dual-energy X-ray absorptiometry, polymerase chain reaction diagnostics of the level of expression of vitamin D genes, estradiol and progesterone receptors, determination of 25-OH vitamin D in the blood. Results. Analysis of 12-month OS therapy effectiveness evaluated with a surrogate marker BMD. The increase in BMD up to 34% per year was treated as absence of negative dynamics, more than 4% per year as positive one. Significant effect of combination therapy compared with MHT on BMD in patients with surgical menopause with a low baseline level of BMD (due to hypovitaminosis D) is associated with the anti-inflammatory, bone-protective effect of vitamin D. In both groups of patients not responding; to the prescribed therapy we were able to conduct a comparative analysis of expression level of the target molecules in the MNFC before the start of treatment. The efficacy of MHT and combination therapy for BMD disorders is positively associated with the expression level of vitamin D receptors in MNFC before treatment. Therefore, the vitDR mRNA level is a potential predictive marker of the effectiveness of OS treatment. The expression levels of nuclear estradiol beta receptor and membrane receptor for progesterone in MNFC before treatment showed an upward trend in women responding to therapy. Conclusion. The expression level of the vitamin D receptor in MNFC of blood is significantly lower in the group of women with no/insufficient effect on 12-month combined therapy. This indicator can be considered as a predictive marker of the effectiveness of OS therapy.

Download Full-text

Circulating bioactive sclerostin levels in an Austrian population-based cohort

Wiener klinische Wochenschrift ◽

10.1007/s00508-021-01815-0 ◽

2021 ◽

Author(s):

Katharina Kerschan-Schindl ◽

Ursula Föger-Samwald ◽

Andreas Gleiss ◽

Stefan Kudlacek ◽

Jacqueline Wallwitz ◽

...

Keyword(s):

Serum Levels ◽

Population Based ◽

Negative Regulator ◽

Total Serum ◽

Mineral Density ◽

Cross Sectional ◽

Robust Parameter ◽

Austrian Population ◽

Different Levels

Summary Background Circulating serum sclerostin levels are supposed to give a good estimation of the levels of this negative regulator of bone mass within bone. Most studies evaluating total serum sclerostin found different levels in males compared to females and in older compared to younger subjects. Besides an ELISA detecting total sclerostin an ELISA determining bioactive sclerostin has been developed. The aim of this study was to investigate serum levels of bioactive sclerostin in an Austrian population-based cohort. Methods We conducted a cross-sectional observational study in 235 healthy subjects. Using the bioactive ELISA assay (Biomedica) bioactive sclerostin levels were evaluated. Results Serum levels of bioactive sclerostin were higher in men than in women (24%). The levels correlated positively with age (r = 0.47). A positive correlation could also be detected with body mass index and bone mineral density. Conclusion Using the ELISA detecting bioactive sclerostin our results are consistent with data in the literature obtained by different sclerostin assays. The determination of sclerostin concentrations in peripheral blood thus appears to be a robust parameter of bone metabolism.

Download Full-text

Micro‐CT analysis of the Lisfranc complex reveals higher bone mineral density in dorsal compared to plantar regions

Journal of Orthopaedic Research® ◽

10.1002/jor.25164 ◽

2021 ◽

Author(s):

Melissa R. Requist ◽

Yantarat Sripanich ◽

Tim Rolvien ◽

Amy L. Lenz ◽

Alexej Barg

Keyword(s):

Bone Mineral Density ◽

Bone Mineral ◽

Micro Ct ◽

Mineral Density ◽

Ct Analysis

Download Full-text

An In Vivo 3D Micro-CT Evaluation of Tooth Movement After the Application of Different Force Magnitudes in Rat Molar

The Angle Orthodontist ◽

10.2319/071308-366.1 ◽

2009 ◽

Vol 79 (4) ◽

pp. 703-714 ◽

Cited By ~ 29

Author(s):

Carmen Gonzales ◽

Hitoshi Hotokezaka ◽

Yoshinori Arai ◽

Tadashi Ninomiya ◽

Junya Tominaga ◽

...

Keyword(s):

Tooth Movement ◽

Three Dimensional ◽

Element Model ◽

Longitudinal Change ◽

Micro Ct ◽

Linear Measurements ◽

Center Of Rotation ◽

Ct Evaluation ◽

Space Width

Abstract Objective: To investigate the precise longitudinal change in the periodontal ligament (PDL) space width and three-dimensional tooth movement with continuous-force magnitudes in living rats. Materials and Methods: Using nickel-titanium closed-coil springs for 28 days, 10-, 25-, 50-, and 100-g mesial force was applied to the maxillary left first molars. Micro-CT was taken in the same rat at 0, 1, 2, 3, 10, 14, and 28 days. The width of the PDL was measured in the pressure and tension sides from 0 to 3 days. Angular and linear measurements were used to evaluate molar position at day 0, 10, 14, and 28. The finite element model (FEM) was constructed to evaluate the initial stress distribution, molar displacement, and center of rotation of the molar. Results: The initial evaluation of PDL width showed no statistical differences among different force magnitudes. Tooth movement was registered 1 hour after force application and gradually increased with time. From day 10, greater tooth movement was observed when 10 g of force was applied. The FEM showed that the center of rotation in the molar is located in the center of five roots at the apical third of the molar roots. Conclusion: The rat's molar movement mainly consists of mesial tipping, extrusion of distal roots, intrusion of mesial root, palatal inclination, and mesial rotation. Although the initial tooth movement after the application of different force magnitudes until day 3 was not remarkably different, 10 g of force produced more tooth movement compared with heavier forces at day 28.

Download Full-text

Micro-CT data of early physiological cancellous bone formation in the lumbar spine of female C57BL/6 mice

Scientific Data ◽

10.1038/s41597-021-00913-y ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Michael Zenzes ◽

Paul Zaslansky

Keyword(s):

Cancellous Bone ◽

Structural Changes ◽

Bone Growth ◽

Post Partum ◽

Three Dimensional ◽

Lumbar Vertebra ◽

Tissue Growth ◽

Micro Ct ◽

Mineral Density ◽

Ct Data

AbstractMicro-CT provides critical data for musculoskeletal research, yielding three-dimensional datasets containing distributions of mineral density. Using high-resolution scans, we quantified changes in the fine architecture of bone in the spine of young mice. This data is made available as a reference to physiological cancellous bone growth. The scans (n = 19) depict the extensive structural changes typical for female C57BL/6 mice pups, aged 1-, 3-, 7-, 10- and 14-days post-partum, as they attain the mature geometry. We reveal the micro-morphology down to individual trabeculae in the spine that follow phases of mineral-tissue rearrangement in the growing lumbar vertebra on a micrometer length scale. Phantom data is provided to facilitate mineral density calibration. Conventional histomorphometry matched with our micro-CT data on selected samples confirms the validity and accuracy of our 3D scans. The data may thus serve as a reference for modeling normal bone growth and can be used to benchmark other experiments assessing the effects of biomaterials, tissue growth, healing, and regeneration.

Download Full-text

Cartilage: A new parameter for the determination of the postmortem interval?

Journal of Forensic and Legal Medicine ◽

10.1016/j.jflm.2014.08.005 ◽

2014 ◽

Vol 27 ◽

pp. 39-45 ◽

Cited By ~ 10

Author(s):

Armin Alibegović

Keyword(s):

Postmortem Interval

Download Full-text

Erythrocyte Antioxidative Enzymes in Experimentally Induced Osteopenia in Rats

Bulletin of the Veterinary Institute in Pulawy ◽

10.2478/v10213-012-0118-x ◽

2012 ◽

Vol 56 (4) ◽

pp. 669-675 ◽

Cited By ~ 1

Author(s):

Radosław P. Radzki ◽

Marek Bieńko ◽

Edyta Albera ◽

Marta Kankofer

Keyword(s):

Mineral Content ◽

Mineral Density ◽

Initial Body Weight ◽

Dynamic Changes ◽

Bone Parameters ◽

Dynamic Relationship ◽

Healthy Female ◽

Female Wistar Rats ◽

Determination Of Activity

Abstract The study aimed at the determination of dynamic relationship between mineralisation processes and antioxidative/oxidative status during the development of osteopenia. One hundred and two healthy female Wistar rats at the age of 2 months and initial body weight of 200 g were used in the experiment. The rats were divided into control (CON, n=6), sham operated (SHO, n=48), and ovariectomised (OVX, n=48) groups. Animals from SHO (n=6) and OVX (n=6) groups were sacrificed every week during 8 weeks of the experiment in order to detect dynamic changes in examined parameters. The samples were collected weekly from day 7 to day 56. The femora were examined with the use of DXA (bone mineral density) and pQCT (area, mineral content, volumetric density of trabecular and cortical part of distal femora). The pQCT scans were performed 5 mm from distal end of the tibia. The determination of activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in haemolysates of erythrocytes were performed spectrophotometrically. Obtained data showed wave like changes in both enzyme activities and bone parameters and indicated the importance of the 2nd-3rd and 5th-6th week after surgery as a key moment for bone metabolism and activity of enzymatic antioxidative defence during the development of osteopenia induced by bilateral ovariectomy. The obtained results proved that alterations in activity of GSH-Px and SOD, and pQCT ahead the changes registered by DXA by 7 d.

Download Full-text