Plasma homocysteine, serum folic acid, serum vitamin B12, serum vitamin B6, MTHFR and risk of pseudoexfoliation glaucoma: a meta-analysis

Purpose The aim of this study was to assess the association of macular edema (ME) with plasma homocysteine, vitamin B6, vitamin B12, and folic acid levels in patients with Type 2 diabetes. Methods Sixty-five diabetic subjects with no retinopathy and nonproliferative diabetic retinopathy (NPDR) (no DR, without ME, with ME: 16, 25, 24, respectively), 28 with proliferative diabetic retinopathy (PDR) (with and without ME: 14, 14, respectively), and 19 healthy subjects as control were recruited in this cross-sectional study Plasma homocysteine, vitamin B12, vitamin B6, and folate levels were determined after 8-hour of fasting for all subjects. The levels of serum homocysteine and vitamin B6 were measured using high performance liquid chromatography (HPLC) with fluorescence detection, and the levels of serum vitamin B12 and folic acid were measured by electrochemiluminescence immunoassay. Results When diabetic groups with ME were compared with diabetic groups without ME for homocysteine, vitamin B12, vitamin B6, and folic acid, the only significant difference was detected in homocysteine levels (p=0.001). There was no significant difference between NPDR with ME group compared with NPDR without ME group and no DR group for plasma homocysteine, vitamin B12, vitamin B6, and folic acid (p=0.200, p=0.660; p=0.999, p=0.678; p=1.0, p=0.248; p=1.0, p=0.982, respectively). On the other hand, when PDR with ME group was compared with PDR without ME group, there was only significant difference in homocysteine levels (p=0.023). Conclusions Mild to moderate elevation of homocysteine may explain the role of vascular dysregulation and endothelial dysfunction in patients with DR. The present study suggests hyperhomocysteinemia may be one of the crucial risk factors for development of ME.

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Plasma Homocysteine, Folic Acid, and Vitamin B12 Levels in Patients With Pseudoexfoliation Syndrome, Pseudoexfoliation Glaucoma, and Normotensive Glaucoma

Medicina ◽

10.3390/medicina49050034 ◽

2013 ◽

Vol 49 (5) ◽

pp. 34 ◽

Cited By ~ 2

Author(s):

Fatih Türkcü ◽

Özlem Köz ◽

Alper Yarangümeli ◽

Veysi Öner ◽

Gülcan Kural

Keyword(s):

High Performance Liquid Chromatography ◽

Folic Acid ◽

Vitamin B12 ◽

High Performance ◽

Low Cost ◽

Plasma Homocysteine ◽

Pseudoexfoliation Syndrome ◽

Control Group ◽

Healthy Controls ◽

Pseudoexfoliation Glaucoma

Objective. The aim of this study was to evaluate the levels of plasma homocysteine (Hcy), vitamin B12, and folic acid in patients with pseudoexfoliation glaucoma (PEXG), pseudoexfoliation syndrome (PEXS), PEXS plus normotensive glaucoma (NTG).Material and Methods. In total, 24 patients with PEXG, 35 patients with PEXS, 18 patients with PEXS plus NTG, and 35 control subjects were enrolled into study. Their Hcy levels were measured by high performance liquid chromatography (HPLC); the levels of vitamin B12 and folic acid were measured by a competitive electrochemiluminescence immunoassay.Results. Higher plasma Hcy levels and lower folic acid and vitamin B12 levels were found in all 3 patients’ groups compared with the control group (all P<0.001, expect for folic acid in the PEXG group, P=0.03). Although plasma Hcy levels in the PEXG and PEXS groups were similar, the PEXS plus NTG group had significantly higher Hcy levels compared with these groups (P=0.019 and P=0.032, respectively).Conclusions. Our study showed that there was an association between hyperhomocysteinemia and PEXS either with or without glaucoma. The patients with PEXS plus NTG had higher plasma Hcy levels than the patients with PEXS or PEXG and the healthy controls. The treatment of hyperhomocysteinemia by taking low-cost vitamin B12 and folic acid preparations may prevent additional vascular problems.

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Serum Vitamin B12 and Folic Acid Levels in Hyperthyroidism

Nuklearmedizin ◽

10.1055/s-0037-1620915 ◽

1979 ◽

Vol 18 (06) ◽

pp. 278-282 ◽

Cited By ~ 2

Author(s):

Matina Kesse-Elias ◽

D. Koutras ◽

P. Pandos ◽

S. Papazoglou ◽

S. Moulopoulos ◽

...

Keyword(s):

Folic Acid ◽

Vitamin B12 ◽

Serum Vitamin ◽

Mean Value ◽

Clinical Index ◽

The Mean ◽

The Difference ◽

Serum Folic Acid ◽

Hyperthyroid Patients ◽

Normal Controls

SummarySerum vitamin B12 and folic acid levels were measured in 48 hyperthyroid patients and in a group of euthyroid controls. The levels of vitamin B12 ranged from 120-900 pg/ml with a mean of 429.3 ± 30.9 pg/ml (SE). The mean serum vitamin B ? level was lower in hyperthyroid patients than in normal controls, the difference being statistically significant (t = 2.584, p < 0.025). Serum vitamin B12 levels showed a statistically significant negative correlation with the clinical index of Grooks et al. (r = 0.344, p < 0.05). The findings, although not excluding the involvement of auto-immune gastritis in patients with low serum vitamin B12 levels, suggest a direct action of increased thyroid hormone concentrations. Serum folic acid levels ranged from 0.5- 13.8 ng/ml with a mean of 6.8 ± 0.46 ng/ml (SE). The mean serum folic acid levels were higher in the hyperthyroid patients than in normal controls but the difference was not statistically significant (t = 1.2, p > 0.2). The serum folic acid levels did not show any statistically significant correlation with the clinical index of Grooks et al. The fact that no statistically significant difference was found between the mean value in hyperthyroid patients and the mean value in normal controls is probably due to the high folic acid intake in Greece.

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