scholarly journals No secret hiding place? Absence of SARS-CoV-2 on the ocular surface of 1145 hospitalized patients in a pandemic area

2021 ◽  
Author(s):  
Alexander C. Rokohl ◽  
Rafael S. Grajewski ◽  
Philomena A. Wawer Matos ◽  
Hannah-Leah Koch ◽  
Felix Dewald ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Ocular Surface ◽  
Medical Staff ◽  
Care Center ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Eye Care ◽  
Chain Reaction ◽  
Asymptomatic Patients ◽  
Polymerase Chain

Abstract Objectives The aims of this study were to evaluate the isolated prevalence of real-time reverse transcriptase-polymerase chain reaction (RT-PCR)–confirmed SARS-CoV-2 on the ocular surface without systemic infection in hospitalized asymptomatic patients and to determine the risk for ophthalmologists and medical staff to be infected by prescreened asymptomatic patients in a tertiary eye care center. Methods In this prospective, observational study, bilateral swaps of the conjunctiva in the lower fornices as well as nasopharyngeal swaps were collected in 1145 hospitalized asymptomatic patients of a tertiary eye care center. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was performed for each swap to evaluate the prevalence of SARS-CoV-2. Demographic data and potential risk factors for an isolated infection of the ocular surface were noted. Results Two thousand two hundred eighty-eight (99.9%) of all 2290 tested eyes had negative results in the RT-PCR analysis of the conjunctival swabs. One patient had bilateral false-positive results in the conjunctival swabs. None of the 1145 patients had any positive RT-PCR-confirmed result in the nasopharyngeal swabs. Conclusions The risk for an isolated conjunctival viral activity in patients with a negative nasopharyngeal swab-based RT-PCR seems to be absent or extremely low, suggesting no need to perform additional conjunctival swabs in patients with negative nasopharyngeal swabs. Furthermore, the risk of a work-related SARS-CoV-2 infection due to direct contact with preselected asymptomatic patients in an eye care center is very low, especially when additional hygiene standards and safe distances are respected carefully. This might reassure medical staff and reduce the fear of SARS-CoV-2 infection.

Prospective Multi-Institutional Study of Reverse Transcriptase Polymerase Chain Reaction for Molecular Staging of Melanoma

2006 ◽  
Vol 24 (18) ◽  
pp. 2849-2857 ◽  
Author(s):  
Charles R. Scoggins ◽  
Merrick I. Ross ◽  
Douglas S. Reintgen ◽  
R. Dirk Noyes ◽  
James S. Goydos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Peripheral Blood ◽  
Prognostic Significance ◽  
Disease Free Survival ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Molecular Staging ◽  
Polymerase Chain

Purpose To evaluate the prognostic significance of molecular staging using reverse transcriptase polymerase chain reaction (RT-PCR) in detecting occult melanoma cells in sentinel lymph nodes (SLNs) and circulating bloodstream. Patients and Methods In this multicenter study, eligibility criteria included patient age 18 to 71 years, invasive melanoma ≥ 1.0 mm Breslow thickness, and no clinical evidence of metastasis. SLN biopsy and wide excision of the primary tumor were performed. SLNs were examined by serial-section histopathology and S-100 immunohistochemistry. A portion of each SLN was frozen for RT-PCR. In addition, RT-PCR was performed on peripheral-blood mononuclear cells (PBMCs). RT-PCR analysis was performed using four markers: tyrosinase, MART1, MAGE3, and GP-100. Disease-free survival (DFS), distant–DFS (DDFS), and overall survival (OS) were analyzed. Results A total of 1,446 patients with histologically negative SLNs underwent RT-PCR analysis. At a median follow-up of 30 months, there was no difference in DFS, DDFS, or OS between the RT-PCR–positive (n = 620) and RT-PCR–negative (n = 826) patients. Analysis of PBMC from 820 patients revealed significant differences in DFS and DDFS, but not OS, for patients with detection of more than one RT-PCR marker in peripheral blood. Conclusion In this large, prospective, multi-institutional study, RT-PCR analysis on SLNs and PBMCs provides no additional prognostic information beyond standard histopathologic analysis of SLNs. Detection of more than one marker in PBMC is associated with a worse prognosis. RT-PCR remains investigational and should not be used to direct adjuvant therapy at this time.

scholarly journals REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR DNA PIG CONTAMINATION IDENTIFICATION SAUSAGE IN SIX DISTRICT PANDEGLANG BANTEN

2021 ◽  
Vol 1 (1) ◽  
pp. 81-88
Author(s):  
Hadi Susilo
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Meat Product ◽  
Meat Products ◽  
Pcr Analysis ◽  
Processed Meat ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Dna Purity ◽  
Polymerase Chain

Sausage is a meat product processed that is popular food especially in Pandeglang, Banten Province. The importance of halal certificates or the existence of the MUI (Indonesian Ulama Council) halal logo for processed meat products makes Muslim people confident to consume them. The aim this research was to identify pig DNA contamination in sausage products in six  districts in Pandeglang without the MUI halal labels using RT-PCR (Real Time-Polymerase Chain Reaction). RT PCR that can calculate to pig to fill these sample free from pig contamination. This research was divided into two stage, the first stage is extracted or carried out DNA and the second stage is RT PCR analysis. The results of the DNA purity test on sausage samples had DNA purity values ​​of 1.84-1.9 (A260 / A280) and resulted in sample concentrations ranging from 37.8 to 102.5 ng / µl.  The only amplification on the FAM curve was in the positive control pig.  the Cq value ranges from 30 - 31.29. The results of RT PCR on sausage samples in the district area in Pandeglang Banten did not detect the presence of pig DNA.

scholarly journals Real-Time Polymerase chain reaction trends in COVID-19 patients

2020 ◽  
Vol 37 (1) ◽  
Author(s):  
Dr Sana Abbas ◽  
Aisha Rafique ◽  
Dr Beenish Abbas ◽  
Dr Rashid Iqbal
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Tertiary Care ◽  
False Negative ◽  
Polymerase Chain Reaction Test ◽  
Upper Respiratory Tract ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Asymptomatic Patients ◽  
Polymerase Chain

Objective: To assess trends of real-time Polymerase Chain Reaction test in Coronavirus infected Patients. Methods: This cross-sectional analytical study was conducted at Tertiary Care Institute, Rawalpindi from March 2020 to June 2020. All patients confirmed COVID positive by real-time Polymerase Chain Reaction (PCR) with recent travel history, close contact with known diagnosed patients and had symptoms of fever or upper respiratory tract with body aches. Nasopharyngeal swabs were taken and results generated within 48 hours. Positive PCR was admission criteria follow up was carried out at 7th and 8th day, with negative PCR were discharged. However, those who had persistent positive PCR on the 8th day were tested again on 11th and 12th day. Those with persistent positive results beyond 12th day were shifted to specialized quarantine centres. Results: A total of three hundred and ninety-two patients with mild to moderate illness, PCR positive for COVID 19 were included study with age range 9 - 45 and mean 33.22±7.98 years. A total of 8 (2%) patients were females and 384(98%) males. The duration of the negative test result was Mean ± Std. Deviation 9.05±2.00 with 7 – 8 days 152(38.8%)in and 11 – 12 days in 160(40.8%). PCR results on Day 7 and 8 were negative in 144(36.7%) patients whereas positive in 248(63.3%). PCR results on Day 11 and 12 were negative in 312(79.6%) patients whereas positive in 80 (20.4%). Conclusion: To conclude Real-Time Polymerase Chain Reaction (rT-PCR) inclines to give false negative results additionally can stay positive in asymptomatic patients for moderately longer-term. Hence decision to discharge ought to be intricately adjusted between RT-PCR, clinical judgement, radiological examinations, and biochemical assays. doi: https://doi.org/10.12669/pjms.37.1.3000 How to cite this:Abbas S, Rafique A, Abbas B, Iqbal R. Real-Time Polymerase chain reaction trends in COVID-19 patients. Pak J Med Sci. 2021;37(1):180-184. doi: https://doi.org/10.12669/pjms.37.1.3000 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals Assessment of Coronavirus in the Conjunctival Tears in Pediatric Patients with Asymptomatic COVID-19 Infection in Sohag Government, Egypt

2021 ◽  
Vol 15 (1) ◽  
pp. 83-88
Author(s):  
Hany Mahmoud ◽  
Ahmed Radwan ◽  
Amr El Rashidy ◽  
Hesham M. Hefny ◽  
Amal H. Ali ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pediatric Patients ◽  
Nasopharyngeal Swab ◽  
Rt Pcr ◽  
Interventional Study ◽  
Chain Reaction ◽  
Intimate Contact ◽  
Asymptomatic Patients ◽  
Systemic Manifestations ◽  
Polymerase Chain

Objective: The present study aims to evaluate coronavirus shedding in the tears of asymptomatic pediatric COVID-19 positive patients. Methods: A prospective interventional study that included a total of 145 pediatric asymptomatic COVID-19 patients hospitalized from 17th May 2020 to 16th July 2020 in Sohag Tropical Hospital. On admission, all of them were COVID-19 positive detected through nasopharyngeal swab. They were in intimate contact with positive symptomatic COVID-19 patients before testing and admission. Reverse Transcriptase Polymerase chain reaction (RT-PCR) was done for tears samples at an interval of 5 days after admission and twice before discharge. Results: Of the 145 asymptomatic pediatric COVID-19 positive patients, no one showed ocular or systemic manifestations. They were silent carriers. Ten were positive for tears sample on admission. They became negative for nasopharyngeal and tear samples before discharge. Conclusion: Pediatric positive COVID-19 patients can shed coronavirus through their tears. Even among asymptomatic patients, transmission through tears is possible.

scholarly journals STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

2018 ◽  
Vol 13 (1) ◽  
pp. 85 ◽  
Author(s):  
Isti Koesharyani ◽  
Lila Gardenia ◽  
Zakiyah Widowati ◽  
Khumaira Khumaira ◽  
Dita Rustianti
Keyword(s):  
Polymerase Chain Reaction ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Rna Virus ◽  
Pcr Analysis ◽  
Sequencing Analysis ◽  
Accession Number ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Ikan nila atau Oreochromis niloticus merupakan ikan konsumsi masyarakat di Indonesia. Kasus kematian massal ikan nila terjadi di beberapa lokasi budaya di Jawa, Lombok, dan Sumatera yang disebabkan oleh infeksi Orthomyxovirus, dan disebut sebagai Tilapia Lake Virus (TiLV). Tujuan penelitian ini adalah untuk mendeteksi adanya infeksi TiLV dengan metode semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) pada kasus kematian massal ikan nila. Lokasi pengambilan sampel di Desa Sigerongan Kecamatan Lingsar, Lombok, Nusa Tenggara Barat. Analisis deteksi RT-PCR menggunakan sampel organ otak, ginjal, limpa, dan hati, selanjutnya dilakukan sekuensing. Hasil pengamatan terhadap gejala klinis terhadap ikan nila moribund terlihat kondisi mata yang buram/katarak, serta cekung, abrasi kulit, serta perubahan warna tubuh menjadi lebih gelap. Hasil analisis RT-PCR menunjukkan bahwa kejadian kematian massal pada ikan nila suspektif diakibatkan oleh infeksi RNA virus TiLV. Analisis sekuensing menunjukkan bahwa TiLV dari sampel ikan nila di Lombok mempunyai kesamaan identitas genetik 97% dengan TiLV asal Israel (Genebank Accession Number KU 751816.1).Oreochromis niloticus is the main consumption fish commodity in Indonesia. The mortality cases of Nile tilapia have occurred in several culture sites in Java, Lombok, and Sumatra due to the infection of Orthomyxovirus, Tilapia Lake Virus (TiLV). The purpose of this study was to detect the presence of TiLV infection in mass mortality case of Nile tilapia culture using the semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Fish samples were sourced from Segerongan Village Lingsar District, Lombok, West Nusa Tenggara. For RT-PCR analysis, samples from fish brain, kidney, spleen, and liver were collected and treated for sequencing analysis. The visual observation on the moribund tilapia had found several specific clinical symptoms such as eye cataract with sunken eyes, skin abrasion, and darkened body coloration. The result of RT-PCR analysis showed that mass mortalities of tilapia fish had been suspective caused by the infection TiLV RNA virus. The sequencing analysis showed that TiLV samples from Lombok have a genetic similarity of 97% with TiLV from Israeli (Genebank Accession Number KU 751816.1).

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