scholarly journals Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue

2012 ◽  
Vol 137 (6) ◽  
pp. 841-847 ◽  
Author(s):  
Akemi Shimada ◽  
Koichiro Komatsu ◽  
Kazuhisa Nakashima ◽  
Ernst Pöschl ◽  
Akira Nifuji
Keyword(s):  
Hard Tissue ◽  
Lacz Activity ◽  
Improved Methods

Improved methods for immunohistochemical detection of BrdU in hard tissue

2008 ◽  
Vol 339 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Akemi Shimada ◽  
Tatsuya Shibata ◽  
Koichiro Komatsu ◽  
Akira Nifuji
Keyword(s):  
Immunohistochemical Detection ◽  
Hard Tissue ◽  
Improved Methods

Effects of Hypophysectomy on the Occurrence and Calcifiability of Matrix Vesicles in the Epiphyseal Growth Plate of Immature Rats

1980 ◽  
Vol 38 ◽  
pp. 578-579
Author(s):  
S. I. Coleman ◽  
W. J. Dougherty
Keyword(s):  
Growth Plate ◽  
Matrix Vesicles ◽  
Epiphyseal Growth Plate ◽  
Hard Tissue ◽  
Hormonal Factors ◽  
Post Surgery ◽  
Mineral Deposition ◽  
Thin Sectioning ◽  
Integral Role ◽  
Cellular Secretion

In the cellular secretion theory of mineral deposition, extracellular matrix vesicles are believed to play an integral role in hard tissue mineralization (1). Membrane limited matrix vesicles arise from the plasma membrane of epiphyseal chondrocytes and tooth odontoblasts by a budding process (2, 3). Nutritional and hormonal factors have been postulated to play essential roles in mineral deposition and apparently have a direct effect on matrix vesicles of calcifying cartilage as concluded by Anderson and Sajdera (4). Immature (75-85 gm) Long-Evans hooded rats were hypophysectomized by the parapharyngeal approach and maintained fourteen (14) days post-surgery. At this time, the animals were anesthetized and perfusion fixed in cacodylate buffered 2.5% glutaraldehyde. The proximal tibias were quickly dissected out and split sagittally. One half was used for light microscopy (LM) and the other for electron microscopy (EM). The halves used for EM were cut into blocks approximately 1×3 mm. The tissue blocks were prepared for ultra-thin sectioning and transmission EM. The tissue was oriented so as to section through the epiphyseal growth plate from the zone of proliferating cartilage on down through the hypertrophic zone and into the initial trabecular bone. Sections were studied stained (double heavy metal) and unstained.

Improved methods for characterizing material-induced contamination

1983 ◽  
Author(s):  
A. GLASSFORD ◽  
R. OSIECKI ◽  
C.-K. LIU ◽  
M. HITCHCOCK
Keyword(s):  
Improved Methods

Challenges and opportunities for the affective sciences.

2018 ◽  
Author(s):  
Andrew S. Fox ◽  
Regina Lapate ◽  
Alexander J. Shackman ◽  
Richard J Davidson
Keyword(s):  
Well Being ◽  
Human Condition ◽  
Emotional States ◽  
Quarter Century ◽  
Core Feature ◽  
Challenges And Opportunities ◽  
The Mind ◽  
The Impact ◽  
Improved Methods ◽  
Health And Well Being

Emotion is a core feature of the human condition, with profound consequences for health, wealth, and wellbeing. Over the past quarter-century, improved methods for manipulating and measuring different features of emotion have yielded steady advances in our scientific understanding emotional states, traits, and disorders. Yet, it is clear that most of the work remains undone. Here, we highlight key challenges facing the field of affective sciences. Addressing these challenges will provide critical opportunities not just for understanding the mind, but also for increasing the impact of the affective sciences on public health and well-being.

Results of a comparative study of the use of fodder worms of two breeds for pool growing of young tilapy and clary catfish in the conditions of the Almaty

2020 ◽  
pp. 73-80
Author(s):  
Z. Bolatbekova ◽  
S. Assylbekova ◽  
B. Kulatayev ◽  
S. Koishybayeva ◽  
N. Bulavina
Keyword(s):  
Comparative Study ◽  
Biochemical Composition ◽  
Nutritional Value ◽  
Fish Farming ◽  
Biological Indicators ◽  
Comprehensive Analysis ◽  
Fish Feed ◽  
Fish Breeding ◽  
Breeding Zone ◽  
Improved Methods

The aryicle presents the results of a comparative study of the biochemical composition of earthworms of dendrobene and prospector rocks, the peculiarities of their cultivation and their use for feeding when growing juvenile tilapia and clary catfish. The experience of cultivating earthworms of two breeds using the improved methods of foreign authors is described. The results of a study of the biochemical composition of cultivated objects that determine their nutritional value for feeding fish are presented. The fish-breeding and biological indicators of juvenile tilapia and clary catfish, obtained during the cultivation of dendrobene and prospector in fish farming in the Almaty region (VI fish-breeding zone) during worm feeding, are analyzed. A comprehensive analysis of the studied indicators made it possible to assign a certain rating place to each worm breed in relation to its use as fish feed.

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