Vermamoeba vermiformis as etiological agent of a painful ulcer close to the eye

AbstractSerotyping is the most common method to characterize field isolates of Actinobacillus (A.) pleuropneumoniae, the etiological agent of porcine pleuropneumonia. Based on serology, many farms seem to be infected and antibodies against a wide variety of serovars are detectable, but, so far it is unknown to what degree respective serovars contribute to outbreaks of clinical manifest disease. In this study, 213 German A. pleuropneumoniae field isolates retrieved for diagnostic purposes from outbreaks of porcine pleuropneumonia between 2010 and 2019 were genetically serotyped and analyzed regarding their apx-toxin gene profile using molecular methods. Serotyping revealed a prominent role of serovar 2 in clinical cases (64% of all isolates) and an increase in the detection of this serovar since 2010 in German isolates. Serovar 9/11 followed as the second most frequent serovar with about 15% of the isolates. Furthermore, very recently described serovars 16 (n = 2) and 18 (n = 8) were detected. Most isolates (93.4%) showed apx-profiles typical for the respective serovar. However, this does not hold true for isolates of serovar 18, as 75% (n = 6) of all isolates of this serovar deviated uniformly from the “typical” apx-gene profile of the reference strain 7311555. Notably, isolates from systemic lesions such as joints or meninges did not harbor the complete apxICABD operon which is considered typical for highly virulent strains. Furthermore, the extremely low occurrence (n = 1) of NAD independent (biovar II) isolates in German A. pleuropneumoniae was evident in our collection of clinical isolates.

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A/H1N1 hemagglutinin antibodies show comparable affinity in vaccine-related Narcolepsy type 1 and control and are unlikely to contribute to pathogenesis

Scientific Reports ◽

10.1038/s41598-021-83543-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Alexander Lind ◽

Ilaria Marzinotto ◽

Cristina Brigatti ◽

Anita Ramelius ◽

Lorenzo Piemonti ◽

...

Keyword(s):

Antibody Response ◽

Antigenic Determinant ◽

Etiological Agent ◽

Healthy Controls ◽

Antibody Affinity ◽

Antibody Titres ◽

Antibody Levels ◽

And Control ◽

Radiobinding Assay

AbstractAn increased incidence of narcolepsy type 1 (NT1) was observed in Scandinavia following the 2009–2010 influenza Pandemrix vaccination. The association between NT1 and HLA-DQB1*06:02:01 supported the view of the vaccine as an etiological agent. A/H1N1 hemagglutinin (HA) is the main antigenic determinant of the host neutralization antibody response. Using two different immunoassays, the Luciferase Immunoprecipitation System (LIPS) and Radiobinding Assay (RBA), we investigated HA antibody levels and affinity in an exploratory and in a confirmatory cohort of Swedish NT1 patients and healthy controls vaccinated with Pandemrix. HA antibodies were increased in NT1 patients compared to controls in the exploratory (LIPS p = 0.0295, RBA p = 0.0369) but not in the confirmatory cohort (LIPS p = 0.55, RBA p = 0.625). HA antibody affinity, assessed by competition with Pandemrix vaccine, was comparable between patients and controls (LIPS: 48 vs. 39 ng/ml, p = 0.81; RBA: 472 vs. 491 ng/ml, p = 0.65). The LIPS assay also detected higher HA antibody titres as associated with HLA-DQB1*06:02:01 (p = 0.02). Our study shows that following Pandemrix vaccination, HA antibodies levels and affinity were comparable NT1 patients and controls and suggests that HA antibodies are unlikely to play a role in NT1 pathogenesis.

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Tularemia as a Mosquito-Borne Disease

Microorganisms ◽

10.3390/microorganisms9010026 ◽

2020 ◽

Vol 9 (1) ◽

pp. 26

Author(s):

Zakaria Abdellahoum ◽

Max Maurin ◽

Idir Bitam

Keyword(s):

North America ◽

Northern Hemisphere ◽

Francisella Tularensis ◽

Research Work ◽

Warm Season ◽

Epidemiological Data ◽

Human Infection ◽

Etiological Agent ◽

Etiologic Agents

Francisella tularensis (Ft) is the etiological agent of tularemia, a disease known for over 100 years in the northern hemisphere. Ft includes four subspecies, of which two are the etiologic agents of tularemia: Ft subsp. tularensis (Ftt) and Ft subsp. holarctica (Fth), mainly distributed in North America and the whole northern hemisphere, respectively. Several routes of human infection with these bacteria exist, notably through bites of Ixodidae ticks. However, mosquitoes represent the main vectors of Fth in Scandinavia, where large tularemia outbreaks have occurred, usually during the warm season. The mechanisms making mosquitoes vectors of Fth are still unclear. This review covers the inventory of research work and epidemiological data linking tularemia to mosquitoes in Scandinavia and highlights the gaps in understanding mosquitoes and Ft interactions.

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Genome Sequence of Aggregatibacter actinomycetemcomitans Serotype c Strain D11S-1

Journal of Bacteriology ◽

10.1128/jb.01203-09 ◽

2009 ◽

Vol 191 (23) ◽

pp. 7378-7379 ◽

Cited By ~ 29

Author(s):

Casey Chen ◽

Weerayuth Kittichotirat ◽

Yan Si ◽

Roger Bumgarner

Keyword(s):

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Aggregatibacter Actinomycetemcomitans ◽

Aggressive Periodontitis ◽

Etiological Agent ◽

Subgingival Plaque

ABSTRACT Aggregatibacter actinomycetemcomitans is a major etiological agent of periodontitis. Here we report the complete genome sequence of serotype c strain D11S-1, which was recovered from the subgingival plaque of a patient diagnosed with generalized aggressive periodontitis.

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Correlation between Detection of a Plasmid and High-Level Virulence of Vibrio nigripulchritudo, a Pathogen of the Shrimp Litopenaeus stylirostris

Applied and Environmental Microbiology ◽

10.1128/aem.02680-07 ◽

2008 ◽

Vol 74 (10) ◽

pp. 3038-3047 ◽

Cited By ~ 14

Author(s):

Yann Reynaud ◽

Denis Saulnier ◽

Didier Mazel ◽

Cyrille Goarant ◽

Frédérique Le Roux

Keyword(s):

Genetic Markers ◽

New Caledonia ◽

Subtractive Hybridization ◽

Epidemiological Studies ◽

Etiological Agent ◽

Subtraction Library ◽

High Virulence ◽

High Level ◽

Mass Mortalities ◽

Coral Pathogen

ABSTRACT Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates from our collection (n = 32), and 13 were detected only in the isolates demonstrating the highest pathogenicity (n = 19), suggesting that they could be used as genetic markers for high virulence capacity. Interestingly, 10 of these markers are carried by a replicon of 11.2 kbp that contains sequences highly similar to those of a plasmid detected in Vibrio shilonii, a coral pathogen. The detection of this plasmid was correlated with the highest pathogenicity status of the isolates from our collection. The origin and consequence of this plasmid acquisition are discussed.

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Antagonistic effect of gut microbiota of the Egyptian honeybees, Apis mellifera L. against the etiological agent of Stonebrood disease

International Journal of Tropical Insect Science ◽

10.1007/s42690-021-00654-w ◽

2021 ◽

Author(s):

Amr M. Shehabeldine ◽

Amr H. Hashem ◽

Ahmed I. Hasaballah

Keyword(s):

Apis Mellifera ◽

Gut Microbiota ◽

Antagonistic Effect ◽

Etiological Agent

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Host-Etiological Agent Interactions in Intranasally and Intraperitoneally Induced Cryptococcosis in Mice

Infection and Immunity ◽

10.1128/iai.29.2.633-641.1980 ◽

1980 ◽

Vol 29 (2) ◽

pp. 633-641 ◽

Cited By ~ 1

Author(s):

Thuang S. Lim ◽

Juneann W. Murphy ◽

Larry K. Cauley

Keyword(s):

Etiological Agent ◽

Delayed Type Hypersensitivity ◽

In Vivo Model ◽

Spleen Cells ◽

Polysaccharide Antigen ◽

Cryptococcal Antigen ◽

Agent Interactions ◽

Growth Profiles ◽

Host Parasite

Inbred CBA/J mice were used in developing a defined in vivo model for studying host-parasite relationships in cryptococcosis. Mice were infected either intranasally or intraperitoneally with 10 3 viable Cryptococcus neoformans cells. At weekly intervals over a 92-day period, C. neoformans growth profiles in the lungs, spleens, livers, and brains of the infected animals were determined. In addition, humoral and delayed-type hypersensitivity responses and cryptococcal antigen levels were assayed in these mice. Intranasally infected mice developed strong delayed-type hypersensitivity reactions in response to cryptococcal culture filtrate (CneF) antigen, and there was good correlation between acquisition of delayed-type hypersensitivity and the reduction of C. neoformans cell numbers in infected tissues. In contrast, intraperitoneally infected mice displayed greater numbers of C. neoformans cells in tissues and had somewhat suppressed delayed-type hypersensitivity responses to CneF antigen. Anticryptococcal antibodies were not detected in intranasally or intraperitoneally infected mice, but cryptococcal polysaccharide antigen titers were relatively high in both groups. The transfer of sensitized spleen cells from intranasally infected mice to syngeneic naive recipient mice resulted in the transfer of delayed-type hypersensitivity responsiveness to cryptococcal antigen in the recipients. The intranasally induced infection in mice was similar to the naturally acquired infection in humans; therefore we are proposing that this murine-cryptococcosis model would be useful in gaining a greater understanding of host-etiological agent relationships in this disease.

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Aetiological Outcome of Mesenteric Lymphadenopathy and Pain Abdomen in Paediatric Population at a Tertiary Care Hospital (MRIMSHyderabad) - A Hospital Based Prospective Observational Study

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/480 ◽

2021 ◽

Vol 8 (29) ◽

pp. 2601-2608

Author(s):

Dileep Kumar Allagadda ◽

Harikrishna Appana ◽

Ramu Pedada ◽

Deepika Gurram ◽

Ditin Joseph

Keyword(s):

Abdominal Pain ◽

Observational Study ◽

Prospective Observational Study ◽

Tertiary Care ◽

Paediatric Population ◽

Etiological Agent ◽

Parasitic Infections ◽

Care Hospital ◽

Age Group ◽

Mesenteric Lymphadenitis

BACKGROUND Abdominal pain is a common disorder in children and adolescents worldwide with prevalence rate ranging from 20 - 25 % in school-going children in India. It is a frustrating concern to the child, parents and the physician. Even though abdominal pain is one of the most common complaints in children, it poses a diagnostic challenge owing to the variety of underlying causes. The purpose of this study was to evaluate the aetiology of abdominal pain associated with significant mesenteric lymphadenopathy in a paediatric population. METHODS This is a hospital based prospective, observational study done in Department of Paediatrics, Malla Reddy Institute of Medical Sciences (MRIMS), Hyderabad. RESULTS Out of the 65 children studied, 30.8 % were of 5 - 8 years age group, 36.9 % were 9 - 12 years age group and 32.3 % were 13 - 15 years age group. In our study, we found 50.8 % were boys and 49.2 % were girls. 20 % of them were having fever, 16.9 % were having cough, 16.9 % were having diarrhoea, 13.8 % were having sore throat, 16.9 % dysuria, and 15.4 % were having constipation. All patients in our study group underwent ultrasound of abdomen. All cases were having significant mesenteric lymphadenopathy (more than 5 mm in short axis with three or more number of lymph nodes). In 53.8 % cases, etiological agent for the mesenteric lymphadenopathy was not proved with our investigations. In the remaining 46.2 % of children, cause of mesenteric lymphadenopathy was proved and 38.5 % were bacterial infections, 6.2 % were viral infections and 1.5 % were parasitic infections. CONCLUSIONS It is important to recognise mesenteric lymphadenitis as a clinical entity in paediatric cases presenting with abdominal pain. They should be evaluated for an etiological agent and if no proven source of infection and etiological agent is found, it can be considered as functional abdominal pain. If we are able to get a proper etiological diagnosis in these cases, we could treat them and we could make huge difference in terms of quality of life. KEYWORDS Abdominal Pain, Mesenteric Lymphadenitis, Ultrasound, Significant Mesenteric Lymphadenopathy

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