Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods

2021 ◽  
Author(s):  
Brenda P. F. A. Gomes ◽  
Juliana D. Bronzato ◽  
Rebecca F. Almeida-Gomes ◽  
Ericka T. Pinheiro ◽  
Ezilmara L. R. Sousa ◽  
...  
Keyword(s):  
Clinical Features ◽  
Fusobacterium Nucleatum ◽  
Endodontic Infections

scholarly journals Filling the Void: An Optimized Polymicrobial Interkingdom Biofilm Model for Assessing Novel Antimicrobial Agents in Endodontic Infection

2020 ◽  
Vol 8 (12) ◽  
pp. 1988
Author(s):  
Sumaya Abusrewil ◽  
Jason L. Brown ◽  
Christopher D. Delaney ◽  
Mark C. Butcher ◽  
Ryan Kean ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Fusobacterium Nucleatum ◽  
Atmospheric Conditions ◽  
Candida Spp ◽  
Biofilm Growth ◽  
Log Reduction ◽  
Biofilm Model ◽  
Endodontic Infection ◽  
Endodontic Infections ◽  
Ontological Analysis

There is a growing realization that endodontic infections are often polymicrobial, and may contain Candida spp. Despite this understanding, the development of new endodontic irrigants and models of pathogenesis remains limited to mono-species biofilm models and is bacterially focused. The purpose of this study was to develop and optimize an interkingdom biofilm model of endodontic infection and use this to test suitable anti-biofilm actives. Biofilms containing Streptococcus gordonii, Fusobacterium nucleatum, Porphyromonas gingivalis, and Candida albicans were established from ontological analysis. Biofilms were optimized in different media and atmospheric conditions, prior to quantification and imaging, and subsequently treated with chlorhexidine, EDTA, and chitosan. These studies demonstrated that either media supplemented with serum were equally optimal for biofilm growth, which were dominated by S. gordonii, followed by C. albicans. Assessment of antimicrobial activity showed significant effectiveness of each antimicrobial, irrespective of serum. Chitosan was most effective (3 log reduction), and preferentially targeted C. albicans in both biofilm treatment and inhibition models. Chitosan was similarly effective at preventing biofilm growth on a dentine substrate. This study has shown that a reproducible and robust complex interkingdom model, which when tested with the antifungal chitosan, supports the notion of C. albicans as a key structural component.

scholarly journals ANTIBACTERIAL EFFECT OF XANTHORRHIZOL (CURCUMA XANTHORRHIZA ROXB.) AGAINST THE BIOFILM OF FUSOBACTERIUM NUCLEATUM

2020 ◽  
pp. 57-61
Author(s):  
MAIDA FITRI ◽  
KAMIZAR NAZAR ◽  
RATNA MEIDYAWATI ◽  
REZA AZMI
Keyword(s):  
Cell Viability ◽  
Mtt Assay ◽  
Antibacterial Effect ◽  
Cell Metabolism ◽  
Fusobacterium Nucleatum ◽  
Common Cause ◽  
Irrigation Solution ◽  
The Common ◽  
Endodontic Infections ◽  
Curcuma Xanthorrhiza

Objective: Endodontic infections are treated with a root canal; one of the stages involves using an irrigation solution to eliminate microorganisms.Sodium hypochlorite (NaOCl) is an irrigation solution used to eliminate Fusobacterium nucleatum, the common cause of primary endodonticinfections. However, this material is synthetic and can cause side effects and inflammation of the periapical tissues. Thus, an irrigation solution, suchas xanthorrhizol, which is made of natural ingredients, is required. However, xanthorrhizol’s antibacterial effect against F. nucleatum has never beenassessed. Thus, this study aimed to analyze the effect of xanthorrhizol, derived from Curcuma xanthorrhiza Roxb., against F. nucleatum ATCC 25586biofilm.Methods: The methylthiazol tetrazolium (MTT) assay test and colony count test were performed to assess F. nucleatum eradication after exposure toxanthorrhizol at various concentrations (0.5%, 0.75%, 1%, 1.25%, and 1.5%) and 2.5% NaOCl, which was used as positive control.Results: Significant differences were observed in terms of cell viability after treatment with xanthorrhizol at concentrations of 1.25% versus 0.5%,0.75%, and 1%. The MTT assay test was used to evaluate cell viability in the biofilm and cell metabolism activities. Results showed no significantdifferences in terms of efficacy between xanthorrhizol at concentrations of 1.25% and 1.5%, which are considered effective against F. nucleatum, and2.5% NaOCl (p>0.05).Conclusion: The effect of xanthorrhizol at concentrations of 1.25% and 1.5% against F. nucleatum is similar to that of 2.5% NaOCl.

Microflora associated with primary endodontic infections: Correlations among sem evaluation, clinical features, and radiographic findings

2012 ◽  
Vol 75 (11) ◽  
pp. 1557-1563 ◽  
Author(s):  
Flávia Emi Razera Baldasso ◽  
Caroline Patta Stürmer ◽  
Simone Bonato Luisi ◽  
Maria Noel Marzano Rodrigues Petruzzi ◽  
Roberta Kochenborger SCARPARO ◽  
...  
Keyword(s):  
Clinical Features ◽  
Radiographic Findings ◽  
Endodontic Infections

Presence of Oral Bacterial Species in Primary Endodontic Infections of Primary Teeth

2013 ◽  
Vol 38 (2) ◽  
pp. 155-160 ◽  
Author(s):  
N Topcuoglu ◽  
E Bozdoğan ◽  
O Aktoren ◽  
G Kulekci
Keyword(s):  
Primary Teeth ◽  
Bacterial Species ◽  
Treatment Strategies ◽  
Fusobacterium Nucleatum ◽  
Primary Tooth ◽  
Microarray Technology ◽  
Microbial Composition ◽  
Root Canals ◽  
Endodontic Infections ◽  
Clinical Conditions

Objective: Knowledge of the microbial composition of deciduous endodontic infections is limited. This study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. Study Design: The samples were collected from 30 root canals of primary teeth with primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (Parocheck®). Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequently isolated bacterium (96.7%), followed by Prevotella intermedia (86.7%), Parvimonas micra (83.3%), Treponema denticola (76.7%) and Tannerella forsythia (66.7%). These bacteria were also present in high levels. All pairs of bacterial species were positively associated (RR≯1), except P.intermedia and P.micra. On average, five species (range:3-8) were detected per amplified sample. Root canals of teeth with ≯5 different species were statistically associated with periapical radiolucency (P=0.049). Conclusions: Primary teeth with endodontic infections show a highly diverse variety of bacteria, in which the most prevalent specie are present in high proportions. The well-directed use of the improved microarray technology will provide additional valuable information for causative factors associated with endodontic diseases, helping to develop more successful antibacterial or anti-inflammatory treatment strategies.

scholarly journals Pathogenic Bacterial Species Associated with Endodontic Infection Evade Innate Immune Control by Disabling Neutrophils

2014 ◽  
Vol 82 (10) ◽  
pp. 4068-4079 ◽  
Author(s):  
Aritsune Matsui ◽  
Jun-O Jin ◽  
Christopher D. Johnston ◽  
Hajime Yamazaki ◽  
Yael Houri-Haddad ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Oral Bacteria ◽  
Innate Immune ◽  
Tooth Pulp ◽  
Content Type ◽  
Endodontic Infection ◽  
Parvimonas Micra ◽  
Endodontic Infections

ABSTRACTEndodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. To investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections (endodontic pathogens [EP]), i.e.,Fusobacterium nucleatum,Streptococcus intermedius,Parvimonas micra, andPrevotella intermedia, was inoculated into subcutaneously implanted titanium chambers. Cells that infiltrated the chamber after these infections were primarily neutrophils; however, these neutrophils were unable to control the infection. Infection with a nonpathogenic oral bacterial species,Streptococcus mitis, resulted in well-controlled infection, with bacterial numbers reduced by 4 to 5 log units after 7 days. Propidium iodide (PI) staining of the chamber neutrophils identified three distinct populations: neutrophils from EP-infected chambers were intermediate in PI staining, while cells in chambers from mice infected withS. mitiswere PI positive (apoptotic) or negative (live). Strikingly, neutrophils from EP-infected chambers were severely impaired in their ability to phagocytose and to generate reactive oxygen speciesin vitroafter removal from the chamber compared to cells fromS. mitis-infected chambers. The mechanism of neutrophil impairment was necrotic cell death as determined by morphological analyses.P. intermediaalone could induce a similar neutrophil phenotype. We conclude that the endodontic pathogens, particularlyP. intermedia, can efficiently disable and kill infiltrating neutrophils, allowing these infections to become established. These results can help explain the persistence of endodontic infections and demonstrate a new virulence mechanism associated withP. intermedia.

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