scholarly journals Transcriptomic analysis of bacteriocin synthesis and stress response in Lactobacillus paracasei HD1.7 under acetic acid stress

LWT ◽  
2021 ◽  
pp. 112897
Author(s):  
Jie Kang ◽  
Xiaohang Zhou ◽  
Wen Zhang ◽  
Fangyi Pei ◽  
Jingping Ge
Keyword(s):  
Acetic Acid ◽  
Stress Response ◽  
Acid Stress ◽  
Transcriptomic Analysis ◽  
Lactobacillus Paracasei

Transcriptomic analysis of formic acid stress response in Saccharomyces cerevisiae

2022 ◽  
Vol 38 (2) ◽  
Author(s):  
Lingjie Zeng ◽  
Jinxiang Huang ◽  
Pixue Feng ◽  
Xuemei Zhao ◽  
Zaiyong Si ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Formic Acid ◽  
Acid Stress ◽  
Transcriptomic Analysis ◽  
Acid Stress Response

scholarly journals Sphingolipid biosynthesis upregulation by TOR complex 2–Ypk1 signaling during yeast adaptive response to acetic acid stress

2016 ◽  
Vol 473 (23) ◽  
pp. 4311-4325 ◽  
Author(s):  
Joana F. Guerreiro ◽  
Alexander Muir ◽  
Subramaniam Ramachandran ◽  
Jeremy Thorner ◽  
Isabel Sá-Correia
Keyword(s):  
Acetic Acid ◽  
Protein Kinase ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Acid Stress ◽  
Acid Tolerance ◽  
Industrial Biotechnology ◽  
Acetic Acid Tolerance ◽  
Spoilage Yeasts ◽  
Sphingolipid Biosynthesis

Acetic acid-induced inhibition of yeast growth and metabolism limits the productivity of industrial fermentation processes, especially when lignocellulosic hydrolysates are used as feedstock in industrial biotechnology. Tolerance to acetic acid of food spoilage yeasts is also a problem in the preservation of acidic foods and beverages. Thus understanding the molecular mechanisms underlying adaptation and tolerance to acetic acid stress is increasingly important in industrial biotechnology and the food industry. Prior genetic screens for Saccharomyces cerevisiae mutants with increased sensitivity to acetic acid identified loss-of-function mutations in the YPK1 gene, which encodes a protein kinase activated by the target of rapamycin (TOR) complex 2 (TORC2). We show in the present study by several independent criteria that TORC2–Ypk1 signaling is stimulated in response to acetic acid stress. Moreover, we demonstrate that TORC2-mediated Ypk1 phosphorylation and activation is necessary for acetic acid tolerance, and occurs independently of Hrk1, a protein kinase previously implicated in the cellular response to acetic acid. In addition, we show that TORC2–Ypk1-mediated activation of l-serine:palmitoyl-CoA acyltransferase, the enzyme complex that catalyzes the first committed step of sphingolipid biosynthesis, is required for acetic acid tolerance. Furthermore, analysis of the sphingolipid pathway using inhibitors and mutants indicates that it is production of certain complex sphingolipids that contributes to conferring acetic acid tolerance. Consistent with that conclusion, promoting sphingolipid synthesis by adding exogenous long-chain base precursor phytosphingosine to the growth medium enhanced acetic acid tolerance. Thus appropriate modulation of the TORC2–Ypk1–sphingolipid axis in industrial yeast strains may have utility in improving fermentations of acetic acid-containing feedstocks.

Acid stress response in environmental and clinical strains of enteric bacteria

2012 ◽  
Vol 7 (6) ◽  
pp. 495-505 ◽  
Author(s):  
Gabriel J. Swenson ◽  
J. Stochastic ◽  
Franklyn F. Bolander ◽  
Richard A. Long
Keyword(s):  
Stress Response ◽  
Acid Stress ◽  
Enteric Bacteria ◽  
Clinical Strains ◽  
Acid Stress Response

Different response to acetic acid stress inSaccharomyces cerevisiaewild-type andl-ascorbic acid-producing strains

Yeast ◽  
2013 ◽  
Vol 30 (9) ◽  
pp. 365-378 ◽  
Author(s):  
Francesca Martani ◽  
Tiziana Fossati ◽  
Riccardo Posteri ◽  
Lorenzo Signori ◽  
Danilo Porro ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Acetic Acid ◽  
Acid Stress ◽  
Different Response

scholarly journals Modulation of Responses of Vibrio parahaemolyticus O3:K6 to pH and Temperature Stresses by Growth at Different Salt Concentrations

2010 ◽  
Vol 76 (14) ◽  
pp. 4720-4729 ◽  
Author(s):  
W. Brian Whitaker ◽  
Michelle A. Parent ◽  
Lynn M. Naughton ◽  
Gary P. Richards ◽  
Seth L. Blumerman ◽  
...  
Keyword(s):  
Stress Response ◽  
Vibrio Parahaemolyticus ◽  
Acid Stress ◽  
The United States ◽  
Stress Conditions ◽  
Moderate Halophile ◽  
Growth And Survival ◽  
Absolute Requirement ◽  
Acid Acid ◽  
Bacterial Gastroenteritis

ABSTRACT Vibrio parahaemolyticus inhabits marine, brackish, and estuarine waters worldwide, where fluctuations in salinity pose a constant challenge to the osmotic stress response of the organism. Vibrio parahaemolyticus is a moderate halophile, having an absolute requirement for salt for survival, and is capable of growth at 1 to 9% NaCl. It is the leading cause of seafood-related bacterial gastroenteritis in the United States and much of Asia. We determined whether growth in differing NaCl concentrations alters the susceptibility of V. parahaemolyticus O3:K6 to other environmental stresses. Vibrio parahaemolyticus was grown at a 1% or 3% NaCl concentration, and the growth and survival of the organism were examined under acid or temperature stress conditions. Growth of V. parahaemolyticus in 3% NaCl versus that in 1% NaCl increased survival under both inorganic (HCl) and organic (acetic acid) acid conditions. In addition, at 42°C and −20°C, 1% NaCl had a detrimental effect on growth. The expression of lysine decarboxylase (encoded by cadA), the organism's main acid stress response system, was induced by both NaCl and acid conditions. To begin to address the mechanism of regulation of the stress response, we constructed a knockout mutation in rpoS, which encodes the alternative stress sigma factor, and in toxRS, a two-component regulator common to many Vibrio species. Both mutant strains had significantly reduced survival under acid stress conditions. The effect of V. parahaemolyticus growth in 1% or 3% NaCl was examined using a cytotoxicity assay, and we found that V. parahaemolyticus grown in 1% NaCl was significantly more toxic than that grown in 3% NaCl.

scholarly journals Endogenous lycopene improves ethanol production under acetic acid stress in Saccharomyces cerevisiae

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Shuo Pan ◽  
Bin Jia ◽  
Hong Liu ◽  
Zhen Wang ◽  
Meng-Zhe Chai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Ethanol Production ◽  
Acid Stress

Integrative multiomics analysis of the acid stress response of Oenococcus oeni mutants at different growth stages

2021 ◽  
pp. 103905
Author(s):  
Qiling Chen ◽  
Xiangke Yang ◽  
Qiang Meng ◽  
Lili Zhao ◽  
Yuxin Yuan ◽  
...  
Keyword(s):  
Stress Response ◽  
Acid Stress ◽  
Oenococcus Oeni ◽  
Growth Stages ◽  
Acid Stress Response ◽  
Different Growth Stages

Identification of IAA-regulated genes in Pseudomonas syringae pv. tomato strain DC3000

2021 ◽  
Author(s):  
Arnaud-Thierry Djami-Tchatchou ◽  
Zipeng Alex Li ◽  
Paul Stodghill ◽  
Melanie J. Filiatrault ◽  
Barbara N. Kunkel
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Stress Response ◽  
Pseudomonas Syringae ◽  
Plant Pathogen ◽  
Type Iii Secretion ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid ◽  
Exogenous Iaa

The auxin indole-3-acetic acid (IAA) is a plant hormone that not only regulates plant growth and development but also plays important roles in plant-microbe interactions. We previously reported that IAA alters expression of several virulence-related genes in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000 ( Pto DC3000). To learn more about the impact of IAA on regulation of Pto DC3000 gene expression we performed a global transcriptomic analysis of bacteria grown in culture, in the presence or absence of exogenous IAA. We observed that IAA repressed expression of genes involved in the Type III secretion (T3S) system and motility and promoted expression of several known and putative transcriptional regulators. Several of these regulators are orthologs of factors known to regulate stress responses and accordingly expression of several stress response-related genes was also upregulated by IAA. Similar trends in expression for several genes were also observed by RT-qPCR. Using an Arabidopsis thaliana auxin receptor mutant that accumulates elevated auxin, we found that many of the P. syringae genes regulated by IAA in vitro were also regulated by auxin in planta . Collectively the data indicate that IAA modulates many aspects of Pto DC3000 biology, presumably to promote both virulence and survival under stressful conditions, including those encountered in or on plant leaves. IMPORTANCE Indole-3-acetic acid (IAA), a form of the plant hormone auxin, is used by many plant-associated bacteria as a cue to sense the plant environment. Previously, we showed that IAA can promote disease in interactions between the plant pathogen Pseudomonas syringae strain Pto DC000 and one of its hosts, Arabidopsis thaliana . However, the mechanisms by which IAA impacts the biology of Pto DC3000 and promotes disease are not well understood. Here we demonstrate that IAA is a signal molecule that regulates gene expression in Pto DC3000. The presence of exogenous IAA affects expression of over 700 genes in the bacteria, including genes involved in Type III secretion and genes involved in stress response. This work offers insight into the roles of auxin promoting pathogenesis.

scholarly journals Differential View on the Bile Acid Stress Response of Clostridioides difficile

2019 ◽  
Vol 10 ◽  
Author(s):  
Susanne Sievers ◽  
Nicole G. Metzendorf ◽  
Silvia Dittmann ◽  
Daniel Troitzsch ◽  
Viola Gast ◽  
...  
Keyword(s):  
Bile Acid ◽  
Stress Response ◽  
Acid Stress ◽  
Clostridioides Difficile ◽  
Acid Stress Response
Sign in / Sign up

Export Citation Format

Share Document