Optimized thermochemical detoxification of asbestos-containing waste using chemical additives and microwave heat treatment

Author(s):  
Myung Hwan Hong ◽  
So Young Joo ◽  
Dong Wook Kim ◽  
Jin-Ho Yoon
2001 ◽  
Vol 7 (5) ◽  
pp. 405-409 ◽  
Author(s):  
R. Kirby ◽  
M. da Silva ◽  
C. Capell ◽  
P. Vaz-Pires ◽  
P. Gibbs ◽  
...  

A cocktail made up of six bacteria isolated from fish was subjected to various heats (30 °C and 60 °C, 20 s) and chemical preservative stresses, alone and in combination. The chemical preservatives tested were potassium sorbate (PS, 1%), trisodium phosphate (TSP, 10%) and tetrasodium pyrophosphate (TSPP, 3%). Heating the bacteria at 60 °C for 20 s resulted in an average reduction of 2.0 log cfu/mL in the total number. The results showed amarked synergistic effect of heat treatment prior to chemical additives, especially for TSPP and sorbate; no significant effect was evident when chemicals were applied alone. The effect was increased by the application of chemical preservatives, before heating, in the decreasing order TSP, TSPP and sorbate; reductions obtained were more than 5.0, 4.0 and 3.0 log cfu/mL, respectively. The synergistic effect observed was dependent on the type of chemical additives used and also on the order of the treatments. Bacteria identified as Shewanella putrefaciens and Pseudomonas putida were more resistant to heat treatment and Brochothrix thermosphacta and Lactobacillus plantarum were more resistant to TSP treatment. These bacteria in the cocktail showed high sensitivity to combined TSP/heat treatment; bacterial cells identified as Enterobacter spp. were the most resistant to this treatment.


Author(s):  
Diana Carolina Saavedra Plaza ◽  
Carlos Ricardo Soccol ◽  
Miguel Daniel Noseda ◽  
Valcineide Oliveira de Andrade Tanobe ◽  
Oranys Marin ◽  
...  

This study aimed at evaluating effective methods for breaking the hard and insoluble spores of Ganoderma lucidum to recover functional biomolecules. Rupture techniques were evaluated such as manual maceration (RM), maceration with spheres of various materials (BR), and microwave exposure plus maceration with steel/chrome spheres (MBR1). Spore rupture was evaluated using UV-Vis spectroscopy, which showed vibrations of 2955, 1642, 1240, 1080 and 1746 cm-1 corresponding to changes in spore walls. The MBR1 extract contained the largest amounts of carbohydrates (19.80 mg.g-1 spores) and polyphenols (2.21 mg.g-1 spores), whereas the BR extract had higher antioxidant activity (57.22%Inb DPPH). The MBR1 and BR extracts contained 62.2 and 73.5% glucose, respectively. Both methods also involved significant extraction of carbohydrates and proteins. The best way to extract biomolecules from spore walls is to perform a microwave heat treatment and break the walls with steel/chrome spheres; this produces large quantities of carbohydrates with antioxidant properties.


Author(s):  
Putu Hendra Widyadharma ◽  
Muhammad Alief Irham ◽  
Tirta R. Mayangsari ◽  
Octia Floweri ◽  
Akfiny Hasdi Aimon ◽  
...  

2020 ◽  
Vol 22 (3) ◽  
pp. 826-835
Author(s):  
Myung Hwan Hong ◽  
So Young Joo ◽  
Seyul Kim ◽  
Chan Gi Lee ◽  
Dong Wook Kim ◽  
...  

CytoJournal ◽  
2012 ◽  
Vol 9 ◽  
pp. 8 ◽  
Author(s):  
Elsa Beraki ◽  
Thale Kristin Olsen ◽  
Torill Sauer

Background:Protocols for immunocytochemical staining (ICC) andin situhybridization (ISH) of air-dried Diff-Quick or May-Grünwald Giemsa (MGG)-stained smears have been difficult to establish. An increasing need to be able to use prestained slides for ICC and ISH in specific cases led to this study, aiming at finding a robust protocol for both methods.Materials and Methods:The material consisted of MGG- and Diff-Quick-stained smears. After diagnosis, one to two diagnostic smears were stored in the department. Any additional smear(s) containing diagnostic material were used for this study. The majority were fine needle aspirates (FNAC) from the breast, comprising materials from fibroadenomas, fibrocystic disease, and carcinomas. A few were metastatic lesions (carcinomas and malignant melanomas). There were 64 prestained smears. Ten smears were Diff-Quick stained, and 54 were MGG stained. The antibodies used for testing ICC were Ki-67, ER, and PgR, CK MNF116 (pancytokeratin) and E-cadherin. HER-2 Dual SISH was used to test ISH. Citrate, TRS, and TE buffers at pH6 and pH9 were tested, as well as, different heating times, microwave powers and antibody concentrations. The ICC was done on the Dako Autostainer (Dako®, Glostrup, Denmark), and HER-2 Dual SISH was done on the Ventana XT-machine (Ventana / Roche®, Strasbourg, France).Results:Optimal results were obtained with the TE buffer at pH 9, for both ICC and ISH. Antibody concentrations generally had to be higher than in the immunohistochemistry (IHC). The optimal microwave heat treatment included an initial high power boiling followed by low power boiling. No post fixation was necessary for ICC, whereas, 20 minutes post fixation in formalin (4%) was necessary for ISH.Conclusions:Microwave heat treatment, with initial boiling at high power followed by boiling at low power and TE buffer at pH 9 were the key steps in the procedure. Antibody concentrations has to be adapted for each ICC marker. Post fixation in formalin is necessary for ISH.


2013 ◽  
Vol 756-759 ◽  
pp. 4515-4519
Author(s):  
Qi Rong Lu ◽  
Qi Lin Tang ◽  
Lei Wang ◽  
Jie Zhu ◽  
Jian Hui Luo

The heat treatment technology is a weak link in engineering technology in china. For the traditional microwave heat treatment system, the microwave electric field inside the oven can not be well-distributed. In case of the traditional oven heat treatment system, however, the materials dried process is very long. In this paper, we introduce a microwave-oven hybrid heat treatment system related to industrial production line, the proposed with both advantages of high efficiency of microwave and good thermal distribution of the oven, which can be applied for the determination of degree in heat treatment process according to the concentration of the produced gases. Based on the computer and LabVIEW, the control system can control the whole process of heat treatment directly and accurately in real-time.


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