scholarly journals A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores

2020 ◽  
Vol 49 (1) ◽  
Author(s):  
Diana Carolina Saavedra Plaza ◽  
Carlos Ricardo Soccol ◽  
Miguel Daniel Noseda ◽  
Valcineide Oliveira de Andrade Tanobe ◽  
Oranys Marin ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activity ◽  
Comparative Study ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Extraction Techniques ◽  
Microwave Exposure ◽  
Vis Spectroscopy ◽  
Spore Walls ◽  
Microwave Heat

This study aimed at evaluating effective methods for breaking the hard and insoluble spores of Ganoderma lucidum to recover functional biomolecules. Rupture techniques were evaluated such as manual maceration (RM), maceration with spheres of various materials (BR), and microwave exposure plus maceration with steel/chrome spheres (MBR1). Spore rupture was evaluated using UV-Vis spectroscopy, which showed vibrations of 2955, 1642, 1240, 1080 and 1746 cm-1 corresponding to changes in spore walls. The MBR1 extract contained the largest amounts of carbohydrates (19.80 mg.g-1 spores) and polyphenols (2.21 mg.g-1 spores), whereas the BR extract had higher antioxidant activity (57.22%Inb DPPH). The MBR1 and BR extracts contained 62.2 and 73.5% glucose, respectively. Both methods also involved significant extraction of carbohydrates and proteins. The best way to extract biomolecules from spore walls is to perform a microwave heat treatment and break the walls with steel/chrome spheres; this produces large quantities of carbohydrates with antioxidant properties.

scholarly journals Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

2011 ◽  
pp. 279-288 ◽  
Author(s):  
Anita Klaus ◽  
Maja Kozarski ◽  
Miomir Niksic
Keyword(s):  
Antioxidant Activity ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Medicinal Mushrooms ◽  
Water Extracts ◽  
Hot Water Extract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

scholarly journals Antioxidant properties of Philippine Bignay (Antidesma bunius (Linn.) Spreng cv. ‘Common’) flesh and seeds as affected by fruit maturity and heat treatment

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1980-1987
Author(s):  
K.A.T. Castillo-Israel ◽  
K.J.D. Sartagoda ◽  
M.C.R. Illano ◽  
L.E.L. Flandez ◽  
M.C.M. Compendio ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Content ◽  
Common Variety ◽  
Ripe Stage

The effects of maturity (green-unripe, red-ripe, and purple-fully ripe) and heat treatment (blanching at 90±5°C for 2 mins and steaming at 105±5°C for 5 mins) on the antioxidant content and activity of Philippine bignay (Antidesma bunius (Linn.) Spreng cv. ‘common’) flesh and seeds were investigated. The total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC) were determined using the Folin-Ciocalteau assay, aluminum chloride assay, and pH differential technique, respectively, and the antioxidant activity was determined using DPPH, ABTS, and FRAP assays. Results showed that the antioxidant content and activity of bignay increased with maturity. Fully ripe flesh and seeds were found to have the highest antioxidant content and activity regardless of the heat treatment applied. The TPC (8.06±0.15 mg gallic acid equivalents (GAE)/g), TFC (8.10±0.06 mg quercetin equivalents (QE)/g), TAC (4.07±0.03 mg cyanidin-3-glucoside equivalents (CGE)/L extract), and antioxidant activity (ABTS 10.37±0.04 , DPPH 9.60±0.10, FRAP 24.37±0.53 Trolox equivalents (TE)/g) of fully ripe seeds were superior when blanched. On the other hand, the TPC (40.73±0.04 mg GAE/g), TFC (31.54±0.15 mg QE/g), TAC (20.93±0.12 mg CGE/L extract), of fully ripe flesh were superior when not heat-treated while its antioxidant activity (ABTS 34.19±0.19, DPPH 14.01±0.02, FRAP 87.78±1.22 (TE)/g) was highest when blanched but did not vary significantly compared to unheated samples. This study suggested that bignay ‘common’ variety was a good source of antioxidants particularly the flesh at fully ripe stage. Blanching had shown to enhance its antioxidant activities.

Influence of thermal modification and extraction techniques on yield, antioxidant capacity and phytochemical profile of chestnut (Castanea sativa Mill.) wood

Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Immacolata Faraone ◽  
Daniela Russo ◽  
Maurizio D’Auria ◽  
Maria Roberta Bruno ◽  
Paola Cetera ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Castanea Sativa ◽  
Total Content ◽  
Thermal Modification ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Compounds ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction

AbstractNumerous investigations on the antioxidant properties of different plant tissue extracts are available in literature, but few studies on the effect of thermally modified wood on secondary metabolites were carried out up to now. This study highlights the influence of the thermal modification of Castanea sativa Mill. wood on total content of antioxidant compounds and consequently on its antioxidant activity. In addition, a chemical profile by Gas Chromatography–Mass Spectrometry (GC–MS) of the extractives was carried out. Wood of chestnut, thermally modified at 180 °C for 3 h, was used to obtain wood meal which was subjected to different extraction techniques, as maceration extraction (ME), ultrasound assisted extraction (UAE) and accelerated solvent extraction (ASE). The total content of principal antioxidant compounds, such as polyphenols, flavonoids and tannins, as well as the evaluation of antioxidant capacity by using different in vitro assays were determined. Relative Antioxidant Capacity Index (RACI), which is used to compare all antioxidant parameters, has also been applied. The study demonstrated a positive influence on chemical compounds present in C. sativa Mill wood originating from the thermal modification process. Thus, is possible to consider thermal modification as a promising strategy to improve the antioxidant activity of chestnut wood extractives.

Natural Compounds with Antioxidant Properties

2009 ◽  
Vol 415 ◽  
pp. 25-28 ◽  
Author(s):  
Tudor Lupascu ◽  
Gheorghe Duca ◽  
Maria Giurginca ◽  
Pavel Vlad ◽  
Lucian Lupascu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Molecular Mass ◽  
Antioxidant Properties ◽  
Natural Compounds ◽  
High Molecular Mass ◽  
Minimum Concentration ◽  
Natural Substances ◽  
Vis Spectroscopy

Natural substances with polyphenolic structure exhibit remarkable antioxidant properties, being widely used in preventing various diseases. Among these, the oenotanins obtained from grapes seeds are proantocianidinic compounds with high molecular mass, water insoluble and as a result, rather difficult to be utilized. The aim of this paper was to produce their hydrosolubilization and the structure of the product resulted (enoxyl) has been investigated by MS, IR, UV-Vis spectroscopy. The antioxidant activity of oenotanins and enoxyl has been quantified by chemiluminescence technique, and the minimum concentration able to capture completely the free radicals has been also established.

scholarly journals Comparative Study of Antioxidant Property of Coffee and Its Additives

2021 ◽  
pp. 89-96
Author(s):  
Deepti Bajaj ◽  
Suhas Ballal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Comparative Study ◽  
Antioxidant Properties ◽  
Group Analysis ◽  
Antioxidant Property ◽  
Roasted Coffee ◽  
Instant Coffee ◽  
Coffee Beans ◽  
Roasted Coffee Beans

Coffee, one of the most commonly consumed beverages is a very rich source of antioxidants alongside various other health benefits. The roasted beans of coffee are the seed of berry obtained from coffea species. These roasted coffee beans are utilized to prepare coffee. India is today producer of 16 unique varieties of coffee most of which originate from southern India. The goodness of coffee and Ganoderma can do wonders to human health. This study is aimed to have an analysis of antioxidant properties of instant coffee, filter coffee, coffee with Ganoderma extracts and ginger coffee by estimating the Ascorbic Acid equivalents (AAE). The essence of the study is the presence of additions and the antioxidant activity of the coffee samples in their presence. The two infusions used in the study are extracts of Ganoderma and ginger. The study also aims to have a inter group analysis of antioxidant properties of all the samples. The study was conducted using basic colorimetric techniques.

scholarly journals COMPARATIVE STUDY OF POLYPHENOL, FLAVONOID, AND ANTIOXIDANT ACTIVITY OF VARIOUS MEDICINAL PLANTS COLLECTED FROM DIFFERENT ALTITUDES

2021 ◽  
pp. 87-93
Author(s):  
BIGYAN JOSHI ◽  
NETRA LAL BHANDARI ◽  
SUNITA SHRESTHA ◽  
RAJENDRA GYAWALI ◽  
PANNA THAPA
Keyword(s):  
Antioxidant Activity ◽  
Comparative Study ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Mentha Piperita ◽  
Flavonoid Content ◽  
Eupatorium Adenophorum ◽  
Almost All ◽  
Medicinal Flora

Objectives: The main aim of this investigation is to explore the folklore medicinal flora located at Terai and the lesser Himalayan region of Nepal and has a comparative study on phenol, flavonoid, and antioxidant activity that would bring forth a factual basis for its use in the traditional therapy of various health ailments. Methods: Folin-Ciocalteu and Colorimetric aluminum chloride methods were used for the estimation of total polyphenolic and flavonoid content, respectively. Moreover, antioxidant activity was determined by DPPH radical scavenging activity. Results: Among 21 plants collected, Artemesia vulgaris depicted higher (53±0.03 mg GAE/g) and Mimosa pudica (3.7±0.04 mg GAE/g) depicted lower phenolic content whereas the highest flavonoid content is observed in Syzygium cumini and the lowest value in Mentha piperita. Almost all the collected specimen demonstrated antioxidant activity, among which Eupatorium adenophorum and Rhododendron anthopogon demonstrated lower and higher antioxidant activity, respectively. Moreover, phenol and flavonoid content showed a weak correlation with the antioxidant activity indicating the major antioxidant to be different compounds other than phenols or flavonoids. Conclusion: The present study confirms the antioxidant activity of the collected plant specimen and defends its ethnobiological use as a possible natural antioxidant. Furthermore, the result of antioxidant properties encourages their application in medicinal health, functional food, and biopharmaceutics.

scholarly journals Electrochemical Methods for Evaluation of Antioxidant Properties of Propolis Extract Incorporated in Chitosan Nanoparticles

2021 ◽  
Vol 57 (4) ◽  
pp. 96-108
Author(s):  
Luminita Fritea ◽  
Paula Melania Pasca ◽  
Laurian Vlase ◽  
Ana-Maria Gheldiu ◽  
Liviu Moldovan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Properties ◽  
Chitosan Nanoparticles ◽  
Differential Pulse ◽  
Electrochemical Methods ◽  
Propolis Extract ◽  
Vis Spectroscopy ◽  
Pulse Voltammetry ◽  
Nano Formulation

The main purpose of our study was to demonstrate the antioxidant properties of novel propolis nano-formulation, incorporated in spherical chitosan nanoparticles. The electrochemical methods (cyclic voltammetry and differential pulse voltammetry) were successfully applied to propolis extract and nanopropolis formulation, confirming that the content in phenolic acids and flavonoids is responsible for the antioxidant activity of propolis. The quantitative and qualitative results are also supported by high performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectroscopy.

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