scholarly journals Establishing a protocol for immunocytochemical staining and chromogenicin situhybridization of Giemsa and Diff-Quick prestained cytological smears

CytoJournal ◽  
2012 ◽  
Vol 9 ◽  
pp. 8 ◽  
Author(s):  
Elsa Beraki ◽  
Thale Kristin Olsen ◽  
Torill Sauer
Keyword(s):  
Heat Treatment ◽  
Low Power ◽  
High Power ◽  
Immunocytochemical Staining ◽  
Ki 67 ◽  
Fine Needle Aspirates ◽  
Metastatic Lesions ◽  
Her 2 ◽  
Key Steps ◽  
Microwave Heat

Background:Protocols for immunocytochemical staining (ICC) andin situhybridization (ISH) of air-dried Diff-Quick or May-Grünwald Giemsa (MGG)-stained smears have been difficult to establish. An increasing need to be able to use prestained slides for ICC and ISH in specific cases led to this study, aiming at finding a robust protocol for both methods.Materials and Methods:The material consisted of MGG- and Diff-Quick-stained smears. After diagnosis, one to two diagnostic smears were stored in the department. Any additional smear(s) containing diagnostic material were used for this study. The majority were fine needle aspirates (FNAC) from the breast, comprising materials from fibroadenomas, fibrocystic disease, and carcinomas. A few were metastatic lesions (carcinomas and malignant melanomas). There were 64 prestained smears. Ten smears were Diff-Quick stained, and 54 were MGG stained. The antibodies used for testing ICC were Ki-67, ER, and PgR, CK MNF116 (pancytokeratin) and E-cadherin. HER-2 Dual SISH was used to test ISH. Citrate, TRS, and TE buffers at pH6 and pH9 were tested, as well as, different heating times, microwave powers and antibody concentrations. The ICC was done on the Dako Autostainer (Dako®, Glostrup, Denmark), and HER-2 Dual SISH was done on the Ventana XT-machine (Ventana / Roche®, Strasbourg, France).Results:Optimal results were obtained with the TE buffer at pH 9, for both ICC and ISH. Antibody concentrations generally had to be higher than in the immunohistochemistry (IHC). The optimal microwave heat treatment included an initial high power boiling followed by low power boiling. No post fixation was necessary for ICC, whereas, 20 minutes post fixation in formalin (4%) was necessary for ISH.Conclusions:Microwave heat treatment, with initial boiling at high power followed by boiling at low power and TE buffer at pH 9 were the key steps in the procedure. Antibody concentrations has to be adapted for each ICC marker. Post fixation in formalin is necessary for ISH.

Less Power, Greater Conflict

2018 ◽  
Vol 49 (1) ◽  
pp. 47-62 ◽  
Author(s):  
Petra C. Schmid
Keyword(s):  
Low Power ◽  
High Power ◽  
Goal Pursuit ◽  
Goal Conflict ◽  
Personal Goals ◽  
Multiple Goals ◽  
Objective Performance ◽  
The Future ◽  
The Way

Abstract. Power facilitates goal pursuit, but how does power affect the way people respond to conflict between their multiple goals? Our results showed that higher trait power was associated with reduced experience of conflict in scenarios describing multiple goals (Study 1) and between personal goals (Study 2). Moreover, manipulated low power increased individuals’ experience of goal conflict relative to high power and a control condition (Studies 3 and 4), with the consequence that they planned to invest less into the pursuit of their goals in the future. With its focus on multiple goals and individuals’ experiences during goal pursuit rather than objective performance, the present research uses new angles to examine power effects on goal pursuit.

Solution heat treatment with high-power YAG lasers

1993 ◽  
Author(s):  
S. Kimura ◽  
M. Sprenger ◽  
W. Kono ◽  
H. Takahashi ◽  
Y. Tongu
Keyword(s):  
Heat Treatment ◽  
High Power ◽  
Solution Heat Treatment ◽  
Yag Lasers

AlInAs/GaInAs on InP HEMTs for low power supply voltage operation of high power-added efficiency microwave amplifiers

1993 ◽  
Vol 29 (15) ◽  
pp. 1324 ◽  
Author(s):  
L.E. Larson ◽  
M.M. Matloubian ◽  
J.J. Brown ◽  
A.S. Brown ◽  
M. Thompson ◽  
...  
Keyword(s):  
Low Power ◽  
High Power ◽  
Power Supply ◽  
Supply Voltage ◽  
Power Supply Voltage ◽  
Power Added Efficiency ◽  
Microwave Amplifiers

Effect of Power on Moral Judgment

1978 ◽  
Vol 42 (2) ◽  
pp. 387-394
Author(s):  
Russell Hamby
Keyword(s):  
Social Psychology ◽  
Moral Responsibility ◽  
Low Power ◽  
Moral Judgment ◽  
Factorial Design ◽  
High Power ◽  
Moral Judgments ◽  
Need For Power ◽  
Experimenter Effects

Ambiguous effects of power on attributions of moral responsibility for an accident are interpreted to result from the intervening effects of need for power, which is aroused by the anticipation of exercising power over another. 160 subjects from introductory social psychology classes participated in a questionnaire-type experiment comparing effects of high/low carelessness, severe/minor consequences, and high/low power of the attributor in a 2 × 2 × 2 factorial design. In a follow-up experiment 30 subjects were assigned to conditions of high or low power, and their needs for power and moral attributions were measured. High power seemed to arouse need for power, which was curvilinearly related to moral judgments. Those high and low in need for power attributed more moral responsibility to the perpetrator of an accident than those with moderate levels of need for power. The results suggest complicated models of both moral judgments and experimenter effects related to the level or arousal of motivations.

Feeling Guilty and Flattering God: The Mediating Role of Prayer

2021 ◽  
pp. 009164712199242
Author(s):  
Beata Zarzycka ◽  
Kamil Tomaka ◽  
Katarzyna Zając ◽  
Klaudia Marek
Keyword(s):  
Low Power ◽  
High Power ◽  
Mediating Role ◽  
Shame Proneness ◽  
The Relationship ◽  
Significant Mediator

Ingratiation refers to acts of flattery, typically given by a low-power person to a high-power one, performed to gain acceptance and approval. This study investigates ingratiation in the religious setting, asking whether people feeling high levels of guilt or shame tend to manifest such ingratiating behavior toward God. The study aimed to examine the mediating role of prayer in the relationship between guilt and shame and ingratiation toward God. A total of 148 respondents (80 women and 68 men) participated in the study. The Religious Ingratiation Scale, the Content of Prayer Scale, and the Guilt and Shame Proneness Scale were applied to the research. The results showed that feeling guilty increased the tendency to ingratiation toward God. Prayer was the significant mediator in this relationship. People high in guilt tend to flatter God by offering more adoration and fewer repine prayers.

The Pattern of Proline, Glutamic Acid, and Leucine-Rich Protein 1 Expression in Chinese Women with Primary Breast Cancer

2014 ◽  
Vol 29 (1) ◽  
pp. e1-e7 ◽  
Author(s):  
Yanzhi Zhang ◽  
Peng Wang ◽  
Mumu Shi ◽  
Hironobu Sasano ◽  
Monica S.M. Chan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Glutamic Acid ◽  
Primary Breast Cancer ◽  
Chinese Women ◽  
Cancer Prognosis ◽  
Clinicopathological Parameters ◽  
Breast Cancers ◽  
Breast Cancer Patients ◽  
Ki 67 ◽  
Her 2

Background Disparities of biomarkers’ expression in breast cancer across different races and ethnicities have been well documented. Proline, glutamic acid, and leucine-rich protein 1 (PELP1), a novel ER coregulator, has been considered as a promising biomarker of breast cancer prognosis; however, the pattern of PELP1 expression in Chinese women with breast cancer has never been investigated. This study aims to provide useful reference on possible racial or ethnic differences of PELP1 expression in breast cancer by exploring the pattern of PELP1 expression in Chinese women with primary breast cancer. Methods The expression of PELP1 in primary breast cancer samples from 130 Chinese female patients was detected by immunohistochemistry and correlated to other clinicopathological parameters; for comparison, the expression of PELP1 in 26 benign breast fibroadenomas was also examined. Results The overall value of the PELP1 H-score in breast cancer was significantly higher than that in breast fibroadenoma (p<0.001). In our breast cancer patients, the ER/HER-2-positive group had significantly higher PELP1 H-scores than their negative counterparts (p=0.003 for ER and p=0.022 for HER-2); the Ki-67-high group also showed significantly higher PELP1 H-scores than the Ki-67-low group (p=0.008). No significant association between PELP1 H-scores and other clinicopathological parameters was found. Finally, the PELP1 H-score in breast cancers of the luminal B subtype was significantly higher than that in the triple negative subtype (p=0.002). Conclusion Overexpression of PELP1 in Chinese women with primary breast cancer appears to be associated with biomarkers of poor outcome; these results are similar to other reports based on Western populations.

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