Effect of supplementation with tree foliage on in vitro digestibility and fermentation, synthesis of microbial biomass and methane production of cattle diets

Bélanger, G., Savoie, P., Parent, G., Claessens, A., Bertrand, A., Tremblay, G. F., Massé, D., Gilbert, Y. and Babineau, D. 2012. Switchgrass silage for methane production as affected by date of harvest. Can. J. Plant Sci. 92: 1187–1197. Switchgrass (Panicum virgatum L.) is a warm-season grass recognized as a potential biomass crop for energy production in North America, but little information exists on the effect of harvest date on forage and silage characteristics of switchgrass grown in eastern Canada. Our objectives were to determine how harvest date affects several forage and silage characteristics of switchgrass and to relate these to specific methane yield from anaerobically digested switchgrass silage. Switchgrass, seeded in 2002 and 2006, was harvested and ensiled as a one-cut system on three dates in 2007: late July, early September, and early October. The regrowth from the late July harvest was also harvested in early October as a two-cut system. Silage quality parameters [pH, and concentrations of N, N-NH3, total amino acids (TAA), and volatile fatty acids (VFA)] indicated adequate fermentation of all silage samples. In a one-cut system, delaying harvest from late July to early September increased forage dry matter (DM) yield from 9.0 to 11.5 Mg ha−1, forage soluble carbohydrate (SC) concentration from 51 to 85 g kg−1 DM, and silage SC concentration from 13 to 25 g kg−1 DM; delaying harvest from late July to early October decreased forage in vitro true digestibility (IVTD) from 720 to 582 g kg−1 DM, forage in vitro digestibility of the neutral detergent fibre (dNDF) from 590 to 409 g kg−1 DM, and silage acetate concentration from 7.7 to 2.6 g kg−1 DM. The regrowth had higher IVTD and dNDF, lower acid detergent fibre concentration, and higher silage lactate and acetate concentrations than a single harvest taken in early September or early October. The two-cut system and the single harvest in early September produced the highest seasonal forage DM yields (11.5 and 11.9 Mg ha−1). High specific methane yield was (i) correlated with low forage fibre concentration and high DM digestibility and (ii) more correlated to silage concentrations of lactate and acetate than to silage SC concentration.

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Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

Animal Production Science ◽

10.1071/an14988 ◽

2016 ◽

Vol 56 (10) ◽

pp. 1700

Author(s):

J. M. Cantet ◽

D. Colombatto ◽

G. Jaurena

Keyword(s):

Methane Production ◽

Dry Matter ◽

Gas Production ◽

In Vitro Digestibility ◽

Ruminal Fermentation ◽

Neutral Detergent Fibre ◽

Dry Matter Digestibility ◽

Application Rates ◽

The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

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Author Correction: Improving ensiling characteristics by adding lactic acid bacteria modifies in vitro digestibility and methane production of forage-sorghum mixture silage

Scientific Reports ◽

10.1038/s41598-021-87311-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chatchai Kaewpila ◽

Pongsatorn Gunun ◽

Piyawit Kesorn ◽

Sayan Subepang ◽

Suwit Thip‑uten ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Methane Production ◽

In Vitro Digestibility ◽

Forage Sorghum

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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Effect of addition cattle feed supplement on in vitro fermentation, synthesis of microbial biomass, and methane production of rice straw fermentation basal diets

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/888/1/012070 ◽

2021 ◽

Vol 888 (1) ◽

pp. 012070

Author(s):

Ramaiyulis ◽

E Yulia ◽

D K Sari ◽

Nilawati

Keyword(s):

Microbial Biomass ◽

Rice Straw ◽

Methane Production ◽

Gas Production ◽

Feed Supplement ◽

In Vitro Fermentation ◽

Cattle Feed ◽

Concentrate Supplementation ◽

Ruminant Diets

Abstract The objective of this study was to evaluate the influence of supplementation of cattle feed supplement (CFS) and concentrate in ruminant diets based on rice straw fermented (R) on in vitro rumen fermentation, microbial biomass synthesis, and enteric methane production. Five experimental diets were evaluated, consist of R = rice straw fermented 100%, RS = R + CFS 10%, RSC1, 2 and 3 = RS + Concentrate levels 10, 20 and 30 (%DM). Supplementation of CFS increased the gas production (P < 0.05) and highest in treatments RSC1 and 2 (44.09 and 44.87 ml/g substrate, respectively) and was decreased proportions of methane by inhibition rate until 49.80%. Ruminal protozoa population increased by CFS dan concentrate supplementation (P<0,05) and was dominated (>80%) of Entodinium genus. The treatments RS dan RSC1 promoted greater (P < 0.01) microbial biomass synthesis (386.32 and 312.39 mg/g substrate, respectively). In conclusion, the supplementation of CFS and concentrate in ruminant diets based on rice straw fermented can promote a greater synthesis of microbial biomass and mitigation of methane production.

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The Effect of Total or Partial Protected Vegetable Oil Supplementation on In Vitro Digestibility, Feed Fermentability and Energy Efficiency

Buletin Peternakan ◽

10.21059/buletinpeternak.v43i3.43231 ◽

2019 ◽

Vol 43 (3) ◽

Author(s):

Laily Ismatul Faizah ◽

Widiyanto Widiyanto ◽

Anis Muktiani

Keyword(s):

Energy Efficiency ◽

Palm Oil ◽

Methane Production ◽

Corn Oil ◽

Block Design ◽

In Vitro Digestibility ◽

In Vitro Techniques ◽

Dry Matter Digestibility ◽

Ch4 Production

This study was examines the effect of oil (oil palm and corn) combination with the level of protection (total or partial) on feed fermentability, methane production and energy efficiency with in vitro techniques. The experiment was designed using a factorial pattern 2x2, factor A = type of oil (corn and palm) and factor B = oil protection level (total and partial), each treatment combination was repeated 4 times. Data was processed by analyzing various factorial patterns in a randomized block design and if there was an influence between treatments performed by the Dunca test. The results showed that there was no interaction effect (P>0.05) between the types of oil with the level of protection in all parameters, except NH3. Supplementation of palm oil produces total volatyle fatty acids (VFA), acetate and methane (CH4) production higher than corn oil (P<0.05), but the efficiency of converting hexose energy to VFA (ECH) was lower (P<0.05) (76.09 vs 77.80%). Supplementation of total protected oil decreased in the protozoa population, resulting in higher dry matter digestibility (DMD) and organic matter digestibility (OMD), but lower ECH yield compared to partial protected oil supplementation (P<0.05), ie 76.68 vs 77.22%. The conclusions of the study are corn oil produce of ECH higher than palm oil. Partial protection produce better feed fermentability and increasing energy efficiency in the form of decreasing A/P ratio and methane production.

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Effects of source and level of dietary energy supplementation on in vitro digestibility and methane production from tall fescue-based diets

Animal Feed Science and Technology ◽

10.1016/j.anifeedsci.2018.05.010 ◽

2018 ◽

Vol 242 ◽

pp. 41-47 ◽

Cited By ~ 5

Author(s):

R.J. Trotta ◽

J.L. Klotz ◽

D.L. Harmon

Keyword(s):

Tall Fescue ◽

Methane Production ◽

In Vitro Digestibility ◽

Dietary Energy ◽

Energy Supplementation

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Use of essential oils in combination with fibrolytic enzymes to decrease in vitro ruminal methane production

South African Journal of Animal Science ◽

10.4314/sajas.v50i5.5 ◽

2021 ◽

Vol 50 (5) ◽

Author(s):

T.C. Nel ◽

A. Hassen ◽

A.M. Akanmu ◽

F.A. Adejoro

Keyword(s):

Organic Matter ◽

Essential Oils ◽

Methane Production ◽

Gas Production ◽

In Vitro Digestibility ◽

Diallyl Disulfide ◽

Cinnamon Oil ◽

Garlic Oil ◽

Organic Matter Digestibility

Exogenous enzymes and essential oils (EOs) have both improved fibre digestion in ruminants, but the effect on ruminal fermentation and methane emission of combining these additives requires further evaluation. Various EOs were used in combination with an enzyme in an in vitro gas production system to measure organic matter digestibility and methane after 48 hours of incubation of Eragrostis curvula. The treatments consisted of T1) unaugmented control; T2) enzyme (cellulose-xylanase mixture, 1:1 w/w, 1.5 mL/g DM); T3) enzyme combined with Next Enhance® (cinnamaldehyde with diallyl disulfide and diallyl trisulfide, 43:3.85 w/wt); T4) enzyme and cinnamon oil; T5) enzyme and garlic oil; and T6) enzyme with cinnamon and garlic oil, 1:1 w/w). At 2, 4, 8, 12, 24, 36, and 48 hours of incubation, gas production was measured, and gas samples were analysed for methane concentration. The inclusion of additives affected cumulative gas production (P <0.05), but only slightly affected methane production (P =0.05) after 48 hours of incubation. The addition of enzymes increased both gas and methane production. T5 and T6 both reduced gas production compared with T2. Combining EOs with an enzyme reduced methane production compared with T2 after 48 hours of incubation. The additives tended to increase in vitro organic matter digestibility (P =0.09), although they showed a tendency towards reduced methane production per unit of organic matter digested (P =0.07). Further studies on these enzyme and EO combinations under different doses and substrates are required to validate their efficacy for ruminant production. Keywords: cinnamon oil, cinnamaldehyde, garlic oil, gas production, glucanase, in vitro digestibility, xylanase

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