Combined use of killer biotype and mtDNA-RFLP patterns in a Patagonian wine Saccharomyces cerevisiae diversity study

2005 ◽  
Vol 89 (1) ◽  
pp. 147-156 ◽  
Author(s):  
Christian A Lopes ◽  
Teresa L Lavalle ◽  
Amparo Querol ◽  
Adriana C Caballero
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Combined Use ◽  
Diversity Study ◽  
Mtdna Rflp

scholarly journals Combined Use of Lachancea thermotolerans and Schizosaccharomyces pombe in Winemaking: A Review

2020 ◽  
Vol 8 (5) ◽  
pp. 655 ◽  
Author(s):  
Santiago Benito
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Schizosaccharomyces Pombe ◽  
Oenococcus Oeni ◽  
Quality Parameters ◽  
Ethyl Carbamate ◽  
Wine Quality ◽  
The Past ◽  
Combined Use ◽  
First Time ◽  
Lachancea Thermotolerans

The combined use of Lachancea thermotolerans and Schizosaccharomyces pombe is a new winemaking biotechnology that aims to solve some modern industrial oenology problems related to warm viticulture regions. These areas are characterized for producing musts with high levels of sugar that can potentially be converted into wines with elevated ethanol contents, which are usually associated with high pH levels. This biotechnology was reported for the first time in 2015, and since then, several scientific articles have been published regarding this topic. These reported scientific studies follow an evolution similar to that performed in the past for Saccharomyces cerevisiae and Oenococcus oeni; they start by reporting results for basic winemaking parameters at the beginning, later continuing with more advanced parameters. This review compares the results of different researchers that have applied this new biotechnology and have studied wine quality parameters such as ethanol, glycerol, malic acid, lactic acid, amino acids, aroma compounds, or anthocyanins. It is shown that the new biotechnology is repeatedly reported to solve specific winemaking problems such as the lack of acidity, biogenic amines, ethyl carbamate, or undesirable color losses. Such results highlight this biotechnology as a promising option for warm viticulture areas.

Combined use of thermo-ultrasound and cinnamon leaf essential oil to inactivate Saccharomyces cerevisiae in natural orange and pomegranate juices

LWT ◽  
2016 ◽  
Vol 73 ◽  
pp. 140-146 ◽  
Author(s):  
Marta Sánchez-Rubio ◽  
Amaury Taboada-Rodríguez ◽  
Rita Cava-Roda ◽  
Antonio López-Gómez ◽  
Fulgencio Marín-Iniesta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Essential Oil ◽  
Combined Use ◽  
Leaf Essential Oil

scholarly journals Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Satoshi Okada ◽  
Goro Doi ◽  
Shitomi Nakagawa ◽  
Emiko Kusumoto ◽  
Takashi Ito
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genome Editing ◽  
Region Of Interest ◽  
Yeast Genome ◽  
Yeast Genetics ◽  
Reading Frame ◽  
Protospacer Adjacent Motif ◽  
Combined Use ◽  
Genome Manipulation ◽  
Complete Deletion

Genome editing using the CRISPR/Cas system has been implemented for various organisms and becomes increasingly popular even in the genetically tractable budding yeast Saccharomyces cerevisiae. Since each CRISPR/Cas system recognizes only the sequences flanked by its unique protospacer adjacent motif (PAM), a certain single system often fails to target a region of interest due to the lack of PAM, thus necessitating the use of another system with a different PAM. Three CRISPR/Cas systems with distinct PAMs, namely SpCas9, SaCas9, and AsCas12a, have been successfully used in yeast genome editing and their combined use should expand the repertoire of editable targets. However, currently available plasmids for these systems were individually developed under different design principles, thus hampering their seamless use in the practice of genome editing. Here we report a series of Golden Gate Assembly-compatible backbone vectors designed under a unified principle to exploit the three CRISPR/Cas systems in yeast genome editing. We also created a software to assist the design of genome-editing plasmids for individual target sequences using the backbone vectors. Genome editing with these plasmids demonstrated practically sufficient efficiency in both insertion of gene fragments to essential genes and complete deletion of an open reading frame. The backbone vectors with the software would thus provide a versatile toolbox to facilitate the seamless use of SpCas9, SaCas9, and AsCas12a in various types of genome manipulation, especially those that are difficult to perform with conventional techniques in yeast genetics.

Synergistic Inactivation Mechanism of Combined Plasma-Activated Water and Mild Heat against Saccharomyces cerevisiae

2020 ◽  
Vol 83 (8) ◽  
pp. 1307-1314 ◽  
Author(s):  
RONG ZHANG ◽  
YUNFANG MA ◽  
DI WU ◽  
LIUMIN FAN ◽  
YANHONG BAI ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Membrane ◽  
Combined Treatment ◽  
Oxygen Species ◽  
Simultaneous Application ◽  
Mild Heat ◽  
Combined Use ◽  
Inactivation Mechanism ◽  
The Individual ◽  
Plasma Activated Water

ABSTRACT This study aimed to elucidate the mechanism of synergistic inactivation of Saccharomyces cerevisiae by the combined use of plasma-activated water (PAW) and mild heat (40 to 50°C). A reduction of 4.40 log CFU/mL in S. cerevisiae was observed after the synergistic combination of PAW and mild heat at 50°C for 6 min, whereas the individual treatments of PAW at 25°C and mild heat at 50°C for 6 min resulted in a reduction of 0.27 and 1.92 log CFU/mL, respectively. The simultaneous application of PAW and mild heat caused significant increases in membrane permeability, resulting in the leakage of intracellular components (such as nucleic acids and proteins) and increased uptake of propidium iodide. The combined treatment of PAW and mild heat also resulted in significant increases in the intracellular levels of reactive oxygen species and disruption of mitochondrial membrane potential in S. cerevisiae cells. In summary, this study illustrates the potential of PAW treatment combined with mild heat to rapidly inactivate microorganisms in food products. HIGHLIGHTS

scholarly journals Human Mitochondrial Pathologies of the Respiratory Chain and ATP Synthase: Contributions from Studies of Saccharomyces cerevisiae

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 304
Author(s):  
Leticia V. R. Franco ◽  
Luca Bremner ◽  
Mario H. Barros
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Respiratory Chain ◽  
Atp Synthase ◽  
Molecular Genetic ◽  
Nuclear Gene ◽  
Mitochondrial Diseases ◽  
Basic Knowledge ◽  
Yeast Saccharomyces Cerevisiae ◽  
Catalytic Subunits ◽  
Combined Use

The ease with which the unicellular yeast Saccharomyces cerevisiae can be manipulated genetically and biochemically has established this organism as a good model for the study of human mitochondrial diseases. The combined use of biochemical and molecular genetic tools has been instrumental in elucidating the functions of numerous yeast nuclear gene products with human homologs that affect a large number of metabolic and biological processes, including those housed in mitochondria. These include structural and catalytic subunits of enzymes and protein factors that impinge on the biogenesis of the respiratory chain. This article will review what is currently known about the genetics and clinical phenotypes of mitochondrial diseases of the respiratory chain and ATP synthase, with special emphasis on the contribution of information gained from pet mutants with mutations in nuclear genes that impair mitochondrial respiration. Our intent is to provide the yeast mitochondrial specialist with basic knowledge of human mitochondrial pathologies and the human specialist with information on how genes that directly and indirectly affect respiration were identified and characterized in yeast.

scholarly journals Effect of the use of Yarrowia lipolytica and Saccharomyces cerevisiae yeast with a probiotic in the diet of turkeys on their gut microbiota and immunity

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 174-182
Author(s):  
A Czech ◽  
I Sembratowicz ◽  
G Zieba
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gut Microbiota ◽  
Beneficial Effect ◽  
Yarrowia Lipolytica ◽  
Control Group ◽  
Yeast Saccharomyces Cerevisiae ◽  
E Coli ◽  
Combined Use ◽  
Number Of Microorganisms ◽  
Fodder Yeast

An experiment was carried out to determine whether the yeast species Yarrowia lipolytica added to compound feeds for turkeys would have a more beneficial effect on their immunity and gut microflora composition than the commonly used species Saccharomyces cerevisiae. An additional aim of the study was to test whether the addition of a probiotic (Bacillus licheniformis and Bacillus subtilis) to the feed containing Yarrowia lipolytica or Saccharomyces cerevisiae yeast would enhance their effect. The experiment was carried out on growing turkey hens aged 7 to 112 days and randomly divided into six groups (each n = 80). The birds in the control group (C) and group P were fed standard feeds, but group P additionally received a probiotic. Groups Y and YP received the feed containing the Yarrowia lipolytica fodder yeast, and the probiotic for the YP group. Similarly, in groups S and SP, the turkeys received the feed with the Saccharomyces cerevisiae fodder yeast, and the probiotic was additionally added to the feed for the SP group. Yarrowia lipolytica yeast can be an alternative to the commonly used yeast Saccharomyces cerevisiae in turkey feeds. Yarrowia lipolytica favourably influenced the intestinal microbiota (reduced the number of microorganisms – P < 0.001, fungi – P < 0.001, and coliforms – P < 0.001, including E. coli), and stimulated erythropoiesis (increased Hb content – P = 0.049 and RBC count – P = 0.027; increased Ht – P < 0.001) and immune mechanisms (increased the %pc – P = 0.021, NBT value – P = 0.013 and lysozyme content – P = 0.013; decreased IgM concentration – P = 0.049). The combined use of a probiotic with yeast, particularly Yarrowia lipolytica, has a more beneficial effect on the gut microbiota than the use of Yarrowia lipolytica alone. The combined use of a probiotic with a yeast, particularly Yarrowia lipolytica, has a more beneficial effect on the gut microbiota than the use of Yarrowia lipolytica alone.

Saccharomyces cerevisiae strains associated with the production of cachaça: identification and characterization by traditional and molecular methods (PCR, PFGE and mtDNA-RFLP)

2008 ◽  
Vol 24 (11) ◽  
pp. 2705-2712 ◽  
Author(s):  
Taís Letícia Bernardi ◽  
Gilberto Vinícius de Melo Pereira ◽  
Patricia Gomes Cardoso ◽  
Eustáquio Souza Dias ◽  
R. F. Schwan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Methods ◽  
Identification And Characterization ◽  
Mtdna Rflp

scholarly journals Effect of the Use of Yarrowia lipolytica or Saccharomyces cerevisiae Yeast with a Probiotic in the Diet of Turkey Hens on Growth Performance and Gut Histology

2020 ◽  
Vol 20 (3) ◽  
pp. 1047-1063 ◽  
Author(s):  
Anna Czech ◽  
Malwina Merska-Kazanowska ◽  
Katarzyna Ognik ◽  
Grzegorz Zięba
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Beneficial Effect ◽  
Growth Performance ◽  
Yarrowia Lipolytica ◽  
Yeast Species ◽  
Control Group ◽  
Intestinal Histology ◽  
Combined Use ◽  
Fodder Yeast ◽  
Effect On Growth

AbstractThe aim of the study was to determine whether the alternative yeast species Yarrowia lipolytica in turkey feed would have a more beneficial effect on growth performance and intestinal histology than the commonly used species Saccharomyces cerevisiae. An additional objective of the study was to test whether the addition of a probiotic to feed containing Yarrowia lipolytica or Saccharomyces cerevisiae yeast would enhance its effect on growth performance and intestinal histology in turkeys. The experiment was carried out on 480 turkey hens randomly divided into six groups. Birds from the control group (C) and group P were fed standard feeds but group P additionally received a probiotic (0.05%). Groups Y and YP received feed containing Yarrowia lipolytica fodder yeast (3%), and the YP group received also the probiotic (0.05%). Similarly, in groups S and SP, the turkeys received feed with Saccharomyces cerevisiae fodder yeast (3%), and for the SP group the probiotic was added to the feed (0.05%). Yarrowia lipolytica yeast added in the amount of 3% to the turkey feed may be an alternative to the commonly used Saccharomyces cerevisiae yeast, because it improved growth performance, and above all, had a more beneficial effect on intestinal histology. The use of Yarrowia lipolytica alone can be beneficial for growth performance, while the combined use of 3% Yarrowia lipolytica in the feed and a 0.05% addition of a probiotic containing Bacillus licheniformis and Bacillus subtilis has a more beneficial effect on gastrointestinal histology.

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