Gelam honey promotes ex vivo corneal fibroblasts wound healing

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

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In Vivo and ex Vivo Approaches to Studying the Biomechanical Properties of Healing Wounds in Rat Skin

Journal of Biomechanical Engineering ◽

10.1115/1.4025109 ◽

2013 ◽

Vol 135 (10) ◽

Cited By ~ 14

Author(s):

Clare Y. L. Chao ◽

Gabriel Y. F. Ng ◽

Kwok-Kuen Cheung ◽

Yong-Ping Zheng ◽

Li-Ke Wang ◽

...

Keyword(s):

Wound Healing ◽

Ex Vivo ◽

Normal Skin ◽

Biomechanical Properties ◽

Skin Wound ◽

Sprague Dawley ◽

Rat Skin ◽

Mechanical Stiffness ◽

Air Jet

An evaluation of wound mechanics is crucial in reflecting the wound healing status. The present study examined the biomechanical properties of healing rat skin wounds in vivo and ex vivo. Thirty male Sprague-Dawley rats, each with a 6 mm full-thickness circular punch biopsied wound at both posterior hind limbs were used. The mechanical stiffness at both the central and margins of the wound was measured repeatedly in five rats over the same wound sites to monitor the longitudinal changes over time of before wounding, and on days 0, 3, 7, 10, 14, and 21 after wounding in vivo by using an optical coherence tomography-based air-jet indentation system. Five rats were euthanized at each time point, and the biomechanical properties of the wound tissues were assessed ex vivo using a tensiometer. At the central wound bed region, the stiffness measured by the air-jet system increased significantly from day 0 (17.2%), peaked at day 7 (208.3%), and then decreased progressively until day 21 (40.2%) as compared with baseline prewounding status. The biomechanical parameters of the skin wound samples measured by the tensiometer showed a marked reduction upon wounding, then increased with time (all p < 0.05). On day 21, the ultimate tensile strength of the skin wound tissue approached 50% of the normal skin; while the stiffness of tissue recovered at a faster rate, reaching 97% of its prewounded state. Our results suggested that it took less time for healing wound tissues to recover their stiffness than their maximal strength in rat skin. The stiffness of wound tissues measured by air-jet could be an indicator for monitoring wound healing and contraction.

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FAK Inhibition Attenuates Corneal Fibroblast Differentiation In Vitro

Biomolecules ◽

10.3390/biom11111682 ◽

2021 ◽

Vol 11 (11) ◽

pp. 1682

Author(s):

Vincent Yeung ◽

Sriniwas Sriram ◽

Jennifer A. Tran ◽

Xiaoqing Guo ◽

Audrey E. K. Hutcheon ◽

...

Keyword(s):

Wound Healing ◽

Transforming Growth Factor ◽

Smooth Muscle Actin ◽

Myofibroblast Differentiation ◽

Corneal Scarring ◽

Corneal Fibroblast ◽

Corneal Fibroblasts ◽

Corneal Wound ◽

Tgf Β1

Corneal fibrosis (or scarring) occurs in response to ocular trauma or infection, and by reducing corneal transparency, it can lead to visual impairment and blindness. Studies highlight important roles for transforming growth factor (TGF)-β1 and -β3 as modulators in corneal wound healing and fibrosis, leading to increased extracellular matrix (ECM) components and expression of α-smooth muscle actin (αSMA), a myofibroblast marker. In this study, human corneal fibroblasts (hCF) were cultured as a monolayer culture (2D) or on poly-transwell membranes to generate corneal stromal constructs (3D) that were treated with TGF-β1, TGF-β3, or TGF-β1 + FAK inhibitor (FAKi). Results show that hCF 3D constructs treated with TGF-β1 or TGF-β3 impart distinct effects on genes involved in wound healing and fibrosis—ITGAV, ITGB1, SRC and ACTA2. Notably, in the 3D construct model, TGF-β1 enhanced αSMA and focal adhesion kinase (FAK) protein expression, whereas TGF-β3 did not. In addition, in both the hCF 2D cell and 3D construct models, we found that TGF-β1 + FAKi attenuated TGF-β1-mediated myofibroblast differentiation, as shown by abrogated αSMA expression. This study concludes that FAK signaling is important for the onset of TGF-β1-mediated myofibroblast differentiation, and FAK inhibition may provide a novel beneficial therapeutic avenue to reduce corneal scarring.

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Development of Curcumin loaded chitosan polymer based nanoemulsion gel: In vitro, ex vivo evaluation and in vivo wound healing studies

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2017.03.066 ◽

2017 ◽

Vol 101 ◽

pp. 569-579 ◽

Cited By ~ 30

Author(s):

Lydia Thomas ◽

Foziyah Zakir ◽

Mohd. Aamir Mirza ◽

Md. Khalid Anwer ◽

Farhan Jalees Ahmad ◽

...

Keyword(s):

Wound Healing ◽

Ex Vivo ◽

Nanoemulsion Gel

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A comparative study of the wound healing properties of Gelam honey and silver sulfadiazine in diabetic rats

2014 IEEE Conference on Biomedical Engineering and Sciences (IECBES) ◽

10.1109/iecbes.2014.7047495 ◽

2014 ◽

Cited By ~ 3

Author(s):

R. M. Zohdi ◽

S. M. Mukhtar ◽

S. Said ◽

N. A. M. Azmi ◽

A. A. Ali

Keyword(s):

Wound Healing ◽

Comparative Study ◽

Diabetic Rats ◽

Silver Sulfadiazine ◽

Gelam Honey

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932 Development of an early wound model in human ex vivo skin explant as a preclinical model of wound healing

Journal of Investigative Dermatology ◽

10.1016/j.jid.2019.03.1008 ◽

2019 ◽

Vol 139 (5) ◽

pp. S161

Author(s):

A.V. Caserta ◽

J.E. Neil ◽

J. Volmer ◽

L. Ring ◽

J. Lenn ◽

...

Keyword(s):

Wound Healing ◽

Ex Vivo ◽

Preclinical Model ◽

Wound Model ◽

Early Wound

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Hyperforin/HP-β-Cyclodextrin Enhances Mechanosensitive Ca2+ Signaling in HaCaT Keratinocytes and in Atopic Skin Ex Vivo Which Accelerates Wound Healing

BioMed Research International ◽

10.1155/2017/8701801 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 11

Author(s):

Hiroya Takada ◽

Jun Yonekawa ◽

Masami Matsumoto ◽

Kishio Furuya ◽

Masahiro Sokabe

Keyword(s):

Wound Healing ◽

Ex Vivo ◽

Atp Release ◽

Aqueous Solubility ◽

P2y Receptors ◽

Hacat Keratinocytes ◽

Wound Edge ◽

Atopic Skin ◽

Intracellular Ca ◽

Mechanical Stretching

Cutaneous wound healing is accelerated by mechanical stretching, and treatment with hyperforin, a major component of a traditional herbal medicine and a known TRPC6 activator, further enhances the acceleration. We recently revealed that this was due to the enhancement of ATP-Ca2+ signaling in keratinocytes by hyperforin treatment. However, the low aqueous solubility and easy photodegradation impede the topical application of hyperforin for therapeutic purposes. We designed a compound hydroxypropyl-β-cyclodextrin- (HP-β-CD-) tetracapped hyperforin, which had increased aqueous solubility and improved photoprotection. We assessed the physiological effects of hyperforin/HP-β-CD on wound healing in HaCaT keratinocytes using live imaging to observe the ATP release and the intracellular Ca2+ increase. In response to stretching (20%), ATP was released only from the foremost cells at the wound edge; it then diffused to the cells behind the wound edge and activated the P2Y receptors, which caused propagating Ca2+ waves via TRPC6. This process might facilitate wound closure, because the Ca2+ response and wound healing were inhibited in parallel by various inhibitors of ATP-Ca2+ signaling. We also applied hyperforin/HP-β-CD on an ex vivo skin model of atopic dermatitis and found that hyperforin/HP-β-CD treatment for 24 h improved the stretch-induced Ca2+ responses and oscillations which failed in atopic skin.

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Active antioxidants in ex-vivo examination of burn wound healing by means of IR and Raman spectroscopies—Preliminary comparative research

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2016.10.046 ◽

2017 ◽

Vol 173 ◽

pp. 924-930 ◽

Cited By ~ 7

Author(s):

Anna Pielesz ◽

Dorota Biniaś ◽

Ewa Sarna ◽

Rafał Bobiński ◽

Marek Kawecki ◽

...

Keyword(s):

Wound Healing ◽

Comparative Research ◽

Ex Vivo ◽

Burn Wound ◽

Burn Wound Healing ◽

Ir And Raman Spectroscopies ◽

Ir And Raman

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Development and Evaluation of Alginate Membranes with Curcumin-Loaded Nanoparticles for Potential Wound-Healing Applications

Pharmaceutics ◽

10.3390/pharmaceutics11080389 ◽

2019 ◽

Vol 11 (8) ◽

pp. 389 ◽

Cited By ~ 10

Author(s):

Mónica C. Guadarrama-Acevedo ◽

Raisa A. Mendoza-Flores ◽

María L. Del Prado-Audelo ◽

Zaida Urbán-Morlán ◽

David M. Giraldo-Gomez ◽

...

Keyword(s):

Wound Healing ◽

Wound Dressing ◽

Clinical Performance ◽

Ex Vivo ◽

High Capacity ◽

Wound Dressings ◽

In Vivo Studies ◽

Potential Use

Non-biodegradable materials with a low swelling capacity and which are opaque and occlusive are the main problems associated with the clinical performance of some commercially available wound dressings. In this work, a novel biodegradable wound dressing was developed by means of alginate membrane and polycaprolactone nanoparticles loaded with curcumin for potential use in wound healing. Curcumin was employed as a model drug due to its important properties in wound healing, including antimicrobial, antifungal, and anti-inflammatory effects. To determine the potential use of wound dressing, in vitro, ex vivo, and in vivo studies were carried out. The novel membrane exhibited the diverse functional characteristics required to perform as a substitute for synthetic skin, such as a high capacity for swelling and adherence to the skin, evidence of pores to regulate the loss of transepidermal water, transparency for monitoring the wound, and drug-controlled release by the incorporation of nanoparticles. The incorporation of the nanocarriers aids the drug in permeating into different skin layers, solving the solubility problems of curcumin. The clinical application of this system would cover extensive areas of mixed first- and second-degree wounds, without the need for removal, thus decreasing the patient’s discomfort and the risk of altering the formation of the new epithelium.

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