Impact of benzimidazole resistance on fitness parameters and fumonisin B1 production in Fusarium verticillioides (Sacc) Niremberg

2021 ◽  
Author(s):  
A. A. Malandrakis ◽  
D. Karamanou ◽  
F. Flouri
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Benzimidazole Resistance ◽  
Fitness Parameters

scholarly journals Fusarium verticillioides FvPex8 is a key component of peroxisomal docking/translocation module that serves important roles in fumonisin biosynthesis but not in virulence

2020 ◽  
Author(s):  
Wenying Yu ◽  
Mei Lin ◽  
Minghui Peng ◽  
Huijuan Yan ◽  
Jie Zhou ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Gene Expression Profiles ◽  
Deletion Mutants ◽  
Altered Expression ◽  
The Core ◽  
Metabolic Functions ◽  
Peroxisome Membrane

AbstractPeroxisomes are ubiquitous organelles in eukaryotic cells that fulfill various important metabolic functions. In this study, we investigated the role of Docking/Translocation Module (DTM) peroxins, mainly FvPex8, FvPex13, FvPex14, and FvPex33, in Fusarium verticillioides virulence and fumonisin B1 (FB1) biosynthesis. Protein interaction experiments suggested that FvPex13 serves as the core subunit of F. verticillioides DTM. When we generated gene deletion mutants (ΔFvpex8, ΔFvpex13, ΔFvpex14, ΔFvpex33, ΔFvpex33/14) and examined whether the expression of other peroxin genes were affected in the DTM mutants, ΔFvpex8 strain showed most drastic changes to PEX gene expression profiles. Deletion mutants exhibited disparity in carbon source utilization and defect in cell wall integrity when stress agents were applied. Under nutrient starvation, mutants also showed higher levels of lipid droplet accumulation. Notably, ΔFvpex8 mutant showed significant FB1 reduction and altered expression of FUM1 and FUM19 genes. However, FvPex13 was primarily responsible for virulence, while ΔFvpex33/14 double mutant also showed virulence defect. In summary, our study suggests that FvPex13 is the core component of DTM, regulating peroxisome membrane biogenesis as well as PTS1- and PTS2-mediated transmembrane cargo transportation. Importantly, we predict FvPex8 as a key component in DTM that affects peroxisome function in FB1 biosynthesis in F. verticillioides.

scholarly journals Effect of Temperature, Water Activity and Carbon Dioxide on Fungal Growth and Mycotoxin Production of Acclimatised Isolates of Fusarium verticillioides and F. graminearum

Toxins ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 478 ◽  
Author(s):  
Ladi Peter Mshelia ◽  
Jinap Selamat ◽  
Nik Iskandar Putra Samsudin ◽  
Mohd Y. Rafii ◽  
Noor-Azira Abdul Mutalib ◽  
...  
Keyword(s):  
Climate Change ◽  
Carbon Dioxide ◽  
Elevated Temperature ◽  
Water Activity ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Fungal Growth ◽  
Effect Of Temperature ◽  
Mycotoxin Production ◽  
Co2 Levels

Climate change is primarily manifested by elevated temperature and carbon dioxide (CO2) levels and is projected to provide suitable cultivation grounds for pests and pathogens in the otherwise unsuitable regions. The impacts of climate change have been predicted in many parts of the world, which could threaten global food safety and food security. The aim of the present work was therefore to examine the interacting effects of water activity (aw) (0.92, 0.95, 0.98 aw), CO2 (400, 800, 1200 ppm) and temperature (30, 35 °C and 30, 33 °C for Fusarium verticillioides and F. graminearum, respectively) on fungal growth and mycotoxin production of acclimatised isolates of F. verticillioides and F. graminearum isolated from maize. To determine fungal growth, the colony diameters were measured on days 1, 3, 5, and 7. The mycotoxins produced were quantified using a quadrupole-time-of-flight mass spectrometer (QTOF-MS) combined with ultra-high-performance liquid chromatography (UHPLC) system. For F. verticillioides, the optimum conditions for growth of fumonisin B1 (FB1), and fumonisin B2 (FB2) were 30 °C + 0.98 aw + 400 ppm CO2. These conditions were also optimum for F. graminearum growth, and zearalenone (ZEA) and deoxynivalenol (DON) production. Since 30 °C and 400 ppm CO2 were the baseline treatments, it was hence concluded that the elevated temperature and CO2 levels tested did not seem to significantly impact fungal growth and mycotoxin production of acclimatised Fusarium isolates. To the best of our knowledge thus far, the present work described for the first time the effects of simulated climate change conditions on fungal growth and mycotoxin production of acclimatised isolates of F. verticillioides and F. graminearum.

Impact of ethnic pre-harvest practices on the occurrence of Fusarium verticillioides and fumonisin B1 in maize fields from Vietnam

Food Control ◽  
2021 ◽  
Vol 120 ◽  
pp. 107567
Author(s):  
Minh Trang Tran ◽  
Maarten Ameye ◽  
Lien Thi-Kim Phan ◽  
Frank Devlieghere ◽  
Sarah De Saeger ◽  
...  
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1

scholarly journals In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

2020 ◽  
Vol 8 (2) ◽  
pp. 241 ◽  
Author(s):  
Giovanni Beccari ◽  
Łukasz Stępień ◽  
Andrea Onofri ◽  
Veronica M. T. Lattanzio ◽  
Biancamaria Ciasca ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Geographical Origin ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Genetic Profile ◽  
Mediterranean Countries ◽  
Intergenic Regions ◽  
Fumonisin B2 ◽  
High Level

Investigating the in vitro fumonisin biosynthesis and the genetic structure of Fusarium verticillioides populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 F. verticillioides strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B1, fumonisin B2 and fumonisin B3 and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03–69.84 μg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent FUM1 gene and of intergenic regions FUM6-FUM7 and FUM7-FUM8 were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains.

scholarly journals Comparison of the Performances of Two Aptamers on a Colorimetric Assay for the Quantification of Fumonisin B1

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 19
Author(s):  
Vicente Antonio Mirón-Mérida ◽  
Yadira González-Espinosa ◽  
Yun Yun Gong ◽  
Yuan Guo ◽  
Francisco M. Goycoolea
Keyword(s):  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Detection Methods ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Subsequent Addition ◽  
Selection Of ◽  
Analytical Detection

Fumonisin B1 (FB1), a mycotoxin commonly produced by Fusarium verticillioides and classified as a group 2B hazard, has been identified in various food products; hence, sensitive and rapid analytical detection methods are needed. Since the first reported aptamer (96 nt ssDNA) for the highly specific molecular recognition of FB1, only 30 aptamer-based biosensors have been published. A critical point, yet commonly overlooked during the design of aptasensors, is the selection of the binding buffer. In this work, a colorimetric assay was designed by incubating a folded aptamer with FB1 and the subsequent addition of gold nanoparticles (AuNPs). The changes in the aggregation profile of AuNPs by a 40 nt aptamer and a 96 nt aptamer were tested after the addition of FB1 under different buffer conditions, where the incubation with Tris-HCl and MgCl2 exhibited the most favorable performances. The assay with the longest aptamer was specific to FB1 and comparable to other aptasensors with a limit of detection (LOD) of 3 ng/mL (A650/520 ratio). Additionally, the application of asymmetric-flow field-flow fractionation (AF4) with multidetection allowed for the analysis of the peak area (λ) and multi-angle light scattering (MALS) with LODs of up to the fg/mL level.

scholarly journals Fumonisin Production by Fusarium verticillioides in Maize Genotypes Cultivated in Different Environments

Toxins ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 215 ◽  
Author(s):  
Oelton Ferreira Rosa Junior ◽  
Mateus Sunti Dalcin ◽  
Vitor L. Nascimento ◽  
Fernando Machado Haesbaert ◽  
Talita Pereira de Souza Ferreira ◽  
...  
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Human Consumption ◽  
Ear Rot ◽  
Maize Hybrids ◽  
High Severity ◽  
Maize Genotypes ◽  
Spray Inoculation ◽  
Corn Products ◽  
High Concentrations

Fumonisins are mycotoxins (MTs) produced mainly by the fungus Fusarium verticillioides, the main pathogens of maize which cause ear rot. The aim of this work was to evaluate some factors that may lead to high fumonisin production by F. verticillioides in maize grains, correlating the pathogen inoculation method with different genotypes grown in four Brazilian states. Experiments were conducted in 2015–2016 in maize crops from experimental maize fields located in four distinct states of Brazil. Results showed that contamination by fumonisin mycotoxins occurred even on symptomatic or asymptomatic grains. In all municipalities, the samples showed levels of fumonisin B1 that were higher than would be tolerable for the human consumption of corn products (the current tolerance limit for fumonisin is 1.5 μg g−1). High severity of grains infected with F. verticillioides does not always show high concentrations of fumonisins. Environments with higher temperatures may influence the production of high concentrations of fumonisin in maize hybrids. Spray inoculation methods and inoculation at the center of spikes did not influence fumonisin concentrations. Results showed that the hybrids P3630H, P32R48 and P3250 presented higher disease severity, as well as higher mycotoxin levels in the studied locations with higher temperatures.

scholarly journals Potential Application of Lactic Acid Bacteria to Reduce Aflatoxin B1 and Fumonisin B1 Occurrence on Corn Kernels and Corn Ears

Toxins ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 21 ◽  
Author(s):  
Tiago de Melo Nazareth ◽  
Carlos Luz ◽  
Raquel Torrijos ◽  
Juan Manuel Quiles ◽  
Fernando Bittencourt Luciano ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Health Concern ◽  
Economic Losses ◽  
Antifungal Effect ◽  
Minimum Fungicidal Concentration ◽  
Sensory Defects ◽  
Corn Kernels

Fungal spoilage is an important issue for the food industry, leading to food sensory defects, food waste, economic losses and public health concern through the production of mycotoxins. Concomitantly, the search for safer natural products has gained importance since consumers began to look for less processed and chemically treated foods. In this context, the aim of this study was to evaluate the antifungal and antimycotoxigenic effect of seven strains of Lactobacillus plantarum. Lactic acid bacteria (LAB) were grown on Man Rogosa Sharpe (MRS) broth at 37 °C in anaerobic conditions. After that, the cell-free supernatant (CFS) were recovered to determine its antifungal activity by halo diffusion agar test. In addition, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) was determined for each L. plantarum CFS by 96-well microplates method. Additionally, CFS was used as a natural biocontrol agent on corn kernels and corn ears contaminated with Aspergillus flavus and Fusarium verticillioides, respectively. The L. plantarum CECT 749 CFS showed the highest antifungal effect against all essayed strains. Moreover, the employment of this CFS in food reduced the mycotoxin production at a percentage ranging from 73.7 to 99.7%. These results suggest that the L. plantarum CECT 749 CFS could be promising for the biocontrol of corn.

scholarly journals Effects of dietary fumonisin B1 on the onset of puberty, semen quality, fertility rates and testicular morphology in male rabbits

Reproduction ◽  
2010 ◽  
Vol 139 (2) ◽  
pp. 439-445 ◽  
Author(s):  
E O Ewuola ◽  
G N Egbunike
Keyword(s):  
Semen Quality ◽  
Fusarium Verticillioides ◽  
Sperm Concentration ◽  
Fumonisin B1 ◽  
Delayed Puberty ◽  
Fertility Rates ◽  
Embryo Survival ◽  
Embryo Mortality ◽  
Randomized Design ◽  
Testicular Morphology

The influences of dietary fumonisin B1(FB1), a metabolite ofFusarium verticillioides, on the onset of puberty, semen quality, fertility rates and testicular morphology in male rabbits (bucks) were studied. Forty male rabbits were randomly assigned and fed four diets containing 0.13, 5.0, 7.5 and 10.0 mg FB1/kg, constituting diets 1 (control), 2, 3 and 4 respectively, for a period of 175 days in a completely randomized design. During the last week of the feeding trial, two untreated female rabbits were mated to each of the four treated bucks per treatment to assess the fertility rate of the treated bucks. Onset of puberty in animals fed diets 3 and 4 was significantly (P<0.05) delayed by some 9–12 days. The weight at puberty, sperm concentration and total sperm/ejaculate were not significantly influenced by the dietary FB1. Sperm mass activities, motility and live spermatozoa of the rabbits' semen significantly (P<0.05) declined with an increase in the dietary FB1. The highest sperm cell abnormalities were recorded in the animals fed 10.0 mg/kg FB1, while the least was observed in the control animals. The conception rate, litter size and embryo survival rate were statistically the same among the dietary treatments. Embryo mortality was significantly (P<0.05) higher in rabbits fed diets 3 and 4 than in others. Testicular elements were significantly (P<0.05) impaired by the toxin in rabbits fed 7.5 and 10.0 mg FB1/kg. This suggests that LOAEL of 7.50 mg/kg FB1delayed puberty, impaired semen quality and spermatogenesis and induced embryo mortality without a statistically adverse effect on the fertility rates of male rabbits.

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