Expression profiles of OsNramp6 transcript variants involving in Magnaporthe oryzae resistance and non-resistance of Vietnamese rice cultivars

Rice blast, caused by the fungus Magnaporthe oryzae, is the most damaging disease for rice worldwide. However, the reactions of rice to M. oryzae at different growth stages are largely unknown. In the present study, two temperate japonica rice cultivars, M-202 and Nipponbare, were inoculated synchronously at different vegetative growth stages, V1 to V10. Plants of M-202 at each stage from V1 to reproductive stage R8 were inoculated with M. oryzae race (isolate) IB-49 (ZN61) under controlled conditions. Disease reactions were recorded 7 days postinoculation by measuring the percentage of diseased area of all leaves, excluding the youngest leaf. The results showed that the plants were significantly susceptible at the V1 to V4 stages with a disease severity of 26.7 to 46.8% and disease index of 18.62 to 37.76 for M-202. At the V1 to V2 stages, the plants were significantly susceptible with a disease a severity of 28.6 to 39.3% and disease index of 23.65 to 29.82 for Nipponbare. Similar results were observed when plants of M-202 were inoculated at each growth stage with a disease severity of 29.7 to 60.6% and disease index of 21.93 to 59.25 from V1 to V4. Susceptibility decreased after the V5 stage (severity 4.6% and index 2.17) and became completely resistant at the V9 to V10 stages and after the reproductive stages, suggesting that plants have enhanced disease resistance at later growth stages. These findings are useful for managing rice blast disease in commercial rice production worldwide.

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Expression of osa-miR7695 against the blast fungus Magnaporthe oryzae in Vietnamese rice cultivars

European Journal of Plant Pathology ◽

10.1007/s10658-019-01772-5 ◽

2019 ◽

Vol 155 (1) ◽

pp. 307-317 ◽

Cited By ~ 2

Author(s):

Nguyen Bao Quoc ◽

Nguyen Doan Nguyen Phuong ◽

Ho Thi Thu Trang ◽

Nguyen Bang Phi ◽

Nguyen Ngoc Bao Chau

Keyword(s):

Magnaporthe Oryzae ◽

Rice Cultivars ◽

Blast Fungus

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Expression of ZNF695 Transcript Variants in Childhood B-Cell Acute Lymphoblastic Leukemia

Genes ◽

10.3390/genes10090716 ◽

2019 ◽

Vol 10 (9) ◽

pp. 716 ◽

Cited By ~ 2

Author(s):

Rosa ◽

Villegas-Ruíz ◽

Caballero-Palacios ◽

Pérez-López ◽

Murata ◽

...

Keyword(s):

Acute Lymphoblastic Leukemia ◽

B Cell ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Expression Profiles ◽

Lymphoblastic Leukemia ◽

Alternative Transcript ◽

Human Transcriptome ◽

Transcript Variants ◽

Cell Acute Lymphoblastic Leukemia

B-cell acute lymphoblastic leukemia is the most commonly diagnosed childhood malignancy worldwide; more than 50% of these cases are diagnosed in Mexico. Although the five-year survival rate is >80%, 30% of patients experience relapse with poor prognosis. Cancer-associated gene expression profiles have been identified in several malignancies, and some transcripts have been used to predict disease prognosis. The human transcriptome is incompletely elucidated; moreover, more than 80% of transcripts can be processed via alternative splicing (AS), which increases transcript and protein diversity. The human transcriptome is divided; coding RNA accounts for 2%, and the remaining 98% is noncoding RNA. Noncoding RNA can undergo AS, promoting the diversity of noncoding transcripts. We designed specific primers to amplify previously reported alternative transcript variants of ZNF695 and showed that six ZNF695 transcript variants are co-expressed in cancer cell lines. The amplicons were sequenced and identified. Additionally, we analyzed the expression of these six transcript variants in bone marrow from B-cell acute lymphoblastic leukemia patients and observed that ZNF695 transcript variants one and three were the predominant variants expressed in leukemia. Moreover, our results showed the co-expression of coding and long noncoding RNA. Finally, we observed that long noncoding RNA ZNF695 expression predicted survival rates.

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Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa

Phytopathology ◽

10.1094/phyto-12-16-0421-r ◽

2017 ◽

Vol 107 (9) ◽

pp. 1039-1046 ◽

Cited By ~ 4

Author(s):

Emmanuel M. Mgonja ◽

Chan Ho Park ◽

Houxiang Kang ◽

Elias G. Balimponya ◽

Stephen Opiyo ◽

...

Keyword(s):

Genetic Diversity ◽

Association Mapping ◽

Magnaporthe Oryzae ◽

Rice Blast ◽

Blast Resistance ◽

Genotyping By Sequencing ◽

Rice Breeding ◽

Rice Cultivars ◽

R Genes ◽

African Rice

Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa is rice blast, caused by the hemibiotrophic fungal pathogen Magnaporthe oryzae. In this report, we present the results of a genotyping-by-sequencing (GBS)-based diversity analysis of 190 African rice cultivars and an association mapping of blast resistance (R) genes and quantitative trait loci (QTLs). The 190 African cultivars were clustered into three groups based on the 184K single nucleotide polymorphisms generated by GBS. We inoculated the rice cultivars with six African M. oryzae isolates. Association mapping identified 25 genomic regions associated with blast resistance (RABRs) in the rice genome. Moreover, PCR analysis indicated that RABR_23 is associated with the Pi-ta gene on chromosome 12. Our study demonstrates that the combination of GBS-based genetic diversity population analysis and association mapping is effective in identifying rice blast R genes/QTLs that contribute to resistance against African populations of M. oryzae. The identified markers linked to the RABRs and 14 highly resistant cultivars in this study will be useful for rice breeding in Africa.

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Transcript variants and expression profiles analysis of Mitf gene in minipigs

Journal of Otology ◽

10.1016/j.joto.2015.09.005 ◽

2015 ◽

Vol 10 (2) ◽

pp. 83-86

Author(s):

Weiwei Guo ◽

Lili Ren ◽

Lei Chen ◽

Yu Ning ◽

Lidong Zhao ◽

...

Keyword(s):

Expression Profiles ◽

Transcript Variants

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Germin Like Protein Genes Exhibit Modular Expression During Salt and Drought Stress in Elite Rice Cultivars

10.21203/rs.3.rs-713600/v1 ◽

2021 ◽

Author(s):

Jazba Anum ◽

Charlotte O’Shea ◽

M Zeeshan Hyder ◽

Sumaira Farrukh ◽

Karen Skriver ◽

...

Keyword(s):

Salt Stress ◽

Drought Stress ◽

Expression Profiles ◽

Rice Variety ◽

Chromosome 8 ◽

Tissue Type ◽

Rice Cultivars ◽

Relative Expression ◽

Salt And Drought Stress ◽

Leaves And Roots

Abstract Germin-like proteins (GLPs) are ubiquitous plant proteins, which play significant role in plant responses against various abiotic stresses. However, the potential functions of GLPs in rice (Oryza Sativa) against salt and drought stress are still unclear. In this study, transcriptional variation of 8 OsGLP genes (OsGLP3-6, OsGLP4-1, OsGLP8-4, OsGLP8-7, OsGLP8-10, OsGLP8-11 and OsGLP8-12) was analyzed in leaves and roots of two economically important Indica rice cultivars, KS282 and Super Basmati under salt and drought stress at early seedling stage. The relative expression analysis from qRT-PCR indicated the highest increase in expression of OsGLP3-6 in leaves and roots of both rice varieties with a significantly higher expression in KS282. Moreover, relative change in expression of OsGLP8-7, OsGLP8-10 and OsGLP8-11 under salt stress and OsGLP8-7 under drought stress was also commonly higher in leaves and roots of KS282 as compared to Super Basmati. Whereas, OsGLP3-7 and OsGLP8-12 after salt stress and OsGLP8-4 and OsGLP8-12 after drought stress were observed with higher relative expression in roots of Super Basmati than KS282. Importantly, the OsGLP3-6 and OsGLP4-1 from chromosome 3 and 4 respectively showed higher expression in leaves whereas most of the OsGLP genes from chromosome 8 exhibited higher expression in roots. Overall, as a result of this comparative analysis, OsGLP genes showed both general and specific expression profiles depending upon a specific rice variety, stress condition as well as tissue type. These results will increase our understanding of role of OsGLP genes in rice crop and provide useful information for the further in-depth research on their regulatory mechanisms in response to these stress conditions.

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Identification and characterization of 19 predicted Myb-family DNA binding proteins in Magnaporthe oryzae concerning growth, conidiation, and pathogenicity

10.1101/2021.12.28.474317 ◽

2021 ◽

Author(s):

Ya Li ◽

Xiuxia Zheng ◽

Mengtian Pei ◽

Mengting Chen ◽

Shengnan Zhang ◽

...

Keyword(s):

Stress Response ◽

Dna Binding ◽

Magnaporthe Oryzae ◽

Expression Profiles ◽

Dna Binding Proteins ◽

The Other ◽

Genes Encoding ◽

Identification And Characterization ◽

Different Levels

Genes encoding for proteins containing the DNA binding Myb domain have been suggested to be important in regulating development and stress response in eukaryotes, including fungi. Magnaporthe oryzae (teleomorph Pyricularia oryzae) is considered the most destructive pathogen of rice. We screen the M. oryzae genome for all genes encoding proteins containing Myb domains since these genes could be essential during pathogenesis. We found 19 genes Myb1-19. Only a few have previously been investigated, and only one has proven to be involved in pathogenesis. We tried to delete the other 18 genes and succeeded with all except 6, five of which could be essential. RT-qPCR showed that all 19 genes are expressed during pathogenesis, although at different levels and with different expression profiles. To our surprise, only deletions of the genes encoding proteins MoMyb2, MoMyb13, and MoMyb15 showed growth, conidiation, and infection phenotypes, indicating that they are essential on their own during infection. This lack of phenotypes for the other mutants surprised us, and we extended the analysis to look for expression co-regulation and found 5 co-regulated groups of predicted proteins with Myb-domains. We point to likely compensatory regulations of the other Myb-family genes hiding the effect of many deletions. Further studies of the Myb-family genes are thus of interest since revealing the functions of these genes with a possible effect on pathogenicity since these could be targets for future measures to control M. oryzae in rice.

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Carolina Foxtail (Alopecurus carolinianus): Susceptibility and Suitability as an Alternative Host to Rice Blast Disease (Magnaporthe oryzae [formerly M. grisea])

Plant Disease ◽

10.1094/pdis-92-4-0504 ◽

2008 ◽

Vol 92 (4) ◽

pp. 504-507 ◽

Cited By ~ 1

Author(s):

Y. Jia ◽

D. Gealy ◽

M. J. Lin ◽

L. Wu ◽

H. Black

Keyword(s):

Magnaporthe Oryzae ◽

Rice Blast ◽

Rice Blast Disease ◽

Blast Disease ◽

Artificial Inoculation ◽

Rice Cultivars ◽

Alternative Host ◽

Lesion Development ◽

Disease Symptoms ◽

Rice Leaves

Carolina foxtail (Alopecurus carolinianus) has not been reported to host Magnaporthe oryzae. A collection of Carolina foxtail obtained from several Arkansas locations over a 4-year period was inoculated with four races of the fungus under greenhouse conditions and, in all cases, inoculation resulted in the formation of irregular, yellow and brown lesions without obvious gray centers that are characteristic for blast on rice. Differences in these lesions were not observed among our collection. These lesions appeared to differ from typical blast lesions on inoculated rice leaves but were evident following artificial inoculation of Carolina foxtail in the greenhouse. M. oryzae races that differed in pathogenicity toward rice cultivars also displayed differences in lesion development on Carolina foxtail. The most virulent race on rice cultivars also produced lesions most rapidly on Carolina foxtail. These lesions developed more quickly on Carolina foxtail than on the most susceptible rice cultivars tested, including a susceptible California cultivar, M202. M. oryzae isolates cultured from these lesions in the infected Carolina foxtail caused typical disease symptoms of blast on inoculated rice cultivars. We suggest that Carolina foxtail is a new and previously unrecognized host for the blast pathogen.

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