Comparison of polymerase chain reaction and standard microbiological techniques in presumed bacterial corneal ulcers

2014 ◽  
Vol 35 (2) ◽  
pp. 159-165 ◽  
Author(s):  
Anita Panda ◽  
Tajinder Pal Singh ◽  
Geeta Satpathy ◽  
Meenakshi Wadhwani ◽  
Monika Matwani
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Corneal Ulcers ◽  
Microbiological Techniques ◽  
Polymerase Chain

Detection of Pectinatus Beer Spoilage Bacteria by Using the Polymerase Chain Reaction

1997 ◽  
Vol 60 (12) ◽  
pp. 1571-1573 ◽  
Author(s):  
REETTA SATOKARI ◽  
RIIKKA JUVONEN ◽  
ATTE VON WRIGHT ◽  
AULI HAIKARA
Keyword(s):  
Polymerase Chain Reaction ◽  
Control Measure ◽  
Anaerobic Bacteria ◽  
Reaction Products ◽  
Chain Reaction ◽  
Spoilage Bacteria ◽  
Microbiological Techniques ◽  
Beer Spoilage ◽  
Polymerase Chain ◽  
Product Control

Anaerobic bacteria of the genus Pectinatus cause beer spoilage by producing off flavors and turbidity. In unpasteurized beer even a small initial amount of contamination is likely to lead to a defective product. Detection of Pectinatus by traditional microbiological techniques is time-consuming and not practical as a preventive product control measure. In this paper Pectinatus-specific primers capable of discriminating among other beer contaminants by polymerase chain reaction are described. The present procedure, which includes the isolation of DNA from the contaminated beer sample, the polymerase chain reaction, and the electrophoretic identification of the reaction products could be performed within 10 h. The detection level in inoculated beer samples was ca. 20 cells per ml. The technique therefore has a potential in routine product control.

scholarly journals Isolation and identification of Enteropathogenic Escherichia coli (EPEC) in paediatric diarrhoeal patients by detection of bfpA gene by PCR

2013 ◽  
Vol 7 (2) ◽  
pp. 02-05
Author(s):  
Shimu Saha ◽  
Sanya Tahmina Jhora ◽  
Shikha Paul ◽  
Israt Jahan Azmi ◽  
Tarek Mahbub Khan
Keyword(s):  
Polymerase Chain Reaction ◽  
Tertiary Care ◽  
Diarrhoeal Disease ◽  
Care Hospital ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Two Samples ◽  
Microbiological Techniques ◽  
Polymerase Chain

This study has been undertaken to investigate the isolation and identification of EPEC strains from paediatric diarrhoeal patients.Total 300 samples were studied.Two hundred and seventy two samples from patients with diarrhoea and  28 samples from control children were collected from two tertiary care hospital. Esch. coli was isolated and identified from all the 300 samples including patient and control using standard microbiological techniques. EPEC strains were identified on the basis of  presence of bundle forming pilus (bfpA) gene. Out of 272 samples from diarrhoeal patient only Esch. coli was isolated from 240 (88.2%) samples. Shigella spp. with Esch. coli were isolated from 27 (10%) specimens and Salmonella spp. with Esch. coli were isolated from 5 (1.8%) samples. Among 272 samples 20 (7.35%) isolates were identified as EPEC on the basis of presence of bfpA gene detected by polymerase chain reaction. EPEC strains were identified from those 240 samples, from which Esch. coli had been isolated only. No EPEC strain was identified  from control children. Rapid and reliable detection of EPEC is required for successful microbiological surveillance and for treatment of EPEC mediated diarrhoeal disease. bfpA gene detection by polymerase chain reaction can be a appropriate method where facilities for polymerase chain reaction are available.DOI: http://dx.doi.org/10.3329/bjmm.v7i2.19324 Bangladesh J Med Microbiol 2013; 07(02): 2-5

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of the Glanzmann's Thrombasthenia Mutations in Arab and Iraqi-Jewish Patients by Polymerase Chain Reaction and Restriction Analysis of Blood or Urine Samples

1991 ◽  
Vol 66 (04) ◽  
pp. 500-504 ◽  
Author(s):  
H Peretz ◽  
U Seligsohn ◽  
E Zwang ◽  
B S Coller ◽  
P J Newman
Keyword(s):  
Polymerase Chain Reaction ◽  
Base Pair ◽  
Restriction Analysis ◽  
Urine Samples ◽  
Chain Reaction ◽  
Base Pairs ◽  
Glanzmann’S Thrombasthenia ◽  
Glanzmann's Thrombasthenia ◽  
Base Pair Deletion ◽  
Polymerase Chain

SummarySevere Glanzmann's thrombasthenia is relatively frequent in Iraqi-Jews and Arabs residing in Israel. We have recently described the mutations responsible for the disease in Iraqi-Jews – an 11 base pair deletion in exon 12 of the glycoprotein IIIa gene, and in Arabs – a 13 base pair deletion at the AG acceptor splice site of exon 4 on the glycoprotein IIb gene. In this communication we show that the Iraqi-Jewish mutation can be identified directly by polymerase chain reaction and gel electrophoresis. With specially designed oligonucleotide primers encompassing the mutation site, an 80 base pair segment amplified in healthy controls was clearly distinguished from the 69 base pair segment produced in patients. Patients from 11 unrelated Iraqi-Jewish families had the same mutation. The Arab mutation was identified by first amplifying a DNA segment consisting of 312 base pairs in controls and of 299 base pairs in patients, and then digestion by a restriction enzyme Stu-1, which recognizes a site that is absent in the mutant gene. In controls the 312 bp segment was digested into 235 and 77 bp fragments, while in patients there was no change in the size of the amplified 299 bp segment. The mutation was found in patients from 3 out of 5 unrelated Arab families. Both Iraqi-Jewish and Arab mutations were detectable in DNA extracted from blood and urine samples. The described simple methods of identifying the mutations should be useful for detection of the numerous potential carriers among the affected kindreds and for prenatal diagnosis using DNA extracted from chorionic villi samples.

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