BODIPY vital staining as a tool for flow cytometric monitoring of intracellular lipid accumulation in Nannochloropsis gaditana

2014 ◽  
Vol 27 (1) ◽  
pp. 233-241 ◽  
Author(s):  
Vanessa Benito ◽  
Felipe Goñi-de-Cerio ◽  
Pilar Brettes
Keyword(s):  
Lipid Accumulation ◽  
Vital Staining ◽  
Intracellular Lipid ◽  
Nannochloropsis Gaditana ◽  
Flow Cytometric ◽  
Intracellular Lipid Accumulation

Cell viability and intracellular lipid accumulation in HepG2 cells under the influence of oleic acid

2011 ◽  
Vol 44 (13) ◽  
pp. S79
Author(s):  
Ziamajidi Nasrin ◽  
Nowrouzi Azin ◽  
Khaghani Shahnaz ◽  
Abdi rad Afshin
Keyword(s):  
Oleic Acid ◽  
Cell Viability ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

scholarly journals Association of intracellular lipid accumulation in subcutaneous adipocyte precursors and plasma adipokines in bariatric surgery candidates

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Ioana Hristov ◽  
Veronica Mocanu ◽  
Florin Zugun-Eloae ◽  
Luminita Labusca ◽  
Iustina Cretu-Silivestru ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Lipid Accumulation ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

Dysregulation of low-density lipoprotein receptor contributes to podocyte injuries in diabetic nephropathy

2015 ◽  
Vol 308 (12) ◽  
pp. E1140-E1148 ◽  
Author(s):  
Yang Zhang ◽  
Kun Ling Ma ◽  
Jing Liu ◽  
Yu Wu ◽  
Ze Bo Hu ◽  
...  
Keyword(s):  
High Glucose ◽  
Lipid Accumulation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Intracellular Lipid ◽  
Phenotypic Alteration ◽  
Density Lipoprotein Receptor ◽  
Intracellular Lipid Accumulation

Dyslipidemia plays crucial roles in the progression of diabetic nephropathy (DN). This study investigated the effects of high glucose on lipid accumulation in podocytes and explored its underlying mechanisms. Male db/m and db/db mice were fed a normal chow diet for 8 wk. Immortalised mouse podocytes were treated with or without high glucose for 24 h. The changes to the morphology and ultramicrostructures of the kidneys in mice were examined using pathological staining and electron microscopy. Intracellular lipid accumulation was evaluated by Oil Red O staining and a free cholesterol quantitative assay. The expressions of the molecules involved in low-density lipoprotein receptor (LDLr) pathway and podocyte injury were examined using immunofluorescent staining, real-time PCR, and Western blot. There were increased levels of plasma lipid, serum creatinine, and proteinuria in db/db mice compared with db/m mice. Moreover, there was significant mesangial matrix expansion, basement membrane thickening, podocyte foot process effacement, and phenotypic alteration in the db/db group. Additionally, lipid accumulation in the kidneys of db/db mice was increased due to increased protein expressions of LDLr, sterol regulatory element-binding protein (SREBP) cleavage-activating protein, and SREBP-2. These effects were further confirmed by in vitro studies. Interestingly, the treatment with LDLr siRNA inhibited lipid accumulation in podocytes and decreased the protein expression of molecules associated with phenotypic alteration in podocytes. High glucose disrupted LDLr feedback regulation in podocytes, which may cause intracellular lipid accumulation and alteration of podocyte phenotype, thereby accelerating DN progression.

scholarly journals CCAAT/enhancer-binding protein delta promotes intracellular lipid accumulation in M1 macrophages of vascular lesions

2017 ◽  
Vol 113 (11) ◽  
pp. 1376-1388 ◽  
Author(s):  
Hong-Yue Lai ◽  
Ling-Wei Hsu ◽  
Hsin-Hwa Tsai ◽  
Yu-Chih Lo ◽  
Shang-Hsun Yang ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Binding Protein ◽  
Vascular Lesions ◽  
Intracellular Lipid ◽  
M1 Macrophages ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Intracellular Lipid Accumulation

scholarly journals Zanthoxylum ailanthoides Suppresses Oleic Acid-Induced Lipid Accumulation through an Activation of LKB1/AMPK Pathway in HepG2 Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Eun-Bin Kwon ◽  
Myung-Ji Kang ◽  
Soo-Yeon Kim ◽  
Yong-Moon Lee ◽  
Mi-Kyeong Lee ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Methanol Extract ◽  
De Novo ◽  
Oxygen Species ◽  
Gene Expressions ◽  
Intracellular Lipid ◽  
Ampk Signaling ◽  
Intracellular Lipid Accumulation

Zanthoxylum ailanthoides (ZA) has been used as folk medicines in East Asian and recently reported to have several bioactivity; however, the studies of ZA on the regulation of triacylglycerol (TG) biosynthesis have not been elucidated yet. In this study, we examined whether the methanol extract of ZA (ZA-M) could reduce oleic acid- (OA-) induced intracellular lipid accumulation and confirmed its mode of action in HepG2 cells. ZA-M was shown to promote the phosphorylation of AMPK and its upstream LKB1, followed by reduction of lipogenic gene expressions. As a result, treatment of ZA-M blocked de novo TG biosynthesis and subsequently mitigated intracellular neutral lipid accumulation in HepG2 cells. ZA-M also inhibited OA-induced production of reactive oxygen species (ROS) and TNF-α, suggesting that ZA-M possess the anti-inflammatory feature in fatty acid over accumulated condition. Taken together, these results suggest that ZA-M attenuates OA-induced lipid accumulation and inflammation through the activation of LKB1/AMPK signaling pathway in HepG2 cells.

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