scholarly journals Quercetin modified electrospun PHBV fibrous scaffold enhances cartilage regeneration

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
Wei Chen ◽  
Yongsheng Li ◽  
Yuting Huang ◽  
Yao Dai ◽  
Tingfei Xi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Fibrous Scaffold ◽  
Fibrous Scaffolds ◽  
Modification Method ◽  
Hydroxyvaleric Acid ◽  
Chondrocytic Phenotype

AbstractIt suggests that the poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) scaffold can be used for cartilage tissue engineering, but PHBV is short of bioactivity that is required for cartilage regeneration. To fabricate a bioactive cartilage tissue engineering scaffold that promotes cartilage regeneration, quercetin (QUE) modified PHBV (PHBV-g-QUE) fibrous scaffolds were prepared by a two-step surface modification method. The PHBV-g-QUE fibrous scaffold facilitates the growth of chondrocytes and maintains chondrocytic phenotype resulting from the upregulation of SOX9, COL II, and ACAN. The PHBV-g-QUE fibrous scaffold inhibited apoptosis of chondrocyte and reduced oxidative stress of chondrocytes by regulating the transcription of related genes. Following PHBV-g-QUE fibrous scaffolds and PHBV fibrous scaffolds with adhered chondrocytes were implanted into nude mice for 4 weeks, it demonstrated that PHBV-g-QUE fibrous scaffolds significantly promoted cartilage regeneration compared with the PHBV fibrous scaffolds. Hence, it suggests that the PHBV-g-QUE fibrous scaffold can be potentially applied in the clinical treatment of cartilage defects in the future.

scholarly journals Articular cartilage tissue engineering with stem cells implanted onto nanoscaffolds and platelet-rich plasma

2017 ◽  
Vol 3 (3) ◽  
pp. 322
Author(s):  
Hadeer A. Abbassy ◽  
Laila M. Montaser ◽  
Sherin M. Fawzy
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Articular Cartilage ◽  
Soft Tissues ◽  
Platelet Rich Plasma ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Great Promise

<p class="abstract">Musculoskeletal medicine targets both cartilage regeneration and healing of soft tissues. Articular cartilage repair and regeneration is primarily considered to be due to its poor regenerative properties. Cartilage defects due to joint injury, aging, or osteoarthritis have low self-repair ability thus they are most often irreversible as well as being a major cause of joint pain and chronic disability. Unfortunately, current methods do not seamlessly restore hyaline cartilage and may lead to the formation of fibro- or continue hypertrophic cartilage. Deficiency of efficient modalities of therapy has invited research to combine stem cells, scaffold materials and environmental factors through tissue engineering. Articular cartilage tissue engineering aims to repair, regenerate, and hence improve the function of injured or diseased cartilage. This holds great potential and has evoked intense interest in improving cartilage therapy. Platelet-rich plasma (PRP) and/or stem cells may be influential for tissue repair as well as cartilage regenerative processes.  A great promise to advance current cartilage therapies toward achieving a consistently successful modality has been held for addressing cartilage afflictions. The use of stem cells, novel biologically inspired scaffolds and, emerging nanotechnology may be the best way to reach this objective via tissue engineering. A current and emergent approach in the field of cartilage tissue engineering is explained in this review for specific application. In the future, the development of new strategies using stem cells seeded in scaffolds and the culture medium supplemented with growth factors could improve the quality of the newly formed cartilage<span lang="EN-IN">.</span></p>

NANOSTRUCTURED SCAFFOLDS FOR BIOLOGY AND TISSUE ENGINEERING

Nano LIFE ◽  
2010 ◽  
Vol 01 (01n02) ◽  
pp. 109-120 ◽  
Author(s):  
JOHN G. BARBER ◽  
WAN-JU LI
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Biology ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cellular Responses ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Musculoskeletal Tissues ◽  
Materials Used

Cartilage defects remain one of the most challenging musculoskeletal tissues to treat owing to its poor healing capacity. The lack of sufficient clinical treatments has led to a drive in tissue engineering advancements that combine chondrogenic cells with scaffolds to aid in cartilage regeneration. Nanoscale materials are commonly used in scaffold synthesis because of their ability to mimic the size of extracellular matrix (ECM). This review focuses on the use of nanostructured scaffolds in combination with cells for cartilage tissue engineering. We detail the fabrication methods and materials used to produce nanostructured scaffolds, with a focus on nanofibers and their role in modulating cell biology. Lastly, we discuss various techniques that further functionalize the nanostructured scaffolds to enhance cellular responses.

Development of a Biomimetic Electrospun Microfibrous Scaffold With Multiwall Carbon Nanotubes for Cartilage Regeneration

2013 ◽  
Author(s):  
Benjamin Holmes ◽  
Nathan J. Castro ◽  
Jian Li ◽  
Lijie Grace Zhang
Keyword(s):  
Carbon Nanotubes ◽  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Multiwall Carbon Nanotubes ◽  
Cartilage Regeneration ◽  
Modern Medicine ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Medical Problems

Cartilage defects, which are caused by a variety of reasons such as traumatic injuries, osteoarthritis, or osteoporosis, represent common and severe clinical problems. Each year, over 6 million people visit hospitals in the U.S. for various knee, wrist, and ankle problems. As modern medicine advances, new and novel methodologies have been explored and developed in order to solve and improve current medical problems. One of the areas of investigation that has thus far proven to be very promising is tissue engineering [1, 2]. Since cartilage matrix is nanocomposite, the goal of the current work is to use nanomaterials and nanofabrication methods to create novel biologically inspired tissue engineered cartilage scaffolds for facilitating human bone marrow mesenchymal stem cell (MSC) chondrogenesis. For this purpose, through electrospinning techniques, we designed a series of novel 3D biomimetic nanostructured scaffolds based on carbon nanotubes and biocompatible poly(L-lactic acid) (PLLA) polymers. Specifically, a series of electrospun fibrous PLLA scaffolds with controlled fiber dimension were fabricated in this study. In vitro hMSC studies showed that stem cells prefer to attach in the scaffolds with smaller fiber diameter. More importantly, our in vitro differentiation results demonstrated that incorporation of the biomimetic carbon nanotubes and poly L-lysine coating can induce more chondrogenic differentiations of MSCs than controls, which make them promising for cartilage tissue engineering applications.

scholarly journals A Review of the Use of Microparticles for Cartilage Tissue Engineering

2021 ◽  
Vol 22 (19) ◽  
pp. 10292
Author(s):  
Rachel J. Kulchar ◽  
Bridget R. Denzer ◽  
Bharvi M. Chavre ◽  
Mina Takegami ◽  
Jennifer Patterson
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Joint Space ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Enabling Technology ◽  
Cell Therapies ◽  
The World

Tissue and organ failure has induced immense economic and healthcare concerns across the world. Tissue engineering is an interdisciplinary biomedical approach which aims to address the issues intrinsic to organ donation by providing an alternative strategy to tissue and organ transplantation. This review is specifically focused on cartilage tissue. Cartilage defects cannot readily regenerate, and thus research into tissue engineering approaches is relevant as a potential treatment option. Cells, scaffolds, and growth factors are three components that can be utilized to regenerate new tissue, and in particular recent advances in microparticle technology have excellent potential to revolutionize cartilage tissue regeneration. First, microspheres can be used for drug delivery by injecting them into the cartilage tissue or joint space to reduce pain and stimulate regeneration. They can also be used as controlled release systems within tissue engineering constructs. Additionally, microcarriers can act as a surface for stem cells or chondrocytes to adhere to and expand, generating large amounts of cells, which are necessary for clinically relevant cell therapies. Finally, a newer application of microparticles is to form them together into granular hydrogels to act as scaffolds for tissue engineering or to use in bioprinting. Tissue engineering has the potential to revolutionize the space of cartilage regeneration, but additional research is needed to allow for clinical translation. Microparticles are a key enabling technology in this regard.

scholarly journals Digital Light Processing Bioprinted Human Chondrocyte-Laden Poly (γ-Glutamic Acid)/Hyaluronic Acid Bio-Ink towards Cartilage Tissue Engineering

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 714
Author(s):  
Alvin Kai-Xing Lee ◽  
Yen-Hong Lin ◽  
Chun-Hao Tsai ◽  
Wan-Ting Chang ◽  
Tsung-Li Lin ◽  
...  
Keyword(s):  
United States ◽  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Glutamic Acid ◽  
Cellular Proliferation ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
The United States ◽  
Cartilage Injury ◽  
Cartilage Tissue

Cartilage injury is the main cause of disability in the United States, and it has been projected that cartilage injury caused by osteoarthritis will affect 30% of the entire United States population by the year 2030. In this study, we modified hyaluronic acid (HA) with γ-poly(glutamic) acid (γ-PGA), both of which are common biomaterials used in cartilage engineering, in an attempt to evaluate them for their potential in promoting cartilage regeneration. As seen from the results, γ-PGA-GMA and HA, with glycidyl methacrylate (GMA) as the photo-crosslinker, could be successfully fabricated while retaining the structural characteristics of γ-PGA and HA. In addition, the storage moduli and loss moduli of the hydrogels were consistent throughout the curing durations. However, it was noted that the modification enhanced the mechanical properties, the swelling equilibrium rate, and cellular proliferation, and significantly improved secretion of cartilage regeneration-related proteins such as glycosaminoglycan (GAG) and type II collagen (Col II). The cartilage tissue proof with Alcian blue further demonstrated that the modification of γ-PGA with HA exhibited suitability for cartilage tissue regeneration and displayed potential for future cartilage tissue engineering applications. This study built on the previous works involving HA and further showed that there are unlimited ways to modify various biomaterials in order to further bring cartilage tissue engineering to the next level.

scholarly journals Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

Polymers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 4199
Author(s):  
Mahshid Hafezi ◽  
Saied Nouri Khorasani ◽  
Mohadeseh Zare ◽  
Rasoul Esmaeely Neisiany ◽  
Pooya Davoodi
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Biomechanical Properties ◽  
Tissue Growth ◽  
Cartilage Tissue ◽  
Self Healing ◽  
Advantages And Disadvantages ◽  
Wide Range

Cartilage is a tension- and load-bearing tissue and has a limited capacity for intrinsic self-healing. While microfracture and arthroplasty are the conventional methods for cartilage repair, these methods are unable to completely heal the damaged tissue. The need to overcome the restrictions of these therapies for cartilage regeneration has expanded the field of cartilage tissue engineering (CTE), in which novel engineering and biological approaches are introduced to accelerate the development of new biomimetic cartilage to replace the injured tissue. Until now, a wide range of hydrogels and cell sources have been employed for CTE to either recapitulate microenvironmental cues during a new tissue growth or to compel the recovery of cartilaginous structures via manipulating biochemical and biomechanical properties of the original tissue. Towards modifying current cartilage treatments, advanced hydrogels have been designed and synthesized in recent years to improve network crosslinking and self-recovery of implanted scaffolds after damage in vivo. This review focused on the recent advances in CTE, especially self-healing hydrogels. The article firstly presents the cartilage tissue, its defects, and treatments. Subsequently, introduces CTE and summarizes the polymeric hydrogels and their advances. Furthermore, characterizations, the advantages, and disadvantages of advanced hydrogels such as multi-materials, IPNs, nanomaterials, and supramolecular are discussed. Afterward, the self-healing hydrogels in CTE, mechanisms, and the physical and chemical methods for the synthesis of such hydrogels for improving the reformation of CTE are introduced. The article then briefly describes the fabrication methods in CTE. Finally, this review presents a conclusion of prevalent challenges and future outlooks for self-healing hydrogels in CTE applications.

scholarly journals Numerical Simulation of Electroactive Hydrogels for Cartilage–Tissue Engineering

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 2913 ◽  
Author(s):  
Abdul Razzaq Farooqi ◽  
Julius Zimmermann ◽  
Rainer Bader ◽  
Ursula van Rienen
Keyword(s):  
Tissue Engineering ◽  
Electric Stimulation ◽  
Mathematical Formulation ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Hydrogel Scaffolds ◽  
Finite Element Software ◽  
Defect Site ◽  
Articular Cartilage Defects

The intrinsic regeneration potential of hyaline cartilage is highly limited due to the absence of blood vessels, lymphatics, and nerves, as well as a low cell turnover within the tissue. Despite various advancements in the field of regenerative medicine, it remains a challenge to remedy articular cartilage defects resulting from trauma, aging, or osteoarthritis. Among various approaches, tissue engineering using tailored electroactive scaffolds has evolved as a promising strategy to repair damaged cartilage tissue. In this approach, hydrogel scaffolds are used as artificial extracellular matrices, and electric stimulation is applied to facilitate proliferation, differentiation, and cell growth at the defect site. In this regard, we present a simulation model of electroactive hydrogels to be used for cartilage–tissue engineering employing open-source finite-element software FEniCS together with a Python interface. The proposed mathematical formulation was first validated with an example from the literature. Then, we computed the effect of electric stimulation on a circular hydrogel sample that served as a model for a cartilage-repair implant.

Treatment of articular cartilage defects in horses with polymer-based cartilage tissue engineering grafts

Biomaterials ◽  
2006 ◽  
Vol 27 (14) ◽  
pp. 2882-2889 ◽  
Author(s):  
Dirk Barnewitz ◽  
Michaela Endres ◽  
Ina Krüger ◽  
Anja Becker ◽  
Jürgen Zimmermann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Articular Cartilage Defects

Methylsulfonylmethane-loaded electrospun poly(lactide-co-glycolide) mats for cartilage tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (117) ◽  
pp. 96725-96732 ◽  
Author(s):  
Zongliang Wang ◽  
Yu Wang ◽  
Peibiao Zhang ◽  
Xuesi Chen
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Promising Candidate

The electrospun MSM-loaded PLGA mat is a promising candidate for cartilage regeneration.

scholarly journals Chondrogenic Potential of Dental-Derived Mesenchymal Stromal Cells

Osteology ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 149-174
Author(s):  
Naveen Jeyaraman ◽  
Gollahalli Shivashankar Prajwal ◽  
Madhan Jeyaraman ◽  
Sathish Muthu ◽  
Manish Khanna
Keyword(s):  
Tissue Engineering ◽  
Mesenchymal Stromal Cells ◽  
Tissue Regeneration ◽  
Stromal Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Dentin Matrix

The field of tissue engineering has revolutionized the world in organ and tissue regeneration. With the robust research among regenerative medicine experts and researchers, the plausibility of regenerating cartilage has come into the limelight. For cartilage tissue engineering, orthopedic surgeons and orthobiologists use the mesenchymal stromal cells (MSCs) of various origins along with the cytokines, growth factors, and scaffolds. The least utilized MSCs are of dental origin, which are the richest sources of stromal and progenitor cells. There is a paradigm shift towards the utilization of dental source MSCs in chondrogenesis and cartilage regeneration. Dental-derived MSCs possess similar phenotypes and genotypes like other sources of MSCs along with specific markers such as dentin matrix acidic phosphoprotein (DMP) -1, dentin sialophosphoprotein (DSPP), alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP), and STRO-1. Concerning chondrogenicity, there is literature with marginal use of dental-derived MSCs. Various studies provide evidence for in-vitro and in-vivo chondrogenesis by dental-derived MSCs. With such evidence, clinical trials must be taken up to support or refute the evidence for regenerating cartilage tissues by dental-derived MSCs. This article highlights the significance of dental-derived MSCs for cartilage tissue regeneration.

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