scholarly journals Heat damage of cytoskeleton in erythrocytes increases membrane roughness and cell rigidity

2019 ◽  
Vol 45 (4) ◽  
pp. 367-377
Author(s):  
E. Yu Parshina ◽  
A. I. Yusipovich ◽  
A. R. Brazhe ◽  
M. A. Silicheva ◽  
G. V. Maksimov
Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.


Nature ◽  
1946 ◽  
Vol 158 (4004) ◽  
pp. 120-121 ◽  
Author(s):  
J. B. HUTCHINSON ◽  
E. N. GREER ◽  
P. T. THOMAS
Keyword(s):  

1986 ◽  
Vol 66 (3) ◽  
pp. 743-753 ◽  
Author(s):  
A. S. ATWAL ◽  
L. C. HESLOP ◽  
K. LIEVERS

Two experiments were conducted in sequence to determine the effectiveness of anhydrous ammonia (AA) as a preservative of alfalfa hay stored as large round bales (LRB). In the the first experiment application of 3% AA (wt/wt) to alfalfa hay baled at about 23% moisture-content (MC) increased the temperature markedly above the control treatment and resulted in a significantly (P < 0.05) higher acid-detergent-insoluble nitrogen and acid-detergent lignin. In the second experiment application of about 1% AA slowly (over 48 h) to LRB of alfalfa hay packaged at about 30% and > 35% MC, significantly (P < 0.05) reduced the cumulative degree-days above all reference points from 35 to 65 °C. Ammoniation completely eliminated dry matter losses in storage which were highest (8.3%) for 33% MC control hay. High moisture control hay (33% MC) suffered severe heat damage with 33% of total N being in the acid-detergent-insoluble fraction as compared to less than 10% for medium-moisture (26% MC) control and even lower values for ammoniated hay. Digestibility of crude protein and energy was significantly (P < 0.05) improved by ammoniation of medium-moisture (about 30% in-to-storage) alfalfa hay. When the ammonia-treated hay was uncovered in spring, coarsely ground and stored in a hay wagon the high-moisture-ammoniated hay (> 35% MC) became moldy within 4 wk, even when the ambient temperature was about 6 °C, whereas medium-moisture-ammoniated hay (about 30% MC) showed some deterioration after 7 wk as the ambient temperature increased to about 12 °C. Key words: Ammonia, alfalfa hay, digestibility, heat damage, high-moisture hay, large bales


2021 ◽  
Vol 18 (1) ◽  
pp. 7-14
Author(s):  
Irina Volkova ◽  
Kseniia Pitulko ◽  
Anzhelika Sergeeva ◽  
Saida Pshidatok

This work aims to study the effect of high temperatures and phytopathogenic bacteria on different types of trees in the forest shelterbelts. Therefore, in 2018, 17 tree species were studied from 50 sample sites in the Moscow oblast (Russia). Leaf scorching, diseases caused by phytopathogenic bacteria, and heat damage to the crowns were examined in 5224 tree species. Based on the degree of crown damage, the studied tree species were divided into four classes. It was found that the heat damage to tree crowns was identical between the three sampling aspects (correlation coefficient 0.99). The plant species composition must be considered when developing forest shelterbelts. A long-term forecast on structural changes of planted areas is possible, considering the species composition and climatic characteristics of the region. Class 5 includes only chestnut; class 4 includes three species; class 3 is represented by seven species. Class 2, includes six species, and is the most suitable in developing forest plantations. No tree species in class 1 were found (trees with no damage). There is a connection between pathologies and heat injuries in trees from classes 4 to 5 (correlation 0.89).


1999 ◽  
Author(s):  
D. Shan ◽  
H. Nayeb-Hashemi

Abstract Aluminum alloys reinforced with B4C particle of average particle size of 10.5 μm and 20.9 μm were exposed to 535°C for various duration. The material degradation was evaluated by both mechanical and acoustic emission monitoring during tensile test. The micromechanisms of fracture changed from particle fracture and debonding, for as received specimens, to particle/matrix debonding after exposure to high temperature. The cumulative acoustic emission (AE) events during post heat exposure reduced with the increase of heal damage on the specimen. A Weibull probability distribution function that incorporates heat damage and relates to the cumulative events is developed. This model could be used to evaluate the extent of heat damage to a composite specimen.


1966 ◽  
Vol 151 (6) ◽  
pp. 652-654 ◽  
Author(s):  
J.J. Vos
Keyword(s):  

2007 ◽  
Vol 227 (1) ◽  
pp. 117-124 ◽  
Author(s):  
José Contreras-Calderón ◽  
Eduardo Guerra-Hernández ◽  
Belén García-Villanova

2011 ◽  
Vol 396-398 ◽  
pp. 516-519
Author(s):  
Yi Zhang ◽  
Dong Ming Guo ◽  
Li Meng

With the deep mining in coal mine, heat damage is one of the technical issues need to be solved. HEMS cooling system in Sanhejian Coal Mine is a process system for high-temperature heat damage controlling in deep coal mine, in which cool energy extracted to reduce work face’s ambient temperature to achieve heat damage controlling. Part of the cool energy is from the level circulating of cooling water in -700 level main raodway, the other is from the mine water. We analyze the energy consumption of every subsystem during operation of the HEMS system, which could provide a theoretical basis and technical guidance on more efficiently running of cooling system deep in the future.


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