scholarly journals Serum Sp17 Autoantibody Serves as a Potential Specific Biomarker in Patients with SAPHO Syndrome

2021 ◽  
Author(s):  
Hongqin You ◽  
Guanglei Dang ◽  
Bichao Lu ◽  
Siya Zhang ◽  
Chen Li ◽  
...  
Keyword(s):  
Disease Status ◽  
Serum Levels ◽  
Sapho Syndrome ◽  
Inactive Disease ◽  
C Reactive Protein ◽  
Cutaneous Manifestations ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Wide Variability ◽  
Pamidronate Disodium

AbstractSAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome shows a wide variability in musculoskeletal and cutaneous manifestations, and it is therefore underrecognized and misdiagnosed in the clinic due to a lack of specific markers. In this study, we aimed to identify specific biomarkers by screening serum autoantibodies in SAPHO patients with a 17K human whole-proteome microarray. The serum anti-Sp17 autoantibody was identified and verified to be a specific biomarker in patients with SAPHO syndrome. Indeed, the level of the anti-Sp17 autoantibody was significantly increased in patients with active SAPHO compared to patients with an inactive disease and healthy controls (P < 0.05). Additionally, serum anti-Sp17 autoantibody levels correlated with those of serum hypersensitive C-reactive protein (hsCRP), the erythrocyte sedimentation rate (ESR), and β-crosslaps (β-CTx) in patients with active SAPHO disease. Moreover, anti-Sp17 autoantibody levels were markedly decreased after anti-inflammatory treatment with pamidronate disodium, which downregulated levels of hsCRP and ESR in patients with active SAPHO. Thus, serum levels of the anti-Sp17 autoantibody might serve as a specific biomarker for the diagnosis of SAPHO syndrome or for monitoring the disease status.

scholarly journals Expression of ICAM1 and VCAM1 Serum Levels in Rheumatoid Arthritis Clinical Activity. Association with Genetic Polymorphisms

2009 ◽  
Vol 26 (3) ◽  
pp. 119-126 ◽  
Author(s):  
Rosa Elena Navarro-Hernández ◽  
Edith Oregon-Romero ◽  
Mónica Vázquez-Del Mercado ◽  
Héctor Rangel-Villalobos ◽  
Claudia Azucena Palafox-Sánchez ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Clinical Status ◽  
Serum Levels ◽  
Clinical Activity ◽  
C Reactive Protein ◽  
Soluble Adhesion Molecules ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Pcr Rflp ◽  
Elisa Technique

To investigate the association of sICAM-1 and sVCAM-1 with ICAM1 721G>A and VCAM1 1238G>C polymorphisms and rheumatoid arthritis (RA) clinical activity, sixty RA patients and 60 healthy non-related subjects (HS) matched for age and sex were recruited. Soluble adhesion molecules were determined by ELISA technique. Rheumatoid factor (RF), C reactive protein (CRP) and the erythrocyte sedimentation rate (ESR) were measured by routine methods. Disability and clinical activity was measured with Spanish-HAQ-DI and DAS28 scores, respectively. The ICAM1 and VCAM1 polymorphism were identified using the PCR-RFLP procedure. Inter-group comparison showed increased levels of sICAM-1 and sVCAM-1 in RA patients (284 and 481 ng/mL) versus HS (132 and 280 ng/mL); in the RA group, significant correlations between sVCAM-1 and RF (r= 0.402), ESR (r= 0.426), Spanish-HAQ-DI (r= 0.276), and DAS28 (r= 0.342) were found, whereas sICAM-1 only correlated with RF (r= 0.445). In RA patients, a significant association with the 721A allele of ICAM1 polymorphism (p= 0.04), was found. In addition, the allele impact (G/A+A/A) of this polymorphism was confirmed, (p= 0.038, OR = 2.3, C.I. 1.1–5.0). sVCAM-1 and sICAM-1 serum levels reflected the clinical status in RA, independently of the ICAM1 and VCAM1 polymorphism. However, the ICAM1 721A allele could be a genetic marker to RA susceptibility.

scholarly journals Periodontal Treatment Improves Serum Levels of Leptin, Adiponectin, and C-Reactive Protein in Thai Patients with Overweight or Obesity

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Notkamon Wanichkittikul ◽  
Penpan Laohapand ◽  
Chayasin Mansa-nguan ◽  
Supanee Thanakun
Keyword(s):  
Disease Status ◽  
Serum Levels ◽  
Normal Weight ◽  
Periodontal Treatment ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Severe Periodontitis ◽  
Study Participants ◽  
Overweight Or Obesity

Periodontitis and overweight or obesity independently change serum levels of leptin, adiponectin, and C-reactive protein (CRP). The aim of this study is to investigate the alterations of serum levels of leptin, adiponectin, and CRP after nonsurgical periodontal treatment (NSPT) in Thai patients with overweight or obesity (Owt/Ob) who did or did not exhibit severe periodontitis (SP) and normal weight (Nwt) patients with or without SP. Two hundred sixty patients were screened; 29 patients were included in this study. The study participants comprised 6 patients with Owt/Ob who exhibited SP, 11 patients with Owt/Ob who did not exhibit SP, 5 Nwt patients with SP, and 7 Nwt patients without SP. Periodontal disease status was evaluated; serum levels of leptin, adiponectin, and CRP were determined by enzyme-linked immunosorbent assay at baseline, as well as at 3 and 6 months after NSPT. At 3 months after NSPT, periodontal status was improved in all groups ( p  < 0.05), except Nwt patients without SP. Serum levels of leptin and CRP were significantly reduced, while serum levels of adiponectin were elevated after NSPT, regardless of bodyweight or waist circumference ( p  < 0.05). Improvement in serum levels of leptin after NSPT was also observed in the Nwt with SP group ( p  = 0.015); these levels did not significantly differ in Nwt patients without SP. NSPT reduces serum levels of leptin and CRP and enhances serum levels of adiponectin in Thai patients with Owt/Ob, irrespective of periodontitis severity. These results suggest a role for periodontal treatment in the systemic inflammatory response of Thai people with Owt/Ob.

scholarly journals Profile of several autoantibodies, complement and vitamin D levels in Brazilian patients with Chikungunya infection

2021 ◽  
Vol 11 (5) ◽  
pp. 979-983
Author(s):  
J. F. Carvalho ◽  
L. R. Cordeiro ◽  
F. F. Silva ◽  
L. Mota ◽  
C. Rodrigues ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Clinical Manifestations ◽  
Serum Levels ◽  
C Reactive Protein ◽  
Complement Components ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Vitamin D Levels

Introduction. Chikungunya virus infection (CKV) may lead to chronic arthritis in up to 40% of the patients. There are previous data regarding positive auto antibodies in CKV. Objective is to systematically evaluate the prevalence of auto antibodies in CKV patients. Methods. All study participants had clinical manifestations being CKV positive at least serologally or by RT-PCR data. The following autoantibodies were assessed: antinuclear antibodies (ANA), anti-dsDNA, anti-Sm, anti-Ro/SS-A, anti-La/SS-B, anti-U1RNP, IgG and IgM anticardiolipin, anticyclic citrullinated peptide (antiCCP), rheumatoid factor (RF), antiribosomal P protein, lupus anticoagulant, anti-Jo-1 and anti-Scl-70. CH100, C3 and C4 complement components, serum levels of immunoglobulins, C-reactive protein, erythrocyte sedimentation rate, alpha1-acid glycoprotein, antithyroglobulin, antithyroperoxidase, TRAb, 25 hydroxyvitamin D (25OHD), dengue and zika IgG and IgM antibodies were also measured. Results. 30 CKV patients were included. Mean age was 59.1±15.7 years, 85% females and 77% Caucasian subjects. Disease duration comprised 4.9±4.0 months. Oligoarthritis was observed in 93% cases. Mean C-reactive protein levels were 10.1±6.8 ng/dL, erythrocyte sedimentation rate — 34.2±19.9 mm/1st hour and alpha1-acid glycoprotein 115.5±52.6 mg/dL. Intramuscular betamethasone depot single dose injection was administered in 54%, prednisone — in 15% and methotrexate — in 23% cases. Importantly, 1/30 (3.3%) cases was positive for ANA, 4/30 (13.3%) — for RF and none was positive for anti-CCP or any other autoantibodies. Complement and immunoglobulin levels were all within the normal range. Low levels of 25OHD were observed in 88% cases.

scholarly journals The Association of Serum IL-10 Levels with the Disease Activity in Systemic-Onset Juvenile Idiopathic Arthritis Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Yu Peng ◽  
Xiaohui Liu ◽  
Zhao Duan ◽  
Junkai Duan ◽  
Yulan Zhou
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Disease Activity ◽  
Sedimentation Rate ◽  
Serum Levels ◽  
Interleukin 10 ◽  
C Reactive Protein ◽  
Clinical Marker ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Systemic Onset

Objectives. Interleukin-10 (IL-10) has been suggested as a biomarker of disease activity in patients with adult-onset Still’s disease (AOSD). In this study, we evaluated the serum IL-10 levels and investigated its clinical relevance in systemic-onset juvenile idiopathic arthritis (SoJIA). Methods. IL-10 levels were determined in 21 patients diagnosed with SoJIA and 35 patients with fever diseases which were suspected as SoJIA, and IL-10 levels were compared between SoJIA patients with regard to disease activity, disease courses, and other biomarkers. Results. Patients with SoJIA had significantly higher levels of IL-10 compared to patients with other febrile diseases. The serum levels of IL-10 were significantly higher in active SoJIA compared to inactive and positively correlated with known disease activity markers such as erythrocyte sedimentation rate (ESR), C-reactive protein level (CRP), ferritin (FER), and IL-6 levels. Moreover, the levels of IL-10 at diagnosis were significantly higher in SoJIA patients with a nonmonocyclic pattern than in patients with a monocyclic pattern. Compared to CRP, ESR, FER, and IL-6, IL-10 levels were superior in predicting monocyclic patients from nonmonocyclic patients. Conclusion. Compared to other febrile diseases, SoJIA patients have markedly higher levels of IL-10 which may assist with diagnosis. And a clear association of serum IL-10 levels with disease activity and disease courses in SoJIA was found. These results suggest that serum IL-10 might be a reliable clinical marker in SoJIA.

scholarly journals Evaluation of the Normal Range of C-reactive Protein and Erythrocyte Sedimentation Rate Serum Level after Total Knee Arthroplasty and Total Hip Arthroplasty in Osteoarthritis Patients without Complications

2021 ◽  
pp. 40-46
Author(s):  
Mohammad Amin Aslani ◽  
Fateme Mirzaee ◽  
Amin Karimi ◽  
Amir Farahanchi Baradaran ◽  
Zohreh Zafarani ◽  
...  
Keyword(s):  
Total Knee Arthroplasty ◽  
Serum Level ◽  
Total Hip Arthroplasty ◽  
Joint Replacement ◽  
Serum Levels ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
The Third ◽  
Erythrocyte Sedimentation ◽  
Total Knee

Aims: Infection is one of the most serious complications of joint replacement surgeries. Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) serum levels are widely used for the evaluation of acute infections after joint replacement surgeries. The aim of our study is to determine normal changes in these inflammatory mediators after total hip arthroplasty (THA) and total knee arthroplasty (TKA). Methodology: In our study, we enrolled 100 patients with mean age of 68 y/o (57–77) and the diagnosis of primary osteoarthritis who underwent joint replacement surgery. ESR and CRP serum levels were measured before surgery, as well as one, three, and five days after surgery and then again 14, 42, and 90 days after surgery. The results were compared in two groups of TKA and THA, and patients who had undergone THA were further divided into cemented and uncemented groups. Results: The CRP serum level increased rapidly and reached its maximum level (130.47) on the third day after surgery and returned to its pre-surgery level at the end of three months in all patients. The ESR serum level reached its peak (142.65) on the fifth day after surgery and then decreased; however, it was still slightly higher than the pre-surgery levels at the end of three months. CRP showed faster and more remarkable level changes following surgery compared to ESR, and this elevation was unrelated to the pre-surgery levels. No significant difference was observed between the cemented and uncemented groups. Conclusion: Changes in CRP and ESR levels after TKA and THA surgeries are reliable indicators for assessment of prosthetic infections. In cases with an elevated CRP serum level on the third day of surgery, a strong suspicion of infection seems completely rational.

Brain-derived neurotrophic factor is associated with coronary macrophage infiltrates in patients with acute coronary syndrome

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
R.A Montone ◽  
M Camilli ◽  
M Russo ◽  
M Del Buono ◽  
F Gurguglione ◽  
...  
Keyword(s):  
Acute Coronary Syndrome ◽  
Neurotrophic Factor ◽  
Serum Levels ◽  
Brain Derived Neurotrophic Factor ◽  
C Reactive Protein ◽  
St Segment Elevation ◽  
Protein Levels ◽  
Reactive Protein ◽  
Coronary Syndrome ◽  
St Segment

Abstract Background Brain-derived neurotrophic factor (BDNF) is a neurotrophine that plays a key role in the regulation of both central and peripheral nervous system. Moreover, BDNF is secreted in multiple tissues and exerts systemic, autocrine, and paracrine effects in the cardiovascular system. Of importance, BDNF expression was enhanced in macrophages and smooth muscle cells in atherosclerotic coronary arteries and may be involved in thrombus formation. Thus, BDNF has been suggested as an important link between inflammation and thrombosis, potentially involved in the pathogenesis of acute coronary syndrome (ACS). Purpose In our study we aimed at assessing serum levels of BDNF in patients with ACS, evaluating differences according to clinical presentation [ST-segment elevation myocardial infarction (STEMI) vs. Non-ST-segment elevation ACS (NSTE-ACS)]. Moreover, we assessed the presence of optical coherence (OCT)-defined macrophage infiltrates (MØI) in the culprit vessel of ACS patients and evaluated their relationship with BDNF levels. Methods ACS patients were prospectively selected. Blood samples were collected at admission and serum levels of BDNF were subsequently assessed. Presence of OCT-defined MØI along the culprit vessel was assessed. Results 166 ACS patients were enrolled [mean age 65.3±11.9 years, 125 (75.3%) male, 109 STEMI, 57 NSTE-ACS]. Serum levels of BDNF were higher among STEMI patients compared with NSTE-ACS [median (IQR) 2.48 pg/mL (1.54–3.34) vs. 2.12 pg/mL (1.34–2.47), p=0.007], while C-reactive protein levels did not differ between the two groups. OCT assessment was performed in 53 patients and MØI were detected in 27 patients. Of importance, patients with MØI in the culprit vessel had higher levels of BDNF compared with patients without MØI [median (IQR) 2.23 pg/mL (1.38–2.53) vs. 1.41 pg/mL (0.93–2.07), p=0.023], while C-reactive protein levels did not differ between the two groups. Of note, at multivariate regression analysis BDNF levels were independent predictor of MØI [OR: 2.20; 95% CI (1.02–4.74), p=0.043]. Conclusions Serum levels of BDNF may reliable identify the presence of local macrophage inflammatory infiltrates in patients with ACS. Moreover, BDNF levels are higher in patients with STEMI compared with NSTE-ACS. Taken together, these data suggest that BDNF may represent an interesting link between local inflammatory activation and enhanced thrombosis in ACS. BDNF serum levels Funding Acknowledgement Type of funding source: None

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