Green synthesis, in vivo and in vitro pharmacological studies of Tamarindus indica based gold nanoparticles

The main aim of the study was to optimization and evaluation of a suitable dosage form i.e., tropical gel containing silver and gold nanoparticles which are synthesized by green synthesis as this is the rapid and better alternative to chemical synthesis. For burn injury systemic route of administration is not suitable due to damaged skin hence tropical route selected to provide controlled effects, low systemic toxicity and easy application. Silver nanoparticles were synthesized by green synthesis by use of neem leaves and gold nanoparticles by Aloe vera. Characterization was done by UV spectroscopy, particle size distribution, TEM and zeta potential. Gel was formulated by simple mixing and stirring method and evaluated for color, homogeneity, Spreadability, viscosity, in vitro release, antimicrobial study and in vivo study. In this study, the combination of Carbopol 934 P and HPMC (ratio 1:3) was found to be ideal formulation (Formulation F5) with optimum viscosity and Spread-ability i.e., 4459 cP and 5.7 gmcm/sec resp. in vitro release was found to be 98.66% in 12 hours. in vitro antimicrobial study and in vivo study on rat showed maximum effects than standard formulations. From this study it can be concluded that, tropical gel is the superior dosage form containing silver and gold nanoparticles which acts as the antimicrobial agents and is effective in treatment of burn surgery.

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Dosimetry and Radioenhancement Comparison of Gold Nanoparticles in Kilovoltage and Megavoltage Radiotherapy using MAGAT Polymer Gel Dosimeter

Journal of Biomedical Physics and Engineering ◽

10.31661/jbpe.v9i2apr.827 ◽

2019 ◽

Vol 9 (2Apr) ◽

Cited By ~ 1

Author(s):

S Farahani ◽

N Riyahi Alam ◽

S Haghgoo ◽

M Khoobi ◽

Gh Geraily ◽

...

Keyword(s):

Gold Nanoparticles ◽

3D Visualization ◽

Energy Levels ◽

Polymer Gel ◽

External Radiotherapy ◽

Dose Enhancement ◽

Internal Radiotherapy ◽

Boost Radiotherapy

Background: Numerous unique characteristics of the nanosized gold, including high atomic number, low toxicity, and high biocompatibility make it one of the most appropriate nanostructures to boost radiotherapy efficacy. Many in-vivo and in-vitro investigations have indicated that gold nanoparticles (AuNPs) can significantly increase tumor injuries in low kilovoltage radiotherapy. While deep-lying tumors require much higher energy levels with greater penetration power, and investigations carried out in megavoltage energy range show contradictory results.Objective: In this study, we quantitatively assess and compare dose enhancement factors (DEFs) obtained through AuNPs under radiation of Cobalt-60 source (1.25MeV) versus Iridium-192 source (0.380 KeV) using MAGAT gel dosimeter.Material and Methods: MAGAT polymer gel in both pure and combined with 0.2 mM AuNPs was synthesized. In order to quantify the effect of energy on DEF, irradiation was carried out by Co-60 external radiotherapy and Ir-192 internal radiotherapy. Finally, readings of irradiated and non-irradiated gels were performed by MR imaging.Result: The radiation-induced R2 (1/T2) changes of the gel tubes doped with AuNPs compared to control samples, upon irradiation of beams released by Ir-192 source showed a significant dose enhancement (15.31% ±0.30) relative to the Co-60 external radiotherapy (5.85% ±0.14).Conclusion: This preliminary study suggests the feasibility of using AuNPs in radiation therapy (RT), especially in low-energy sources of brachytherapy. In addition, MAGAT polymer gel, as a powerful dosimeter, could be used for 3D visualization of radiation dose distribution of AuNPs in radiotherapy.

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Ferula hermonis: A Review of Current Use and Pharmacological Studies of its Sesquiterpene Ester Ferutinin

Current Drug Targets ◽

10.2174/1389450120666191029155053 ◽

2020 ◽

Vol 21 (5) ◽

pp. 499-508 ◽

Cited By ~ 1

Author(s):

Rémi Safi ◽

Marwan El-Sabban ◽

Fadia Najjar

Keyword(s):

Wide Spectrum ◽

Endemic Plant ◽

Anti Cancer ◽

Pharmacological Studies ◽

Sexual Impotence ◽

Novel Applications ◽

Anti Fungal ◽

Sesquiterpene Ester

Ferula hermonis Boiss, is an endemic plant of Lebanon, locally known as “shilsh Elzallouh”. It has been extensively used in the traditional medicine as an aphrodisiac and for the treatment of sexual impotence. Crude extracts and isolated compounds of ferula hermonis contain phytoestrogenic substances having a wide spectrum of in vitro and in vivo pharmacological properties including anti-osteoporosis, anti-inflammatory, anti-microbial and anti-fungal, anti-cancer and as sexual activity enhancer. The aim of this mini-review is to highlight the traditional and novel applications of this plant’s extracts and its major sesquiterpene ester, ferutinin. The phytochemical constituents and the pharmacological uses of ferula hermonis crude extract and ferutinin specifically will be discussed.

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Green Synthesis of Gold Nanoparticles Using Tamarindus indica Extract as a Bioreductant

Innovations in Corrosion and Materials Science (Formerly Recent Patents on Corrosion Science) ◽

10.2174/235209490502151106165432 ◽

2015 ◽

Vol 5 (2) ◽

pp. 93-97 ◽

Cited By ~ 2

Author(s):

Pradyumn Gourav ◽

Syed Hasan

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Tamarindus Indica

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Radiosensitization of Prostate Cancers In Vitro and In Vivo to Erbium-filtered Orthovoltage X-rays Using Actively Targeted Gold Nanoparticles

Scientific Reports ◽

10.1038/s41598-017-18304-y ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 12

Author(s):

Allison M. Khoo ◽

Sang Hyun Cho ◽

Francisco J. Reynoso ◽

Maureen Aliru ◽

Kathryn Aziz ◽

...

Keyword(s):

Gold Nanoparticles ◽

X Rays ◽

Prostate Cancers

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Biocompatibility of gold nanoparticles: In-vitro and In-vivo study

Materials Today Proceedings ◽

10.1016/j.matpr.2020.12.826 ◽

2021 ◽

Vol 42 ◽

pp. 3041-3045

Author(s):

Rua J. Kadhim ◽

Esraa H. Karsh ◽

Zainab J. Taqi ◽

Majid S. Jabir

Keyword(s):

Gold Nanoparticles ◽

In Vivo Study

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In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres

Green Processing and Synthesis ◽

10.1515/gps-2021-0013 ◽

2021 ◽

Vol 10 (1) ◽

pp. 101-111

Author(s):

Rehman Ullah ◽

Sumaira Shah ◽

Zahir Muhammad ◽

Sajjad Ali Shah ◽

Shah Faisal ◽

...

Keyword(s):

Gold Nanoparticles ◽

Muscular Contraction ◽

Surface Plasmon Resonance Peak ◽

Plasmon Resonance Peak ◽

Optical Absorption Peak ◽

Visible Spectra ◽

Scattering Spectra ◽

Dose Dependent

Abstract The current study was designed to investigate the potential of Euphorbia wallichii shoot extract for reducting Au3+ and stabilizing gold nanoparticles. UV-visible spectra of gold nanoparticles showed obvious surface plasmon resonance peak at 548 nm. Microscopy (SEM and TEM) showed spherical dimensions, and the energy dispersive X-ray spectra displayed the strongest optical absorption peak for gold (Au) at 2.1 keV. Dynamic light scattering spectra represent polydispersed mixture with particulate diameter of 2.5–103.2 nm. The IR spectra confirm the potential functional groups of shoot extract responsible for the reduction of Au3+ to gold nanoparticles which exhibit tremendous antibacterial potential of 76.31%, 68.47%, 79.85%, 48.10%, and 65.53% against Escherichia coli, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, respectively. Gold nanoparticles showed markedly elevated fungicidal potency compared to the shoot extract alone against the tested fungal strains. IC50 for 2,2-diphenyl-1-picrylhydrazyl scavenging was 31.52, 18.29, and 15.32 µg/mL at 30, 60, and 90 min of reaction time, respectively. Both shoot extract and nanoparticles revealed 71% mortality at 100 µg/mL, with LD90 values of 310.56 µg/mL. Experimental mice acquired dose-dependent analgesia of 54.21%, 82.60%, and 86.53% when treated with gold nanoparticles at 50, 100, and 200 mg/kg bw. Inhibition of gastrointestinal muscular contraction was 21.16%, 30.49%, and 40.19% in mice feed with 50, 100, and 200 mg/kg bw, respectively.

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The Genus Lagochilus (Lamiaceae): A Review of Its Diversity, Ethnobotany, Phytochemistry, and Pharmacology

Plants ◽

10.3390/plants10010132 ◽

2021 ◽

Vol 10 (1) ◽

pp. 132

Author(s):

Nilufar Z. Mamadalieva ◽

Davlat Kh. Akramov ◽

Ludger A. Wessjohann ◽

Hidayat Hussain ◽

Chunlin Long ◽

...

Keyword(s):

Secondary Metabolites ◽

Chemical Constituents ◽

Iridoid Glycosides ◽

Herbal Medicines ◽

Future Research ◽

South Central ◽

Pharmacological Studies ◽

Central South

The genus Lagochilus (Lamiaceae) is native to Central, South-Central, and Eastern Asia. It comprises 44 species, which have been commonly used as herbal medicines for the treatments of various ailments for thousands of years, especially in Asian countries. This review aims to summarize the chemical constituents and pharmacological activities of species from the genus Lagochilus to unveil opportunities for future research. In addition, we provide some information about their traditional uses, botany, and diversity. More than 150 secondary metabolites have been reported from Lagochilus, including diterpenes, flavonoids, phenolic compounds, triterpenoids, iridoid glycosides, lignans, steroids, alkaloids, polysaccharides, volatile, non-volatile and aromatic compounds, lipids, carbohydrates, minerals, vitamins, and other secondary metabolites. In vitro and in vivo pharmacological studies on the crude extracts, fractions, and isolated compounds from Lagochilus species showed hemostatic, antibacterial, anti-inflammatory, anti-allergic, cytotoxic, enzyme inhibitory, antispasmodic, hypotensive, sedative, psychoactive, and other activities.

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Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy

Pharmaceutics ◽

10.3390/pharmaceutics12030261 ◽

2020 ◽

Vol 12 (3) ◽

pp. 261

Author(s):

Wei Mao ◽

Sol Lee ◽

Ji Un Shin ◽

Hyuk Sang Yoo

Keyword(s):

Gold Nanoparticles ◽

Transfection Efficiency ◽

Cancer Therapeutics ◽

Atom Transfer ◽

Layer By Layer ◽

Dependent Manner ◽

Metallic Particles ◽

Anti Cancer

Surface initiated atom transfer radical polymerization (SI-ATRP) documented a simple but efficient technique to grow a dense polymer layer on any surface. Gold nanoparticles (AuNPs) give a broad surface to immobilize sulfhyryl group-containing initiators for SI-ATRP; in addition, AuNPs are the major nanoparticulate carriers for delivery of anti-cancer therapeutics, since they are biocompatible and bioinert. In this work, AuNPs with a disulfide initiator were polymerized with sulfoethyl methacrylate by SI-ATRP to decorate the particles with anionic corona, and branched polyethyeleneimine (PEI) and siRNA were sequentially layered onto the anionic corona of AuNP by electrostatic interaction. The in vitro anti-cancer effect confirmed that AuNP with anionic corona showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study of tumor-bearing nude mice revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size in comparison to those with free siRNA for 4 weeks. Furthermore, histological examination and gene expression study revealed that the decorated AuNP significantly suppressed c-Myc expression. Thus, we envision that the layer-by-layer assembly on the anionic brushes can be potentially used to incorporate nucleic acids onto metallic particles with high transfection efficiency.

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