Fluorescence Nano Particle Detection in a Liquid Sample Using the Smartphone for Biomedical Application

Abstract In the present work, a Smartphone-based Fluorescence Nanoparticle Detector (SPF-NPD) was developed. This method is intended for use in the identification of biological agents in biomedical applications. Here, an android application-based algorithm was developed to analyze the fluorescent nanoparticle intensity level in a target sample. The setup consists of an LED light source, an Eppendorf tube holder, and a smartphone to acquire the fluorescent intensity level in the sample to enable the detection of pathogens within few seconds. High-resolution cameras available on recent smartphones have made live detection more accurate and convenient for healthcare applications. The concept of fluorescent nanoparticle detection with a smartphone has led to a portable device and having potential application in healthcare. In this proposed method the intensity level is analyzed with 5 pixels algorithm, the center pixel followed by four immediate neighbours’ pixels which can analyze with minimal sample quantity. Also, the robustness of the developed algorithm was verified with various megapixel camera ranges from 8 MP to 20 MP.

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On-chip Microscopy and Nano-particle Detection Using Ultraviolet Light

Conference on Lasers and Electro-Optics ◽

10.1364/cleo_at.2017.ath1a.5 ◽

2017 ◽

Author(s):

Mustafa Ugur Daloglu ◽

Aniruddha Ray ◽

Zoltan Gorocs ◽

Matthew Xiong ◽

Ravinder Malik ◽

...

Keyword(s):

Ultraviolet Light ◽

Nano Particle ◽

Particle Detection ◽

On Chip

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SYNTHESIS AND CHARACTERIZATION OF CALCITE NANO-PARTICLE DERIVED FROM COCKLE SHELL FOR CLINICAL APPLICATION

ASEAN Engineering Journal ◽

10.11113/aej.v10.15538 ◽

2020 ◽

Vol 10 (1) ◽

pp. 49-54

Author(s):

Nguyen Xuan Thanh Tram

Keyword(s):

Clinical Application ◽

Biomedical Application ◽

Raw Materials ◽

Tooth Surface ◽

Synthesis And Characterization ◽

Nano Particle ◽

X Ray Diffraction ◽

Transmission Electron ◽

Size And Morphology

Calcite nano-particle is suggested as an extremely effective material in the biomedical due to their shape, non-toxic properties and being easily metabolized by cells. In clinical application, calcite nano- particle has to be useful for recovering the surface of tooth. The surface of tooth was often damaged due to wrong tooth care treatment, dental caries and periodontal disease. Because of tooth surface destruction, it could lead to dental hypersensitivity. It is desirable to find a suitable source for supplying calcium to fill in dentinal tubules and later regenerate the tooth surface. This study demonstrated the synthesis and characterization of calcite nano-particle for its application in dental hypersensitivity treatment. Cockleshell was found to be a suitable source to synthesize calcite. Using cockleshell as raw materials to produce calcite nanoparticle is meaningful for both environmental protection and biomedical application. Therefore, in this study, calcite nano-particle was synthesized using cockleshell as a reproducibility source of calcium carbonate (CaCO3) and chitosan as a size-reducing agent. X-ray Diffraction (XRD) was used to characterize phase composition of raw materials, as-prepared powder and calcined powder. Transmission Electron Microscopy (TEM) was used to evaluate the size and morphology of powder. Results indicated that calcite nano-particle with size about 50-100 nm could be obtained. It would be expected to consider for further studies in clinical application.

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Real time micro/nano particle detection and tracking with nanosecond resolution

10.1117/12.826587 ◽

2009 ◽

Author(s):

Feng Qian ◽

Qi Song ◽

En-kuang Tien ◽

Ozdal Boyraz

Keyword(s):

Real Time ◽

Nano Particle ◽

Particle Detection ◽

Detection And Tracking

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SEM evaluation of the TEM cold-stage double-film specimen

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111471 ◽

1984 ◽

Vol 42 ◽

pp. 272-273

Author(s):

Jayesh Bellare

Keyword(s):

Spatial Distribution ◽

Sample Preparation ◽

Sample Thickness ◽

Specimen Preparation ◽

Preparation Technique ◽

Liquid Sample ◽

Thin Specimen ◽

Sample Preparation Technique ◽

Cold Stage ◽

Film Specimen

Seeing is believing, but only after the sample preparation technique has received a systematic study and a full record is made of the treatment the sample gets.For microstructured liquids and suspensions, fast-freeze thermal fixation and cold-stage microscopy is perhaps the least artifact-laden technique. In the double-film specimen preparation technique, a layer of liquid sample is trapped between 100- and 400-mesh polymer (polyimide, PI) coated grids. Blotting against filter paper drains excess liquid and provides a thin specimen, which is fast-frozen by plunging into liquid nitrogen. This frozen sandwich (Fig. 1) is mounted in a cooling holder and viewed in TEM.Though extremely promising for visualization of liquid microstructures, this double-film technique suffers from a) ireproducibility and nonuniformity of sample thickness, b) low yield of imageable grid squares and c) nonuniform spatial distribution of particulates, which results in fewer being imaged.

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