Sensitivity of mitochondria isolated from liver and kidney of rat and bovine to lipid peroxidation: A comparative study of light emission and fatty acid profiles

2005 ◽  
Vol 280 (1-2) ◽  
pp. 77-82 ◽  
Author(s):  
Mariana Gavazza ◽  
Mónica Marmunti ◽  
Angel Catalá
2009 ◽  
Vol 25 (2) ◽  
pp. 308-316 ◽  
Author(s):  
I. Oborna ◽  
G. Wojewodka ◽  
J.B. De Sanctis ◽  
H. Fingerova ◽  
M. Svobodova ◽  
...  

2014 ◽  
Vol 91 (8) ◽  
pp. 1471-1476 ◽  
Author(s):  
Caixia Wang ◽  
Wenshui Xia ◽  
Yanshun Xu ◽  
Qixing Jiang ◽  
Peipei Yu

1997 ◽  
Vol 78 (4) ◽  
pp. 655-669 ◽  
Author(s):  
Kazuhiro Kubo ◽  
Morio Saito ◽  
Tadahiro Tadokoro ◽  
Akio Maekawa

To examine the effects of dietary docosahexaenoic acid (DHA) on the potential changes in endogenous lipid peroxidation in the liver and kidney, diets containing a fixed amount of vitamin E (VE; RRR-α-tocopherol equivalent; 134 mg/kg diet) and a graded amount of DHA at the levels of 0, 1.0, 3.4 and 8.7% of total dietary energy were fed to rats for 14 d (Expt 1). In Expt 2, diets containing a fixed amount of DHA (8.7% of total dietary energy) and a graded amount of VE at the levels of 54, 134 and 402 mg/kg were fed to rats for 15 d. In Expt 1 it was found that endogenous lipid peroxide contents of the liver and kidney, as measured by thiobarbituric acid value and chemiluminescence intensity, were higher, and their α-tocopherol contents lower than those of the controls, with a gradual increase and decrease in values respectively as the dietary DHA level increased (Expt 1). However, the contents of water-soluble antioxidants, i.e. ascorbic acid and non-protein-SH (glutathione), increased with increases in the dietary DHA level, while the Se-dependent glutathione peroxidase (EC 1.11.1.9) activities did not change or tended to be lower. When the graded level of VE was given to rats in Expt 2, lipid peroxide contents in the liver and kidney did not change significantly in response to the increasing levels of dietary VE, although their α-tocopherol contents were higher than control values, increasing with increases in the dietary VE levels. The lipid peroxide scavengers other than a-tocopherol changed similarly to those in Expt 1. The results obtained in Expts 1 and 2 indicate that DHA enhances the susceptibility of the liver and kidney to lipid peroxidation concomitant with higher levels of DHA in these tissues, as shown by the fatty acid composition. In addition, VE is unable to protect membranes of the liver and kidney rich in DHA from lipid peroxidation, even after ingestion of the highest level of VE. However, the liver lipid peroxide content of the group given the highest level of DHA was not as high as expected, based on the peroxidizability index which was calculated from the fatty acid composition of the liver lipid.


2001 ◽  
Vol 30 (9) ◽  
pp. 1029-1035 ◽  
Author(s):  
Joseph M Burczynski ◽  
Scott J Southard ◽  
Jill R Hayes ◽  
Penelope A Longhurst ◽  
Howard D Colby

2012 ◽  
Vol 114 (9) ◽  
pp. 1036-1043 ◽  
Author(s):  
Hanne Devle ◽  
Ingjerd Vetti ◽  
Carl Fredrik Naess-Andresen ◽  
Elling-Olav Rukke ◽  
Gerd Vegarud ◽  
...  

2010 ◽  
Vol 21 (2) ◽  
pp. 210-223 ◽  
Author(s):  
SERAP GÖNCÜ KARAKÖK ◽  
YESIM OZOGUL ◽  
MÜCELLA SALER ◽  
FATIH OZOGUL

2014 ◽  
Vol 65 (4) ◽  
pp. e048 ◽  
Author(s):  
E. Vargas-Bello-Pérez ◽  
C. Aguilar ◽  
P. Toro-Mujica ◽  
R. Vera ◽  
C. Ugalde ◽  
...  

2012 ◽  
Vol 23 (1) ◽  
pp. 8
Author(s):  
M. Marmunti ◽  
A. M. Gutiérrez ◽  
M. Gavazza ◽  
S. Williams ◽  
A. Palacios

<p>Objectives of this study were to determine the fatty acid composition and to analyze the sensitivity to lipid peroxidation of different boar fresh semen samples from two herds, H1 and H2. Lipid peroxidation was evaluated using chemilu­minescence (cpm/mg of protein) and fatty acid profile by means of gas chromatography. The saturated fatty acid content found in the analyzed spermatozoa was approximately 43% in H1 and 33% in H2, whereas the total unsaturated fatty acid content was 47% in H1 and 59% in H2. When control and ascorbate-Fe++ dependent samples were compared, it was observed a significant increase in light emission. Consequently, significant decrease in the percentage of the polyunsaturated fatty acids was determined, being more affected: C22: 5 n6 and C22: 6 n3 in both herds, whereas C20: 4 n6 and C22: 4 n6 only in H2. The great amounts of polyun­saturated fatty acids found in H2 samples could be related to the loss of acrosomal integrity. Our results indicate that boar semen contains great amounts of polyunsaturated fatty acid in its composition, which were vulnerable to the lipid peroxidation.</p>


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