Effects In Vitro of Guanidinoacetate on Adenine Nucleotide Hydrolysis and Acetylcholinesterase Activity in Tissues from Adult Rats

2008 ◽  
Vol 33 (6) ◽  
pp. 1129-1137 ◽  
Author(s):  
Roselia Maria Spanevello ◽  
Angela Terezinha de Souza Wyse ◽  
Cinthia Melazzo Mazzanti ◽  
Roberta Schmatz ◽  
Naiara Stefanello ◽  
...  
Keyword(s):  
Adenine Nucleotide ◽  
Acetylcholinesterase Activity ◽  
Adult Rats ◽  
Nucleotide Hydrolysis

The effect of ebselen on adenine nucleotide hydrolysis by platelets from adult rats

2004 ◽  
Vol 148 (1-2) ◽  
pp. 93-99 ◽  
Author(s):  
Cristina Ribas Fürstenau ◽  
Ana Paula Spier ◽  
Bárbara Rücker ◽  
Simone Luisa Berti ◽  
Ana Maria Oliveira Battastini ◽  
...  
Keyword(s):  
Adenine Nucleotide ◽  
Adult Rats ◽  
Nucleotide Hydrolysis

Hepatotrophic effects of insulin on glucose, glycogen, and adenine nucleotides in hepatocytes isolated from fed adult rats

1980 ◽  
Vol 58 (10) ◽  
pp. 1004-1011 ◽  
Author(s):  
Khursheed N. Jeejeebhoy ◽  
Joseph Ho ◽  
Rajni Mehra ◽  
Alan Bruce-Robertson
Keyword(s):  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Energy Charge ◽  
Close Correlation ◽  
Adult Rats ◽  
Fed State ◽  
Adenine Nucleotide Pool ◽  
Intracellular Glucose

In vivo observations have suggested that there is an hepatotrophic effect of insulin. By contrast, subsequent in vitro work, using the isolated perfused liver system, showed no effect or indeterminate effects of insulin on the transport of glucose into the hepatocyte. However because this system may not have endured long enough to show such an influence we explored the transport of glucose using a 48-h suspension culture of hepatocytes isolated from young adult fed rats, the suspension being infused continuously with insulin at a rate approximating the maximum entering portal blood in the fed state. (In a separate study phloridzin was added after 2 h of incubation.) DNA, intracellular glucose and its inward transport, glycogen, and the adenine nucleotides were measured at intervals. By comparison with control or untreated cells, insulin-treated cells showed significantly more DNA and intracellular glucose, and the differences were abolished by phloridzin. Glucose transport rates fell to low values in untreated controls and still lower with insulin plus phloridzin. but the initial rate was maintained to the end (48 h) by insulin alone. Results for glycogen were similar to those for intracellular glucose. There was a close correlation (r = 0.96) between these two. The total adenine nucleotide pool and the concentration of ATP were maintained for about 24 h and fell to half their initial values by 48 h. Insulin had increased these concentrations significantly by 6 h. Although concentrations of ADP and AMP decreased gradually in all groups of cells, insulin enhanced the level of ADP by 12 h but had no measurable effect on that of AMP. The energy charge increased slightly throughout incubation but more so (by 6 h) in the presence of insulin. In conclusion the data support the concept that in the longer term (> 12 h) insulin in the portal circulation maintains the characteristic free permeability of the hepatocyte to glucose and this permits a variety of effects related to glucose entry into the hepatocyte.

scholarly journals Caffeic acid treatment alters the extracellular adenine nucleotide hydrolysis in platelets and lymphocytes of adult rats

2013 ◽  
Vol 56 ◽  
pp. 459-466 ◽  
Author(s):  
Javed Anwar ◽  
Roselia Maria Spanevello ◽  
Victor Camera Pimentel ◽  
Jessié Gutierres ◽  
Gustavo Thomé ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Acid Treatment ◽  
Adenine Nucleotide ◽  
Adult Rats ◽  
Nucleotide Hydrolysis

In vitro Effect of Central Nervous System Active Drugs on the ATPase-ADPase Activity and Acetylcholinesterase Activity from Cerebral Cortex of Adult Rats

1998 ◽  
Vol 31 (4) ◽  
pp. 563-567 ◽  
Author(s):  
Claudia Kuplich Barcellos ◽  
Maria Rosa Chitolina Schetinger ◽  
Renato Dutra Dias ◽  
João José Freitas Sarkis
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cerebral Cortex ◽  
Acetylcholinesterase Activity ◽  
Adult Rats ◽  
Vitro Effect

Previous treatment with ebselen and vitamin E alters adenine nucleotide hydrolysis in platelets from adult rats experimentally demyelinated with ethidium bromide

Life Sciences ◽  
2007 ◽  
Vol 81 (3) ◽  
pp. 241-248 ◽  
Author(s):  
Cinthia M. Mazzanti ◽  
Roselia M. Spanevello ◽  
André Morsch ◽  
Rafael Zanin ◽  
Vanessa Battisti ◽  
...  
Keyword(s):  
Vitamin E ◽  
Ethidium Bromide ◽  
Adenine Nucleotide ◽  
Previous Treatment ◽  
Adult Rats ◽  
Nucleotide Hydrolysis

In vitro effect of homocysteine on nucleotide hydrolysis by blood serum from adult rats

2006 ◽  
Vol 160 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Ana Elisa Böhmer ◽  
Daniela Pochmann ◽  
João J.F Sarkis
Keyword(s):  
Blood Serum ◽  
Adult Rats ◽  
Nucleotide Hydrolysis ◽  
Vitro Effect

Effect of Thiazolidin-4-one Against Lipopolysaccharide—Induced Oxidative Damage, and Alterations in Adenine Nucleotide Hydrolysis and Acetylcholinesterase Activity in Cultured Astrocytes

2022 ◽  
Author(s):  
Fernando Lopez Alvez ◽  
Natália Pontes Bona ◽  
Nathalia Stark Pedra ◽  
Daniel Schuch da Silva ◽  
Wilson João Cunico ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Adenine Nucleotide ◽  
Acetylcholinesterase Activity ◽  
Nucleotide Hydrolysis ◽  
Cultured Astrocytes

Acetylcholinesterase Inhibitory Potential and Antioxidant Activities of Five Cultivars of Rosa Damascena Mill. From Isparta, Turkey

2020 ◽  
Vol 18 (4) ◽  
pp. 354-359
Author(s):  
Shirin Tarbiat ◽  
Azize Simay Türütoğlu ◽  
Merve Ekingen
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Free Radical ◽  
Neurodegenerative Disorder ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acetylcholinesterase Activity ◽  
Rosa Damascena ◽  
Free Radical Damage

Alzheimer's disease is a neurodegenerative disorder characterized by memory loss and impairment of language. Alzheimer's disease is strongly associated with oxidative stress and impairment in the cholinergic pathway, which results in decreased levels of acetylcholine in certain areas of the brain. Hence, inhibition of acetylcholinesterase activity has been recognized as an acceptable treatment against Alzheimer's disease. Nature provides an array of bioactive compounds, which may protect against free radical damage and inhibit acetylcholinesterase activity. This study compares the in vitro antioxidant and anticholinesterase activities of hydroalcoholic extracts of five cultivars of Rosa Damascena Mill. petals (R. damascena 'Bulgarica', R. damascena 'Faik', R. damascena 'Iranica', R. damascena 'Complex-635' and R. damascena 'Complex-637') from Isparta, Turkey. The antioxidant activities of the hydroalcoholic extracts were tested for ferric ion reduction and DPPH radical scavenging activities. The anti-acetylcholinesterase activity was also evaluated. All rose cultivars showed a high potency for scavenging free radical and inhibiting acetylcholinesterase activity. There was a significant correlation between antioxidant and acetylcholinesterase inhibitory activity. Among cultivars, Complex-635 showed the highest inhibitory effect with an IC50 value of 3.92 µg/mL. Our results suggest that all these extracts may have the potential to treat Alzheimer's disease with Complex-635 showing more promise.

Spirocyclohexadienones as an Uncommon Scaffold for Acetylcholinesterase Inhibitory Activity

2019 ◽  
Vol 15 (4) ◽  
pp. 373-382 ◽  
Author(s):  
Ralph C. Gomes ◽  
Renata P. Sakata ◽  
Wanda P. Almeida ◽  
Fernando Coelho
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Population Aging ◽  
Acetylcholinesterase Inhibitors ◽  
Acetylcholinesterase Activity ◽  
Docking Study ◽  
Biological Properties ◽  
Ache Inhibitor ◽  
Molecular Docking Study

Background: The most important cause of dementia affecting elderly people is the Alzheimer’s disease (AD). Patients affected by this progressive and neurodegenerative disease have severe memory and cognitive function impairments. Some medicines used for treating this disease in the early stages are based on inhibition of acetylcholinesterase. Population aging should contribute to increase the cases of patients suffering from Alzheimer's disease, thus requiring the development of new therapeutic entities for the treatment of this disease. Methods: The objective of this work is to identify new substances that have spatial structural similarity with donepezil, an efficient commercial drug used for the treatment of Alzheimer's disease, and to evaluate the capacity of inhibition of these new substances against the enzyme acetylcholinesterase. Results: Based on a previous results of our group, we prepared a set of 11 spirocyclohexadienones with different substitutions patterns in three steps and overall yield of up to 59%. These compounds were evaluated in vitro against acetylcholinesterase. We found that eight of them are able to inhibit the acetylcholinesterase activity, with IC50 values ranging from 0.12 to 12.67 µM. Molecular docking study indicated that the spirocyclohexadienone, 9e (IC50 = 0.12 µM), a mixedtype AChE inhibitor, showed a good interaction at active site of the enzyme, including the cationic (CAS) and the peripheral site (PAS). Conclusion: We described the first study aimed at investigating the biological properties of spirocyclohexadienones as acetylcholinesterase inhibitors. Thus, we have identified an inhibitor, which provided valuable insights for further studies aimed at the discovery of more potent acetylcholinesterase inhibitors.

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