Composition and Biological Activity of Furanoquinolizidine Alkaloids from Yellow Water-Lily (Nuphar Lutea L. Smith) Rhizomes

2019 ◽  
Vol 53 (6) ◽  
pp. 553-558
Author(s):  
V. I. Sheichenko ◽  
O. N. Tolkachev ◽  
V. I. Osipov ◽  
O. P. Sheichenko ◽  
V. V. Anufrieva ◽  
...  
Keyword(s):  
Biological Activity ◽  
Water Lily ◽  
Nuphar Lutea

scholarly journals Nuphar lutea Extracts Exhibit Anti-Viral Activity against the Measles Virus

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1657
Author(s):  
Hila Winer ◽  
Janet Ozer ◽  
Yonat Shemer ◽  
Irit Reichenstein ◽  
Brit Eilam-Frenkel ◽  
...  
Keyword(s):  
Measles Virus ◽  
Acute Infection ◽  
Vero Cells ◽  
Transcriptional Level ◽  
Virus Infectivity ◽  
Water Lily ◽  
African Green Monkey Kidney ◽  
Viral Rnas ◽  
Nuphar Lutea ◽  
Infected Cells

Different parts of Nuphar lutea L. (yellow water lily) have been used to treat several inflammatory and pathogen-related diseases. It has shown that Nuphar lutea extracts (NUP) are active against various pathogens including bacteria, fungi, and leishmanial parasites. In an effort to detect novel therapeutic agents against negative-stranded RNA (- RNA) viruses, we have tested the effect of a partially-purified alkaloid mixture of Nuphar lutea leaves on the measles virus (MV). The MV vaccine’s Edmonston strain was used to acutely or persistently infect cells. The levels of several MV proteins were detected by a Western blot and immunocytochemistry. Viral RNAs were quantitated by qRT-PCR. Virus infectivity was monitored by infecting African green monkey kidney VERO cells’ monolayers. We showed that NUP protected cells from acute infection. Decreases in the MV P-, N-, and V-proteins were observed in persistently infected cells and the amount of infective virus released was reduced as compared to untreated cells. By examining viral RNAs, we suggest that NUP acts at the post-transcriptional level. We conclude, as a proof of concept, that NUP has anti-viral therapeutic activity against the MV. Future studies will determine the mechanism of action and the effect of NUP on other related viruses.

scholarly journals 6,6′-Dihydroxythiobinupharidine (DTBN) Purified from Nuphar lutea Leaves Is an Inhibitor of Protein Kinase C Catalytic Activity

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2785
Author(s):  
Kamran Waidha ◽  
Nikhil Ponnoor Anto ◽  
Divya Ram Jayaram ◽  
Avi Golan-Goldhirsh ◽  
Saravanakumar Rajendran ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Kinase Activity ◽  
Kinase Domain ◽  
Water Lily ◽  
Activity Data ◽  
Nuphar Lutea ◽  
Kinase C ◽  
Disease Biology

Water lily (Nuphar) bioactive extracts have been widely used in traditional medicine owing to their multiple applications against human ailments. Phyto-active Nuphar extracts and their purified and synthetic derivatives have attracted the attention of ethnobotanists and biochemists. Here, we report that 6,6′-dihydroxythiobinupharidine (DTBN), purified from extracts of Nuphar lutea (L.) Sm. leaves, is an effective inhibitor of the kinase activity of members of the protein kinase C (PKC) family using in vitro and in silico approaches. We demonstrate that members of the conventional subfamily of PKCs, PKCα and PKCγ, were more sensitive to DTBN inhibition as compared to novel or atypical PKCs. Molecular docking analysis demonstrated the interaction of DTBN, with the kinase domain of PKCs depicting the best affinity towards conventional PKCs, in accordance with our in vitro kinase activity data. The current study reveals novel targets for DTBN activity, functioning as an inhibitor for PKCs kinase activity. Thus, this and other data indicate that DTBN modulates key cellular signal transduction pathways relevant to disease biology, including cancer.

scholarly journals Reproductive potential of yellow water-lily (Nuphar lutea) in the conditions of lake ecosystems

2020 ◽  
Vol 28 (1) ◽  
pp. 60-67
Author(s):  
O. A. Lebedeva ◽  
E. A. Belyakov ◽  
A. G. Lapirov
Keyword(s):  
Reproductive Potential ◽  
Water Bodies ◽  
Morphological Parameters ◽  
Seed Productivity ◽  
Experimental Conditions ◽  
Water Lily ◽  
Lake Ecosystems ◽  
Nuphar Lutea ◽  
Highly Active ◽  
Morphologic Characteristics

Nuphar lutea (L.) Sm. (Nymphaeaceae Salisb.) belongs to the category of highly active cenose-forming plants in water bodies and water flows. The material for study of morphological peculiarities and seed productivity of the fruits of this species was collected mainly in the lake ecosystems of the Central Part of European Russia and Republic of Belarus. In the study we used biomorphological and ontogenetic approaches. Seed productivity was surveyed by direct count of number of seeds in the fruit. By abundance (in descending order), the significant reliable inter-lake differences in a number of morphological parameters of the fruit fall into the following sequence: number of rays of stigma of fruit (in six pairs of lakes) > length of fruit (in four pairs of lakes) > diameter of the stigma of fruit (in three pairs of lakes) > diameter of fruit (in two pairs of lakes) > length of the neck of the fruit (in one pair of lakes). Intra-regional differences in certain morphological parameters of fruits (by number of rays of stigmas) were most notably manifested only in the Belarus lakes which are similar by trophic status. All the differences in the fruits’ morphology could be due to differences in the habitat by the amount of nutrients in water and soil. The amount of seed productivity of the fruits from N. lutea varies broadly. Analysis of this parameter depending on the character of soils in which the plants grew indicated reliable results only in the case of muddy (296 ± 81) and sandy soils (179 ± 13). We determined that distribution of generative diasporas of N. lutea across large distances is related not only to presence of floating ability in the seeds, but also the “multi-step” process of their release: first – mericarps from fruits, and then seeds from mericarps. In the experimental conditions, most seeds directly sunk to the bed (70%) and the rest continued to float on the surface of the water during a month. Low germination of the seeds of the water-lily in the laboratory conditions (4–6%) with use of different means and terms of dry stratification was due to the fact that they have an organic morphophysiological intermediate type of rest. Having such a mechanism causes portioned and prolonged germination, allowing the species to survive in non-favourable conditions and accumulate generative diaspores in the soil. Despite the fact that the initial stages of ontogenesis in the laboratory and natural conditions have no significant differences, we found polyvariance in their tempo of development. First of all, this is related to different dates of transition of the plant from one age state to another. In the case of N. lutea, one should also note the morphological polyvariance of ontogenesis related to change in morphologic characteristics of plants depending on the water level. The obtained results give a more complete picture of the peculiarities of generative reproduction of N. lutea, necessary for understanding the ecosystem role of this plant and its impact on biological processes in water bodies.

scholarly journals Resources of Nuphar lutea (L.) Sibth. & Sm. in mid-eastern Poland as a potential source of herbal raw material

2020 ◽  
Vol 75 (3) ◽  
pp. 103-116
Author(s):  
PIOTR SUGIER ◽  
BOGDAN LORENS
Keyword(s):  
Secondary Metabolites ◽  
Raw Material ◽  
Rapid Rate ◽  
Small Lakes ◽  
Water Lily ◽  
Nuphar Lutea ◽  
Potential Source ◽  
Field Investigations ◽  
Considerable Concentration ◽  
And Function

The aim of this study was to characterize the phytocoenoses with a share of Nuphar lutea (L.) Sibth. & Sm. and to evaluate the area of patches of Nupharo-Nymphaeetum albae Tomaszewicz 1977 as well as resources of the yellow water lily that can be used as a source of valuable medicinal raw material. The field investigations were carried out in the summer seasons of 2009–2018, and fifty-eight lakes were included in this study. Nuphar lutea is a very interesting plant species due to considerable concentration of secondary metabolites and their biological activity. Therefore, under conditions of eastern Poland, it can be a valuable raw material for pharmaceutical purposes. The largest Nupharo-Nymphaeetum albae phytocoenoses with N. lutea as the main floristic component are localized especially in retention reservoirs connected with the Wieprz-Krzna Canal and other lakes of a natural character. They are often very shallow basins. Therefore, removal of a biomass, especially in the case of small lakes that overgrow at a very rapid rate, can significantly slow down their shallowing and they will remain and function in the landscape over a longer time. Harvesting the N. lutea leaves can decelerate the process of shallowing of water bodies, especially in the case of retention reservoirs, and may contribute to their better functioning. Collected raw material can be a source of valuable secondary metabolites suitable for the pharmaceutical industry.

scholarly journals TO THE METHOD OF DETERMINING THE LEAF AREA OF PLANTS OF THE FAMILY NYMPHAEACEAE SALISB.

2021 ◽  
pp. 138-147
Author(s):  
A. Chernova
Keyword(s):  
Leaf Area ◽  
Leaf Blade ◽  
Cost Effective ◽  
Point Of View ◽  
Water Lily ◽  
Volga River ◽  
Important Indicator ◽  
Nuphar Lutea ◽  
Received Power ◽  
Specially Protected Natural Areas

Leaf area is an important indicator that is closely related to the size of the assimilating surface, photosynthesis, respiration, transpiration, specific leaf area, and production. The purpose of this work is to obtain regression models for determining the leaf area of widespread hydrophytes – Nuphar lutea (L.) Smith and Nymphaea candida C. Presl. These plants have a high ecological valence and make a significant contribution to the overall productivity of reservoirs. Collection of floating leaves of water lilies was carried out in 2019 in the gulf of the Volga river in the Tver region (56°58'50.4", 37°27'45.2"). A total of 108 leaves of Nuphar lutea and 170 – Nymphaea candida were collected. Main parameters: the length of the leaf blade from the attachment point of the petiole to the tip (l1), the total length (l2) and width (w) of the leaf blade were measured with an accuracy of 0.1 cm. The actual leaf area was determined by a Planix 7 planimeter. Correlation and regression analyses were used to analyze the data. The growth of water lily leaves is uniform. Regression analysis revealed the dependence of the actual leaf area (LA) on morphometric indicators l1, l2, and w. For Nuphar lutea: LAN.l=2.12∙l11.81; LAN.l=0.64∙l21.95; LAN.l=0.93∙w2.05. For Nymphaea candida: LAN.c=3.88∙l11.79; LAN.c=0.85∙l21.94; LAN.c=0.93∙w1.96 The received power equation is fair from a biological point of view and correct with mathematical. They can be used in the field without causing damage to plant communities. This is a fast, reliable and cost-effective method. It allows you to monitor, assess the degree of overgrowth of reservoirs, predict the further development of communities, and allows you to conduct research on specially protected natural areas.

Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

1973 ◽  
Vol 31 ◽  
pp. 378-379
Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Human Breast Cancer ◽  
Osteogenic Sarcoma ◽  
Human Tumor ◽  
Structural Similarity ◽  
Cell Tumor ◽  
Human Breast ◽  
Human Tumor Cell ◽  
Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

Sign in / Sign up

Export Citation Format

Share Document