Proteomic Analysis of Heat Stress Response in Leaves of Radish (Raphanus sativus L.)

Abstract Background: As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Result: In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper seedlings in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. Conclusion: To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. This study provided new insights into the molecular mechanisms involved in heat tolerance of pepper and might offer supportive reference for the breeding of new pepper variety with heat resistance.

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Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot

Planta ◽

10.1007/s00425-018-2846-5 ◽

2018 ◽

Vol 247 (5) ◽

pp. 1109-1122 ◽

Cited By ~ 6

Author(s):

Ronghua Wang ◽

Yi Mei ◽

Liang Xu ◽

Xianwen Zhu ◽

Yan Wang ◽

...

Keyword(s):

Heat Stress ◽

Proteomic Analysis ◽

Regulatory Network ◽

Raphanus Sativus ◽

Raphanus Sativus L

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iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings

PeerJ ◽

10.7717/peerj.11509 ◽

2021 ◽

Vol 9 ◽

pp. e11509

Author(s):

Jing Wang ◽

Chengliang Liang ◽

Sha Yang ◽

Jingshuang Song ◽

Xuefeng Li ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Proteomic Analysis ◽

Negative Impact ◽

Bioinformatic Analysis ◽

Growth And Yield ◽

Vegetable Crops ◽

Ros Scavenging ◽

Heat Stress Response ◽

Quantitative Proteomic Analysis

Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180.

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1083 Proteomic analysis reveals increased Nrf2-mediated oxidative stress response in adipose tissue of late pregnant dairy cows during summer heat stress

Journal of Animal Science ◽

10.2527/jam2016-1083 ◽

2016 ◽

Vol 94 (suppl_5) ◽

pp. 519-520

Author(s):

M. Zachut ◽

G. Kra ◽

G. Friedlander ◽

Y. Levin

Keyword(s):

Oxidative Stress ◽

Adipose Tissue ◽

Heat Stress ◽

Stress Response ◽

Dairy Cows ◽

Proteomic Analysis ◽

Oxidative Stress Response ◽

Summer Heat

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Single Gene Consistently Associated with Heat Stress Response in Three Distinct Chicken Lines

10.31274/ans_air-180814-293 ◽

2017 ◽

Author(s):

Xi Lan ◽

John C. F. Hsieh ◽

Carl J. Schmidt ◽

Qing Zhu ◽

Susan J. Lamont

Keyword(s):

Heat Stress ◽

Stress Response ◽

Single Gene ◽

Heat Stress Response

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Faculty Opinions recommendation of A membrane-tethered transcription factor defines a branch of the heat stress response in Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.2542956.2191054 ◽

2010 ◽

Author(s):

David G Oppenheimer

Keyword(s):

Arabidopsis Thaliana ◽

Transcription Factor ◽

Heat Stress ◽

Stress Response ◽

Heat Stress Response

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The Heat Stress Response and Diabetes: More Room for Mitochondrial Implication

Current Pharmaceutical Design ◽

10.2174/1381612822666160203114738 ◽

2016 ◽

Vol 22 (18) ◽

pp. 2619-2639 ◽

Cited By ~ 3

Author(s):

Biljana Miova ◽

Maja Dimitrovska ◽

Suzana Dinevska-Kjovkarovska ◽

Juan V. Esplugues ◽

Nadezda Apostolova

Keyword(s):

Heat Stress ◽

Stress Response ◽

Heat Stress Response

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Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)

Scientific Reports ◽

10.1038/s41598-021-95899-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Bin Huang ◽

Zhinuo Huang ◽

Ruifang Ma ◽

Jialu Chen ◽

Zhijun Zhang ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Heat Shock ◽

Gene Family ◽

Regulatory Elements ◽

Moso Bamboo ◽

Naphthalene Acetic Acid ◽

Plant Responses ◽

Heat Stress Response ◽

Regulatory Pathways

AbstractHeat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of the HSF gene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bamboo HSF genes could be divided into three major subfamilies; HSFs from the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. All HSF genes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associated cis-regulatory elements were identified in the HSF upstream promoter sequences. Synteny analysis indicated that the HSFs in the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bamboo HSF gene family. Transcriptome data indicated that the expression of a number of PeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number of HSF genes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bamboo HSF gene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.

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