Flaxseed Effects on Inflammation Regulatory Gene Expressions in an Obese Animal Model

2021 ◽  
Author(s):  
Michael Mann ◽  
Yeong Rhee
Keyword(s):  
Animal Model ◽  
Regulatory Gene ◽  
Gene Expressions ◽  
Obese Animal

Effect of Sleeve Gastrectomy on Angiogenesis and Adipose Tissue Health in an Obese Animal Model of Type 2 Diabetes

2019 ◽  
Vol 29 (9) ◽  
pp. 2942-2951 ◽  
Author(s):  
Hans Eickhoff ◽  
Tiago Rodrigues ◽  
Inês Neves ◽  
Daniela Marques ◽  
Diana Ribeiro ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Animal Model ◽  
Adipose Tissue ◽  
Sleeve Gastrectomy ◽  
Obese Animal

Erythropoietin Lacking Hypoxia Responsive Element in 3′ Untranslated Region Stimulates Hepatic Erythropoiesis in Anemic Xenopus.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4057-4057
Author(s):  
Youichi Aizawa ◽  
Hironori Nishikawa ◽  
Takehito Okui ◽  
Nami Nogawa-Kosaka ◽  
Nobuyoshi Kosaka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Animal Model ◽  
Binding Capacity ◽  
Mammalian Species ◽  
Normal Liver ◽  
Liver Cells ◽  
Pcr Analysis ◽  
Gene Expressions ◽  
Gene Regulations ◽  
Rbc Count

Abstract Recent studies on EPO-EPOR systems in non-mammalian vertebrate including frog and teleost fishes demonstrate extramedullary adult erythropoiesis instead of bone marrow. In adult Xenopus laevis (African clawed frog), an animal model of hepatic erythropoiesis should give an opportunity for understanding the microenvironment of vascular niche. Therefore, we investigate on erythrocyte development, gene expressions and gene regulations of EPO and EPOR molecules in adult Xenopus liver. In situ hybridization and immunostaining revealed that Xenopus EPOR (xlEPOR) expressing erythrocyte progenitors, which have low hemoglobin content, were localized among liver sinusoids. The maximum number of xlEPOR expressing cells was observed when peripheral RBC count reached a nadir after hemolytic anemia. Flowcytometric analysis of peripheral blood cells and dispersed liver cells using anti-xlEPOR antibodies also indicate that the xlEPOR+ cells in liver were increased as decreasing count of peripheral RBC. After a nadir of RBC count, xlEPOR+ immature nucleated erythrocytes were emerged in the circulation. The count of xlEPOR+ immature erythrocytes was gradually decreased as increasing count of xlEPOR− mature erythrocytes. Since mature erythrocytes are still nucleated in Xenopus, we used xlEPOR molecules as erythroid differentiation marker. Meanwhile real time RT-PCR analysis of Xenopus EPO (xlEPO) mRNA revealed that xlEpo gene expression was significantly induced in anemic liver compared to normal liver. These data suggest that xlEPO-xlEPOR signaling between erythrocyte progenitors and liver cells progress the proliferation and differentiation of erythrocyte progenitors, and the mobilization of immature erythrocytes into the circulation. Our previous study showed that the anemic serum of phenylhydrazine administrated Xenopus contains erythroid colony forming activity; however, there is no information about the relationship between anemia and hypoxia enough to stimulate erythropoiesis in adult Xenopus. In mammalian species, Epo gene expression is upregulated by binding of hypoxia inducible factor-1a (HIF-1a) and ARNT complex to hypoxia response element (HRE) located in 3′ enhancer region of Epo gene. In frog and fishes, EPO mRNAs are expressed even in normoxia condition. In fish Epo genes, consensus HRE sequence (ACGTG) were not found in 3′UTR, as well as the reporter assay failed to show Epo upregulation respond to hypoxia. Since any consensus HRE sequence was not found in 3′ UTR of xlEpo gene, we examined whether HIF-1a mediates xlEpo gene regulation. By western blot analysis of HIF-1a, we assessed whether HIF-1a is stabilized in anemia; meanwhile binding capacity of HIF-1a to 5′, 3′ UTRs and intron regions of xlEpo gene was analyzed by gel shift mobility assay. The findings in non-mammalian animal model demonstrate the basis of erythropoietic gene regulations, as well as molecular mechanism underlying adult extramedullary erythropoiesis.

scholarly journals Enhanced regulatory gene expressions in the blood and articular cartilage of patients with rheumatoid arthritis

2012 ◽  
Vol 0 (4) ◽  
pp. 44
Author(s):  
Elena Vasilyevna Chetina ◽  
L A Semyonova ◽  
A L Logunov ◽  
V V Kolomatsky ◽  
M A Makarov ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Articular Cartilage ◽  
Regulatory Gene ◽  
Gene Expressions

scholarly journals Regulatory gene expressions in the ascidian ventral sensory vesicle: evolutionary relationships with the vertebrate hypothalamus

2005 ◽  
Vol 277 (2) ◽  
pp. 567-579 ◽  
Author(s):  
Frédéric Moret ◽  
Lionel Christiaen ◽  
Carole Deyts ◽  
Maryline Blin ◽  
Philippe Vernier ◽  
...  
Keyword(s):  
Regulatory Gene ◽  
Evolutionary Relationships ◽  
Gene Expressions

scholarly journals Carbohydrate-Free Peach (Prunus persica) and Plum (Prunus domestica) Juice Affects Fecal Microbial Ecology in an Obese Animal Model

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e101723 ◽  
Author(s):  
Giuliana D. Noratto ◽  
Jose F. Garcia-Mazcorro ◽  
Melissa Markel ◽  
Hercia S. Martino ◽  
Yasushi Minamoto ◽  
...  
Keyword(s):  
Animal Model ◽  
Microbial Ecology ◽  
Prunus Persica ◽  
Prunus Domestica ◽  
Obese Animal

scholarly journals Gambigyeongsinhwan(1) Improves Body Weight and Lipid Metabolism in High Fat Diet-Fed Obese Animal Model

2014 ◽  
Vol 29 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Soon Shik Shin ◽  
Michung Yoon ◽  
Pei Chin Tsung ◽  
Yong Tae Lee
Keyword(s):  
Animal Model ◽  
Body Weight ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
High Fat ◽  
Obese Animal

scholarly journals Dietary Betaine Addition Alters Carcass Traits, Meat Quality, and Nitrogen Metabolism of Bama Mini-Pigs

2021 ◽  
Vol 8 ◽  
Author(s):  
Yating Cheng ◽  
Mingtong Song ◽  
Qian Zhu ◽  
Md. Abul Kalam Azad ◽  
Qiankun Gao ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Meat Quality ◽  
Fiber Type ◽  
Regulatory Gene ◽  
Carcass Traits ◽  
Animal Husbandry ◽  
Feed Additives ◽  
Carcass Weight ◽  
Gene Expressions ◽  
Mini Pigs

Betaine is widely used as feed additives in animal husbandry as it can cause many benefits such as improving antioxidant ability, growth performance, and carcass traits. However, there are limited studies about the effects of betaine on the Bama mini-pigs. The present study was conducted to evaluate the effects of dietary betaine on carcass traits, meat quality, and nitrogen metabolism of pigs. Twenty-six pregnant Bama mini-pigs and then 104 weaned piglets were assigned for experimental treatments. The plasma and muscle samples were collected at 65-, 95-, and 125-d-old pigs, respectively. The results showed that betaine addition in the sow-offspring diets increased the lean meat rate in the 65-d-old pigs, whereas carcass weight, carcass yield, and loin-eye area were increased in the 95-d-old pigs, and carcass weight and backfat thickness in the 125-d-old pigs. Dietary betaine addition in the sow-offspring diets increased the contents of plasma Asp of 65-d-old, Met of 95- and 125-d-old, and Sar of 125-d-old pigs. Moreover, betaine addition increased the contents of Met, His, Ile, and Phe in Longissimus thoracis et lumborum, whereas those contents were decreased in biceps femoris and psoas major muscles at different stages. Betaine addition in the sow and piglets' diets regulated the muscle fiber-type and myogenic regulatory gene expressions. In summary, betaine addition in the sow and sow-offspring diets could improve the carcass traits and meat quality by altering the plasma biochemical parameters, amino acid composition, and gene expressions of skeletal muscle.

scholarly journals Crmp4-KO Mice as an Animal Model for Investigating Certain Phenotypes of Autism Spectrum Disorders

2019 ◽  
Vol 20 (10) ◽  
pp. 2485 ◽  
Author(s):  
Ritsuko Ohtani-Kaneko
Keyword(s):  
Animal Model ◽  
De Novo ◽  
Current Knowledge ◽  
Autism Spectrum ◽  
Gene Expressions ◽  
Sensory Sensitivities ◽  
Collapsin Response Mediator Protein ◽  
Whole Exome ◽  
Mediator Protein ◽  
Sensory Processing Disorders

Previous research has demonstrated that the collapsin response mediator protein (CRMP) family is involved in the formation of neural networks. A recent whole-exome sequencing study identified a de novo variant (S541Y) of collapsin response mediator protein 4 (CRMP4) in a male patient with autism spectrum disorder (ASD). In addition, Crmp4-knockout (KO) mice show some phenotypes similar to those observed in human patients with ASD. For example, compared with wild-type mice, Crmp4-KO mice exhibit impaired social interaction, abnormal sensory sensitivities, broader distribution of activated (c-Fos expressing) neurons, altered dendritic formation, and aberrant patterns of neural gene expressions, most of which have sex differences. This review summarizes current knowledge regarding the role of CRMP4 during brain development and discusses the possible contribution of CRMP4 deficiencies or abnormalities to the pathogenesis of ASD. Crmp4-KO mice represent an appropriate animal model for investigating the mechanisms underlying some ASD phenotypes, such as impaired social behavior, abnormal sensory sensitivities, and sex-based differences, and other neurodevelopmental disorders associated with sensory processing disorders.

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