Proteomic profile and polyamine contents are modulated by light source to promote in vitro shoot development in Cariniana legalis (Martius) O. Kuntze (Lecythidaceae)

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

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Significance of disorders of proteomic profile of follicular fluid for predicting the effectiveness of in vitro fertilisation in women with infertility

Kazan medical journal ◽

10.17816/kmj2018-496 ◽

2018 ◽

Vol 99 (3) ◽

pp. 496-503

Author(s):

O S Zolotykh ◽

S V Lomteva ◽

K Yu Sagamonova

Keyword(s):

Assisted Reproductive Technology ◽

Follicular Fluid ◽

Molecular Mechanisms ◽

Reproductive Technologies ◽

Reproductive Technology ◽

In Vitro Fertilisation ◽

Proteomic Profile ◽

Technology Programs ◽

Group 2

Aim. To study the proteomic profile of follicular fluid in patients with infertility in assisted reproductive technology programs. Methods. The study included women with infertility included in assisted reproductive technology programs: 15 women who had in vitro fertilisation which resulted in pregnancy (group 1) and 16 women with a negative result of this program (group 2). Fractionation of the follicular fluid samples was performed using the sets of special magnetic beads. Proteomic profiling was performed by tandem MALDI-mass-spectrometry. The anti-Müllerian hormone level was measured by ELISA. Results. The study revealed differences in the detectability of follicular fluid proteins with different regulatory properties in patients of groups 1 and 2. With the negative outcome of in vitro fertilisation, expression of a number of proteins involved in the processes of folliculogenesis, ovulation, selection of the dominant follicle, as well as proteins necessary for the development of the zygote and blastula was reduced in females' follicular fluid. Increased expression in women from group 2 was registered for proteins enhancing proteolytic reactions, cell apoptosis, including oocytes, which disrupt the positive action of activin and damage structural and functional state of mitochondria. A definite relationship was found between the level of anti-Müllerian hormone and rate of detection of a number of proteins, in particular protocadherin-2α, cystatin C, betaglycan, prostatic acid phosphatase, and dermicidin. Conclusion. The revealed changes in proteomic profile of the follicular fluid obviously play an important role in the molecular mechanisms that determine the effectiveness of assisted reproductive technologies; the identified differentially expressed proteins can serve as objective markers for predicting the outcomes of in vitro fertilisation.

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In Vitro Shoot Development of Eucalyptus citriodora on Rockwool in the Film Culture Vessel under CO2 Enrichment

Journal of Forest Research ◽

10.1007/bf02348330 ◽

1996 ◽

Vol 1 (4) ◽

pp. 227-230 ◽

Cited By ~ 13

Author(s):

Shirou Nagae ◽

Takejiro Takamura ◽

Toshiaki Tanabe ◽

Akira Murakami ◽

Kunichika Murakami ◽

...

Keyword(s):

Co2 Enrichment ◽

Shoot Development ◽

Culture Vessel ◽

Film Culture ◽

Eucalyptus Citriodora

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Leucine-Rich Diet Modulates the Metabolomic and Proteomic Profile of Skeletal Muscle during Cancer Cachexia

Cancers ◽

10.3390/cancers12071880 ◽

2020 ◽

Vol 12 (7) ◽

pp. 1880 ◽

Cited By ~ 1

Author(s):

Bread Cruz ◽

André Oliveira ◽

Lais Rosa Viana ◽

Leisa Lopes-Aguiar ◽

Rafael Canevarolo ◽

...

Keyword(s):

Skeletal Muscle ◽

Cancer Cachexia ◽

Energy Generation ◽

In Vitro Assays ◽

Proteomic Profile ◽

Adult Rats ◽

Vitro Assay ◽

Tumour Bearing

Background: Cancer-cachexia induces a variety of metabolic disorders, including skeletal muscle imbalance. Alternative therapy, as nutritional supplementation with leucine, shows a modulatory effect over tumour damage in vivo and in vitro. Method: Adult rats distributed into Control (C), Walker tumour-bearing (W), control fed a leucine-rich diet (L), and tumour-bearing fed a leucine-rich diet (WL) groups had the gastrocnemius muscle metabolomic and proteomic assays performed in parallel to in vitro assays. Results: W group presented an affected muscle metabolomic and proteomic profile mainly related to energy generation and carbohydrates catabolic processes, but leucine-supplemented group (WL) recovered the energy production. In vitro assay showed that cell proliferation, mitochondria number and oxygen consumption were higher under leucine effect than the tumour influence. Muscle proteomics results showed that the main affected cell component was mitochondria, leading to an impacted energy generation, including impairment in proteins of the tricarboxylic cycle and carbohydrates catabolic processes, which were modulated and improved by leucine treatment. Conclusion: In summary, we showed a beneficial effect of leucine upon mitochondria, providing information about the muscle glycolytic pathways used by this amino acid, where it can be associated with the preservation of morphometric parameters and consequent protection against the effects of cachexia.

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In vitro meristem-tip culture and regeneration approaches in Congolese cassava accessions (Manihot esculenta Crantz cv. Boma and cv. Mpelo-Nlongi)

International Journal of Biological Research ◽

10.14419/ijbr.v3i2.5197 ◽

2015 ◽

Vol 3 (2) ◽

pp. 72

Author(s):

J.-Roger Bansimba Mukiese ◽

Aimé Diamuini Ndofunsu ◽

Freddy Bulubulu ◽

Alexandre Mbaya Ntumbula ◽

Sébastien Luyindula Ndiku

Keyword(s):

Growth Regulators ◽

Manihot Esculenta ◽

Callus Formation ◽

Shoot Development ◽

Media Composition ◽

Manihot Esculenta Crantz ◽

Adenine Sulphate ◽

Meristem Tip Culture ◽

The Media

Shiny dome-like structures measuring less than 1mm in length were excised aseptically from shoot tip buds of infected of two cassava (Manihot esculenta Crantz) local cultivars (Boma and Mpelo Nlongi) and cultivated in vitro in two types of media with different combination of growth hormone: Murashige and Skoog supplemented of sucrose (20 g/l), Naphtalenacetic acid (NAA, 10 μM), Ben-zylaminopurine (BAP, 0.66 μM) as well as Gibberellic acid (GA3, 0.1 μM) with 80 mg/l of Adenine sulphate and MS-free growth regulators. After four weeks, data were scored: 29.5% responding explant with callus formation and 20.5% responding explants to shoot development in the medium with growth regulators for the cultivar Boma whereas the cultivar Mpelo-Nlongi presented 5.7% and 25.7% respectively of callus formation and shoot development. The cultivar Boma presented a tendency more pronounced for the callus formation rather than with the shoot development contrary to the cultivar Mpelo-Nlongi. In regards of this experiment, it was shown that the media composition and genotype are essential factors, which influence in vitro growth, mainly the shoot development, in the culture of meristems for cassava local accessions.

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LIGHT QUALITY IN THE IN VITRO INTRODUCTION OF Corymbia HYBRID CLONES

Revista Árvore ◽

10.1590/1806-90882018000600004 ◽

2018 ◽

Vol 42 (6) ◽

Cited By ~ 1

Author(s):

Denys Matheus Santana Costa Souza ◽

Aloisio Xavier ◽

Wagner Campos Otoni ◽

Natane Amaral Miranda ◽

Joane Helena Maggioni

Keyword(s):

Light Source ◽

Culture Medium ◽

Light Quality ◽

Shoot Length ◽

Nodal Segments ◽

Fluorescent Lamp ◽

Corymbia Citriodora ◽

Hybrid Clones ◽

Effect Of Light

ABSTRACT Micropropagation via axillary bud proliferation is recommended for rejuvenation or reinvigoration of selected clones, as well as for improving clonal seedlings rooting. The success of a micropropagation protocol depends on the in vitro introduction, since following phases, multiplication, elongation, and rooting can only take place once the aseptic crop with vegetative vigor has been established. This study aims to assess the effect of light on the in vitro introduction of hybrid clones of Corymbia torelliana x C. citriodora e Corymbia citriodora x C. torelliana by the micropropagation technique through proliferation by axillary buds. The mini-stumps, suppliers of explants for in vitro introduction, were conducted in semi-hydroclonal mini-clonal hedge. Nodal segments from three Corymbia torelliana x C. citriodora (TC01, TC02 e TC03) clones and one Corymbia citriodora x C. torelliana (CT01) clone were collected, disinfested and inoculated in JADS culture medium, in order to compare the effects of light quality from a dark/fluorescent lamp, a fluorescent lamp, and white and red/blue LEDs. At 30 days after inoculation, the following characteristics were evaluated: average contamination percentage, oxidation, non-reactive explants, shoot length and average number of shoots per explant greater than 0.5 cm. Gathered data showed that the use of red/blue LED light source obtained the best results in all assessed characteristics in the in vitro introduction.

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Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha43210036 ◽

2015 ◽

Vol 43 (2) ◽

pp. 542-546 ◽

Cited By ~ 1

Author(s):

Giovanni IAPICHINO ◽

Marcello AIRÒ ◽

Emilio LO PRESTI ◽

Leo SABATINO

Keyword(s):

Acetic Acid ◽

Shoot Proliferation ◽

Shoot Development ◽

In Vitro Rooting ◽

Nodal Segments ◽

Axillary Shoot ◽

High Plasticity ◽

Single Node ◽

Indole 3 Acetic Acid

Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi-hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis. The Murashige and Skoog medium, augmented with different concentrations of N-6-benzyladenine either singly or in combination with indole-3-acetic acid, as potential medium for shoot multiplication by nodal segments was tested. In the following experiment equal molar concentrations of four cytokinins (2-isopenthenyladenine, kinetin, zeatin and N-6-benzyladenine) were tested for ability to induce axillary shoot development from single node stem segments. The highest rate of axillary shoot proliferation was induced on the medium supplemented with 0.44 µM BA. Growth regulator requirements for shoot proliferation in G. aetnensis were satisfied by BA alone. Explants were divided, subcultured and continued to proliferate shoots. A proliferation rate of 3.5 shoots per single node explants every four weeks occurred. Seven indole-3-acetic acid concentrations (0, 0.23, 0.45, 0.91, 1.82, 3.64 or 7.29 µM) were tested to determine the optimum conditions for in vitro rooting of microshoots. The highest rooting percentage was obtained with indole-3-acetic acid at 3.64 mM (57%). Eighty percent of the in vitro rooted plantlets were successfully established in soil. This micropropagation system of G. aetnensis based on axillary shoot development from nodal segments followed by in vitro rooting should be preferred for rapid and efficient mass propagation of selected clones and could represent an alternative method to sexual and conventional asexual propagation.

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