The effects of methyl jasmonate and salicylic acid on the production of secondary metabolites in organ culture of Ziziphora persica

Stevia rebaudiana Bertoni has been promoted for having sweet leaves as well as pharmaceutical and industrial properties. The sweet taste of Stevia leaves is due to the presence of steviol glycosides (a group of diterpene glycosides) found in a small number of plants. In the biosynthetic pathway of steviol glycosides (SGs), 15 enzymes that express the genes are associated with these enzymes under the influence of the elicitors. Due to the individuality of the stevia and few studies on the biosynthesis pathway of SGs, this paper attempted to investigate the effects of some of the elicitors, including methyl jasmonate (MeJA), salicylic acid (SA), auxins (Aux), cytokinins (CKs), gibberellins (GAs) and its inhibitors including paclobutrazol (BPZ) and chloroquate (CCC)), on the responsible genes for the biosynthesis of SGs. Some of these elicitors, including MeJA, SA and GA have great potential in increasing secondary metabolites. Moreover, the biosynthetic pathway of GAs and SGs are shared till ent-kaurenoic acid (ent-KA) biosynthesis, which raises the question of whether this hormone and its inhibitors are effective in the SGs biosynthesis.

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The Accumulation of Essential Oils In Organ Culture of Acinos Graveolens Via Methyl Jasmonate And Salicylic Acid Exertion

10.21203/rs.3.rs-1075365/v1 ◽

2021 ◽

Author(s):

Rouhollah Motafakkerazad ◽

Vahideh Soltani-Elired ◽

Sarieh Tarigholizadeh ◽

Morteza Kosari-Nasab

Keyword(s):

Salicylic Acid ◽

Essential Oil ◽

Organ Culture ◽

Methyl Jasmonate ◽

Growth Parameters ◽

Nodal Segments ◽

Chl A ◽

Quantitative Changes ◽

Almost All ◽

The Impact

Abstract The impact of Salicylic acid (SA) and Methyl jasmonate (MJ) on growth, and essential oil quantity and quality in organ culture of Acinos graveolens were investigated. In the present study, nodal segments were used as explants for shoot production using MS media with BA and NAA. Afterward, they were transferred to liquid MS media culture containing SA and MJ with 50, 100, and 150 µM and their combinations (SA25+MJ25, SA50+MJ25, SA25+MJ50, and SA50+MJ50). After three weeks, samples were collected to assess the morphological and some growth parameters, quantitative changes in pigment amounts as well as volatile contents. Accordingly, almost all treatments led to a notable decrease in growth parameters in comparison with control. The highest amount of Chl a, b and carotenoids were achieved by MJ100, SA50, and SA25+MJ50, respectively. In addition, GC-MS results indicated 38 components of volatile products mainly alkane hydrocarbons and sesquiterpenes. The major identified compounds were Decane, Eicosane, and Germacrene D. Altogether, results asserted that SA25+MJ25 and SA100 were more efficient in the enhancement of essential oil content among all treatments.

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Cloning and Characterization of Promoters of the Fungal Immunomodulatory Protein Genes from Ganoderma spp. (Agaricomycetes) and Their Response to Methyl Jasmonate and Salicylic Acid

International Journal of Medicinal Mushrooms ◽

10.1615/intjmedmushrooms.2018025451 ◽

2018 ◽

Vol 20 (2) ◽

pp. 177-189 ◽

Cited By ~ 1

Author(s):

Qi-Zhang Li ◽

Yu-Zhou Chang ◽

Kai-Qi Su ◽

Xiao-Lei Wang ◽

Xiao-Hui Bai ◽

...

Keyword(s):

Salicylic Acid ◽

Methyl Jasmonate ◽

Fungal Immunomodulatory Protein ◽

Immunomodulatory Protein

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Cultivar-Specific Changes in Primary and Secondary Metabolites in Pak Choi (Brassica Rapa, Chinensis Group) by Methyl Jasmonate

International Journal of Molecular Sciences ◽

10.3390/ijms18051004 ◽

2017 ◽

Vol 18 (5) ◽

pp. 1004 ◽

Cited By ~ 13

Author(s):

Moo Kim ◽

Yu-Chun Chiu ◽

Na Kim ◽

Hye Park ◽

Choong Lee ◽

...

Keyword(s):

Secondary Metabolites ◽

Methyl Jasmonate ◽

Brassica Rapa ◽

Pak Choi ◽

Primary And Secondary Metabolites

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Effect of Methyl Jasmonate, Salicylic Acid and Their Combination on Growth and Bioactive Constituents in Cichoriun intybus L. تأثير ميثايل حامض الجاسمونيک و حامض السالسيلک و مخلوطهما معآ على النمو و المحتوى من مرکبات التمثيل الثانوى ذات النشاط الحيوى فى نبات الشيکوريا Cichoriun intybus L.

Journal of Plant Production ◽

10.21608/jpp.2021.187442 ◽

2021 ◽

Vol 12 (7) ◽

pp. 747-755

Author(s):

Heba Ibrahim

Keyword(s):

Salicylic Acid ◽

Methyl Jasmonate ◽

Bioactive Constituents

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Alkaloids Production and Cell Growth of Cinchona ledgeriana Moens: Effects of Fungal Filtrate and Methyl Jasmonate Elicitors

Indonesian Journal of Science and Technology ◽

10.17509/ijost.v6i1.31479 ◽

2021 ◽

Vol 6 (1) ◽

pp. 31-40

Author(s):

Yustiny Andaliza Hasibuan ◽

Diah Ratnadewi ◽

Zainal Alim Mas’ud

Keyword(s):

Cell Culture ◽

Cell Growth ◽

Secondary Metabolites ◽

Methyl Jasmonate ◽

Woody Plant ◽

Cultured Cells ◽

Cinchona Alkaloids ◽

Cell Biomass ◽

Cinchona Ledgeriana ◽

Culture Technology

Cinchona alkaloids are known as antimalaria and anti-arrhythmic. Due to the long waiting time to harvest, cell culture technology is a challenge. This study aimed to determine the effects of elicitors, filtrate of two strains of endophytic fungi and methyl jasmonate (MeJA), in cell suspension culture of Cinchona ledgeriana on quinine and quinidine production. The cells were cultured for seven weeks in woody plant (WP) media treated with either of those elicitors in various concentrations. The cells growth was observed and the alkaloids were analyzed by HPLC. Cells treated with MeJA failed to grow that led to the cell biomass insufficiency for alkaloids determination. It indicates that the cells are quite sensitive to even low concentration of MeJA that hampered the growth. Cells treated with the filtrate of Diaporthe sp. M13-Millipore filtered (S2M) gave the least cell biomass but presented the highest content of both alkaloids. Diaporthe sp. strain M-13 is stronger as elicitor than M-23 for this plant species. Filtrate of non-virulent fungi can elevate the biosynthesis of alkaloids. This reconfirms that cultured cells are capable to produce secondary metabolites and the productivity can be increased by using an appropriate elicitor.

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