Assessing the Potential of Organic Solvents on Total Petroleum Hydrocarbon Extraction from Diesel-Contaminated Soils

2017 ◽  
Vol 228 (5) ◽  
Author(s):  
Man Jae Kwon ◽  
Yunho Hwang
Author(s):  
Williams, Janet Olufunmilayo ◽  
Aleruchi Owhonka

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l.  The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.


2021 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Teddy Irawan ◽  
Bintal Amin ◽  
Sofia Anita

The increasing need for fuel derived from petroleum is also in line with the increased exploration and production activities of petroleum. Besides producing crude oil products, this activity also produces petroleum waste. This waste will pollute the soil which will have an impact on environmental damage, disruption of human health and other living things. Therefore according to Veegha (2008), an efficient and environmentally friendly method for treating petroleum waste is needed. One of the waste treatment methods is using phytoremediation method. Phytoremediation is defined as a technology for cleaning, removing or reducing harmful pollutants, such as heavy metals, pesticides, and toxic organic compounds in soil or water using the help of plants. This research was conducted for three months from September to November 2019 at the open nursery of PT. CPI with paranet shade 60%. This study aims to determine the effectiveness of bahia grass (P. notatum) in degrading Total Petroleum Hydrocarbon (TPH) in petroleum-contaminated soils in the Rokan Block PT. Chevron Pacific Indonesia and analyze the socio-economic impact of phytoremediation on local communities. The results showed that the effectiveness of Bahia (P. notatum) grass proved effective in reducing the concentration of Total Petroleum Hydrocarbon (TPH) in petroleum-contaminated soils in the Rokan Block operating area of PT. Chevron Pacific Indonesia. The percentage of effectiveness shows a decrease in value of up to 58.38%. The socio-economic impact of phytoremediation on local communities has a positive impact with the planned phytoremediation activities using bahia grass with the community as work agents for planting the grass.


2021 ◽  
Author(s):  
Samuel Beal ◽  
Ashley Mossell ◽  
Jay Clausen

The study objectives were to determine the effectiveness of Fenton’s Reagent and Modified Fenton’s Reagent in reducing Total Petroleum Hydrocarbon (TPH) concentrations in petroleum-contaminated soil from McMurdo Station, Antarctica. Comparisons of the contaminated soils were made, and a treatability study was completed and documented. This material was presented at the Association for Environmental Health and Sciences Foundation (AEHS) 30th Annual International Conference on Soil, Water, Energy, and Air (Virtual) on March 25, 2021.


2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
J. A. Ruley ◽  
A. Amoding ◽  
J. B. Tumuhairwe ◽  
T. A. Basamba ◽  
E. Opolot ◽  
...  

Phytoremediation of hydrocarbon-contaminated soils is a challenging process. In an effort to enhance phytoremediation, soil was artificially contaminated with known concentration of light crude oil containing Total petroleum hydrocarbon (TPH) at a concentration of 75 gkg−1 soil. The contaminated soil was subjected to phytoremediation trial using four plant species (Oryza longistaminata, Sorghum arundinaceum, Tithonia diversifolia, and Hyparrhenia rufa) plus no plant used as control for natural attenuation. These phytoremediators were amended with concentrations (0, 5 and 10 gkg−1 soil) of organic manure (cow dung). Results at 120 days after planting, showed that application of manure at concentrations of 5 and 10 gkg−1 soil combined with an efficient phytoremediator can significantly enhance reduction of TPH compared to natural attenuation or use of either manure or a phytoremediator alone (p<0.05). The study also showed that a treatment combination of manure 5 gkg−1 soil, with a phytoremediator gives a similar mean percentage reduction of TPH as manure 10 gkg−1 soil (p>0.05). Therefore, the study concludes that use of phytoremediators and manure 5 gkg−1 soil could promote the restoration of TPH contaminated-soils in the Sudd region of South Sudan.


Author(s):  
B. M. Popoola ◽  
A. A. Olanbiwonninu

Biodegradation of hydrocarbons by microorganisms represents one of the primary mechanisms by which petroleum and other hydrogen pollutants are eliminated from the environment. This work was carried out on the effect of microorganisms on the biotreatment of oil in crude oil contaminated soil. Microorganisms were isolated from two experimental soil samples contaminated with Bonny Crude and normal uncontaminated soil as a control over a period of seven months. The microbial as well as the physico-chemical parameters of the soil samples were all analyzed using standard methods. Changes in total petroleum hydrocarbon level were measured appropriately. Treatments used were the microbial isolates. Forty-four microorganisms were isolated from the contaminated soils and identified as species of Pseudomonas (7), Flavobacterium (6), Bacillus (8), Proteus (4), Klebsiella (1), Pencillium (5), Aspergillus (7), Fusarium (3), Trichypton (2) and Neurospora (1). Ten of the forty-four isolates had ability to degrade crude oil in the laboratory. On contamination a value of 1.0X105 cfu/g in microbial counts were obtained followed by a subsequent increase in population levels after a period of 2months with a value of 1.0X106 cfu/g. Oil application to the soil resulted in an increase in total petroleum hydrocarbon from 0.31 ppm to 5.53 ppm; organic matter from 0.41% to 7.34%; available phosphorus from 1.75 ppm to 2.84 ppm. The treatment measures all showed progressive decrease in oil concentration in the soil. Mixture of bacterial and fungal isolates as a treatment measure proved to be more favourable above all others, it brought the concentration from 5.53 ppm to 0.31 ppm after a period of 5 weeks of treatment, which is same value with the normal soil (uncontaminated). Species of Pseudomonas, Bacillus, Flavobacterium, Proteus, Klebsiella, Penicillium, Aspergillus, Fusarium, Trichyphyton and Neurospora had potential for the degradation of bonny crude oil. They could therefore be employed in environmental cleanup of petroleum spill site.


Author(s):  
O. Ule ◽  
D. N. Ogbonna ◽  
R. N. Okparanma ◽  
R. R. Nrior

Aim: To assess the Mycoremediation potential of Mucor racemosus and Aspergillus niger in open field crude oil contaminated soils in Rivers State, Nigeria.  Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: Rivers State University demonstration farmland in Nkpolu-Oroworukwo, Mile 3 Diobu area of Port Harcourt, was used for this study. The piece of land is situated at Longitude 4°48’18.50” N and Latitude 6ᵒ58’39.12” E measuring 5.4864 m x 5.1816 m with a total area of 28.4283 square meter. Mycoremediation process monitoring lasted for 56 days, analyses were carried out weekly at 7 days’ interval. Methodology: Five (5) experimental plots were employed using a Randomized Block Design each having dimensions of 100 x 50 x 30 cm (Length x Breadth x Height) and were formed and mapped out on agricultural soil, each plot was contaminated with 22122.25g of Crude Oil except Control 1 and left fallow for 6 days after contamination for proper contamination and exposure to natural environmental factors to mimic crude oil spill site. On the seventh day bio-augmentation process commenced using two (2) fungal isolates namely Aspergillus niger [Asp] and Mucor rasemosus [Muc]). Two (2) control plots (P1: Uncontaminated and unamended soil - CTRL 1 US) and P2: Crude Oil contaminated but unamended soil - CTRL 2 CS); P3 = P5 were contaminated and amended/bioaugmented (P3: CS+Asp, P4: CS+Muc, P5: CS+Asp+Muc respectively. Soil profile before and after contamination was assayed while parameters like Temperature, pH, Nitrogen, Phosphorus, Potassium and Total Petroleum Hydrocarbon (TPH) contents were monitored throughout the experimental period. Microbial analyses such as Total Heterotrophic Bacteria (THB), Total Heterotrophic Fungi (THF), Hydrocarbon Utilizing Bacteria (HUB) and Hydrocarbon Utilizing Fungi (HUF) were recorded. Bioremediation efficiency was estimated from percentage (%) reduction of Total Petroleum Hydrocarbon (TPH) from day 1 to the residual hydrocarbon at day 56 of bio- augmentation/ biostimulation plots with the control. Results: Results revealed actual amount of remediated hydrocarbon and % Bioremediation Efficiency at 56 days in the different treatment plots (initial TPH contamination value of 8729.00mg/kg) in a decreasing order as follows: CS+Muc (8599.19mg/kg; 33.66%) > CS+Asp+Muc (8357.31mg/kg; 33.04%) > CS+Asp (8341.58mg/kg; 32.98%) > CTRL 2 -CS (Polluted soil without amendment) (81.06mg/kg; 0.32%). Microbiological results After fifty-six (56) days of bioremediation monitoring; %HUB were as follows; CS+Asp+Muc (45.30%) > CS+Asp (40.32%) > CS+Muc (35.01%) > CTRL 2 –CS (30.43%) > CTRL 1 – US (0%). These results indicate that the presence of the contaminated crude oil stimulated and sustained the growth of Hydrocarbon Utilizing Bacteria (HUB) in the contaminated plots (P2 - P3); more so, the higher growth in the enhanced bio-augmented plots (P3 – P5) shows the positive impact of fungal bio-augmentation in bioremediation of crude oil polluted soil. It was further observed that treatment plots with higher HUB or HUF had higher percentage (%) bioremediation efficiency; that is, the higher the sustained HUB and HUF population, the higher the %Bioremediation process. Hydrocarbon Utilizing Bacteria (Log10 CFU/g): CS+Asp (4.20) (Day 35) > CS+Muc+Asp (4.18) (Day 35) > CS+Muc (4.08) (Day 28) > CTRL 2 – CS (3.95) (Day 21) > CTRL 1 – US (3.78) (Day 35). (Fig. 3). Hydrocarbon Utilizing Fungi (Log10 CFU/g): CS+Asp (4.68) (Day 35) > CS+Muc+Asp (4.58) (Day 35) > CS+Muc (4.48) (Day 35) > CTRL 2 – CS (4.23) (Day 21) > CTRL 1 – US (2.85) (Day 42). Conclusion: Study showed that bioremediation of crude oil-contaminated soils with Bioaugmenting fungus singly may be more effective than combination with others depending on the type of substrate used, nature of the hydrocarbon utilizing organism and environmental conditions prevalent as seen in Mucor racemosus having higher bioremediation potential than when combined with Aspergillus niger. Notably, Hydrocarbon Utlilizing Bacteria (HUB) and Hydrocarbon Utilizing Fungi (HUF) which are the key players in Bioremediation has its peak count value on Day 35, this confers that nutrient renewal on bioremediation site should be at interval of 35 days for continuous effective bioremediation of hydrocarbon pollutants. It is therefore recommended that single microbes of high bioremediation potential could be used since its more effective than consortium of many hydrocarbon utilizing microbes. Also, nutrient or bio-augmenting microbes’ renewal on bioremediation site should be at an interval of 35 days for continuous effective bioremediation of hydrocarbon pollutants.


2018 ◽  
Vol 40 (1) ◽  
pp. 25-32
Author(s):  
Cut Nanda Sari ◽  
Lina Lubnah

Crude oils processing into energy continue to increase, hence treatment for its environmental impact is needed. The objectives of the study is to determine the differences in bacteria growth rate and removal efficiency of Total Petroleum Hydrocarbon (TPH) between compost and WWTP (Waste Water Treatment Plant) sludge addition at 5% and 10% concentration levels. Those effects were acknowledged through experiments in laboratory scale using soil contaminated by 5,5% TPH within 5 weeks until it reach less than 1% as the requirement. The soil comes from Marunda Beach, compost from UPS Merdeka, WWTP sludge from Jababeka, and bacteria isolated from soil contaminated in the area surrounding refining. The treatment used in this experiment was landfarming with nutrition addition and the main variable analyzed was TPH and the microorganism population. Results of this study show that the bacteria growth rate in compost and WWTP sludge at 5% and 10% concentration each are 0,7567/weeks and 1,154/week for compost and also 0,8783/week and 1,1109/week for WWTP sludge. The TPH removal efficiency obtained was 95,32% and 96,85% for the addition of compsot as well as 91,15% and 91,02% for the addition of WWTP sludge at 5% and 10% concentrations. Base on a t-Test, the differences between all the variation of concentrations are not significant. The correlation test between TPH degradation to bacteria growth showed that there is a weak downward (negative) linear relationship.Kegiatan pengelolaan minyak bumi terus meningkat, maka dari itu dibutuhkan tindakan penanganan pemulihan kondisi lingkungan yang disebabkan oleh kegiatan tersebut. Penelitian ini bertujuan untuk mengetahui perbedaan laju pertumbuhan bakteri dan efi siensi penyisihan Total Petroleum Hydrocarbon (TPH) dengan variasi perlakuan pemberian kompos dan lumpur residu pengelolaan air limbah. Penelitian dilakukan dalam skala laboratorium dengan konsentrasi awal TPH sebesar 5,5% selama 5 minggu atau sampai TPH mencapai konsentrasi kurang dari 1% sesuai dengan baku mutu. Tanah yang digunakan berasal dari Pantai Marunda, Bekasi. Kompos berasal dari UPS (Unit Pengolah Sampah) Merdeka, Depok. Lumpur Instalasi Pengelolaan Air Limbah (IPAL) berasal dari Jababeka. Isolat bakteri yang digunakan berasal dari tanah tercemar TPH disekitar ,kilang minyak. Hasil dari penelitian menunjukkan laju pertumbuhan bakteri pada perlakuan penambahan kompos dan lumpur IPAL pada konsentrasi 5% dan 10% masing-masing adalah 0,7567/minggu dan 1,154/minggu untuk kompos, serta 0,8783/minggu dan1,1109/minggu untuk residu lumpur IPAL. Efisiensi penyisihan TPH yang diperoleh adalah 95,32% and 96,85% untuk penambahan kompos dan 91,15% dan 91,02% untuk penambahan residu lumpur IPAL pada konsentrasi 5% dan 10%. Berdasarkan hasil uji-t, perbedaan untuk masing-masing perlakuan tidaklah signifikan. Uji korelasi antara perubahan konsentrasi TPH dengan pertumbuhan bakteri menunjukkan hubungan lemah berbanding terbalik.


2021 ◽  
Vol 11 (2) ◽  
pp. 781
Author(s):  
Rishikesh Bajagain ◽  
Prakash Gautam ◽  
Seung-Woo Jeong

Surface foam spraying technologies, employing natural infiltration processes, have recently been suggested to not disturb or mix contaminated soils. However, effective delivery of reactive remedial agents to the bottom area of a contaminated region using only natural infiltration processes can be a challenge. This study aimed to improve the delivery of remedial agents such as oxidants, microorganisms, and nutrients to all depths of 30 cm thick unsaturated diesel-contaminated soil using small vertical soil holes. Three vertical holes, occupying 0.8% of the total soil volume and 3% of the soil surface area, were made inside the 17.3 kg soil column. Persulfate oxidation foam and subsequent bioaugmentation foam spraying were applied for remediation of contaminated soil. Foam spraying with vertical soil holes improved the uniformity of distribution of remedial agents throughout the soil, as evidenced by the uniform pH, higher volumetric soil water content, and a microbial population of >107 CFU/g. Therefore, the total petroleum hydrocarbon (TPH) removal efficiency (88–90%) from bottom soils was enhanced compared to soil columns without holes (72–73%) and the control test (5–9%). The kinetic study revealed that relatively similar TPH biodegradation rates (0.054–0.057 d−1) can be obtained for all soil depths by using this new and simple approach.


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