The metabolome of human placental tissue: investigation of first trimester tissue and changes related to preeclampsia in late pregnancy

The mechanism for decreased insulin sensitivity in pregnant women is not fully clarified. Resistin, a novel peptide hormone, is specifically expressed in the adipose tissue and decreases insulin sensitivity in rodents. In the present study, we demonstrate resistin gene expression in the human placental tissue, mainly in trophoblastic cells. The resistin gene expression in term placental tissue was more prominent than was seen in the first trimester chorionic tissue. In contrast resistin gene expression in adipose tissue was rather weak and remained unchanged by pregnancy. Thus, resistin is a newly isolated placental hormone in humans which may modulate insulin sensitivity during pregnancy.

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A Preliminary Study on Human Placental Tissue Impaired by Gestational Diabetes: A Comparison of Gel-Based versus Gel-Free Proteomics Approaches

European Journal of Mass Spectrometry ◽

10.1255/ejms.1412 ◽

2016 ◽

Vol 22 (2) ◽

pp. 71-82 ◽

Cited By ~ 19

Author(s):

Marco Roverso ◽

Maura Brioschi ◽

Cristina Banfi ◽

Silvia Visentin ◽

Silvia Burlina ◽

...

Keyword(s):

Gestational Diabetes ◽

Placental Tissue ◽

Human Placental Tissue ◽

Preliminary Study

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Metabolic Changes in Human Placental Tissue in Diabetes Mellitus

Fetal Nutrition, Metabolism, and Immunology ◽

10.1007/978-1-4684-1191-1_17 ◽

1984 ◽

pp. 209-221

Author(s):

Yoram Z. Diamant ◽

Rita Kissilevitz

Keyword(s):

Diabetes Mellitus ◽

Placental Tissue ◽

Metabolic Changes ◽

Human Placental Tissue

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Effect of glucose and oxygen deprivation on heme oxygenase expression in human chorionic villi explants and immortalized trophoblast cells

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00234.2003 ◽

2003 ◽

Vol 285 (6) ◽

pp. R1453-R1460 ◽

Cited By ~ 11

Author(s):

S. D. Appleton ◽

G. E. Lash ◽

G. S. Marks ◽

K. Nakatsu ◽

J. F. Brien ◽

...

Keyword(s):

Enzymatic Activity ◽

Protein Content ◽

Heme Oxygenase ◽

Glucose Concentration ◽

Placental Tissue ◽

Glucose Deprivation ◽

First Trimester ◽

Trophoblast Cells ◽

Chorionic Villi ◽

Low Glucose

Although hypoxia induces heme oxygenase (HO)-1 mRNA and protein expression in many cell types, recent studies in our laboratory using human placental tissue have shown that a preexposure to hypoxia does not affect subsequent HO enzymatic activity for optimized assay conditions (20% O2; 0.5 mM NADPH; 25 μM methemalbumin) or HO-1 protein content. One of the consequences of impaired blood flow is glucose deprivation, which has been shown to be an inducer of HO-1 expression in HepG2 hepatoma cells. The objective of the present study was to test the effects of a 24-h preexposure to glucose-deprived medium, in 0.5 or 20% O2, on HO protein content and enzymatic activity in isolated chorionic villi and immortalized HTR-8/SVneo first-trimester trophoblast cells. HO protein content was determined by Western blot analysis, and microsomal HO enzymatic activity was measured by assessment of the rate of CO formation. HO enzymatic activity was increased ( P < 0.05) in both placental models after 24-h preexposure to glucose-deficient medium in 0.5 or 20% O2. Preexposure (24 h) in a combination of low O2 and low glucose concentrations decreased the protein content of the HO-1 isoform by 59.6% ( P < 0.05), whereas preexposure (24 h) to low glucose concentration alone increased HO-2 content by 28.2% in chorionic villi explants ( P < 0.05). In this preparation, HO enzymatic activity correlated with HO-2 protein content ( r = 0.825). However, there was no correlation between HO-2 protein content and HO enzymatic activity in HTR-8/SVneo trophoblast cells preexposed to 0.5% O2 and low glucose concentration for 24 h. These findings indicate that the regulation of HO expression in the human placenta is a complex process that depends, at least in part, on local glucose and oxygen concentrations.

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Seasonally Variant Gene Expression in Full-Term Human Placenta

10.1101/2020.01.27.20018671 ◽

2020 ◽

Author(s):

Danielle A. Clarkson-Townsend ◽

Elizabeth Kennedy ◽

Todd M. Everson ◽

Maya A. Deyssenroth ◽

Amber A. Burt ◽

...

Keyword(s):

Health Outcomes ◽

Placental Tissue ◽

Later Life ◽

Full Term ◽

Season Of Birth ◽

Rhythmic Expression ◽

Health And Development ◽

Human Placental Tissue ◽

Variant Genes ◽

Ribosomal Activity

ABSTRACTSeasonal exposures influence human health and development. The placenta, as a mediator of the maternal and fetal systems and a regulator of development, is an ideal tissue to understand the biological pathways underlying relationships between season of birth and later life health outcomes. Here, we conducted a transcriptome-wide association study of season of birth in full-term human placental tissue to evaluate whether the placenta may be influenced by seasonal cues. Of the analyzed transcripts, 583 displayed differential expression between summer and winter births (FDR q<0.05); among these, BHLHE40, MIR210HG, and HILPDA had increased expression among winter births (Bonferroni p<0.05). Enrichment analyses of the seasonally variant genes between summer and winter births indicated over-representation of transcription factors HIF1A, VDR, and CLOCK, among others, and of GO term pathways related to ribosomal activity and infection. Additionally, a cosinor analysis found rhythmic expression for approximately 11.9% of all 17,664 analyzed placental transcripts. These results suggest that the placenta responds to seasonal cues and add to the growing body of evidence that the placenta acts as a peripheral clock, which may provide a molecular explanation for the extensive associations between season of birth and health outcomes.

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