scholarly journals Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

2021 ◽  
Author(s):  
Gustaf MO Ekelund Ugge ◽  
Annie Jonsson ◽  
Olof Berglund
Keyword(s):  
Industrial Wastewater ◽  
Freshwater Mussel ◽  
Effect Sizes ◽  
Control Treatment ◽  
Treatment Results ◽  
Toxic Unit ◽  
Molecular Biomarker ◽  
Order Of Magnitude ◽  
Digestive Glands ◽  
Selection Of

AbstractUsing a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit estimates were used as proxies of chemical stress, and Cu, Ni, and Zn were identified as potential major contributors (Cu> Ni > Zn). Six transcriptional (cat, gst, hsp70, hsp90, mt, sod) and two biochemical (AChE, GST) biomarkers were analyzed in two tissues, gills, and digestive glands. Out of the 16 responses (eight biomarkers × two tissues), 14 effect sizes were small (within ± 28 % of control) and differences non-significant (p > 0.05). Results did however show that (1) AChE activity increased by 40% in gills of exposed mussels compared to control, (2) hsp90 expression was 100% higher in exposed female gills compared to control, and (3) three marker signals (AChE in both tissues, and hsp70 in gills) differed between sexes, independent of treatment. Results highlight a need for further investigation of molecular biomarker variability and robustness in A. anatina.

Context in Selection of Men and Women in Hiring Decisions: Gender Composition of the Applicant Pool

2005 ◽  
Vol 96 (2) ◽  
pp. 349-360 ◽  
Author(s):  
Jos Van Ommeren ◽  
Giovanni Russo ◽  
Reinout E. De Vries ◽  
Mark Van Ommeren
Keyword(s):  
The Netherlands ◽  
Job Applicants ◽  
Effect Sizes ◽  
Gender Composition ◽  
Lower Probability ◽  
Hiring Decisions ◽  
Sex Composition ◽  
Applicant Pool ◽  
Men And Women ◽  
Selection Of

The hypothesis that the sex composition of an applicant pool affects the hiring probabilities of individual job applicants was tested using gender-distinctive information on accepted and rejected job applicants in The Netherlands. The evidence supports this hypothesis, although the effect sizes are moderate. Both men and women have a lower probability of being hired when the applicant pool contains fewer applicants from their own sex.

scholarly journals Production of Synseed for Hybrid Cymbidium Using Protocorm-Like Bodies

2012 ◽  
Vol 20 (2) ◽  
pp. 135-146 ◽  
Author(s):  
Jaime A. Teixeira da Silva
Keyword(s):  
Coconut Water ◽  
Control Treatment ◽  
Synthetic Seed ◽  
Naphthaleneacetic Acid ◽  
Short Term ◽  
C Storage ◽  
Cell Layers ◽  
Dark Culture ◽  
Selection Of

Abstract Synthetic seed were produced from protocorm-like bodies (PLBs) of hybrid Cymbidium Twilight Moon ‘Day Light’ after culture on a new medium, Teixeira Cymbidium (TC) medium. This new medium contained, in addition to a unique selection of macro- and micronutrients, 0.1 mg/l α-naphthaleneacetic acid and 0.1 mg/l kinetin, 2 g/l tryptone and 20 g/l sucrose, and was solidified with 8 g/l Bacto agar. Several explant types and sizes (intact PLBs, half-PLBs, PLB longitudinal thin cell layers) were tested. In addition, pretreatment of PLB-synseeds with 200 mM KNO3 solution, the addition of activated charcoal or coconut water to synseeds, light vs dark culture, short-term (1 month) and long-term (6 and 12 months) low-temperature (4°C) storage, as well as cryostorage were also tested. All treatments resulted in less PLBs than the control treatment. Among all these treatments, only the use of TC medium or incorporation of coconut water into synseeds resulted in “germination” while lowtemperature storage (1-6 months) was only possible under liquid TC. These results would allow for the short-term preservation of Cymbidium germplasm but not for effective cryopreservation.

How effective is KRM-1648 in treatment of disseminated Mycobacterium avium complex infections in beige mice?

1996 ◽  
Vol 40 (2) ◽  
pp. 437-442 ◽  
Author(s):  
B Ji ◽  
N Lounis ◽  
C Truffot-Pernot ◽  
J Grosset
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Complex ◽  
Antimicrobial Effect ◽  
Bacteriostatic Effect ◽  
Dose Selection ◽  
Order Of Magnitude ◽  
The Mean ◽  
Resistant Mutants ◽  
Selection Of

Although the MICs of 3'-hydroxy-5'-(4-isobutyl-1-piperazinyl)benzoxazinorifamycin, or KRM-1648 (KRM), for Mycobacterium avium complex (MAC) were significantly lower than those of other drugs, its in vivo activity was very weak. Beginning 28 days after inoculation, beige mice that had been infected intravenously with 1.87 x 10(7) CFU of MAC 101 were administered KRM alone, clarithromycin (CLARI) alone, or CLARI plus KRM six times weekly for 16 weeks. In contrast to the mice treated with CLARI-containing regimens, the mortality and the mean spleen weights of mice treated with KRM alone (either 10 or 20 mg/kg of body weight per dose) did not differ significantly from those of untreated mice, their numbers of CFU were very much greater than pretreatment values, and multiplication of MAC was only slightly inhibited. Although monotherapy by KRM selected KRM-resistant mutants, the selection was very weak; the mean number of CFU and the frequency of KRM-resistant mutants increased by no more than 1 order of magnitude after 16 weeks of treatment with KRM at 20 mg/kg per dose. Selection of CLARI-resistant mutants was inhibited but not completely prevented by treatment of the mice with CLARI plus KRM. These results indicate that KRM displayed only a weak bacteriostatic effect against the isolate tested in the beige mouse model; its ability to enhance the antimicrobial effect of CLARI or to prevent emergence of CLARI-resistant mutants was very limited.

Locally Available Aggregate and Sediment Production

2003 ◽  
Vol 1819 (1) ◽  
pp. 185-193 ◽  
Author(s):  
Randy B. Foltz ◽  
Mark Truebe
Keyword(s):  
Road Construction ◽  
Forest Road ◽  
Sediment Production ◽  
Magnitude Range ◽  
Long Duration ◽  
Order Of Magnitude ◽  
Equivalent Test ◽  
Basic Characteristics ◽  
Material Tests ◽  
Selection Of

Selection of suitable locally available materials to build strong and durable roads with aggregate surfaces is desired to minimize road construction and maintenance costs and to minimize the detrimental effects of sedimentation. Eighteen aggregates were selected from local sources in Idaho, Oregon, South Dakota, and Washington State. Aggregate was placed in shallow metal frames and compacted to simulate a forest road. The levels of runoff and sediment from a highintensity, long-duration simulated rainstorm were measured. The material tests selected for use in the study included ones that define the basic characteristics of the aggregate, along with a number of tests intended to predict susceptibility to erosion. Each of the tests was statistically evaluated to identify those that best predicted the perceived aggregate quality. The two best indicators of aggregate quality were the results of the sand equivalent test and the P20 portion of the Oregon air degradation test. The best indicator of either runoff or sediment production was the fraction passing the 0.6-mm sieve. Acceptable aggregates, both those of good quality and those of marginal quality, exhibited a 2-order-of-magnitude range in both runoff and sediment production.

scholarly journals Sensorial Properties, Chemical Characteristicsand Fatty Acids Profile of Cheese Fortified by Encapsulated Kilka Fish Oil

2016 ◽  
Vol 10 (3) ◽  
pp. 208 ◽  
Author(s):  
SeyedReza Hejazian ◽  
SeyedehZeynab Hatami Takami ◽  
Esmaeil Ghanbari Shendi
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Fish Oil ◽  
Point Of View ◽  
Poultry Feed ◽  
Control Treatment ◽  
Treatment Results ◽  
Fatty Acids Profile ◽  
Significant Difference ◽  
Spray Dried

<p>However Kilka is a valuable fish in nutritional point of view, but a large part of it used in poultry feed. The main<br />reason is the undesirable odor. In this study Kilka oil was blended with milk at 1% and 2% level and then the<br />mixture spray dried. These encapsulated Kilka oil were added to cheese as a fortificant materials at 5% level.<br />Cheese without encapsulated Kilka oil was as a control treatment. Results showed that there was no significant<br />difference (p&gt;0.05) between color of fortificated cheese with control cheese. There was no significant difference<br />(p&gt;0.05) between odor of cheese with 5% encapsulated Kilka oil that contain 1% Kilka oil (A) with control<br />cheese. There was no significant difference (p&gt;0.05) between flavor of cheese with 5% encapsulated Kilka oil<br />that contain 1% Kilka oil (A) with cheese with 5% encapsulated Kilka oil that contain 2% Kilka oil (B) but there<br />was significant difference (p&lt;0.05) between these two treatments with control cheese. Also, the eicosapentaenoic<br />acid (EPA) and docosahexaenoic acid (DHA) content of fortified cheeses had significant difference (p&lt;0.05)<br />with control cheese.</p>

scholarly journals Selection for antibiotic resistance is reduced when embedded in a natural microbial community

2019 ◽  
Author(s):  
Uli Klümper ◽  
Mario Recker ◽  
Lihong Zhang ◽  
Xiaole Yin ◽  
Tong Zhang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Community ◽  
Single Species ◽  
Natural Environments ◽  
Order Of Magnitude ◽  
Natural Microbial Community ◽  
Cost Of Resistance ◽  
Selection For ◽  
The Cost ◽  
Selection Of

AbstractAntibiotic resistance has emerged as one of the most pressing, global threats to public health. In single-species experiments selection for antibiotic resistance occurs at very low antibiotic concentrations. However, it is unclear how far these findings can be extrapolated to natural environments, where species are embedded within complex communities. We competed isogenic strains of Escherichia coli, differing exclusively in a single chromosomal resistance determinant, in the presence and absence of a pig fecal microbial community across a gradient of antibiotic concentration for two relevant antibiotics: gentamicin and kanamycin. We show that the minimal selective concentration was increased by more than one order of magnitude for both antibiotics when embedded in the community. We identified two general mechanisms were responsible for the increase in minimal selective concentration: an increase in the cost of resistance and a protective effect of the community for the susceptible phenotype. These findings have implications for our understanding of the evolution and selection of antibiotic resistance, and can inform future risk assessment efforts on antibiotic concentrations.

scholarly journals Evaluation of Baffle Fixes Film up Flow Sludge Blanket Filtration (BFUSBF) System in Treatment of Wastewaters from Phenol and 2,4-Dinitrophenol Using Daphnia Magna Bioassay

2016 ◽  
Vol 10 (2) ◽  
pp. 7-11
Author(s):  
Mohammad Javad Ghannadzadeh ◽  
◽  
Ahmad Jonidi Jafari ◽  
Abbas Rezaee ◽  
Fatemeh Eftekharian ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Acute Toxicity ◽  
Daphnia Magna ◽  
Toxicity Test ◽  
Industrial Wastewater ◽  
Acute Toxicity Test ◽  
Toxic Unit ◽  
Appropriate Treatment ◽  
Different Types ◽  
As Toxicity

Background: Phenol and nitrophenol are common compounds found in different types of industrial wastewater known as serious threats to human health and natural environment. In this study, Daphnia magna was used to evaluate the effectiveness of "baffle fixes film up flow sludge blanket filtration" (BFUSBF) system in elimination of phenolic compounds from water. Methods: D. magna cultures were used as toxicity index of phenol and 2,4-DNP mixtures after treatment by a pilot BFUSBF system which consisted of baffle in anoxic section and biofilm in aerobic sections. Initial concentrations were 312 mg/L phenol and 288 mg/L 2,4-dinitrophenol (2,4-DNP). Results: Bioassay tests showed that D. magna was influenced by the toxicity of phenol and 2,4 DNP mixtures. The comparison between the toxicity of initial phenol and 2,4-DNP mixtures and the output toxic unit (TU) derived from BFUSBF treatment system showed that the TU of the effluent from BFUSBF reactor was much lower than that of the solution that entered the reactor. Conclusion: Based on the acute toxicity test, BFUSBF process could reduce phenol and 2,4-DNP in aqueous solutions. Therefore, it is possible to use BFUSBF process as an appropriate treatment option for wastewaters containing phenolic compounds.

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