Overexpressing GmCHI1A increases the isoflavone content of transgenic soybean (Glycine max (L.) Merr.) seeds

AbstractSoybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.

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Variation in Protein and Isoflavone Contents of Collected Domestic and Foreign Soybean (Glycine max (L.) Merrill) Germplasms in Korea

Agriculture ◽

10.3390/agriculture11080735 ◽

2021 ◽

Vol 11 (8) ◽

pp. 735

Author(s):

Ji-Seok Lee ◽

Hong-Sig Kim ◽

Tae-Young Hwang

Keyword(s):

Glycine Max ◽

Protein Content ◽

Negative Correlation ◽

North Korea ◽

Country Of Origin ◽

Average Content ◽

Foreign Countries ◽

Isoflavone Content ◽

The Usa ◽

Glycine Max L

This study was carried out to investigate the variations in protein and isoflavone contents of 300 soybean germplasms introduced from domestic and foreign countries and to compare their contents in terms of size, colour and country of origin. The protein content ranged from 28.7 g 100 g−1 to 44.5 g 100 g−1, with an average of 39.0 g 100 g−1. In a comparison of protein according to country of origin, the highest content was seen in soybeans from Korea (39.7 g 100 g−1), followed by North Korea (39.2 g 100 g−1), China (39.0 g 100 g−1), Japan (38.8 g 100 g−1), the USA (38.0 g 100 g−1) and Russia (37.2 g 100 g−1). The total isoflavone content ranged from 207.0 µg g−1 to 3561.8 µg g−1, with an average of 888.8 µg g−1. In the comparison of isoflavone content according to country, the highest average content was shown in soybeans from Japan (951.3 µg g−1), followed by the USA (918.7 µg g−1), Korea (902.2 µg g−1), North Korea (870.0 µg g−1) and Russia (710.6 µg g−1). Daidzein, glycitein and genistein isoflavone contents were positively correlated, while total isoflavone and protein showed a low negative correlation.

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Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-010-9771-z ◽

2010 ◽

Vol 103 (2) ◽

pp. 227-236 ◽

Cited By ~ 17

Author(s):

Muhammad Zia ◽

Bushra Mirza ◽

Salman Akbar Malik ◽

Muhammad Fayyaz Chaudhary

Keyword(s):

Glycine Max ◽

Flowering Time ◽

Leaf Morphology ◽

Rol Genes ◽

Transgenic Soybean ◽

Plant Phenotype ◽

Glycine Max L

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Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merril] plants at a high frequency

Theoretical and Applied Genetics ◽

10.1007/s001220051441 ◽

2000 ◽

Vol 101 (1-2) ◽

pp. 1-6 ◽

Cited By ~ 98

Author(s):

F. J. L. Aragão ◽

L. Sarokin ◽

G. R. Vianna ◽

E. L. Rech

Keyword(s):

Glycine Max ◽

High Frequency ◽

Transgenic Soybean ◽

Meristematic Cells ◽

Glycine Max L ◽

Selection Of

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Genotypic and environmental variation in seed nutraceutical and industrial composition of non-transgenic soybean (Glycine max) genotypes

Crop and Pasture Science ◽

10.1071/cp14114 ◽

2014 ◽

Vol 65 (12) ◽

pp. 1311 ◽

Cited By ~ 9

Author(s):

Constanza S. Carrera ◽

Julio L. Dardanelli ◽

Diego O. Soldini

Keyword(s):

Glycine Max ◽

Environmental Conditions ◽

Raw Material ◽

Superior Performance ◽

Chemical Components ◽

Transgenic Soybean ◽

Wide Range ◽

Soybean Genotypes ◽

Glycine Max L ◽

Design Production

Genotype × environment interactions (G × E) induce differential response of soybean (Glycine max (L.) Merr.) genotypes to variable environmental conditions with respect to seed composition, and this may hinder breeding progress. The objectives of this study were to estimate the contribution of genotype, environment and G × E to seed chemical composition variability, and to identify the most stable non-transgenic genotypes for several chemical components. Seeds from six non-transgenic soybean genotypes that were grown in 23 environments in Argentina (24–38°S) were analysed. Although environment was the most important source affecting variation for most of the analysed chemical components, genotype and G × E also had a significant effect (P < 0.001). Stable genotypes with superior performance across a wide range of environments were ALIM3.20 for protein, linolenic acid (Len), Len : linoleic acid (LA) ratio (Len/LA), δ-tocopherol (δT) and total isoflavones (TI); ALIM4.13 for protein, oleic acid, α-tocopherol (αT) and δT; ALIM3.14 for Len, αT and TI; Ac0124-1 for Len and Len/LA; and Ac0730-3 for αT. Non-transgenic genotypes with stable chemical profile across environments would perform well under a wide range of environmental conditions for any chemical compound. This study contributes knowledge for breeders to use these genotypes to broaden the genetic backgrounds of currently available commercial cultivars, or to design production strategies that employ the genotypes directly as raw material.

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