Aim. Determination of fatty acid composition of actinobacteria isolated from the surfaces of biological growth of the Odesa gulf of the Black Sea, and their identification. Methods. The 31 isolated strains of actinobacteria were grown in TSB at 28 ° C and 150 rpm for 72 hours. Fatty acid methyl esters of the studied strains were determined according to the MIS Operating Manual on a gas chromatograph Agilent 7890, identification was performed using the identification system of microorganisms MIDI Sherlock. Results. Using chromatographic analysis of fatty acids, it was found that of the 27 studied strains of actinobacteria were identified to the genus Streptomyces, and the 4 strains - to the genus Nocardiopsis. It was found that the fatty acid profiles of the studied actinobacteria of the genus Nocardiopsis were dominated by fatty acids: 15:0 ANTEISO, 16:0 ISO, 17:0 ANTEISO, 18:1 CIS 9, and the fatty acid profiles of bacteria of the genus Streptomyces - 14:0 ISO, 15:0 ANTEISO, 16:0 ISO, 17:0 ANTEISO. Conclusions. Actinobacteria the surfaces of biological growth of the Odesa gulf of the Black Sea belong to the genera Streptomyces and Nocardiopsis, and their fatty acid profiles are characterized by the dominance of isomers of branched saturated fatty acids.