Randomized Study of the Effects of Zinc, Vitamin A, and Magnesium Co-supplementation on Thyroid Function, Oxidative Stress, and hs-CRP in Patients with Hypothyroidism

Abstract Background Antioxidant/oxidant imbalance has been reported to be related to diminished ovarian reserve (DOR). Vitamin A (retinol), a kind of antioxidant, plays a role in restoring ovarian oxidative damage, while C-reactive protein (CRP) is the classical marker of oxidative stress and has recently been identified as an independent variable that is associated with low anti-Mullerian hormone (AMH) levels in young women with DOR. Additionally, retinol binding protein 4 (RBP4) can be considered a substitute for retinol in healthy, nonobese women. The study aim was to determine the relationship between serum RBP4, high sensitivity C-reactive protein (hs-CRP) concentrations and ovarian reserve in nonobese DOR patients. Methods This study included 24 DOR women and 48 normal ovarian reserve (NOR) women from the reproductive medical center of Renmin Hospital of Wuhan University. The serum RBP4 and high-sensitivity CRP (hs-CRP) levels were measured with ELISA kits. Results RBP4 levels (20,648.36 ± 5475.16 ng/ml vs 23,986.48 ± 5995.64 ng/ml, p = 0.025) were decreased, and hs-CRP levels (695.08 ± 1090.19 ng/ml vs 364.32 ± 786.29 ng/ml, p = 0.012) were increased in the DOR group. Serum RBP4 was positively related to AMH (Pearson r = 0.518, p = 0.000), while hs-CRP was negatively correlated with AMH (Spearman r = − 0.345, p = 0.005). after adjustments were made for the covariables, multiple line regression analysis showed that positive association between RBP4 and AMH still existed (β = 0.450, p < 0.001). Conclusion Decreased serum RBP4 levels and increased serum hs-CRP were observed in DOR patients in our study, and the strong correlation between RBP4 and AMH supports the notion that oxidative stress plays a role in DOR, and that appropriate levels of antioxidant vitamin A may be protective against ovarian reserve dysfunction.

Download Full-text

Retinol-binding protein 4 (RBP4) and high sensitivity C-reactive protein (hs-CRP) levels in patients with diminished ovarian reserve (DOR): a cross-sectional study

10.21203/rs.3.rs-73050/v2 ◽

2020 ◽

Author(s):

Wen Zou ◽

Zehao Wang ◽

Jing Xia ◽

Jing Yang

Keyword(s):

Oxidative Stress ◽

Vitamin A ◽

Ovarian Reserve ◽

High Sensitivity ◽

Diminished Ovarian Reserve ◽

Retinol Binding Protein ◽

C Reactive Protein ◽

Retinol Binding Protein 4 ◽

Reactive Protein ◽

Hs Crp

Abstract Background: Antioxidant/oxidant imbalance has been reported to be related to diminished ovarian reserve (DOR). Vitamin A (retinol), a kind of antioxidant, plays a role in restoring ovarian oxidative damage, while C-reactive protein (CRP) is the classical marker of oxidative stress and has recently been identified as an independent variable that is associated with low anti-Mullerian hormone (AMH) levels in young women with DOR. Additionally, retinol binding protein 4 (RBP4) can be considered a substitute for retinol in healthy, nonobese women. The study aim was to determine the relationship between serum RBP4, high sensitivity C-reactive protein (hs-CRP) concentrations and ovarian reserve in nonobese DOR patients.Methods: This study included 24 DOR women and 48 normal ovarian reserve (NOR) women from the reproductive medical center of Renmin Hospital of Wuhan University. The serum RBP4 and high-sensitivity CRP (hs-CRP) levels were measured with ELISA kits.Results: RBP4 levels (20,648.36 ± 5475.16 ng/ml vs 23,986.48 ± 5995.64 ng/ml, p = 0.025) were decreased, and hs-CRP levels (695.08 ± 1090.19 ng/ml vs 364.32 ± 786.29 ng/ml, p = 0.012) were increased in the DOR group. Serum RBP4 was positively related to AMH (Pearson r = 0.518, p = 0.000), while hs-CRP was negatively correlated with AMH (Spearman r = -0.345, p = 0.005). after adjustments were made for the covariables, multiple line regression analysis showed that positive association between RBP4 and AMH still existed (β = 0.450, p ˂ 0.001).Conclusion: Decreased serum RBP4 levels and increased serum hs-CRP were observed in DOR patients in our study, and the strong correlation between RBP4 and AMH supports the notion that oxidative stress plays a role in DOR, and that appropriate levels of antioxidant vitamin A may be protective against ovarian reserve dysfunction.

Download Full-text

Retinol-Binding Protein 4 (RBP4) and High Sensitivity C-Reactive Protein (hs-CRP) Levels in Patients with Diminished Ovarian Reserve (DOR): A Cross-Sectional Study

10.21203/rs.3.rs-73050/v1 ◽

2020 ◽

Author(s):

Wen Zou ◽

Zehao Wang ◽

Jing Xia ◽

Jing Yang

Keyword(s):

Oxidative Stress ◽

Vitamin A ◽

Ovarian Reserve ◽

High Sensitivity ◽

Diminished Ovarian Reserve ◽

Retinol Binding Protein ◽

C Reactive Protein ◽

Retinol Binding Protein 4 ◽

Reactive Protein ◽

Hs Crp

Abstract Background: Antioxidant/oxidant imbalance has been reported to be related to diminished ovarian reserve (DOR). Vitamin A (retinol), a kind of antioxidant, plays a role in restoring ovarian oxidative damage, while C-reactive protein (CRP) is the classical marker of oxidative stress and has recently been identified as an independent variable that is associated with low anti-Mullerian hormone (AMH) levels in young women with DOR. Additionally, retinol binding protein 4 (RBP4) can be considered a substitute for retinol in healthy, nonobese women. The study aim was to determine the relationship between serum RBP4, high sensitivity C-reactive protein (hs-CRP) concentrations and ovarian reserve in nonobese DOR patients.Methods: This study included 24 DOR women and 48 normal ovarian reserve (NOR) women from the reproductive medical center of Renmin Hospital of Wuhan University. The serum RBP4 and high-sensitivity CRP (hs-CRP) levels were measured with ELISA kits.Results: RBP4 levels (20,648.36 ± 5475.16 vs 23,986.48 ± 5995.64, p = 0.025) were decreased, and hs-CRP levels (695.08 ± 1090.19 vs 364.32 ± 786.29, p = 0.012) were increased in the DOR group. Serum RBP4 was positively related to AMH (Pearson r = 0.518, p = 0.000), while hs-CRP was negatively correlated with AMH (Spearman r = -0.345, p = 0.005). after adjustments were made for the covariables, multiple line regression analysis showed that positive association between RBP4 and AMH still existed (β = 0.450, p ˂ 0.001). Conclusion: Decreased serum RBP4 levels and increased serum hs-CRP were observed in DOR patients in our study, and the strong correlation between RBP4 and AMH supports the notion that oxidative stress plays a role in DOR, and that appropriate levels of antioxidant vitamin A may be protective against ovarian reserve dysfunction.

Download Full-text

Effects of Off-Pump Versus On-Pump Coronary Artery Bypass Grafting: Apoptosis, Inflammation, and Oxidative Stress

The Heart Surgery Forum ◽

10.1532/hsf98.2014326 ◽

2014 ◽

Vol 17 (5) ◽

pp. 271 ◽

Cited By ~ 7

Author(s):

Murat Bicer ◽

Tunay Senturk ◽

Murat Yanar ◽

Ahmet Tutuncu ◽

Arzu Yilmaztepe Oral ◽

...

Keyword(s):

Oxidative Stress ◽

Ischemia Reperfusion ◽

Artery Bypass ◽

Bypass Grafting ◽

Artery Bypass Grafting ◽

Cabg Surgery ◽

Off Pump ◽

Pump Cabg ◽

Hs Crp ◽

And Oxidative Stress

Background: It has been suggested that off-pump coronary artery bypass grafting (CABG) surgery reduces myocardial ischemia-reperfusion injury, postoperative systemic inflammatory response, and oxidative stress. The aim of this study was to measure serum malondialdehyde (MDA), highsensitivity C-reactive protein (hs-CRP), M30, and M65 levels and to investigate the relationship between M30 levels and oxidative stress and inflammation in patients undergoing onand off-pump CABG surgery. Methods: Fifty patients were randomly assigned to onpump or off-pump CABG surgery (25 patients off-pump and 25 on-pump CABG surgery), and blood samples were collected prior to surgery, and 30 minutes, 60 minutes, 6 hours, and 24 hours after CABG surgery. Results: Compared to the on-pump group, serum MDA levels at 30 minutes, 60 minutes, 6 hours, and 24 hours after the CABG surgery were significantly lower in the off-pump group (P = .001, P = .001, P = .001, and P = .001, respectively). Serum M30 levels were found to be elevated in both groups, returning to baseline at 24 hours. When compared to baseline, the hs-CRP level reached its peak at 24 hours at 13.28 ± 5.32 mg/dL in the on-pump group, and 15.44 ± 4.02 mg/dL in the off-pump group. Conclusion: CABG surgery is associated with an increase in inflammatory markers and serum M30 levels, indicating epithelial/endothelial apoptosis in the early period.

Download Full-text

Effects of a 10 week Aerobic Exercise on Body Composition, Blood Lipid, Oxidative Stress and hs-CRP of Adult Obese Women

Korean Journal of Sports Science ◽

10.35159/kjss.2017.12.26.6.1007 ◽

2017 ◽

Vol 26 (6) ◽

pp. 1007-1017

Author(s):

Nam-Soo Kim ◽

Seung-Hwan Kim

Keyword(s):

Oxidative Stress ◽

Body Composition ◽

Aerobic Exercise ◽

Blood Lipid ◽

Obese Women ◽

Hs Crp

Download Full-text

Hydrogen Peroxide-Induced Inhibition of Vasomotor Activity: Evaluation of Single and Combined Treatments With Vitamin A and Insulin in Streptozotocin-Diabetic Rats

International Journal of Experimental Diabetes Research ◽

10.1080/15604280214484 ◽

2002 ◽

Vol 3 (2) ◽

pp. 119-130 ◽

Cited By ~ 9

Author(s):

Fulya Zobali ◽

Tanju Besler ◽

Nuray Ari ◽

Çimen Karasu

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Vitamin A ◽

Diabetic Rats ◽

Thiobarbituric Acid Reactive Substance ◽

Single Treatment ◽

Combined Treatments ◽

Retinol Metabolism ◽

Vasomotor Activity ◽

Streptozotocin Diabetic Rats

A positive correlation has been established between increased oxidative stress and cardiovascular diseases in diabetes mellitus. We evaluated the effects of single or combined treatments with vitamin A (retinol acetate, 30 mg/kg/day, for 12-weeks) and insulin (8-10 IU/rat/day for the final 6-week) on vasomotor activity, oxidative stress and retinol metabolism in 12-week streptozotocin diabetic rats. The vasomotor activity was determined by measuring invitroresponsiveness of aorta rings to phenylephrine (PE) and acetylcholine (ACh) in the absence or in the presence of hydrogen peroxide (H2O2). Preincubation withH2O2(10 μM) produced a significant decrease in PE (1 mM)-induced contraction in untreated-diabetic but not in control rats. Single treatment with insulin counteracted this effect ofH2O2and also reversed the increased contractile response of diabetic aorta to PE, while vitamin A was found to be ineffective.H2O2(10 μM) also inhibited ACh (1 mM)-stimulated endothelium- dependent relaxation two fold more in diabetic than in control aorta. In the prevention ofH2O2-induced inhibition of vascular relaxation to ACh, vitamin A alone was markedly effective while insulin alone was not. The combination of vitamin A plus insulin removed the inhibitory action ofH2O2in diabetic aorta. Diabetic animals displayed an increased level of aorta thiobarbituric acid reactive substance (TBARS) in association with decreased levels of plasma retinol and retinol-binding protein (RBP). Single treatment with insulin, in spite of allowing recovery of normal growth rate and improved glucose and retinol metabolism in diabetic rats, was unable to control TBARS production to the same extent as vitamin A alone. Our findings suggest that the maintenance of ACh-stimulated endothelium-dependent vasorelaxant tone in normal physiological levels depends largely on the prevention and/or inhibition of peroxidative stress, which is achieved by combined treatment with vitamin A plus insulin. The use of vitamin A together with insulin provides a better metabolic control and more benefits than use of insulin alone in the reduction of diabetes-induced vascular complications.

Download Full-text

Pathomechanism of Insulin Resistance in Men with Central Obesity: Correlation of GGT, GPx, hs-CRP and Plasma Total Cysteine

The Indonesian Biomedical Journal ◽

10.18585/inabj.v5i2.58 ◽

2013 ◽

Vol 5 (2) ◽

pp. 101 ◽

Cited By ~ 1

Author(s):

Ritawaty Ritawaty ◽

Indriyanti Rafi Sukmawati ◽

Ilhamjaya Patellongi ◽

Ferry Sandra

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Amino Acid ◽

Fatty Liver ◽

Central Obesity ◽

Group Iv ◽

Group Iii ◽

Group I ◽

Group Ii ◽

Hs Crp

BACKGROUND: Gamma glutamyltransferase (GGT) was reported recently to be associated with inflammation, oxidative stress and increased amino acid. However, role of GGT in insulin resistance pathomechanism is not exactly known. Therefore correlation of GGT with inflammation, oxidative stress and elevated amino acid, in men with central obesity need to be confirmed.METHODS: A cross-sectional study was designed. Men with central obesity were recruited and selected. Anthropometric parameters, creatinine, hs-CRP, fasting glucose, fasting insulin, glutathione peroxidase (GPx) activity, GGT, plasma total cysteine (tCys) and fatty liver were measured. Subjects were then divided in 4 groups based on waist circumference (WC) and fatty liver: Group I: WC ≤100 cm, without fatty liver; Group II: WC ≤100 cm, with fatty liver; Group III: WC >100 cm, without fatty liver; Group IV: WC >100 cm, with fatty liver. All biochemical characteristics in each group were then statistically analyzed.RESULTS: Seventy-two men with central obesity were selected. Numbers of subjects in each group were: Group I: n=33; Group II: n=5; Group III: n=17; Group IV: n=17. We found significant difference of HOMA-IR between Group I and IV, significant correlation between GGT and HOMAIR, and significant negative correlation between tCys with HOMA-IR in Group IV.CONCLUSION: GGT was significantly correlated with HOMA-IR in men with WC >100 cm and fatty liver. Further investigation with more subjects is necessary to determine clear GGT cut-off to distinguish subjects with fatty liver and insulin resistance.KEYWORDS: GGT, hs-CRP, GPx, tCys, HOMA-IR, insulin resistance

Download Full-text

Relationship Between gamma-Glutamyltransferase (gamma-GT) with High Sensitive C-Reactive Protein (hs-CRP), Oxidized (Ox-LDL) and Glutathione Peroxidase (GPx) on Coronary Heart Disease (CHD) Patient

The Indonesian Biomedical Journal ◽

10.18585/inabj.v1i2.94 ◽

2009 ◽

Vol 1 (2) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Marissa Arifin ◽

Marsetio Donosepoetro ◽

Sutomo Kasiman

Keyword(s):

Oxidative Stress ◽

Coronary Heart Disease ◽

Heart Disease ◽

Glutathione Peroxidase ◽

Medical Center ◽

Oxidized Ldl ◽

C Reactive Protein ◽

Reactive Protein ◽

Significant Difference ◽

Hs Crp

BACKGROUND: Recent clinical studies have suggested that γ-glutamyltransferase (γ-GT) can trigger oxidative stress within the plaque. This study aimed to investigate whether serum γ-GT might be as a risk factor of coronary heart disease (CHD), and measure the associations of serum γ-GT with high sensitive C-Reactive Protein (hs-CRP), Oxidized LDL (Ox-LDL) and Glutathione Peroxidase (GPx).METHODS: This study recruited 48 patients aged 30-70 year who underwent coronary angiography at Haji Adam Malik Medical Center at Medan between February and April 2008 and who presented at least one coronary stenosis of > 50% of the luminar diameter. The sample subjects were consecutively selected.RESULTS: γ-Glutamyltransferase was positively associated (r = 0.546) with hs-CRP as a marker of chronic inflammation after careful adjustment for other established risk factors in CHD patient. But, there was no significant difference between γ-GT in male and female patients. Further, there were no correlations between γ-GT and Ox-LDL and GPx. Ratio of γ-GT/GPx was measured as well, and it was associated with hs-CRP.CONCLUSIONS: Ratio of γ-GT/GPx was associated with inflammation process in coronary heart disease patients.KEYWORDS: γ-glutamyltransferase (γ-GT), inflammation, oxidative stress, coronary heart disease

Download Full-text