Biocompatibility of Poly[di(carboxylatophenoxy)-phosphazene] Polymer: In Vitro Cytotoxicity in Cell Culture

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

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Prediction of tumor response of rectal cancer cells via 3D cell culture and in�vitro cytotoxicity assay before initiating preoperative chemoradiotherapy

Oncology Letters ◽

10.3892/ol.2019.10702 ◽

2019 ◽

Author(s):

Jeonghyun Kang ◽

Min Park ◽

Jina Kim ◽

Hyuk Hur ◽

Byung Min ◽

...

Keyword(s):

Rectal Cancer ◽

Cell Culture ◽

Cancer Cells ◽

Tumor Response ◽

3D Cell Culture ◽

Preoperative Chemoradiotherapy ◽

In Vitro Cytotoxicity ◽

Cytotoxicity Assay

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In vitro cytotoxicity of Dragon Fruit Extract (Hylocereus polyrhizus) on Fibroblast Cell Culture

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.04.195 ◽

2020 ◽

Vol 12 (04) ◽

Keyword(s):

Cell Culture ◽

Fibroblast Cell ◽

In Vitro Cytotoxicity ◽

Fruit Extract ◽

Dragon Fruit ◽

Fibroblast Cell Culture ◽

Hylocereus Polyrhizus

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Loss of in vitro cytotoxicity of cisplatin after storage as stock solution in cell culture medium at various temperatures

Cancer ◽

10.1002/(sici)1097-0142(19970501)79:9<1723::aid-cncr13>3.0.co;2-# ◽

1997 ◽

Vol 79 (9) ◽

pp. 1723-1728 ◽

Cited By ~ 11

Author(s):

Horst Schuldes ◽

Sven Bade ◽

Jutta Knobloch ◽

Dietger Jonas

Keyword(s):

Cell Culture ◽

Culture Medium ◽

In Vitro Cytotoxicity ◽

Cell Culture Medium

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Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

Polymers ◽

10.3390/polym12030590 ◽

2020 ◽

Vol 12 (3) ◽

pp. 590 ◽

Cited By ~ 1

Author(s):

Andrea Catalina Villamil Ballesteros ◽

Hugo Ramiro Segura Puello ◽

Jorge Andres Lopez-Garcia ◽

Andres Bernal-Ballen ◽

Diana Lorena Nieto Mosquera ◽

...

Keyword(s):

Cell Culture ◽

Extracellular Matrix ◽

In Vitro Cytotoxicity ◽

Epithelial Tissue ◽

Scanning Calorimetry ◽

Skin Cell ◽

New Material ◽

Ecm Architecture ◽

Amniotic Membranes

Decellularized membranes (DM) were obtained from bovine amniotic membranes (BAM) using four different decellularization protocols, based on physical, chemical, and mechanical treatment. The new material was used as a biological scaffold for in vitro skin cell culture. The DM were characterized using hematoxylin-eosin assay, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR-ATR), and differential scanning calorimetry (DSC). The in vitro cytotoxicity of DM was evaluated using MTT. The efficacy of decellularization process was assessed through DNA quantification and electrophoresis. All the used protocols showed a high effectiveness in terms of elimination of native cells, confirmed by DNA extraction and quantification, electrophoresis, and SEM, although protocol IV removes the cellular contents and preserve the native extracellular matrix (ECM) architecture which it can be considered as the most effective in terms of decellularization. FTIR-ATR and DSC on the other hand, revealed the effects of decellularization on the biochemical composition of the matrices. There was no cytotoxicity and the biological matrices obtained were a source of collagen for recellularization. The matrices of protocols I, II, and III were degraded at day 21 of cell culture, forming a gel. The biocompatibility in vitro was demonstrated; hence these matrices may be deemed as potential scaffold for epithelial tissue regeneration.

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Novel phytochemical Cissus quadrangularis extract–loaded chitosan/Na-carboxymethyl cellulose–based scaffolds for bone regeneration

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911518793913 ◽

2018 ◽

Vol 33 (6) ◽

pp. 629-646 ◽

Cited By ~ 5

Author(s):

Sedef Tamburaci ◽

Ceren Kimna ◽

Funda Tihminlioglu

Keyword(s):

Cell Culture ◽

Bone Regeneration ◽

Bone Tissue ◽

Carboxymethyl Cellulose ◽

Average Pore Size ◽

In Vitro Cytotoxicity ◽

Bone Tissue Regeneration ◽

Average Pore ◽

Cissus Quadrangularis

Medicinal plants are attracting considerable interest as a potential therapeutic agent for bone tissue regeneration. Cissus quadrangularis L. is also a medicinal plant known with its osteogenic activity. In this study, a phytochemical scaffold was produced by incorporating Cissus quadrangularis with chitosan/Na-carboxymethyl cellulose blend by lyophilization technique. The effect of Cissus quadrangularis loading on the mechanical, morphological, chemical, and degradation properties as well as in vitro cytotoxicity, cell proliferation, and differentiation of the composites was investigated. Scanning electron microscopy images showed that porous Cissus quadrangularis–loaded scaffolds were obtained with an average pore size of 148–209 µm which is appropriate for bone regeneration. Cissus quadrangularis incorporation enhanced the compression modulus of scaffolds from 76 to 654 kPa. In vitro cell culture results indicated that Cissus quadrangularis/chitosan/Na-carboxymethyl cellulose scaffolds provided a favorable substrate for the osteoblast adhesion, proliferation, and mineralization. Results supported the osteoinductive property of the Cissus quadrangularis extract–incorporated scaffolds even without osteogenic media supplement. Cissus quadrangularis extract increased the alkaline phosphatase activity of the SaOS-2 cells on scaffolds on 7th and 14th days of incubation. The investigation of characterization and cell culture studies suggest that Cissus quadrangularis–loaded osteoinductive Cissus quadrangularis/chitosan/Na-carboxymethyl cellulose scaffold can serve as a potential biomaterial for bone tissue engineering applications.

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Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Revista de Chimie ◽

10.37358/rc.19.4.7084 ◽

2019 ◽

Vol 70 (4) ◽

pp. 1157-1161 ◽

Cited By ~ 1

Author(s):

Rodica Tatia ◽

Christina Zalaru ◽

Isabela Tarcomnicu ◽

Lucia Moldovan ◽

Oana Craciunescu ◽

...

Keyword(s):

Cell Culture ◽

Antitumor Activity ◽

Ethanolic Extract ◽

L929 Cell ◽

In Vitro Cytotoxicity ◽

In Vitro Tests ◽

Cervix Carcinoma ◽

Hedera Helix ◽

Isolation And Characterization

Hederagenin, a saponin known for its therapeutic effect was isolated from leaves of Hedera helix. Hederagenin was obtained by repeated maceration of ground plant material in 95% (v/v) ethanol. The ethanolic extract was subjected to acid hydrolysis and purification with acetonitrile. Isolated hederagenin (IHe) was analyzed by HPLC-MS/MS and compared to standard hederagenin. In vitro cytotoxicity of IHe was tested in a culture of fibroblast cells from NCTC clone L929 cell line by MTT assay, using dioscin as positive control. For the determination of IHe antitumor effect, in vitro tests were performed in a culture of human cervix carcinoma Hep-2 cells cultivated in the presence of different concentrations of sample for 48 h. IHe was biocompatible in the range of concentrations 2-200 mg/mL, in NCTC cell culture. The compound showed cell cytotoxicity in the concentration range of 100-400 mg/mL, in Hep-2 cell culture, revealing its antitumor activity. These results demonstrated the possible use of hederagenin isolated from H. helix extract as an antitumor agent.

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