scholarly journals Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

2016 ◽  
Vol 22 (3) ◽  
pp. 321-330 ◽  
Author(s):  
Massoume Amini ◽  
Ali Deljou ◽  
Haidar Saify Nabiabad
Keyword(s):  
Medicago Sativa ◽  
Somatic Embryos ◽  
Transformation Efficiency ◽  
Plantlet Regeneration ◽  
Embryo Maturation ◽  
Cuscuta Campestris ◽  
Medicago Sativa L

A transplant plug technique for production of alfalfa (Medicago sativa L.) plants from somatic embryos

1992 ◽  
Vol 72 (2) ◽  
pp. 483-485 ◽  
Author(s):  
A. R. McElroy ◽  
D. C. W. Brown
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Somatic Embryos ◽  
Potting Media ◽  
Medicago Sativa L

A transplant plug technique was developed that uses in vitro somatic embryogenesis techniques to mass-multiply alfalfa plants in a form suitable for direct transplanting. The plug contains potting media covered with an agar nutrient cap. Plants develop from embryos placed on the cap and then establish in the potting media.Key words: Hybrid alfalfa, Medicago sativa L., tissue culture, transplant plug, somatic embryogenesis

scholarly journals EMBRIOGENESIS SOMATIK IN VITRO DAN REGENERASI PLANLET DARI TIGA VARIETAS ALFALFA (Medicago sativa L.)

2019 ◽  
Vol 6 (1) ◽  
pp. 83
Author(s):  
Sulastri Sulastri ◽  
Winda Nawfetrias ◽  
Djatmiko Pinardi ◽  
Henti Rosdayanti
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Sativa ◽  
Callus Induction ◽  
Plantlet Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Medicago Sativa L ◽  
Embryogenic Callus Induction ◽  
2,4 Dichlorophenoxyacetic Acid

In Vitro Somatic Embryogenesis and Plantlet Regeneration of Three Varieties of Alfalfa (Medicago sativa L.)ABSTRACTAlfalfa (Medicago sativa L.) is a valuable plant as a source of food for animal, forage, pharmaceutical, medicine, food supplement, and human consumption.  In vitro selection technology combined with induction or spontaneous mutagenesis has been effective in altering or isolating genetic variability for desirable characters.  Consequently, a reproducible in vitro propagation technique of that plant is mandatory. The aim of the research was to obtain information on the embryogenic callus induction, somatic embryogenesis, and plantlet regeneration of three varieties of alfalfa. The results showed that an optimum embryogenic callus induction (82%) was obtained on Murashige & Skoog (MS) basal medium containing 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin and 2 ppm a-naphthaleneacetic acid (NAA). Those embryogenic calli could subsequently develop into somatic embryos, which germinated and regenerated into normal plantlets on R1 medium consisting of MS nutrients without the addition of plant growth regulator.Keywords: alfalfa, callus, embryogenic, plantlets, regeneration ABSTRAKAlfalfa (Medicago sativa) adalah tanaman berharga sebagai sumber makanan untuk hewan, yaitu hijauan pakan ternak, farmasi, obat-obatan, suplemen makanan dan konsumsi manusia. Teknologi seleksi in vitro yang dikombinasikan dengan induksi atau mutagenesis spontan telah terbukti efektif dalam mengubah atau mengisolasi variabilitas genetik untuk karakter yang diinginkan. Oleh sebab itu, keberhasilan teknik perbanyakan in vitro yang telah terbukti dapat direproduksi dari tanaman tersebut menjadi syarat yang harus terpenuhi. Tujuan dari penelitian ini adalah untuk mendapatkan informasi mengenai induksi kalus embriogenik, embriogenesis somatik dan regenerasi planlet dari tiga varietas alfalfa. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik optimal (82%) didapat pada media Murashige & Skoog (MS) dengan  2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin dan 2 ppm a-naphthaleneacetic acid (NAA). Kalus embriogenik tersebut dapat membentuk embrio somatik, embrionya berkecambah dan beregenerasi membentuk planlet normal pada perlakuan media R1 yaitu nutrisi MS tanpa penambahan zat pengatur tumbuh.Kata Kunci: alfalfa, embriogenik, kalus, planlet, regenerasi

Insecticidal Active Rotenoids from Plant Parts and Callus Culture of Medicago sativa L. from a Semiarid Region of India (Rajasthan)

2020 ◽  
Vol 16 (6) ◽  
pp. 937-941
Author(s):  
Sharad Vats ◽  
Preeti Mehra
Keyword(s):  
Medicago Sativa ◽  
Callus Culture ◽  
Point Of View ◽  
Semiarid Region ◽  
Disease Vectors ◽  
Vector Borne Diseases ◽  
Plant Parts ◽  
Resistant Varieties ◽  
Medicago Sativa L

Background: Vector-borne diseases are quite prevalent globally and are one of the major causes of deaths due to infectious diseases. There is an availability of synthetic insecticides, however, their excessive and indiscriminate use have resulted in the emergence of resistant varieties of insects. Thus, a search for novel biopesticide has become inevitable. Methods: Rotenoids were isolated and identified from different parts of Medicago sativa L. This group of metabolites was also identified in the callus culture, and the rotenoid content was monitored during subculturing for a period of 10 months. Enhancement of the rotenoid content was evaluated by feeding precursors in a tissue culture medium. Results: Four rotenoids (elliptone, deguelin, rotenone and Dehydrorotenone) were identified, which were confirmed using spectral and chromatographic techniques. The maximum rotenoid content was found in the seeds (0.33±0.01%), followed by roots (0.31±0.01%) and minimum in the aerial parts (0.20±0.05%). A gradual decrease in the rotenoid content was observed with the ageing of subcultured tissue maintained for 10 months. The production of rotenoids was enhanced up to 2 folds in the callus culture using amino acids, Phenylalanine and Methionine as precursors as compared to the control. The LC50 value of the rotenoids was found to be 91 ppm and 162 ppm against disease vectors of malaria and Dracunculiasis, respectively. Conclusion: The study projects M. sativa as a novel source of biopesticide against the disease vectors of malaria and Dracunculiasis. The use of precursors to enhance the rotenoid content in vitro can be an effective venture from a commercial point of view.

Enhanced vigor of dry somatic embryos of Medicago sativa L. with increased sucrose

Plant Science ◽  
1990 ◽  
Vol 71 (2) ◽  
pp. 261-266 ◽  
Author(s):  
Kandiah Anandarajah ◽  
Bryan D. McKersie
Keyword(s):  
Medicago Sativa ◽  
Somatic Embryos ◽  
Medicago Sativa L

EFFECTS OF EARLY SUMMER AND FALL CUTTING MANAGEMENT ON DRY MATTER YIELDS, DIGESTIBILITY, CRUDE PROTEIN, AND SURVIVAL OF SARANAC AND NARRAGANSETT ALFALFAS

1972 ◽  
Vol 52 (6) ◽  
pp. 941-948 ◽  
Author(s):  
L. B. MacLEOD ◽  
H. T. KUNELIUS ◽  
F. W. CALDER
Keyword(s):  
Medicago Sativa ◽  
Crude Protein ◽  
Dry Matter ◽  
Early Summer ◽  
Good Survival ◽  
Considerable Decrease ◽  
Dry Matter Digestibility ◽  
Medicago Sativa L ◽  
Cutting Management

The effects of early summer and fall cutting managements on two alfalfa (Medicago sativa L.) cultivars were studied over a 3-year period. Removal of the first crop of Saranac and Narrangansett alfalfas at prebud stage, followed by two cuts at the early bloom stage before early September, and a late fall harvest on October 25, produced seasonal dry matter (DM) yields of 7.5–8.6 metric T/ha in the 1st year. Stand deterioration, however, was severe and by the 3rd year, practically all alfalfa had disappeared. Average percent crude protein (CP) in cut 1 was 17.7–21.9 and percent in vitro dry matter digestibility (IVD) 67.4–73.8. Removal of the first cut at bud stage with two subsequent harvests at the early bloom stage also allowed a total of three harvests before early September and resulted in seasonal DM yields of 8.6–8.8 in the 1st year. Although the survival of alfalfa was increased over the managements where cut 1 was at prebud stage, a considerable decrease of alfalfa was still observed in the 3rd year. Under this management, average CP in cut 1 was 15.8–16.3 and IVD 64.1–68.4. Harvesting the first crop at early to midbloom stage and the second crop at early bloom in early to mid-August, followed by third harvest in late October, resulted in good survival with seasonal DM yields of 6.1–10.6. Lower CP and IVD were recorded in cut 1 than in the more intensive managements. Under all managements, average CP in cuts 2 and 3 was 14.9–17.3 and 13.3–19.4, respectively. IVD was 54.5–60.7 in cut 2 and 56.0–64.4 in cut 3.

GENETIC VARIATION FOR FORAGE QUALITY OF ALFALFA STEMS

1987 ◽  
Vol 67 (4) ◽  
pp. 1057-1067 ◽  
Author(s):  
D. R. BUXTON ◽  
G. C. MARTEN ◽  
J. S. HORNSTEIN
Keyword(s):  
Cell Wall ◽  
Genetic Variation ◽  
Medicago Sativa ◽  
Crude Protein ◽  
Nutritive Value ◽  
Growth Traits ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Medicago Sativa L

When normally harvested for hay, the basal portion of alfalfa (Medicago sativa L.) stems is low in forage quality. This study was conducted to determine whether genetic variation for in vitro digestible dry matter (IVDDM) and crude protein (CP) concentration of stems of perennial Medicago spp. could be identified and whether morphological and chemical traits were associated with variation in these characteristics of stems. The lower six nodes and internodes of stems and total herbage from 64 Plant Introduction (PI) accessions and five cultivars of field-grown plants were harvested twice when most plants were at the early seed-pod stage of maturity. Several morphological and growth traits were measured, and both stems and total herbage were evaluated for IVDDM and CP concentration. Covariant analysis was used to adjust in IVDDM and CP concentration of stems for differences in plant maturity. Significant variation for IVDDM and CP concentration in stems existed among the cultivars and among the accessions. Some accessions had significantly higher stem IVDDM or CP concentration than control cultivars. The M. sativa accession, PI 440496, had the fourth highest stem IVDDM and third highest stem CP, with concentrations of 374 and 79 g kg−1, respectively. The number of ridges on stems (stem shape) was significantly correlated with stem IVDDM and CP concentration with average correlation coefficients of 0.30 and 0.35, respectively. Cell-wall concentration and lignin concentration per unit cell wall of stems were both negatively correlated with stem IVDDM and CP concentration. Selection for improved nutritive value of stems may have potential for improving the nutritive value of alfalfa herbage.Key words: Alfalfa, forage quality, crude protein, in vitro digestibility, Medicago sativa L.

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