Adverse and beneficial effects of long-term high-concentration ascorbic acid supplementation in rainbow trout Oncorhynchus mykiss

2011 ◽  
Vol 77 (6) ◽  
pp. 1009-1014 ◽  
Author(s):  
Takanori Ishikawa ◽  
Nobuhiro Mano ◽  
Teruyuki Nakanishi ◽  
Hitomi Hirose
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
High Concentration ◽  
Acid Supplementation ◽  
Beneficial Effects ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Ascorbic Acid Supplementation

scholarly journals Protective Effect of Vitamin C (Ascorbic Acid) Supplementation on Post-Thaw Motility and Fertility of Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Sperm

2020 ◽  
Vol 8 (6) ◽  
pp. 1348-1352
Author(s):  
Uğur Yavuz ◽  
Yusuf Bozkurt
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Liquid Nitrogen ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dmso Concentration ◽  
Ascorbic Acid Supplementation

Ascorbic acid (Vitamin C) is one of the important antioxidants, which naturally present in seminal plasma of fish. On the other hand, whether its effect may improve sperm quality following cryopreservation process still remains its uncertainty. Thus, the present study aimed to analyse the effect of different extenders supplemented with different ascorbic acid concentrations on post-thaw motility and fertility of frozen-thawed rainbow trout (Oncorhynchus mykiss) sperm. Selected sperm samples were pooled and diluted at 1:3 ratios with two different extenders (E) composing such as (E-1) 300 mM glucose, 10% egg yolk and 10% DMSO and (E-2) 0.6 mM sucrose and 10% DMSO. Each extender was supplemented with vitamin C at 1, 5 and 10 mM concentrations. Following dilution, the sperm was loaded into 0.25 ml straws and frozen in liquid nitrogen vapour. The straws were then plunged into liquid nitrogen for storage. Fertilization was carried out using the dry fertilization technique. Highest post-thaw motility (50±5.77) and fertilization (56±1.00) results were obtained with the extender-1 (E-1) containing 10% DMSO concentration. In conclusion, the present study indicated that addition of ascorbic acid to the extenders improved rainbow trout sperm motility resulting higher fertilization of the eggs.

scholarly journals Long-term exposure to low 17α-ethinylestradiol (EE2) concentrations disrupts both the reproductive and the immune system of juvenile rainbow trout, Oncorhynchus mykiss

2020 ◽  
Vol 142 ◽  
pp. 105836
Author(s):  
Kristina Rehberger ◽  
Elena Wernicke von Siebenthal ◽  
Christyn Bailey ◽  
Patrick Bregy ◽  
Melanie Fasel ◽  
...  
Keyword(s):  
Immune System ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Juvenile Rainbow Trout ◽  
Rainbow Trout Oncorhynchus Mykiss

Effect of Dietary Ascorbate on the Concentration of Tissue Ascorbic Acid, Dehydroascorbic Acid, Ascorbic Sulfate, and Activity of Ascorbic Sulfate Sulfohydrolase in Rainbow Trout (Oncorhynchus mykiss)

1990 ◽  
Vol 47 (8) ◽  
pp. 1518-1525 ◽  
Author(s):  
Konrad Dabrowski ◽  
Reinhard Lackner ◽  
Cristine Doblander
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Dehydroascorbic Acid ◽  
Control Group ◽  
High Demand ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dietary Treatments ◽  
Kidney Liver

The concentrations of ascorbic acid in several tissues of rainbow trout (Oncorhynchus mykiss) are significantly influenced by various dietary treatments. Ascorbic acid was taken up readily by erythrocytes, kidney, liver, intestine, spleen, and brain in fish fed an ascorbate supplemented diet (AA group), the concentration being from 1.5 to 14.8-fold higher than in fish fed a diet lacking ascorbate (control group). In fish fed a diet supplemented with an equimolar amount of ascorbic acid in the form of ascorbic sulfate (AS group) the ascorbic acid concentrations in kidney, intestine, and erythrocytes were significantly elevated above those of the control group. Ascorbic sulfate was found in kidney, liver, and intestine of the AS group, but not in other groups. In fish fed a diet devoid of vitamin C the ascorbic acid concentrations in kidney, liver, intestine, and spleen were signficantly lower than in fasting fish over the same period of time (28 d), suggesting a high demand for vitamin C in an actively feeding animal. Salmonid fish are therefore probably unable to utilize ascorbic sulfate sufficiently to prevent the appearance of vitamin C deficiency, and thus resemble scurvy-prone mammals in this respect.

Interactions among Antioxidant Defenses in Liver of Rainbow Trout (Oncorhynchus mykiss) Exposed to Cadmium

1993 ◽  
Vol 50 (1) ◽  
pp. 156-162 ◽  
Author(s):  
V. P. Palace ◽  
H. S. Majewski ◽  
J. F. Klaverkamp
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Blood Cells ◽  
Physiological Saline ◽  
Antioxidant Defenses ◽  
Glutathione Peroxidase Activity ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cd Exposure ◽  
Erythrocyte Fragility

Rainbow trout (Oncorhynchus mykiss) (mean weight 180 ± 10 g) fed diets deficient in ascorbic acid, tocopherol, both, or neither were exposed to one of three cadmium concentrations (0, 2, or 4 μg Cd/L). After 181 d of exposure, liver and whole blood were sampled. Cadmium exposure was found to significantly increase the Cd content in liver, especially in fish fed a diet deficient in ascorbic acid. Hepatic stores of ascorbic acid decreased in fish exposed to Cd and in fish fed diets deficient in tocopherol and ascorbic acid. Depletion of tocopherol in liver occurred only in those fish fed a diet deficient in tocopherol and exposed to Cd. Superoxide dismutase activity in liver increased with Cd exposure and with dietary deficiencies of tocopherol and ascorbic acid, while catalase was inhibited by exposure to Cd alone. Glutathione peroxidase activity was unaffected by Cd but was lower in fish fed a diet deficient in both tocopherol and ascorbic acid. Erythrocyte fragility, measured by spontaneous hemolysis of red blood cells in physiological saline, was increased by Cd exposure and the deficiencies of dietary tocopherol and ascorbic acid.

Long-term primary culture of epithelial cells from rainbow trout (Oncorhynchus mykiss) liver

1995 ◽  
Vol 31 (5) ◽  
pp. 367-378 ◽  
Author(s):  
Gary K. Ostrander ◽  
James B. Blair ◽  
Beverly A. Stark ◽  
Garry M. Marley ◽  
Wesley D. Bales ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Epithelial Cells ◽  
Oncorhynchus Mykiss ◽  
Primary Culture ◽  
Rainbow Trout Oncorhynchus Mykiss

Competition for space and food in rainbow trout, Oncorhynchus mykiss, as related to ascorbic acid status

Aquaculture ◽  
1999 ◽  
Vol 180 (1-2) ◽  
pp. 79-87 ◽  
Author(s):  
J.H Blom ◽  
K Dabrowski ◽  
J.D Rapp ◽  
Y Sakakura ◽  
K Tsukamoto
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Competition For Space ◽  
Acid Status

scholarly journals Effects of dietary ascorbic acid levels on cholesterol metabolism in rainbow trout (Oncorhynchus mykiss)

2019 ◽  
Vol 25 (6) ◽  
pp. 1345-1353
Author(s):  
Jun‐Ming Deng ◽  
Xin‐Dang Zhang ◽  
Jian‐Wei Zhang ◽  
Bao‐Liang Bi ◽  
Heng‐Zhi Wang ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Cholesterol Metabolism ◽  
Rainbow Trout Oncorhynchus Mykiss

Absorption of Ascorbic Acid and Ascorbic Sulfate and their Interaction with Minerals in the Digestive Tract of Rainbow Trout (Oncorhynchus mykiss)

1989 ◽  
Vol 46 (11) ◽  
pp. 1952-1957 ◽  
Author(s):  
K. Dabrowski ◽  
G. Köck
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Concentration Factor ◽  
Food Level ◽  
Mineral Contents ◽  
Posterior Intestine ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Intestinal Contents ◽  
Concentration Factors

The sites of absorption of ascorbic acid (AA) in the rainbow trout (Oncorhynchus mykiss) gut were located in the stomach (20.7%), pyloric caecae region (23.4%), middle intestine (21.9%) and posterior intestine (20.1%). Ascorbic sulfate (AS) were found to be absorbed in the stomach (27.7%) although it is likely that the figure obtained is an overestimate. This conclusion is based on the concentration factor of the intestinal contents (faeces/food ratio) being much higher for ascorbic sulfate than for an external dietary marker, chromic oxide. The apparent protein absorption rates from the three diets used were not significantly different (P < 0.01), ranging from 86.9 to 91.5%. Three subsequent samplings, one week apart, did not reveal changes in AA or AS absorption in trout, suggesting that fish were neither "saturated" with AA nor had they developed the means to utilize the dietary AS. In most cases, the mineral contents in faeces were similar for all diets, although the concentration factors (faeces/food level), varied greatly during the course of the trial (1.9–7.1 for Zn, 1.8–4.9 for Fe, and 2.4–3.7 for Cu). The concentrations of Fe and Zn in the contents of the posterior intestine were lower in the fish fed an AA-supplemented diet than in fish fed on AS and deficient diets, which may suggest an increased absorption of these elements.

Branchial Morphological and Endocrine Responses of Rainbow Trout (Oncorhynchus mykiss) to a Long-Term Sublethal Acid Exposure In Which Acclimation Did Not Occur

1993 ◽  
Vol 50 (1) ◽  
pp. 198-209 ◽  
Author(s):  
Céline Audet ◽  
Chris M. Wood
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Chloride Cell ◽  
Chloride Cells ◽  
Physiological Data ◽  
Mucous Cells ◽  
Diffusion Distance ◽  
Cell Numbers ◽  
Rainbow Trout Oncorhynchus Mykiss

Changes in branchial morphology and in plasma Cortisol, adrenaline, and noradrenaline were quantified throughout an 81 -d exposure of rainbow trout (Oncorhynchus mykiss) to sublethal acidity (pH 4.8) in artificial soft water and after a 5-h acid challenge (pH 4.0) of naive fish and 81-d acid-preexposed fish. Changes in branchial morphology at pH 4.8 were generally very mild and characterized by slight increases in filamental mucous cells and decreases in lamellar mucous cells. Chloride cell numbers and branchial Na+–K+- and total ATPase activities did not change. The filamental epithelium thickened, but the water–blood diffusion distance in the lamellae decreased during chronic exposure. Cortisol was significantly elevated throughout whereas catecholamines exhibited relatively little response. Response to acute pH 4.0 challenge was similar in naive and 81-d acid-exposed fish: epithelial damage, increase in visible mucous cells, loss of chloride cells by necrosis, and high cortisol levels but no changes in lamellar or filamental epithelial thickness, diffusion distance, ATPase activities, or catecholamine levels. Previously reported physiological data from these same trout demonstrated that sensitization rather than acclimation had occurred. Therefore, these observations support the view that acclimation does not occur in the absence of significant branchial damage and repair.

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