scholarly journals Beneficial effects of thyme (Thymus vulgaris ) extract on oxytetracycline-induced stress response, immunosuppression, oxidative stress and enzymatic changes in rainbow trout (Oncorhynchus mykiss )

2018 ◽  
Vol 25 (2) ◽  
pp. 298-309 ◽  
Author(s):  
Seyyed Morteza Hoseini ◽  
Morteza Yousefi
Keyword(s):  
Oxidative Stress ◽  
Rainbow Trout ◽  
Stress Response ◽  
Oncorhynchus Mykiss ◽  
Thymus Vulgaris ◽  
Beneficial Effects ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Induced Stress ◽  
Enzymatic Changes

scholarly journals The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2)

2021 ◽  
Vol 38 (3) ◽  
pp. 269-273
Author(s):  
Mehmet Reşit Taysı ◽  
Muammer Kırıcı ◽  
Mahinur Kırıcı ◽  
Hasan Ulusal ◽  
Bünyamin Söğüt ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Liver Tissue ◽  
Stress Index ◽  
Mercury Chloride ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Total Antioxidant ◽  
Ld50 Value

The aim of this study was to determine oxidative stress caused by mercury chloride (HgCl2) in rainbow trout (Oncorhynchus mykiss) liver tissue. For this purpose, the LD50 value of HgCl2 on rainbow trout was determined as 551 μg/L. In the study, 40 fish in four groups were exposed to 25% and 50% (138 and 276 µg/L) of the two subletal doses of HgCl2 for 2 and 7 days, with 10 fish (n=10) in each group. To determine oxidative stress; peroxynitrite (ONOO−), total oxidant level (TOS), total antioxidant level (TAS), oxidative stress index (OSI) and malondialdehyde (MDA) were analyzed. In the study, it was observed that the differences between the groups in terms of ONOO−, TOS, TAS and OSI levels in the liver tissues was significant (P<0.05), however, this difference was not significant (P>0.05) in terms of MDA values. As a result, it can be concluded that HgCl2 increases ONOO−, TOS, TAS, OSI and MDA levels in liver tissue and even small doses of mercury are toxic to fish.

scholarly journals POST-VACCINATED ALTERATIONS IN THE MARKERS OF LIPID AND PROTEIN OXIDATION IN THE GILLS OF RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM) IMMUNIZED AGAINST THE ENTERIC REDMOUTH DISEAS

2020 ◽  
pp. 24-35
Author(s):  
Halyna Tkachenko ◽  
Natalia Kurhaluk ◽  
Joanna Grudniewska ◽  
Agnieszka Pękala-Safińska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Oxidative Stress Biomarkers ◽  
Enzymatic Assays ◽  
Stress Biomarkers ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Derivatives Of ◽  
Isolation Buffer

The aim of the study was the evaluation of the content of oxidative stress biomarkers (2-thiobarbituric-acid-reacting substances as a biomarker of lipid peroxidation, aldehydic and ketonic derivatives of oxidatively modified proteins) in the gills of rainbow trout (Oncorhynchus mykissWalbaum) vaccinated by a vaccine against Yersiniaruckeri. Rainbow trout (Oncorhynchus mykiss Walbaum) with a mean body mass of (107.9±3.1) g were used in the experiments. The study was carried out in a Department of Salmonid Research, Inland Fisheries Institute in Rutki (Poland). Experiments were performed at a water temperature of 14.5±0.5°C and the pH was 7.5. The dissolved oxygen level was about 12 ppm with additional oxygen supply with a water flow of 25 L per min, a photoperiod of 12 hours per day. The fish were fed with a commercial pelleted diet at an optimal level, using 12-hour belt feeders for fish. All enzymatic assays were carried out at the Department of Zoology and Animal Physiology, Institute of Biology and Earth Sciences, Pomeranian University in Słupsk (Poland).The fish were kept for 60 days after vaccination at a water temperature of 14.5±0.5°C and pH 7.5. In our study, 15 rainbow trout from unhandled control and 15 vaccinated trout were used. Two months after immunization, samples from rainbow trout were collected. The fish were captured and killed 61 days post-vaccination (n = 15 in each group). Gills were removed in situ. The organs were rinsed clear of blood with cold isolation buffer and homogenized using a glass homogenizer H500 with a motor-driven pestle immersed in an ice water bath to yield a homogenate in proportion 1:9 (weight/volume). The isolation buffer contained 100 mMTris-HCl; a pH of 7.2 was adjusted with HCl. Homogenates were centrifuged at 3,000g for 15 min at 4°C. After centrifugation, the supernatant was collected and frozen at −20°C until analyzed. Protein contents were determined using the method of Bradford (1976) with bovine serum albumin as a standard. Absorbance was recorded at 595 nm. All enzymatic assays were carried out at 22±0.5°C using a Specol 11 spectrophotometer (Carl Zeiss Jena, Germany) in duplicate. The enzymatic reactions were started by the addition of the tissue supernatant. Our results demonstrated that immunization by the anti-Yersinia vaccine does not alter the gills of rainbow trout. Oxidative stress parameters examined in gills homogenate, i.e., lipid peroxidation as measured by the amount of TBARS, as well as aldehydic (increased by 18.9%) and ketonic derivatives of OMP (decreased by 6.5 %) were non-significantly changed (p>0.05) in gills of vaccinated fish. Thus, immunization by anti-Yersinia vaccine does not alter oxidative stress markers compared to unhandled control in the second month after immunization. Our results confirm that the vaccine against Y. ruckeri has no adverse effect on the condition and metabolism in the gills of the fish. Alterations in the content of oxidative stress biomarkers recorded in our studies are proof that the vaccine against Y. ruckeri has no negative effects.

scholarly journals Antioxidant responses and plasma biochemical characteristics in the freshwater rainbow trout, Oncorhynchus mykiss, after acute exposure to the fungicide propiconazole

2011 ◽  
Vol 56 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Z.-H. Li ◽  
V. Zlabek ◽  
J. Velíšek ◽  
R. Grabic ◽  
J. Machová ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Control Group ◽  
Antioxidant Responses ◽  
Stress Indices ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Plasma Enzymes ◽  
Plasma Characteristics ◽  
The Brain

In this study, the toxic effects of PCZ, a triazole fungicide present in aquatic environment, were studied in rainbow trout, Oncorhynchus mykiss, by an acute toxicity test. Compared to the control group, fish exposed to PCZ (96-h-LC50, 5.04 mg/l) showed significantly higher (P &lt; 0.05) plasma NH3 and GLU concentration and the activities of plasma enzymes including CK, ALT, AST, LDH, but the TP content was not significantly different (P &gt; 0.05). The oxidative stress indices (levels of LPO and CP) of brain and muscle in the experimental group were higher compared to the control group, especially for a significant change (P &lt; 0.05) in the brain. SOD, CAT, GPx and GR activity in the brain of experimental groups was significantly lower (P &lt; 0.05), however, an opposite tendency was found out in muscle. In addition, there are significant correlations between TBARS and CAT, TBARS and GPx, CP, and CAT, GR, and GPx in the fish brain. Thus, PCZ exposure changed the oxidative stress indices and plasma characteristics, and these changes may be used as potential bioindicators of the exposure and effect of PCZ in the controlled experiment. The use in monitoring of PCZ exposure under natural field conditions is possible, but it needs further investigations.

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