Determination of Chronic Median Lethal Concentration of Sodium Fluoride in Drosophila melanogaster and Exploring Effect of Sub-lethal Concentrations on Differential Hemocyte Count

2017 ◽  
Vol 72 (2) ◽  
pp. 111-117 ◽  
Author(s):  
Moumita Dutta ◽  
Prem Rajak ◽  
Sumedha Roy
Keyword(s):  
Drosophila Melanogaster ◽  
Sodium Fluoride ◽  
Lethal Concentration ◽  
Median Lethal Concentration ◽  
Hemocyte Count

scholarly journals Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Sidra Perveen ◽  
Shalu Kumari ◽  
Himali Raj ◽  
Shahla Yasmin
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Drosophila Melanogaster ◽  
Sodium Fluoride ◽  
Ocimum Sanctum ◽  
Activity Assay ◽  
Lipid Peroxidation Assay ◽  
Climbing Ability ◽  
The Impact ◽  
Sublethal Concentrations

Abstract Background Fluoride may induce oxidative stress and apoptosis. It may also lead to neurobehavioural defects including neuromuscular damage. The present study aimed to explore the effects of sub lethal concentrations of sodium fluoride (NaF) on the lifespan and climbing ability of Drosophila melanogaster. In total, 0.6 mg/L and 0.8 mg/L of NaF were selected as sublethal concentrations of NaF for the study. Lifespan was measured and climbing activity assay was performed. Results The study showed significant decrease in lifespan of flies treated with fluoride. With increasing age, significant reduction in climbing activity was observed in flies treated with sodium fluoride as compared to normal (control) flies. Flies treated with tulsi (Ocimum sanctum) and NaF showed increase in lifespan and climbing activity as compared to those treated with NaF only. Lipid peroxidation assay showed significant increase in malondialdehyde (MDA) values in the flies treated with NaF as compared to control. The MDA values decreased significantly in flies treated with tulsi mixed with NaF. Conclusions The results indicate that exposure to sub lethal concentration of NaF may cause oxidative stress and affect the lifespan and climbing activity of D. melanogaster. Tulsi extract may help in reducing the impact of oxidative stress and toxicity caused by NaF.

scholarly journals Determination of lethal concentration and antibacterial activity of commonly used disinfectants

1970 ◽  
Vol 1 (4) ◽  
pp. 102-105 ◽  
Author(s):  
M Alam ◽  
MM Rahman ◽  
MJ Foysal ◽  
MN Hossain
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Fluorescens ◽  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Pathogenic Bacteria ◽  
Labeo Rohita ◽  
Inhibitory Effect ◽  
Lethal Concentration

The toxic effects of four disinfectants viz., copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green on fish and fish pathogenic bacteria Aeromonas sp., Pseudomonas fluorescens, Edwardsiella sp. and Flavobacterium sp. were investigated. Lethal concentration of the disinfectants to fingerlings of Labeo rohita was determined in aquarium by standard method. Lethal concentration of copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green against fish were found in 0.75ppm, 7ppm, 6ppm and 0.5ppm at 21.4hrs, 18hrs, 9.5hrs and 1.40hrs, respectively. Methylene blue at 4ppm and 5ppm concentration inhibited the growth of Pseudomonas fluorescens and 6ppm concentration suppressed the growth of Aeromonas sp. Copper sulfate (CuSO4) was effective only against Edwardsiella sp at concentration of 10ppm and 8ppm. Malachite green repressed the growth of all four tasted bacteria at a concentration of 1ppm. Potassium permanganate (KMnO4) was failed to exhibit any inhibitory effect on the bacteria even at 30ppm concentration. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9738 IJNS 2011 1(4): 102-105

Acute toxicity and accumulation of microcystin-leucine-arginine in the crayfish Procambarus clarkii (Girard, 1852)

Crustaceana ◽  
2015 ◽  
Vol 88 (4) ◽  
pp. 397-404 ◽  
Author(s):  
Zhenhua An ◽  
Zhenhua An ◽  
Longsheng Sun ◽  
Zhenhua An ◽  
Longsheng Sun ◽  
...  
Keyword(s):  
Survival Rate ◽  
Acute Toxicity ◽  
Procambarus Clarkii ◽  
Abdominal Muscle ◽  
Water Bodies ◽  
Lethal Concentration ◽  
Median Lethal Concentration ◽  
Toxicity Effect ◽  
Cyanobacteria Blooms

The aim of this study was to determine the acute toxicity effect and the accumulation of microcystin-leucine-arginine (MC-LR) on the crayfish Procambarus clarkii (Girard, 1852). Juvenile P. clarkii (5.47 ± 1.3 g) were cultured under 5 different MC-LR concentrations (0.3, 0.6, 1.2, 2.4, 4.8 mg/l), then the acute toxicity effect was observed. The median lethal concentration (LC50) of MC-LR on juvenile P. clarkii was 3.741 mg/l at 24 h, 1.494 mg/l at 48 h, 0.817 mg/l at 72 h and 0.567 mg/l at 96 h. Accumulation of MC-LR was measured in different organs of mature P. clarkii (58.7 ± 3.8 g) exposed to 0.3 mg/l MC-LR for 120 h. The detected MC-LR concentration decreased in the sequence: hepatopancreas > ovary > abdominal muscle > intestine. Hepatopancreas and ovary were found to be the main targets of the toxin. The results suggested that the MC-LR produced by cyanobacteria blooms could not only reduce the survival rate of juvenile P. clarkii but also affect the fecundity of mature crayfish. This research also provides a reference basis for the detection and assessment of the pollution of water bodies in P. clarkii culture.

Group fractionation and determination of the number of ribosomal subunit proteins from Drosophila melanogaster embryos

Biochemistry ◽  
1980 ◽  
Vol 19 (7) ◽  
pp. 1425-1433 ◽  
Author(s):  
W. Yean Chooi ◽  
Linda M. Sabatini ◽  
Elizabeth Dolliver ◽  
Michael Macklin ◽  
Dean Fraser
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Subunit

scholarly journals Determination of the effects of two feed supplements on Drosophila melanogaster

2018 ◽  
Vol 13 (4) ◽  
Author(s):  
Sibele Marques Bolson ◽  
Rodrigo Paidano Alves ◽  
Filipe De Carvalho Victoria ◽  
Kaenara Gomes Munhoz ◽  
Jeferson Luis Franco ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Feed Supplements

scholarly journals Fumigant toxicity of some essential oils against Red Flour Beetles, Tribolium castaneum (Herbst) and its safety to mammals

2020 ◽  
Vol 80 (4) ◽  
pp. 769-776
Author(s):  
T. M. Sileem ◽  
A. L. Mehany ◽  
R. S. Hassan
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Larval Stage ◽  
Adult Stage ◽  
Lethal Concentration ◽  
Median Lethal Concentration ◽  
Histological Changes ◽  
Garlic Oil ◽  
Garlic Essential Oil

Abstract Fumigant activities for three essential oils; Garlic oil (Allium sativum L); Chili pepper (Capsicum annuum L.) and Nigella (Nigella sativa L.) were assessed at different concentrations against the adult and 20-days old larval stages of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) in the laboratory. The accumulative mortality was observed at different exposure periods (3, 5 and 7 days). The residual effect of garlic oil that was the effective oil, on the treated wheat grains was evaluated with respect to histological changes in the liver, kidney, and stomach of rat fed on this treated wheat. The results showed that the mortality rates of treated stages increased with increasing the time of fumigation treatment. Moreover the highest essential oils toxicity at the Median lethal concentration (LC50) values for exposure periods (3, 5 and 7 days) to fumigation were (126, 53, and 47 mg/L air) for adult stage and were (79, 62, and 41 mg/L air) for larval stage, respectively in the case of Garlic oil treatment. While, the lowest essential oils effective was Nigella oil at the Median lethal concentration (LC50) values for exposure periods (3, 5 and 7 days) to fumigation were (3594, 629, and 335 mg/L air) for adult stage and were (1040, 416, and 227 mg/L air) for larval stage, respectively. The toxicity effect of various essential oils against adults and larvae of T. castaneum at the LC50at 7 days fumigation could be arranged in descending order as follows: Garlic oil, Chili pepper oil, and Nigella oil. The histological changes showed that the organs slightly affected at the fumigation for 3 days. It may be concluded that the garlic essential oil is the good effective fumigant to control T. castaneum in the stored products and it recommended that the fumigation period does not exceed 3 days. The garlic essential oil has the potential for applications in IPM programs for stored-grain pests because of its high volatility and fumigant activity and its safety.

The Susceptibility of various Species of Mosquitos to DDT, Dieldrin and BHC

1955 ◽  
Vol 46 (2) ◽  
pp. 301-309 ◽  
Author(s):  
R. H. Wharton
Keyword(s):  
Aedes Aegypti ◽  
Culex Pipiens ◽  
Lethal Concentration ◽  
Median Lethal Concentration ◽  
Adult Females ◽  
Anopheles Maculipennis ◽  
Culex Pipiens Molestus ◽  
Filter Papers

The susceptibility of various mosquitos to DDT, dieldrin and BHC has been studied. Adult females were exposed in small tubes lined with filter papers impregnated with insecticide-oil solutions. Larvae were exposed to insecticideacetone suspensions in water.Adult mosquitos tested in London showed no striking differences in susceptibility to DDT, though the median lethal concentration (MLC) of Culex pipiens molestus Forsk. (1·6 per cent.) was greater than the MLC of Aëdes aegypti (L.) (0·9), Anopheles maculipennis var. atroparvusvan Thiel (1·0), or A. quadrimaculatus Say (·7). C. p. molestus and Aë. aegypti showed the same order of susceptibility to dieldrin and BHC, with Anopheles m. atroparvus more susceptible to both insecticides.

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