Effect of Lysimachia ramosa Wall. Ex Duby and Its n-Butanol Fraction on Glycogen Content and Some Energy Related Enzymes in the Cestode, Raillietina echinobothrida

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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ÉTUDE IN VITRO DU MÉTABOLISME DES HYDRATES DE CARBONE DANS LE LEUCOCYTE CIRCULANT DU COBAYE TRAITÉ À LA CORTISONE

Acta Endocrinologica ◽

10.1530/acta.0.0510193 ◽

1966 ◽

Vol 51 (2) ◽

pp. 193-202

Author(s):

J. A. Antonioli ◽

A. Vannotti

Keyword(s):

Glucose Concentration ◽

Intramuscular Injection ◽

Acute Inflammation ◽

Glycogen Content ◽

Glycogen Synthesis ◽

Lactate Production ◽

Glucose Consumption ◽

List Type ◽

Hydrates De Carbone

ABSTRACT 1. The metabolism of suspensions of circulating leucocytes has been studied after intramuscular injection of a dose of 50 mg/kg of a corticosteroid (cortisone acetate). The suspensions were incubated under aerobic conditions in the presence of a glucose concentration of 5.6 mm. Glucose consumption, lactate production, and variations in intracellular glycogen concentration were measured. After the administration of the corticosteroid, the anabolic processes of granulocyte metabolism were reversibly stimulated. Glucose consumption and lactate production increased 12 hours after the injection, but tended to normalize after 24 hours. The glycogen content of the granulocytes was enhanced, and glycogen synthesis during the course of the incubation was greatly stimulated. The action of the administered corticosteroid is more prolonged in females than in males. The injection of the corticosteroid caused metabolic modifications which resemble in their modulations and in their chronological development those found in circulating granulocytes of guinea-pigs suffering from sterile peritonitis. These results suggest, therefore, that, in the case of acute inflammation, the glucocorticosteroids may play an important role in the regulation of the metabolism of the blood leucocytes.

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Hypothalamic HSP10 Impacts Mitochondrial Dynamics, Insulin Signaling, and Liver Glycogen Content

Diabetes ◽

10.2337/db18-1801-p ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 1801-P

Author(s):

KRISTINA WARDELMANN ◽

JOSÉ PEDRO CASTRO ◽

MICHAELA RATH ◽

JÜRGEN WEIß ◽

ANNETTE SCHUERMANN ◽

...

Keyword(s):

Insulin Signaling ◽

Glycogen Content ◽

Mitochondrial Dynamics ◽

Liver Glycogen ◽

Liver Glycogen Content

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METHODOLOGICAL GUIDELINES FOR EVALUATION OF HELMINTHOZOONOSIS LARVAE INVASIVENESS IN TERMS OF GLYCOGEN CONTENT

10.31016/vniip-2019-1 ◽

2019 ◽

Author(s):

O.N. Andreyanov ◽

◽

E.A. Sidor ◽

A.N. Postevoy ◽

A.V. Uspensky ◽

...

Keyword(s):

Glycogen Content ◽

Methodological Guidelines

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Placental G6Pase and glycogen content in normal pregnancy and small for gestational age pregnancies

Archives of Disease in Childhood - Fetal and Neonatal Edition ◽

10.1136/adc.2010.189746.38 ◽

2010 ◽

Vol 95 (Supplement 1) ◽

pp. Fa22-Fa22

Author(s):

J. Leonce ◽

N. Brockton ◽

A. Burchell ◽

S. Robinson ◽

P. Steer ◽

...

Keyword(s):

Gestational Age ◽

Small For Gestational Age ◽

Glycogen Content ◽

Normal Pregnancy

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Conversion of Spent Coffee and Donuts by Black Soldier Fly (Hermetia illucens) Larvae into Potential Resources for Animal and Plant Farming

Insects ◽

10.3390/insects12040332 ◽

2021 ◽

Vol 12 (4) ◽

pp. 332

Author(s):

Hayden Fischer ◽

Nicholas Romano ◽

Amit Kumar Sinha

Keyword(s):

Organic Waste ◽

Glycogen Content ◽

Organic Fertilizer ◽

Organic Fertilizers ◽

Black Soldier Fly ◽

Hermetia Illucens ◽

Hermetia Illucens Larvae ◽

Nitrogen Phosphorus ◽

Dough Stage ◽

Plant Farming

Nutritionally unbalanced organic waste can be converted into potential resources for animal and plant farming by culturing black soldier fly (Hermetia illucens) larvae (BSFL) and prepupae (BSFP). BSFL and BSFP are rich sources of protein and lipids, while the leftover excrement called “frass” can be used as an organic fertilizer. Using readily available resources, BSFL were cultured on spent coffee, donut dough or an equal blend for 35 days. Survival, productivity, daily pupation and biochemical composition of BSFL and BSFP were measured along with the nitrogen-phosphorus-potassium values of the frass. Survival was highest in the blend compared (81%) to spent coffee (45%) or dough (24%); however, BSFL and BSFP were significantly longer and heavier from dough. Stage and food significantly influenced the protein, lipid and glycogen content of the BSFL and BSFP, which tended to be higher in the latter. While fatty acids were often significantly higher in BSFL fed spent coffee, the amino acid composition of BSFL was generally higher in dough. Frass from the blend had significantly highest nitrogen content, while potassium and phosphorus were significantly higher and lower from spent coffee, respectively. Although coffee and donut dough were suboptimal substrates for BSFL, a blend of these produced BSFL and frass that were nutritionally comparable to soybean meal and many organic fertilizers, respectively.

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Postprandial Glycogen Content Is Increased in the Hepatocytes of Human and Rat Cirrhotic Liver

Cells ◽

10.3390/cells10050976 ◽

2021 ◽

Vol 10 (5) ◽

pp. 976

Author(s):

Natalia N. Bezborodkina ◽

Sergey V. Okovityi ◽

Boris N. Kudryavtsev

Keyword(s):

Rat Liver ◽

Liver Tissue ◽

Glycogen Content ◽

Glycogen Synthase ◽

Fibrous Tissue ◽

Glycogen Metabolism ◽

Liver Parenchyma ◽

Dry Mass ◽

Cirrhotic Liver ◽

Liver Biopsies

Chronic hepatitises of various etiologies are widespread liver diseases in humans. Their final stage, liver cirrhosis (LC), is considered to be one of the main causes of hepatocellular carcinoma (HCC). About 80–90% of all HCC cases develop in LC patients, which suggests that cirrhotic conditions play a crucial role in the process of hepatocarcinogenesis. Carbohydrate metabolism in LC undergoes profound disturbances characterized by altered glycogen metabolism. Unfortunately, data on the glycogen content in LC are few and contradictory. In this study, the material was obtained from liver biopsies of patients with LC of viral and alcohol etiology and from the liver tissue of rats with CCl4-induced LC. The activity of glycogen phosphorylase (GP), glycogen synthase (GS), and glucose-6-phosphatase (G6Pase) was investigated in human and rat liver tissue by biochemical methods. Total glycogen and its labile and stable fractions were measured in isolated individual hepatocytes, using the cytofluorometry technique of PAS reaction in situ. The development of LC in human and rat liver was accompanied by an increase in fibrous tissue (20- and 8.8-fold), an increase in the dry mass of hepatocytes (by 25.6% and 23.7%), and a decrease in the number of hepatocytes (by 50% and 28%), respectively. The rearrangement of the liver parenchyma was combined with changes in glycogen metabolism. The present study showed a significant increase in the glycogen content in the hepatocytes of the human and the rat cirrhotic liver, by 255% and 210%, respectively. An increased glycogen content in cells of the cirrhotic liver can be explained by a decrease in glycogenolysis due to a decreased activity of G6Pase and GP.

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Glycogen content is affected differently in acute pulmonary and extra-pulmonary lung injury

Human & Experimental Toxicology ◽

10.1177/0960327109106998 ◽

2009 ◽

Vol 28 (9) ◽

pp. 583-590 ◽

Cited By ~ 4

Author(s):

EL Borges ◽

MB Pinheiro ◽

A. Eleto-Silva ◽

MV Caliari ◽

MG Rodrigues-Machado

Keyword(s):

Lung Injury ◽

Gastrocnemius Muscle ◽

Glycogen Content ◽

Inflammatory Cells ◽

Severe Form ◽

Control Groups ◽

Biochemical Aspect ◽

Extra Pulmonary ◽

Anthrone Reagent ◽

Different Tissues

Acute respiratory distress syndrome (ARDS) is the most severe form of acute lung injury (ALI). The aim of the present study was to investigate whether paraquat-induced acute pulmonary and extra-pulmonary lung injury (ALI-P and ALI-EX, respectively), in rats, affects glycogen content in different tissues. This measurement could indicate performance limitations of tissues, a new biochemical aspect of ARDS. ALI-P and ALI-EX were induced by injection into the trachea (0.5 mg/kg) and intraperitoneally (20 mg/kg) 24 hours prior to tissue collection. The control groups (CTRL) received the same volume of saline. Glycogen content (mg/g tissue) from different tissues was measured using the anthrone reagent. Glycogen content in the heart and kidney was higher in the ALI-EX group than the CTRL-EX group. Glycogen content in the gastrocnemius muscle was lower in the ALI-EX group than the CTRL-EX group. However, there were no significant differences in glycogen content in the diaphragm in the ALI-EX and ALI-P groups or in the gastrocnemius, heart and kidney in the ALI-P group when compared to the respective controls. ALI-EX caused a greater thickening of the alveolar walls, more areas of atelectasis and a greater abundance of inflammatory cells in comparison to ALI-P. These results demonstrate that glycogen content in ALI, induced by an herbicide that is highly toxic to humans and animals, is altered in different tissues depending on the location of the injury.

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