Glycogen content is affected differently in acute pulmonary and extra-pulmonary lung injury

Acute respiratory distress syndrome (ARDS) is the most severe form of acute lung injury (ALI). The aim of the present study was to investigate whether paraquat-induced acute pulmonary and extra-pulmonary lung injury (ALI-P and ALI-EX, respectively), in rats, affects glycogen content in different tissues. This measurement could indicate performance limitations of tissues, a new biochemical aspect of ARDS. ALI-P and ALI-EX were induced by injection into the trachea (0.5 mg/kg) and intraperitoneally (20 mg/kg) 24 hours prior to tissue collection. The control groups (CTRL) received the same volume of saline. Glycogen content (mg/g tissue) from different tissues was measured using the anthrone reagent. Glycogen content in the heart and kidney was higher in the ALI-EX group than the CTRL-EX group. Glycogen content in the gastrocnemius muscle was lower in the ALI-EX group than the CTRL-EX group. However, there were no significant differences in glycogen content in the diaphragm in the ALI-EX and ALI-P groups or in the gastrocnemius, heart and kidney in the ALI-P group when compared to the respective controls. ALI-EX caused a greater thickening of the alveolar walls, more areas of atelectasis and a greater abundance of inflammatory cells in comparison to ALI-P. These results demonstrate that glycogen content in ALI, induced by an herbicide that is highly toxic to humans and animals, is altered in different tissues depending on the location of the injury.

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Effects of sepsis-induced acute lung injury on glycogen content in different tissues

Experimental Lung Research ◽

10.3109/01902141003609983 ◽

2010 ◽

Vol 36 (5) ◽

pp. 302-306 ◽

Cited By ~ 5

Author(s):

Maria da Glória Rodrigues-Machado ◽

Grazielle C. Silva ◽

Marina B. Pinheiro ◽

Marcelo V. Caliari ◽

Elizabeth L. Borges

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Glycogen Content ◽

Different Tissues

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Metalloproteinase and growth factor interactions: do they play a role in pulmonary fibrosis?

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00489.2001 ◽

2002 ◽

Vol 283 (1) ◽

pp. L1-L11 ◽

Cited By ~ 26

Author(s):

Margaret K. Winkler ◽

John L. Fowlkes

Keyword(s):

Acute Lung Injury ◽

Growth Factors ◽

Growth Factor ◽

Pulmonary Fibrosis ◽

Lung Injury ◽

Transforming Growth Factor ◽

Interstitial Fibrosis ◽

Inflammatory Cells ◽

Transforming Growth Factor Β ◽

Target Cells

Chronic lung disease due to interstitial fibrosis can be a consequence of acute lung injury and inflammation. The inflammatory response is mediated through the migration of inflammatory cells, actions of proinflammatory cytokines, and the secretion of matrix-degrading proteinases. After the initial inflammatory insult, successful healing of the lung may occur, or alternatively, dysregulated tissue repair can result in scarring and fibrosis. On the basis of recent insights into the mechanisms underlying acute lung injury and its long-term consequences, data suggest that proteinases, such as the matrix metalloproteinases (MMPs), may not only be involved in the breakdown and remodeling that occurs during the injury but may also cause the release of growth factors and cytokines known to influence growth and differentiation of target cells within the lung. Through the release of and activation of fibrosis-promoting cytokines and growth factors such as transforming growth factor-β1, tumor necrosis factor-α, and insulin-like growth factors by MMPs, we propose that these metalloproteinases may be integral to the initiation and progression of pulmonary fibrosis.

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Vitamin A deficiency enhances ozone-induced lung injury

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1996.270.3.l475 ◽

1996 ◽

Vol 270 (3) ◽

pp. L475-L482 ◽

Cited By ~ 2

Author(s):

N. C. Paquette ◽

L. Y. Zhang ◽

W. A. Ellis ◽

A. L. Scott ◽

S. R. Kleeberger

Keyword(s):

Lung Injury ◽

Vitamin A ◽

Total Protein ◽

High Performance ◽

Vitamin A Deficiency ◽

Inflammatory Cells ◽

Inbred Strains ◽

Retinyl Palmitate ◽

Differential Cell Count ◽

Inbred Strains Of Mice

The present study determined the effects of vitamin A (vA) deficiency on the responses to ozone (O3) challenges in two inbred strains of mice that are differentially susceptible to O3-induced lung inflammation. Susceptible C57BL/6J (B6) and resistant C3H/HeJ (C3) dams at 2 wk gestation were fed test diets containing either 0 or 10 micrograms retinol/g diet. In mice that were maintained on vA-sufficient (vA+) diet, lung and liver tissue concentrations of vA and retinyl palmitate (RP) were significantly (P<0.05) lower in the B6 strain compared with C3, as measured by high-performance liquid chromatography techniques. vA and RP levels were significantly (P<0.05) reduced in lung and liver tissues of 8-wk old B6 and C3 mice that were maintained on a vA deficient (vA-) diet. vA+ and vA- mice of both strains were exposed to air or 0.3 ppm O3/72 h, and lung injury was assessed by differential cell count and total protein concentration in bronchoalveolar lavage (BAL) returns. O3 exposure caused significantly (P<0.05) greater increases in inflammatory cells and a total protein in BAL returns of vA+ B6 mice than vA+ C3 mice. vA deficiency significantly (P<0.05) enhanced O3-induced increases in polymorphonuclear leukocytes in C3 mice and epithelial cells loss in both strains. Compared with vA+ mice, lung permeability was also significantly (P<0.05) enhanced in vA- mice of both strains exposed to O3. vA replacement partially reversed the O3-induced lung injury that was enhanced by vA- diet. Results indicate that vA may have an important role in the pathogenesis of O3-induced lung injury in differentially susceptible inbred strains of mice.

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Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

Journal of Baghdad College of Dentistry ◽

10.26477/jbcd.v31i4.2724 ◽

2019 ◽

Vol 31 (4) ◽

Author(s):

Nawar Bahjet Kamil ◽

Nada M. Al-Ghaban

Keyword(s):

Healing Process ◽

Inflammatory Cells ◽

Control Group ◽

Facial Skin ◽

Histological Findings ◽

Control Groups ◽

Mean Values ◽

Significant Difference ◽

The Right ◽

New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

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YiQiFuMai Lyophilized Injection Attenuates Sepsis-Induced Acute Lung Injury via TLR4/Src/VE-cadherin/p120-catenin Signaling Pathway

10.21203/rs.3.rs-63630/v1 ◽

2020 ◽

Author(s):

Xue-wei Pan ◽

Li-xuan Xue ◽

Qian-liu Zhou ◽

Jia-zhi Zhang ◽

Yu-jie Dai ◽

...

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Signaling Pathway ◽

Therapeutic Potential ◽

Cecal Ligation ◽

Inflammatory Cells ◽

Underlying Mechanism ◽

Lavage Fluid ◽

Lung Edema ◽

P120 Catenin

Abstract Background: Sepsis is a severe disorder leading to a clinically critical syndrome of multiple organ dysfunction syndrome. Most patients with sepsis will be associated with acute lung injury (ALI), which is an independent risk factors of organ failure and death in patients with sepsis at the same time. YiQiFuMai Lyophilized Injection (YQFM) is a modern traditional Chinese prescription preparation, which could ameliorate ALI induced by lipopolysaccharide (LPS) or fine particulate matter. The current study aimed to investigate the effect of YQFM on sepsis-induced ALI and the underlying mechanism.Methods: Male C57BL/6J mice were treated with cecal ligation and puncture (CLP) after tail intravenous injected with YQFM (1, 2 and 4 g/kg). The measurements of lung edema, evans blue leakage, myeloperoxidase content, inflammatory cells in bronchoalveolar lavage fluid, histopathological assay and expression of associated proteins were performed at 18 h after CLP.Results: The results illustrated that YQFM inhibited pulmonary edema and inflammatory response, thus ameliorated ALI in sepsis mice. Furthermore, the expression of TLR4 and phosphorylated Src was down-regulated, and the expression of p120-catenin and VE-cadherin was restored by YQFM administration.Conclusion: Our study suggested the therapeutic potential of YQFM on treating sepsis-induced ALI via regulating TLR4/Src/VE-cadherin/p120-catenin signaling pathway.

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Leucine-Rich Diet and Light Aerobic Training Modulate GLUT4 Expression And Increase the Glycogen Content in the Gastrocnemius Muscle of Walker-256 Tumour-Bearing Rats

Journal of Physiobiochemical Metabolism ◽

10.4172/2324-8793.1000112 ◽

2014 ◽

Vol 03 (01) ◽

Author(s):

Aline T Toneto

Keyword(s):

Gastrocnemius Muscle ◽

Glycogen Content ◽

Aerobic Training ◽

Tumour Bearing ◽

Glut4 Expression ◽

Walker 256 Tumour ◽

Walker 256

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Histological analysis of the biocompatibility of three different calcium hydroxide-based root canal sealers

Journal of Applied Oral Science ◽

10.1590/s1678-77572006000500014 ◽

2006 ◽

Vol 14 (5) ◽

pp. 376-381 ◽

Cited By ~ 7

Author(s):

Heloisa Helena Pinho Veloso ◽

Roberto Alves do Santos ◽

Túlio Pessoa de Araújo ◽

Denise Piotto Leonardi ◽

Flares Baratto Filho

Keyword(s):

Calcium Hydroxide ◽

Study Design ◽

Inflammatory Reaction ◽

Root Canal ◽

Histological Analysis ◽

Subcutaneous Tissue ◽

Inflammatory Cells ◽

Control Groups ◽

Tissue Reactions ◽

Root Canal Sealers

OBJECTIVE: To histologically evaluate the behavior of the subcutaneous tissue of rats after placement of polyethylene tubes filled with calcium hydroxide-based root canal sealers (SealapexTM, ApexitTM e Sealer 26TM). STUDY DESIGN: The study employed 60 rats, which were divided into eight groups, being four experimental groups with 10 rats each, and four control groups with five rats. Analysis was conducted at 48 hours, 7, 21 and 60 days. RESULTS: Histological analysis revealed that all sealers were irritant; however, the intensity varied between each sealer and as a function of time. At the initial periods (48 hours and seven days), all materials demonstrated similar outcomes, with inflammatory reaction from severe to moderate, respectively. At the final periods (21 and 60 days), the tissue reactions to the implants containing SealapexTM displayed an active status yet with regression, compared to the ApexitTM and Sealer 26TM. The control groups exhibited less inflammatory cells than the experimental groups, keeping a reaction status with regression. CONCLUSIONS: The results achieved in this study revealed that the SealapexTM sealer was the most compatible, followed by ApexitTM and Sealer 26TM.

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