scholarly journals In vitro tegumental alterations on adult Fasciola gigantica caused by mefloquine

2014 ◽  
Vol 40 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Hatem A. Shalaby ◽  
Amira H. El Namaky ◽  
Reem O. A. Kamel
Keyword(s):  
Fasciola Gigantica

In vitro effect of artemether and triclabendazole on adult Fasciola gigantica

2009 ◽  
Vol 160 (1-2) ◽  
pp. 76-82 ◽  
Author(s):  
Hatem A. Shalaby ◽  
Amira H. El Namaky ◽  
Reem O.A. Kamel
Keyword(s):  
Fasciola Gigantica ◽  
Vitro Effect

scholarly journals Gamal (Gliricidia Sepium) Leaf Extract Increase Mortality of Fasciola gigantica In Vitro

2021 ◽  
Vol 4 (2) ◽  
pp. 193
Author(s):  
Brillia Zulianti ◽  
Iwan Sahrial Hamid ◽  
Aditya Yudhana ◽  
Jola Rahmahani ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Gliricidia Sepium ◽  
Fasciola Gigantica ◽  
Economic Losses ◽  
Randomized Design ◽  
The Mean ◽  
Range Test

Fasciolosis is a disease caused by infection with liver fluke (Fasciola sp). Fasciolosis is a parasitic disease that is important because it can cause high economic losses. The aim of this study was to investigate the effect of gamal (Gliricidia sepium) leaf extract against mortality of Fasciola gigantica in vitro study. In present study was completely randomized design with six treatments and each treatment were divided into four replications. A total of 240 specimens from infected cattles were used in this study. The observation and recording of F. gigantica mortality in all treatments with concentrations of gamal leaf extract P1 (1%), P2 (2%), P3 (3), P4 (4%), K + (Nitroxynil 2%) and K- (physiological NaCl) rate was done in 30, 60, 90, 120, 150, 180, 210 and 240 minutes, respectively. The mortality of F. gigantica was confirmed if there was no movement when triggered by anatomy tweezers then dipped in slightly warm water (50 15℃"> ). The data was analyzed using ANAVA Faktorial and continued with Duncan Multiple Range Test. The results of this study were shown the lowest and the highest mortality rate recorded at minutes 30 and 240 respectively. Moreover, the mean of mortality rate were peaked in nitroxynil (K+) and 1% gamal leaf extract treatment, while the lowest mean of mortality rate occurs in NaCl (K-) treatment. Present study conclude that gamal leaf extract has anthelmintic effect on the mortality rate of Fasciola gigantica. Gamal leaf extract with 1% concentration was claimed to be the most effective.

scholarly journals Modulation of the Functions of Goat Peripheral Blood Mononuclear Cells by Fasciola gigantica Thioredoxin Peroxidase In Vitro

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 758 ◽  
Author(s):  
Ai-Ling Tian ◽  
Xiaowei Tian ◽  
Dan Chen ◽  
Mingmin Lu ◽  
Guillermo Calderón-Mantilla ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Transforming Growth Factor ◽  
Interleukin 2 ◽  
Fasciola Gigantica ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Thioredoxin Peroxidase

The liver fluke Fasciola gigantica has a remarkable ability to establish a long-term infection within the hepatobiliary system of the mammalian definitive host. F. gigantica achieves this by producing excretory–secretory molecules, which have immunomodulatory activities. In an effort to elucidate the immunomodulatory functions of F. gigantica thioredoxin peroxidase protein (FgTPx), we expressed recombinant FgTPx (rFgTPx) in Escherichia coli bacteria and examined its effects on several functions of goat peripheral blood mononuclear cells (PBMCs) in vitro. Sequence analysis revealed that FgTPx is related to a thioredoxin-like superfamily. Western blot analysis showed that rFgTPx was recognized by the sera of goats experimentally infected by F. gigantica. The specific binding of rFgTPx protein to the surface of goat PBMCs was demonstrated by immunofluorescence staining. We investigated the influence of serial concentrations of rFgTPx on various functions of goat PBMCs. All concentrations of rFgTPx increased the secretion of interleukin-2 (IL-2), IL-4, IL-10, IL-17, transforming growth factor-beta (TGF-β), and interferon gamma (IFN-γ), but inhibited PBMC proliferation, migration, and monocyte phagocytosis. Goat PBMCs exposed to 20–40 μg/mL of rFgTPx secreted increased levels of nitric oxide (NO), and 10–40 μg/mL of rFgTPx promoted cell apoptosis. These findings indicate that rFgTPx influences various functions of goat PBMCs by interacting with a large number of cellular targets, ultimately to promote the parasite’s survival. The roles of rFgTPx and their interacting proteins warrant further investigation.

In-vitro and in-vivo assessment of the effect of soybean extract on Fasciola gigantica infection in comparison with triclabendazole

2014 ◽  
Vol 27 (1) ◽  
pp. 93 ◽  
Author(s):  
SamarA El Refai Khalil ◽  
NashaatE. Nassef ◽  
WafaaM. El-Kersh ◽  
MonaM El Sobky ◽  
NancyM. Harba
Keyword(s):  
Fasciola Gigantica ◽  
Soybean Extract ◽  
In Vivo Assessment

The effect of five fasciolicides on malate dehydrogenase activity and mortality of Fasciola gigantica, Fasciolopsis buski and Paramphistomum explanatum

1981 ◽  
Vol 55 (2) ◽  
pp. 115-122 ◽  
Author(s):  
A. J. Probert ◽  
R. K. Sharma ◽  
K. Singh ◽  
R. Saxena
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Malate Dehydrogenase ◽  
Fasciola Gigantica ◽  
High Concentrations ◽  
Fasciolopsis Buski ◽  
Reduced Activity ◽  
Inhibited Enzyme ◽  
Malate Oxidation

ABSTRACTThe effect of oxyclozanide, hexachlorophene, nitroxynil, rafoxanide and diamphenethide on malate dehydrogenase activity of homogenates of Fasciola gigantica, Fasciolopsis buski and Paramphistomum explanatum was investigated. The ratio of oxaloacetate reduction to malate oxidation in homogenates of Fasciola gigantica, Fasciolopsis buski and P. explanatum was 4·5:1, 3·6:1 and 5·2:1 respectively. Oxyclozanide and rafoxanide at 10−3 M inhibited enzyme activity by 100% in homogenates from all three species while hexachlorophene at 10−3M also caused 100% inhibition in homogenates from Fasciola gigantica and P. explanatum but only 65% of malate oxidation in Fasciolopsis buski homogenates. Nitroxynil at 10−3M produced 60% inhibition in F. buski homogenates yet had little effect at this concentration on preparations from the other species. Little inhibition was seen with diamphenethide, even at high concentrations. Rapid death of Fasicola gigantica and P. explanatum resulted in vitro when 10−3M oxyclozanide, hexachlorophene, nitroxynil or rafoxanide, were added to the incubation medium. Fasciolopsis buski was killed by 10−3M oxyclozanide but at this concentration the remaining compounds only caused reduced activity. Assay of malate dehydrogenase following drug treatment in vitro failed to show any appreciable reduction in enzyme activity in Fasciola gigantica and P. explanatum but oxyclozanide and hexachlorophene produced inhibition in Fasciolopsis buski. The mode of action of these compounds is discussed.

Fasciola gigantica: tegumental surface alterations following treatment in vitro with the sulphoxide metabolite of triclabendazole

2001 ◽  
Vol 88 (4) ◽  
pp. 315-325 ◽  
Author(s):  
M. Meaney ◽  
I. Fairweather ◽  
G. Brennan ◽  
P. Ramasamy ◽  
P. Subramanian
Keyword(s):  
Fasciola Gigantica ◽  
Tegumental Surface

scholarly journals The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro

2021 ◽  
Vol 10 (2) ◽  
pp. 66
Author(s):  
Firanda Agustina ◽  
Rochmah Kurnijasanti ◽  
Lucia Tri Suwanti ◽  
Nunuk Dyah Retno Lastuti ◽  
Kadek Rachmawati ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Immersion Time ◽  
Morphological Changes ◽  
Anthelmintic Activity ◽  
Ethanol Extract ◽  
Fasciola Gigantica ◽  
Histopathological Changes ◽  
Extract Concentration ◽  
Anthelmintic Effect

This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.

Sign in / Sign up

Export Citation Format

Share Document